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The map label for this gene is pksG

Identifier: 50812233

GI number: 50812233

Start: 1789943

End: 1791205

Strand: Direct

Name: pksG

Synonym: BSU17150

Alternate gene names: 50812233

Gene position: 1789943-1791205 (Clockwise)

Preceding gene: 255767394

Following gene: 16078777

Centisome position: 42.46

GC content: 47.03

Gene sequence:

>1263_bases
ATGGTTTCAGCCGGAATAGAAGCGATGAATGTCTTTGGCGGAACGGCATACCTTGATGTCATGGAGCTGGCCAAATACAG
ACACTTAGATACTGCGAGATTTGAAAATTTATTAATGAAAGAAAAAGCGGTAGCACTGCCTTATGAAGATCCCGTGACCT
TCGGAGTCAATGCAGCTAAGCCGATTATTGATGCTTTATCTGAAGCAGAGAAGGACCGGATTGAATTGCTGATCACTTGC
TCTGAATCCGGGATCGACTTTGGGAAATCGTTAAGTACATATATCCATGAATATTTAGGTCTCAACCGCAACTGCCGTTT
GTTTGAGGTTAAACAGGCGTGTTATTCAGGGACAGCGGGATTTCAGATGGCGGTGAATTTTATATTATCCCAGACTTCTC
CGGGTGCCAAAGCACTGGTTATCGCCAGTGATATTTCACGTTTTTTAATCGCTGAGGGCGGAGACGCTTTAAGCGAAGAT
TGGTCTTATGCGGAACCGAGCGCCGGAGCGGGAGCGGTGGCCGTTTTAGTTGGCGAAAATCCCGAGGTGTTTCAAATAGA
TCCCGGAGCAAATGGTTATTACGGCTATGAAGTGATGGACACGTGCCGCCCCATTCCCGACAGTGAAGCGGGAGATTCTG
ATCTGTCTTTAATGTCATATTTGGATTGCTGCGAACAGACTTTTCTGGAATATCAAAAGCGTGTACCGGGTGCCAATTAT
CAGGACACGTTTCAATATCTTGCTTACCATACGCCCTTTGGCGGGATGGTGAAAGGTGCGCACCGGACAATGATGCGTAA
GGTAGCGAAGGTGAAAACATCCGGGATTGAGACGGATTTTTTAACTCGGGTAAAACCAGGTTTAAACTACTGTCAACGGG
TTGGAAATATTATGGGGGCAGCCTTGTTTCTGGCATTGGCCAGCACGATAGACCAAGGGAGATTTGATACGCCTAAACGA
ATTGGCTGTTTTTCCTACGGATCCGGCTGCTGTTCTGAATTTTACAGCGGAATTACAACTCCGCAAGGCCAGGAGCGTCA
GCGAACATTTGGTATTGAGAAGCATTTGGATCGCCGGTATCAGCTCTCCATGGAAGAGTATGAGCTATTGTTTAAGGGGA
GCGGGATGGTGCGGTTTGGGACGCGCAATGTAAAGCTGGACTTTGAGATGATACCGGGCATCATGCAGTCCACTCAAGAG
AAGCCGCGCTTGTTTTTAGAAGAAATTTCTGAGTTTCATCGGAAGTACAGGTGGATCTCGTGA

Upstream 100 bases:

>100_bases
ATACCGCATCAAAAATGCGCTGAGCTTAAGCATGGGGTTTGGCGGAATGAACACCGCGGTTTGTATACAAAACATTGAGA
AATGTGGAGGCGAATCATAA

Downstream 100 bases:

>100_bases
CCTATCAAACGATAAAGGTCCGCTTTCAAGCATCGGTTTGCTATATTACCTTTCACCGTCCTGAAGCGAATAATACGATT
AATGACACGCTGATAGAAGA

Product: acetyl-S-AcpK beta-ketothioester polyketide intermediate transferase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MVSAGIEAMNVFGGTAYLDVMELAKYRHLDTARFENLLMKEKAVALPYEDPVTFGVNAAKPIIDALSEAEKDRIELLITC
SESGIDFGKSLSTYIHEYLGLNRNCRLFEVKQACYSGTAGFQMAVNFILSQTSPGAKALVIASDISRFLIAEGGDALSED
WSYAEPSAGAGAVAVLVGENPEVFQIDPGANGYYGYEVMDTCRPIPDSEAGDSDLSLMSYLDCCEQTFLEYQKRVPGANY
QDTFQYLAYHTPFGGMVKGAHRTMMRKVAKVKTSGIETDFLTRVKPGLNYCQRVGNIMGAALFLALASTIDQGRFDTPKR
IGCFSYGSGCCSEFYSGITTPQGQERQRTFGIEKHLDRRYQLSMEEYELLFKGSGMVRFGTRNVKLDFEMIPGIMQSTQE
KPRLFLEEISEFHRKYRWIS

Sequences:

>Translated_420_residues
MVSAGIEAMNVFGGTAYLDVMELAKYRHLDTARFENLLMKEKAVALPYEDPVTFGVNAAKPIIDALSEAEKDRIELLITC
SESGIDFGKSLSTYIHEYLGLNRNCRLFEVKQACYSGTAGFQMAVNFILSQTSPGAKALVIASDISRFLIAEGGDALSED
WSYAEPSAGAGAVAVLVGENPEVFQIDPGANGYYGYEVMDTCRPIPDSEAGDSDLSLMSYLDCCEQTFLEYQKRVPGANY
QDTFQYLAYHTPFGGMVKGAHRTMMRKVAKVKTSGIETDFLTRVKPGLNYCQRVGNIMGAALFLALASTIDQGRFDTPKR
IGCFSYGSGCCSEFYSGITTPQGQERQRTFGIEKHLDRRYQLSMEEYELLFKGSGMVRFGTRNVKLDFEMIPGIMQSTQE
KPRLFLEEISEFHRKYRWIS
>Mature_420_residues
MVSAGIEAMNVFGGTAYLDVMELAKYRHLDTARFENLLMKEKAVALPYEDPVTFGVNAAKPIIDALSEAEKDRIELLITC
SESGIDFGKSLSTYIHEYLGLNRNCRLFEVKQACYSGTAGFQMAVNFILSQTSPGAKALVIASDISRFLIAEGGDALSED
WSYAEPSAGAGAVAVLVGENPEVFQIDPGANGYYGYEVMDTCRPIPDSEAGDSDLSLMSYLDCCEQTFLEYQKRVPGANY
QDTFQYLAYHTPFGGMVKGAHRTMMRKVAKVKTSGIETDFLTRVKPGLNYCQRVGNIMGAALFLALASTIDQGRFDTPKR
IGCFSYGSGCCSEFYSGITTPQGQERQRTFGIEKHLDRRYQLSMEEYELLFKGSGMVRFGTRNVKLDFEMIPGIMQSTQE
KPRLFLEEISEFHRKYRWIS

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family

Homologues:

Organism=Homo sapiens, GI5031751, Length=419, Percent_Identity=23.8663484486874, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI148298764, Length=383, Percent_Identity=25.5874673629243, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI148298677, Length=383, Percent_Identity=25.5874673629243, Blast_Score=90, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17560126, Length=455, Percent_Identity=23.0769230769231, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323509, Length=413, Percent_Identity=22.2760290556901, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24654139, Length=424, Percent_Identity=23.3490566037736, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654137, Length=424, Percent_Identity=23.3490566037736, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654133, Length=424, Percent_Identity=23.3490566037736, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654135, Length=424, Percent_Identity=23.3490566037736, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17933694, Length=424, Percent_Identity=23.3490566037736, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PKSG_BACSU (P40830)

Other databases:

- EMBL:   U11039
- EMBL:   AL009126
- PIR:   E69678
- RefSeq:   NP_389595.2
- ProteinModelPortal:   P40830
- SMR:   P40830
- EnsemblBacteria:   EBBACT00000000429
- GeneID:   940121
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17150
- NMPDR:   fig|224308.1.peg.1717
- GenoList:   BSU17150
- GeneTree:   EBGT00050000002103
- HOGENOM:   HBG578402
- OMA:   EDWSFAE
- PhylomeDB:   P40830
- ProtClustDB:   CLSK928132
- BioCyc:   BSUB:BSU17150-MONOMER
- GO:   GO:0005737
- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038
- Gene3D:   G3DSA:3.40.47.10

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N; SSF53901 Thiolase-like

EC number: 2.3.3.10

Molecular weight: Translated: 46781; Mature: 46781

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: ACT_SITE 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAGIEAMNVFGGTAYLDVMELAKYRHLDTARFENLLMKEKAVALPYEDPVTFGVNAAK
CCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHH
PIIDALSEAEKDRIELLITCSESGIDFGKSLSTYIHEYLGLNRNCRLFEVKQACYSGTAG
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCH
FQMAVNFILSQTSPGAKALVIASDISRFLIAEGGDALSEDWSYAEPSAGAGAVAVLVGEN
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEECCC
PEVFQIDPGANGYYGYEVMDTCRPIPDSEAGDSDLSLMSYLDCCEQTFLEYQKRVPGANY
CCEEEECCCCCCEECHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
QDTFQYLAYHTPFGGMVKGAHRTMMRKVAKVKTSGIETDFLTRVKPGLNYCQRVGNIMGA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALFLALASTIDQGRFDTPKRIGCFSYGSGCCSEFYSGITTPQGQERQRTFGIEKHLDRRY
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHCHHH
QLSMEEYELLFKGSGMVRFGTRNVKLDFEMIPGIMQSTQEKPRLFLEEISEFHRKYRWIS
HCCHHHHHHHEECCCEEEECCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSAGIEAMNVFGGTAYLDVMELAKYRHLDTARFENLLMKEKAVALPYEDPVTFGVNAAK
CCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHH
PIIDALSEAEKDRIELLITCSESGIDFGKSLSTYIHEYLGLNRNCRLFEVKQACYSGTAG
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCH
FQMAVNFILSQTSPGAKALVIASDISRFLIAEGGDALSEDWSYAEPSAGAGAVAVLVGEN
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEECCC
PEVFQIDPGANGYYGYEVMDTCRPIPDSEAGDSDLSLMSYLDCCEQTFLEYQKRVPGANY
CCEEEECCCCCCEECHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
QDTFQYLAYHTPFGGMVKGAHRTMMRKVAKVKTSGIETDFLTRVKPGLNYCQRVGNIMGA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALFLALASTIDQGRFDTPKRIGCFSYGSGCCSEFYSGITTPQGQERQRTFGIEKHLDRRY
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHCHHH
QLSMEEYELLFKGSGMVRFGTRNVKLDFEMIPGIMQSTQEKPRLFLEEISEFHRKYRWIS
HCCHHHHHHHEECCCEEEECCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377