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The map label for this gene is pksH

Identifier: 16078777

GI number: 16078777

Start: 1791193

End: 1791972

Strand: Direct

Name: pksH

Synonym: BSU17160

Alternate gene names: 16078777

Gene position: 1791193-1791972 (Clockwise)

Preceding gene: 50812233

Following gene: 255767395

Centisome position: 42.49

GC content: 45.13

Gene sequence:

>780_bases
GTGGATCTCGTGACCTATCAAACGATAAAGGTCCGCTTTCAAGCATCGGTTTGCTATATTACCTTTCACCGTCCTGAAGC
GAATAATACGATTAATGACACGCTGATAGAAGAATGCTTGCAAGTGTTAAACCAATGTGAAACATCAACGGTGACGGTTG
TCGTTTTAGAGGGGCTTCCCGAGGTGTTTTGTTTCGGAGCGGATTTTCAAGAAATCTATCAGGAAATGAAAAGGGGCAGA
AAACAGGCAAGCTCCCAAGAGCCTCTCTATGATTTGTGGATGAAATTGCAGACCGGCCCTTATGTCACGATTTCGCATGT
CAGGGGAAAAGTGAATGCCGGCGGTCTTGGATTTGTATCTGCCACAGATATTGCCATTGCTGATCAGACGGCGTCATTCA
GTCTCTCTGAGCTGCTATTCGGCCTGTACCCTGCTTGTGTTTTACCGTTTCTGATCCGCCGTATCGGCCGGCAGAAAGCG
CATTATATGACGCTTATGACAAAGCCGATTTCCGTTCAGGAAGCCAGTGAATGGGGGTTAATAGATGCTTTTGATGCTGA
AAGTGACGTGCTGCTGAGAAAGCATTTATTGCGTTTGCGGAGGCTGAATAAAAAAGGAATCGCACATTATAAACAGTTTA
TGAGCTCACTTGATCATCAAGTCAGTCGTGCGAAAGCCACCGCTTTAACTGCAAATCAAGACATGTTTTCTGATCCTCAA
AACCAAATGGGAATCATCAGGTATGTTGAAACAGGACAATTCCCATGGGAGGATCAGTAA

Upstream 100 bases:

>100_bases
TAAAGCTGGACTTTGAGATGATACCGGGCATCATGCAGTCCACTCAAGAGAAGCCGCGCTTGTTTTTAGAAGAAATTTCT
GAGTTTCATCGGAAGTACAG

Downstream 100 bases:

>100_bases
CCGTTTAAAAATGACAATCAGAACCGTTAGGAGGACATCATGACGCATTCTGTTGTAGAGCTTATCGAAATTGAATCCGC
CATTATTCAGGTAAAGATGC

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDLVTYQTIKVRFQASVCYITFHRPEANNTINDTLIEECLQVLNQCETSTVTVVVLEGLPEVFCFGADFQEIYQEMKRGR
KQASSQEPLYDLWMKLQTGPYVTISHVRGKVNAGGLGFVSATDIAIADQTASFSLSELLFGLYPACVLPFLIRRIGRQKA
HYMTLMTKPISVQEASEWGLIDAFDAESDVLLRKHLLRLRRLNKKGIAHYKQFMSSLDHQVSRAKATALTANQDMFSDPQ
NQMGIIRYVETGQFPWEDQ

Sequences:

>Translated_259_residues
MDLVTYQTIKVRFQASVCYITFHRPEANNTINDTLIEECLQVLNQCETSTVTVVVLEGLPEVFCFGADFQEIYQEMKRGR
KQASSQEPLYDLWMKLQTGPYVTISHVRGKVNAGGLGFVSATDIAIADQTASFSLSELLFGLYPACVLPFLIRRIGRQKA
HYMTLMTKPISVQEASEWGLIDAFDAESDVLLRKHLLRLRRLNKKGIAHYKQFMSSLDHQVSRAKATALTANQDMFSDPQ
NQMGIIRYVETGQFPWEDQ
>Mature_259_residues
MDLVTYQTIKVRFQASVCYITFHRPEANNTINDTLIEECLQVLNQCETSTVTVVVLEGLPEVFCFGADFQEIYQEMKRGR
KQASSQEPLYDLWMKLQTGPYVTISHVRGKVNAGGLGFVSATDIAIADQTASFSLSELLFGLYPACVLPFLIRRIGRQKA
HYMTLMTKPISVQEASEWGLIDAFDAESDVLLRKHLLRLRRLNKKGIAHYKQFMSSLDHQVSRAKATALTANQDMFSDPQ
NQMGIIRYVETGQFPWEDQ

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably catalyzes the dehydration of the (S)-3-hydroxy-3-methylglutaryl group attached to pksL

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Escherichia coli, GI1787659, Length=180, Percent_Identity=29.4444444444444, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787660, Length=181, Percent_Identity=27.6243093922652, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PKSH_BACSU (P40805)

Other databases:

- EMBL:   U11039
- EMBL:   AL009126
- PIR:   F69678
- RefSeq:   NP_389596.1
- PDB:   3HP0
- PDBsum:   3HP0
- ProteinModelPortal:   P40805
- SMR:   P40805
- EnsemblBacteria:   EBBACT00000002837
- GeneID:   940033
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17160
- NMPDR:   fig|224308.1.peg.1718
- GenoList:   BSU17160
- GeneTree:   EBGT00070000032614
- HOGENOM:   HBG338003
- OMA:   GFQKAHY
- ProtClustDB:   PRK07112
- BioCyc:   BSUB:BSU17160-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 29411; Mature: 29411

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLVTYQTIKVRFQASVCYITFHRPEANNTINDTLIEECLQVLNQCETSTVTVVVLEGLP
CCCEEEEEEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
EVFCFGADFQEIYQEMKRGRKQASSQEPLYDLWMKLQTGPYVTISHVRGKVNAGGLGFVS
HHEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEE
ATDIAIADQTASFSLSELLFGLYPACVLPFLIRRIGRQKAHYMTLMTKPISVQEASEWGL
ECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEECCCCHHCCCCCCC
IDAFDAESDVLLRKHLLRLRRLNKKGIAHYKQFMSSLDHQVSRAKATALTANQDMFSDPQ
EEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCC
NQMGIIRYVETGQFPWEDQ
CCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MDLVTYQTIKVRFQASVCYITFHRPEANNTINDTLIEECLQVLNQCETSTVTVVVLEGLP
CCCEEEEEEEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
EVFCFGADFQEIYQEMKRGRKQASSQEPLYDLWMKLQTGPYVTISHVRGKVNAGGLGFVS
HHEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEE
ATDIAIADQTASFSLSELLFGLYPACVLPFLIRRIGRQKAHYMTLMTKPISVQEASEWGL
ECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEECCCCHHCCCCCCC
IDAFDAESDVLLRKHLLRLRRLNKKGIAHYKQFMSSLDHQVSRAKATALTANQDMFSDPQ
EEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCC
NQMGIIRYVETGQFPWEDQ
CCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377