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The map label for this gene is ptsI

Identifier: 50812224

GI number: 50812224

Start: 1459650

End: 1461362

Strand: Direct

Name: ptsI

Synonym: BSU13910

Alternate gene names: 50812224

Gene position: 1459650-1461362 (Clockwise)

Preceding gene: 16078454

Following gene: 16078456

Centisome position: 34.62

GC content: 43.84

Gene sequence:

>1713_bases
ATGCAAGAATTAAAAGGGATTGGTGCTTCAGCCGGCATCGCGATTGCAAAAGCGTACCGGCTTGAAGAGCCAGATTTAAC
GGTTGAAAAGAAAAACATCTCTGATTCTGAAGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGC
TCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTC
AGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAACGCTGAATTCGCTTTAAAAGAAAC
GTCTTCTATGTTCGTTACTATGTTTGAATCTATGGACAACGAATATATGAAAGAGCGTGCGGCAGATATCCGCGATGTAA
CAAAACGCGTAACCGGCCACCTGCTTGGCGTCGAAATTCCAAACCCAAGCATGATCTCAGAAGAGGTTATTATTGTAGCT
GAAGACTTAACACCGTCTGACACAGCTCAATTAAACCGTGAGTTCGTAAAAGGATTCACTACTGATATCGGCGGACGCAC
ATCTCACTCTGCCATCATGGCACGTTCTCTTGAGATTCCAGCAGTTGTTGGAACGAAAGCTGCTACAGGCACGATCCAAA
ACGGCGTGACTGTTATCGTTGACGGTATCAATGGCGATGTCATTATTGACCCATCTGCGGAAACTGTAAAAGAGTATGAA
GAAAAACATAATGCTTATTTGGCTCAAAAAGCTGAGTGGGCGAAGCTTGTCAATGAACCGACTGTCTCAAAAGACGGACA
TCATGTAGAGCTTGCAGCGAACATCGGTACTCCTGATGATGTAAAAGGTGTTCTTGAAAACGGCGGAGAAGCAGTTGGCC
TTTACCGTACAGAATTCCTTTACATGGGAAGAGATCAGCTGCCTACAGAAGATGAGCAGTTTGATGCTTACAAAACGGTT
CTTGAGCGTATGGAAGGAAAATCAGTCGTCGTGCGGACACTTGATATCGGCGGAGACAAAGAGCTTCCTTACCTGCAGCT
TCCTAAAGAAATGAACCCGTTCCTGGGATATAGAGCGATTCGTCTTTGCCTTGAAGAGCAAGAAATCTTCAGAACACAGC
TTCGCGCGCTGCTTCGTGCAAGTACATACGGAAACTTGAAAATCATGTTCCCTATGATTGCGACAGTTAACGAATTCAAA
GAAGCAAAAGCGATCCTTCTTGAAGAGAAAGAAAAGCTCGTAAAAGCGGGACAGGCTGTATCTGACGATATTGAAGTCGG
AATGATGGTTGAGATTCCGTCAACTGCAGTCATCGCTGATCAGTTTGCTAAAGAGGTTGATTTCTTCAGTATCGGAACAA
ACGATTTGATTCAATACACAATGGCAGCTGACCGTATGAATGAACGTGTATCTTACCTGTATCAGCCATACAACCCGGCA
ATCCTTCGCTTAATTACACTGGTAATTGAAGCAGCACACAAAGAAGGAAAATGGGTTGGCATGTGCGGAGAAATGGCAGG
AGACGAGATTGCGATTCCGATCCTTCTCGGCTTAGGCTTAGATGAGTTCTCAATGAGCGCAACGTCTATCCTTCCGGCAA
GAACACAAATCAGCAAATTGTCTAAACAAGAAGCTGAGTCATTCAAAGAGAAAATCTTATCTATGAGCACGACAGAAGAA
GTTGTCGCGTTCGTAAAAGAAACATTCAAGTAA

Upstream 100 bases:

>100_bases
CTAAAGGCGCTGAGATCACAATCTCTGCTTCCGGAGCTGACGAAAACGATGCTCTTAACGCTTTAGAAGAAACAATGAAA
AGCGAAGGACTCGGCGAGTA

Downstream 100 bases:

>100_bases
TGTACAAAAACCAGACGGCCTCCGGCCTGTCTGGTTTTTTTCATAAGTAAGGGTATAGAAGGACACAATAACATGGCTAG
GAGGGATAGTATGTCAAACC

Product: phosphotransferase system (PTS) enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MQELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEHALKELGQDKADIFSAHLLVL
SDPELLNPVKEKISTDSVNAEFALKETSSMFVTMFESMDNEYMKERAADIRDVTKRVTGHLLGVEIPNPSMISEEVIIVA
EDLTPSDTAQLNREFVKGFTTDIGGRTSHSAIMARSLEIPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYE
EKHNAYLAQKAEWAKLVNEPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEDEQFDAYKTV
LERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFK
EAKAILLEEKEKLVKAGQAVSDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKQEAESFKEKILSMSTTEE
VVAFVKETFK

Sequences:

>Translated_570_residues
MQELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEHALKELGQDKADIFSAHLLVL
SDPELLNPVKEKISTDSVNAEFALKETSSMFVTMFESMDNEYMKERAADIRDVTKRVTGHLLGVEIPNPSMISEEVIIVA
EDLTPSDTAQLNREFVKGFTTDIGGRTSHSAIMARSLEIPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYE
EKHNAYLAQKAEWAKLVNEPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEDEQFDAYKTV
LERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFK
EAKAILLEEKEKLVKAGQAVSDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKQEAESFKEKILSMSTTEE
VVAFVKETFK
>Mature_570_residues
MQELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEHALKELGQDKADIFSAHLLVL
SDPELLNPVKEKISTDSVNAEFALKETSSMFVTMFESMDNEYMKERAADIRDVTKRVTGHLLGVEIPNPSMISEEVIIVA
EDLTPSDTAQLNREFVKGFTTDIGGRTSHSAIMARSLEIPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYE
EKHNAYLAQKAEWAKLVNEPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEDEQFDAYKTV
LERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAIRLCLEEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFK
EAKAILLEEKEKLVKAGQAVSDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKQEAESFKEKILSMSTTEE
VVAFVKETFK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=49.469964664311, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI48994992, Length=491, Percent_Identity=37.8818737270876, Blast_Score=351, Evalue=8e-98,
Organism=Escherichia coli, GI1788726, Length=564, Percent_Identity=35.1063829787234, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789193, Length=572, Percent_Identity=32.6923076923077, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1787994, Length=429, Percent_Identity=28.9044289044289, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI226510935, Length=207, Percent_Identity=29.951690821256, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_BACSU (P08838)

Other databases:

- EMBL:   M98359
- EMBL:   AL009126
- EMBL:   X12832
- EMBL:   L08809
- PIR:   C46238
- RefSeq:   NP_389274.2
- ProteinModelPortal:   P08838
- SMR:   P08838
- PhosSite:   P08838
- EnsemblBacteria:   EBBACT00000003054
- GeneID:   939253
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13910
- GenoList:   BSU13910
- GeneTree:   EBGT00050000002837
- HOGENOM:   HBG456539
- OMA:   MVEIPST
- PhylomeDB:   P08838
- ProtClustDB:   CLSK928129
- BioCyc:   BSUB:BSU13910-MONOMER
- BRENDA:   2.7.3.9
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 63080; Mature: 63080

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 189-189 ACT_SITE 502-502 BINDING 296-296 BINDING 332-332 BINDING 431-431 BINDING 452-452 BINDING 453-453 BINDING 454-454 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEH
CCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
ALKELGQDKADIFSAHLLVLSDPELLNPVKEKISTDSVNAEFALKETSSMFVTMFESMDN
HHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
EYMKERAADIRDVTKRVTGHLLGVEIPNPSMISEEVIIVAEDLTPSDTAQLNREFVKGFT
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TDIGGRTSHSAIMARSLEIPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYE
HCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHH
EKHNAYLAQKAEWAKLVNEPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEEE
YMGRDQLPTEDEQFDAYKTVLERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAI
EECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHHH
RLCLEEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFKEAKAILLEEKEKLVKAGQAV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
CCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKL
HHHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEHHEECCCCHHHCCHHHHCCHHHHHHHH
SKQEAESFKEKILSMSTTEEVVAFVKETFK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSEAEVSRFDEAIARSKEELEKIKEH
CCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
ALKELGQDKADIFSAHLLVLSDPELLNPVKEKISTDSVNAEFALKETSSMFVTMFESMDN
HHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
EYMKERAADIRDVTKRVTGHLLGVEIPNPSMISEEVIIVAEDLTPSDTAQLNREFVKGFT
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TDIGGRTSHSAIMARSLEIPAVVGTKAATGTIQNGVTVIVDGINGDVIIDPSAETVKEYE
HCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHH
EKHNAYLAQKAEWAKLVNEPTVSKDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEEE
YMGRDQLPTEDEQFDAYKTVLERMEGKSVVVRTLDIGGDKELPYLQLPKEMNPFLGYRAI
EECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHHH
RLCLEEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFKEAKAILLEEKEKLVKAGQAV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
CCCCCEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKL
HHHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEHHEECCCCHHHCCHHHHCCHHHHHHHH
SKQEAESFKEKILSMSTTEEVVAFVKETFK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7686067; 9384377; 10568751; 2497294; 8449881; 1495386