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The map label for this gene is asnO

Identifier: 50812210

GI number: 50812210

Start: 1157237

End: 1159081

Strand: Direct

Name: asnO

Synonym: BSU10790

Alternate gene names: 50812210

Gene position: 1157237-1159081 (Clockwise)

Preceding gene: 16078141

Following gene: 16078144

Centisome position: 27.45

GC content: 47.53

Gene sequence:

>1845_bases
ATGTGTGGAATTACGGGTTGGGTCGATTTTAAAAAGCAGCTCGTCCAGGAAAAACAAACGATGGACAGAATGACAGACAC
TCTTTCCAAACGAGGGCCTGATGATTCAAACGTTTGGGGGGAGCACCATGTTTTATTCGGACATAAAAGACTTGCGGTTG
TTGATATTGAAGGCGGACGCCAGCCGATGGCGTGTACCTATAAAGGCGATACGTACACCATTATTTACAATGGCGAGCTT
TATAACACGGAAGATTTGCGCAAAGAATTGCGGGCGCGGGGCCATCAATTCGAGCGTACGTCAGATACCGAGGTGCTTCT
GCACAGCTACATCGAGTGGCAGGAAGACTGTGTCGACCATCTCAATGGCATATTCGCTTTCGCTGTGTGGGATGAAAAGC
GTAATCTGTTGTTTGCCGCGAGAGACCGTCTCGGTGTAAAGCCGTTTTTTTACACGAAGGAAGGCTCTTCATTTCTCTTC
GGGTCGGAAATCAAAGCAATCCTCGCGCACCCTGATATAAAAGCGCGAGTGGACAGAACGGGACTTTCTGAAATTTTCGG
TCTTGGACCGTCCAGAACCCCGGGCACTGGCATATTTAAAGGCATAAAAGAAATCCGCCCGGCTCACGCGCTGACATTTT
CCAAAGACGGACTTAATATTTGGCGTTATTGGAATGTTGAAAGCGAAAAACATACGGACAGTTTTGATGACACGGTTGCC
AATGTCAGATCGTTATTTCAGGACGCGGTGACGCGGCAGCTTGTGTCGGATGTGCCTGTTTGCACATTCCTATCAGGCGG
TCTGGACTCAAGCGCGATTACAGCGATTGCGGCGGGCCATTTTGAAAAGGAAGGAAAAGCACCGCTTCATACTTATTCGA
TTGATTACGAAGAGAACGATAAATATTTTCAAGCAAGCGCTTTTCAGCCGAATGATGACGGACCATGGATTGAAAAAATG
ACAGAGGCTTTCGGCACAACGCACCACAAATGCGTCATCAGCCAAAAAGACCTCGTTGATCATTTGGAGGAAGCTGTACT
GGTCAAAGACTTGCCGGGAATGGCAGATGTCGACTCATCCCTGCTGTGGTTTTGCCGGGAAATCAAAAAGGACTTTGTCG
TCAGCCTATCGGGAGAATGCGCGGATGAGATTTTCGGCGGGTACCCGTGGTTCCATACGGCAGATGTTGAGTCTGGCTTT
CCATGGATGAGATCAACGGAGGAGCGGATCAAGCTGCTTTCTGACTCATGGCAGAAAAAATTGAATCTGAAAGAATACGT
AAATGCCAAATATGAAGAAACCTTGGCAGAAACGCCATTATTAGACGGAGAAACGGGTGTGGATAAAGCAAGAAGACAGC
TGTTCTATTTAAATATGCTTTGGTTTATGACGAACCTTTTGGATCGGAAGGATCGCATGAGCATGGGGGCGAGCCTGGAA
GTGCGGGTGCCGTTCGCCGATCACCGGCTTGTGGAATATGTTTGGAATATTCCGTGGGAAATGAAAATGCATGATAATCG
GGAAAAAGGCATTTTGCGCAAAGCGCTTGAGGGCATTTTGCCTGATGACATTTTGTATCGCAAAAAAAGCCCATATCCGA
AAACACATCATCCGGAATATACAAAAGGCGTCAGCGAGTGGCTGAAAACGATCAGAAGCCAAAAGGACTCCGTGCTCCAT
ACCTTACTGGACAGAAAACAATTAGATCAGCTTTTAGAAACGGAAGGCTCCTCCTTCAAGGTCCCGTGGTTCGGGCAGCT
CATGAAGGGGCCTCAGCTGATCGCCCATCTTGCTCAAATTCATACATGGTTTGAAGCGTATCGCATTGATATTGACGAGA
GGTAG

Upstream 100 bases:

>100_bases
TATGGACAAATCAACTATCTGCCTATAGATGCATAAACTTACTAAGGTGCTGAAAGAAACGAAATTGTTACGAAAGAAAC
GGGATTGGAGCTGATGTCAC

Downstream 100 bases:

>100_bases
GTTCAATACAAATGTGATTAAAAGCCAGAGATGTATAGATTTAAATAAGAAGGATAGATGAGCAGGGAAATATATGCTCT
ATCTATCCTTTTTTGTACAA

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MCGITGWVDFKKQLVQEKQTMDRMTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEGGRQPMACTYKGDTYTIIYNGEL
YNTEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVDHLNGIFAFAVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLF
GSEIKAILAHPDIKARVDRTGLSEIFGLGPSRTPGTGIFKGIKEIRPAHALTFSKDGLNIWRYWNVESEKHTDSFDDTVA
NVRSLFQDAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEENDKYFQASAFQPNDDGPWIEKM
TEAFGTTHHKCVISQKDLVDHLEEAVLVKDLPGMADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWFHTADVESGF
PWMRSTEERIKLLSDSWQKKLNLKEYVNAKYEETLAETPLLDGETGVDKARRQLFYLNMLWFMTNLLDRKDRMSMGASLE
VRVPFADHRLVEYVWNIPWEMKMHDNREKGILRKALEGILPDDILYRKKSPYPKTHHPEYTKGVSEWLKTIRSQKDSVLH
TLLDRKQLDQLLETEGSSFKVPWFGQLMKGPQLIAHLAQIHTWFEAYRIDIDER

Sequences:

>Translated_614_residues
MCGITGWVDFKKQLVQEKQTMDRMTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEGGRQPMACTYKGDTYTIIYNGEL
YNTEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVDHLNGIFAFAVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLF
GSEIKAILAHPDIKARVDRTGLSEIFGLGPSRTPGTGIFKGIKEIRPAHALTFSKDGLNIWRYWNVESEKHTDSFDDTVA
NVRSLFQDAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEENDKYFQASAFQPNDDGPWIEKM
TEAFGTTHHKCVISQKDLVDHLEEAVLVKDLPGMADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWFHTADVESGF
PWMRSTEERIKLLSDSWQKKLNLKEYVNAKYEETLAETPLLDGETGVDKARRQLFYLNMLWFMTNLLDRKDRMSMGASLE
VRVPFADHRLVEYVWNIPWEMKMHDNREKGILRKALEGILPDDILYRKKSPYPKTHHPEYTKGVSEWLKTIRSQKDSVLH
TLLDRKQLDQLLETEGSSFKVPWFGQLMKGPQLIAHLAQIHTWFEAYRIDIDER
>Mature_614_residues
MCGITGWVDFKKQLVQEKQTMDRMTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEGGRQPMACTYKGDTYTIIYNGEL
YNTEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVDHLNGIFAFAVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLF
GSEIKAILAHPDIKARVDRTGLSEIFGLGPSRTPGTGIFKGIKEIRPAHALTFSKDGLNIWRYWNVESEKHTDSFDDTVA
NVRSLFQDAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEENDKYFQASAFQPNDDGPWIEKM
TEAFGTTHHKCVISQKDLVDHLEEAVLVKDLPGMADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWFHTADVESGF
PWMRSTEERIKLLSDSWQKKLNLKEYVNAKYEETLAETPLLDGETGVDKARRQLFYLNMLWFMTNLLDRKDRMSMGASLE
VRVPFADHRLVEYVWNIPWEMKMHDNREKGILRKALEGILPDDILYRKKSPYPKTHHPEYTKGVSEWLKTIRSQKDSVLH
TLLDRKQLDQLLETEGSSFKVPWFGQLMKGPQLIAHLAQIHTWFEAYRIDIDER

Specific function: Asparagine synthetase involved in sporulation

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI168229248, Length=411, Percent_Identity=28.9537712895377, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI168229252, Length=411, Percent_Identity=28.9537712895377, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI168229250, Length=411, Percent_Identity=28.9537712895377, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI296010848, Length=384, Percent_Identity=28.90625, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI296010852, Length=313, Percent_Identity=27.4760383386581, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI296010850, Length=313, Percent_Identity=27.4760383386581, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786889, Length=608, Percent_Identity=26.8092105263158, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71993933, Length=579, Percent_Identity=26.0794473229706, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=579, Percent_Identity=26.0794473229706, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17560178, Length=408, Percent_Identity=27.4509803921569, Blast_Score=106, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321563, Length=608, Percent_Identity=25.3289473684211, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325403, Length=609, Percent_Identity=24.7947454844007, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45553209, Length=545, Percent_Identity=25.8715596330275, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNO_BACSU (O05272)

Other databases:

- EMBL:   Z93940
- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   F69837
- RefSeq:   NP_388960.2
- ProteinModelPortal:   O05272
- EnsemblBacteria:   EBBACT00000000654
- GeneID:   939783
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10790
- NMPDR:   fig|224308.1.peg.1079
- GenoList:   BSU10790
- GeneTree:   EBGT00050000000005
- HOGENOM:   HBG752912
- OMA:   YSIDYEE
- PhylomeDB:   O05272
- ProtClustDB:   CLSK865557
- BioCyc:   BSUB:BSU10790-MONOMER
- BRENDA:   6.3.5.4
- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR01536

Pfam domain/function: PF00733 Asn_synthase

EC number: =6.3.5.4

Molecular weight: Translated: 70715; Mature: 70715

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS51278 GATASE_TYPE_2

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVDFKKQLVQEKQTMDRMTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEGGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEEECCCC
QPMACTYKGDTYTIIYNGELYNTEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVDH
CCEEEEECCCEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDRT
HCCEEEEEEECCCCCEEEEEHHCCCCCCEEEECCCCCEEEHHHHHHHHCCCCHHHHHCCC
GLSEIFGLGPSRTPGTGIFKGIKEIRPAHALTFSKDGLNIWRYWNVESEKHTDSFDDTVA
CHHHHHCCCCCCCCCHHHHHHHHHHCCHHEEEEECCCCEEEEEECCCCCCCCCHHHHHHH
NVRSLFQDAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEEND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
KYFQASAFQPNDDGPWIEKMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLPGMADVDSS
CEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKKDFVVSLSGECADEIFGGYPWFHTADVESGFPWMRSTEERIKLLSDSWQKK
HHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
LNLKEYVNAKYEETLAETPLLDGETGVDKARRQLFYLNMLWFMTNLLDRKDRMSMGASLE
CCHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VRVPFADHRLVEYVWNIPWEMKMHDNREKGILRKALEGILPDDILYRKKSPYPKTHHPEY
EECCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHH
TKGVSEWLKTIRSQKDSVLHTLLDRKQLDQLLETEGSSFKVPWFGQLMKGPQLIAHLAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCHHHHHHHHHH
HTWFEAYRIDIDER
HHHHHHHEECCCCC
>Mature Secondary Structure
MCGITGWVDFKKQLVQEKQTMDRMTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEGGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEEECCCC
QPMACTYKGDTYTIIYNGELYNTEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVDH
CCEEEEECCCEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDRT
HCCEEEEEEECCCCCEEEEEHHCCCCCCEEEECCCCCEEEHHHHHHHHCCCCHHHHHCCC
GLSEIFGLGPSRTPGTGIFKGIKEIRPAHALTFSKDGLNIWRYWNVESEKHTDSFDDTVA
CHHHHHCCCCCCCCCHHHHHHHHHHCCHHEEEEECCCCEEEEEECCCCCCCCCHHHHHHH
NVRSLFQDAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEEND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
KYFQASAFQPNDDGPWIEKMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLPGMADVDSS
CEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKKDFVVSLSGECADEIFGGYPWFHTADVESGFPWMRSTEERIKLLSDSWQKK
HHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
LNLKEYVNAKYEETLAETPLLDGETGVDKARRQLFYLNMLWFMTNLLDRKDRMSMGASLE
CCHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VRVPFADHRLVEYVWNIPWEMKMHDNREKGILRKALEGILPDDILYRKKSPYPKTHHPEY
EECCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHH
TKGVSEWLKTIRSQKDSVLHTLLDRKQLDQLLETEGSSFKVPWFGQLMKGPQLIAHLAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCHHHHHHHHHH
HTWFEAYRIDIDER
HHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721