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The map label for this gene is pbuE

Identifier: 50812189

GI number: 50812189

Start: 625125

End: 626291

Strand: Reverse

Name: pbuE

Synonym: BSU05800

Alternate gene names: 50812189

Gene position: 626291-625125 (Counterclockwise)

Preceding gene: 255767153

Following gene: 16077644

Centisome position: 14.86

GC content: 46.27

Gene sequence:

>1167_bases
ATGAATTTCAAAGTTTTCCTGCTTGCAGCTTCTACGATTGCAGTCGGATTGGTTGAGTTAATTGTGGGGGGAATTCTTCC
TCAGATCGCAAATGATTTAGACATTTCCATTGTTTCAGCCGGACAGCTGATCAGTGTGTTTGCGCTGGGATATGCAGTAT
CTGGGCCGCTGCTTTTGGCATTGACTGCGAAGATTGAGCGGAAGCGCTTATATCTAATTGCTTTATTTGTTTTCTTCCTT
AGCAATCTTGTCGCCTATTTCAGCCCTAATTTCGCAACACTGATGGTATCTAGGGTATTGGCAGCAATGAGTACAGGGCT
GATTGTTGTGCTTTCTTTAACAATTGCCCCGAAAATTGTAGCGCCTGAATACAGAGCCCGCGCAATCGGAATTATTTTTA
TGGGATTCAGCTCTGCTATCGCATTAGGCGTGCCGCTCGGAATCTTAATCAGCGATTCCTTCGGGTGGCGGATTTTGTTC
CTTGGCATCGGCCTGTTGGCACTGATCTCCATGCTGATTATTTCAATCTTCTTTGAAAGAATACCGGCGGAGAAAATGAT
TCCTTTCCGGGAACAACTCAAGACGATCGGTAATCTGAAAATCGCGAGTTCACACCTCGTCACGATGTTTACATTAGCTG
GCCACTACACGCTTTACGCTTACTTTGCGCCGTTTTTAGAAGAGACTCTTCACCTGAGCTCTTTCTGGGTCAGCATTTGT
TATTTCCTTTTCGGGATATCTGCTGTATGCGGAGGCCCTTTCGGAGGAGCGCTATCTGACCGGCTTGGTTCCTTTAAGAG
CATTTTGCTGGTGACCGGTTCCTTTGCCATCATTATGTTCTTACTGCCGCTGTCTACATCGTCTATGATTTTCTTTTTGC
CTGTCATGGTGATTTGGGGTCTTCTGAGCTGGAGCCTCGCCCCGGCTCAGCAAAGCTATTTAATTGAAATTGCACCTGAT
TCGTCTGACATTCAGCAAAGCTTTAACACATCGGCCCTCCAAGTCGGAATTGCCCTTGGCTCAGCCATTGGCGGTGTTGT
TTTGGATCAGACGGGTACTGTGGTGTCAACGGCATGGTGCGGCGGATCGATTGTGATCATCGCTGTCCTGTTTGCTTTCA
TTTCTTTAACAAGACCTGTTCAAACAGCAAAAAAATCCTCCTTGTAA

Upstream 100 bases:

>100_bases
ACCAGGAACCGTAAAATCCTGATTACAAAATTTGTTTATGACATTTTTTGTAATCAGGATTTTTTTTATTTATCAAAACA
TTTAAGTAAAGGAGTTTGTT

Downstream 100 bases:

>100_bases
AAGGAGGATTTTTTATTATTTAGCTGAAAGCTCATCTTCTGTGACCCATTTGTGGTTTTTCACTTTTTCGCCGCTCGTTG
TCGATGTGTAGTCAACCATG

Product: hypoxanthine efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNFKVFLLAASTIAVGLVELIVGGILPQIANDLDISIVSAGQLISVFALGYAVSGPLLLALTAKIERKRLYLIALFVFFL
SNLVAYFSPNFATLMVSRVLAAMSTGLIVVLSLTIAPKIVAPEYRARAIGIIFMGFSSAIALGVPLGILISDSFGWRILF
LGIGLLALISMLIISIFFERIPAEKMIPFREQLKTIGNLKIASSHLVTMFTLAGHYTLYAYFAPFLEETLHLSSFWVSIC
YFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPLSTSSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPD
SSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWCGGSIVIIAVLFAFISLTRPVQTAKKSSL

Sequences:

>Translated_388_residues
MNFKVFLLAASTIAVGLVELIVGGILPQIANDLDISIVSAGQLISVFALGYAVSGPLLLALTAKIERKRLYLIALFVFFL
SNLVAYFSPNFATLMVSRVLAAMSTGLIVVLSLTIAPKIVAPEYRARAIGIIFMGFSSAIALGVPLGILISDSFGWRILF
LGIGLLALISMLIISIFFERIPAEKMIPFREQLKTIGNLKIASSHLVTMFTLAGHYTLYAYFAPFLEETLHLSSFWVSIC
YFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPLSTSSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPD
SSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWCGGSIVIIAVLFAFISLTRPVQTAKKSSL
>Mature_388_residues
MNFKVFLLAASTIAVGLVELIVGGILPQIANDLDISIVSAGQLISVFALGYAVSGPLLLALTAKIERKRLYLIALFVFFL
SNLVAYFSPNFATLMVSRVLAAMSTGLIVVLSLTIAPKIVAPEYRARAIGIIFMGFSSAIALGVPLGILISDSFGWRILF
LGIGLLALISMLIISIFFERIPAEKMIPFREQLKTIGNLKIASSHLVTMFTLAGHYTLYAYFAPFLEETLHLSSFWVSIC
YFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPLSTSSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPD
SSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWCGGSIVIIAVLFAFISLTRPVQTAKKSSL

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily

Homologues:

Organism=Escherichia coli, GI1787947, Length=336, Percent_Identity=29.1666666666667, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787808, Length=347, Percent_Identity=25.3602305475504, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786595, Length=377, Percent_Identity=26.525198938992, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI145693198, Length=345, Percent_Identity=24.9275362318841, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBUE_BACSU (Q797E3)

Other databases:

- EMBL:   D88802
- EMBL:   AL009126
- PIR:   H69784
- RefSeq:   NP_388461.2
- ProteinModelPortal:   Q797E3
- EnsemblBacteria:   EBBACT00000003526
- GeneID:   937996
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU05800
- GenoList:   BSU05800
- GeneTree:   EBGT00070000031875
- HOGENOM:   HBG732715
- OMA:   SAFSVNY
- PhylomeDB:   Q797E3
- ProtClustDB:   CLSK2523187
- BioCyc:   BSUB:BSU05800-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 41535; Mature: 41535

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc895644)-; HASH(0xd4ea1a0)-; HASH(0xcc91864)-; HASH(0xbc02b34)-; HASH(0xccae694)-; HASH(0xc5c3238)-; HASH(0xcfb3704)-; HASH(0xc625520)-; HASH(0xcf11f94)-; HASH(0xd472c44)-; HASH(0xb0c0454)-; HASH(0xb2c4f64)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKVFLLAASTIAVGLVELIVGGILPQIANDLDISIVSAGQLISVFALGYAVSGPLLLA
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCHHHHH
LTAKIERKRLYLIALFVFFLSNLVAYFSPNFATLMVSRVLAAMSTGLIVVLSLTIAPKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHEEHHHHHCCCCC
APEYRARAIGIIFMGFSSAIALGVPLGILISDSFGWRILFLGIGLLALISMLIISIFFER
CCCHHHHEEEEEEECHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IPAEKMIPFREQLKTIGNLKIASSHLVTMFTLAGHYTLYAYFAPFLEETLHLSSFWVSIC
CCHHHCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPLSTSSMIFFLPVMVIWG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLSWSLAPAQQSYLIEIAPDSSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWC
HHHCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCEEEEECC
GGSIVIIAVLFAFISLTRPVQTAKKSSL
CCHHHHHHHHHHHHHHHCHHHHHHHCCH
>Mature Secondary Structure
MNFKVFLLAASTIAVGLVELIVGGILPQIANDLDISIVSAGQLISVFALGYAVSGPLLLA
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCHHHHH
LTAKIERKRLYLIALFVFFLSNLVAYFSPNFATLMVSRVLAAMSTGLIVVLSLTIAPKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHEEHHHHHCCCCC
APEYRARAIGIIFMGFSSAIALGVPLGILISDSFGWRILFLGIGLLALISMLIISIFFER
CCCHHHHEEEEEEECHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IPAEKMIPFREQLKTIGNLKIASSHLVTMFTLAGHYTLYAYFAPFLEETLHLSSFWVSIC
CCHHHCCCHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPLSTSSMIFFLPVMVIWG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLSWSLAPAQQSYLIEIAPDSSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWC
HHHCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCEEEEECC
GGSIVIIAVLFAFISLTRPVQTAKKSSL
CCHHHHHHHHHHHHHHHCHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377