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The map label for this gene is yxjA

Identifier: 255767823

GI number: 255767823

Start: 4005752

End: 4006945

Strand: Direct

Name: yxjA

Synonym: BSU39020

Alternate gene names: 255767823

Gene position: 4005752-4006945 (Clockwise)

Preceding gene: 255767820

Following gene: 255767826

Centisome position: 95.02

GC content: 44.72

Gene sequence:

>1194_bases
ATGTACTTTTTACTAAACCTTGTCGGTCTCATTGTGATTATGGCAGTTGTATTCCTATGTTCTCCGCAGAAAAAGAAAAT
CAAGTGGCGCCCGATCATTACGTTAATTGTTCTGGAATTGCTGATTACTTGGTTTATGCTGGGAACAAAAGTCGGAAGCT
GGGCCATCGGTAAAATCGGTGATTTCTTCACTTGGCTGATTGCTTGCGCCAGTGACGGTATCGCGTTTGCCTTTCCGTCA
GTGATGGCGAATGAAACAGTAGACTTTTTCTTTAGTGCGCTTCTTCCAATTATCTTTATCGTCACATTCTTTGATATTTT
AACGTATTTCGGCATTTTGCCTTGGCTGATTGATAAAATCGGATGGGTGATTTCAAAGGCTTCCCGCTTGCCGAAATTAG
AAAGCTTTTTCTCTATTCAAATGATGTTCCTTGGAAATACCGAAGCACTTGCGGTCATCCGCCAGCAGCTTACGGTTTTA
AGCAACAACCGCCTGCTCACATTCGGTTTAATGAGCATGAGCAGCATCAGCGGCTCCATTATCGGATCTTATCTTTCAAT
GGTGCCTGCGACATACGTGTTTACAGCCATTCCTTTGAACTGCTTAAACGCGCTGATTATCGCAAACCTGCTGAATCCTG
TTCACGTGCCGGAGGATGAAGATATCATCTATACACCGCCTAAAGAAGAGAAGAAAGACTTTTTCTCTACGATTTCTAAC
AGTATGCTTGTCGGCATGAACATGGTTATCGTTATTTTGGCAATGGTGATCGGATATGTCGCATTAACGTCAGCAGTTAA
TGGCATTCTTGGCGTTTTCGTACATGGCCTGACCATCCAGACAATTTTTGCTTATCTGTTCAGTCCGTTCGCATTCCTGC
TTGGACTGCCAGTGCACGATGCGATGTATGTTGCTCAGCTGATGGGCATGAAATTAGCAACGAACGAGTTTGTTGCGATG
CTTGACTTGAAAAACAATCTCAAATCCCTTCCGCCTCACACCGTTGCGGTGGCGACGACATTCCTGACGTCCTTTGCAAA
CTTCAGTACTGTCGGCATGATTTACGGCACGTACAATTCGATCCTTGACGGAGAAAAGTCAACGGTCATCGGGAGAAACG
TGTGGAAACTGCTCGTCAGCGGAATTGCGGTATCTTTATTAAGTGCTGCGATTGTCGGCCTGTTTGTGTGGTAG

Upstream 100 bases:

>100_bases
TTCTGTTTGCTCTTATTTTTCACACTTTCTGCACTTCCAGAATTTGTGAAGGATAAGAGCTTTTTTTGTTTCCATAATAA
CCCTCATAGGAGTTGCTATC

Downstream 100 bases:

>100_bases
AAACATTGAGCCATATCCCTTTTCGGATATGGCTCTTTTCATTATTGATAAATAGAGGCTGGCACCTGCACATCCCGGAT
TCCCGCTGCATAAGGCCCCA

Product: purine nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MYFLLNLVGLIVIMAVVFLCSPQKKKIKWRPIITLIVLELLITWFMLGTKVGSWAIGKIGDFFTWLIACASDGIAFAFPS
VMANETVDFFFSALLPIIFIVTFFDILTYFGILPWLIDKIGWVISKASRLPKLESFFSIQMMFLGNTEALAVIRQQLTVL
SNNRLLTFGLMSMSSISGSIIGSYLSMVPATYVFTAIPLNCLNALIIANLLNPVHVPEDEDIIYTPPKEEKKDFFSTISN
SMLVGMNMVIVILAMVIGYVALTSAVNGILGVFVHGLTIQTIFAYLFSPFAFLLGLPVHDAMYVAQLMGMKLATNEFVAM
LDLKNNLKSLPPHTVAVATTFLTSFANFSTVGMIYGTYNSILDGEKSTVIGRNVWKLLVSGIAVSLLSAAIVGLFVW

Sequences:

>Translated_397_residues
MYFLLNLVGLIVIMAVVFLCSPQKKKIKWRPIITLIVLELLITWFMLGTKVGSWAIGKIGDFFTWLIACASDGIAFAFPS
VMANETVDFFFSALLPIIFIVTFFDILTYFGILPWLIDKIGWVISKASRLPKLESFFSIQMMFLGNTEALAVIRQQLTVL
SNNRLLTFGLMSMSSISGSIIGSYLSMVPATYVFTAIPLNCLNALIIANLLNPVHVPEDEDIIYTPPKEEKKDFFSTISN
SMLVGMNMVIVILAMVIGYVALTSAVNGILGVFVHGLTIQTIFAYLFSPFAFLLGLPVHDAMYVAQLMGMKLATNEFVAM
LDLKNNLKSLPPHTVAVATTFLTSFANFSTVGMIYGTYNSILDGEKSTVIGRNVWKLLVSGIAVSLLSAAIVGLFVW
>Mature_397_residues
MYFLLNLVGLIVIMAVVFLCSPQKKKIKWRPIITLIVLELLITWFMLGTKVGSWAIGKIGDFFTWLIACASDGIAFAFPS
VMANETVDFFFSALLPIIFIVTFFDILTYFGILPWLIDKIGWVISKASRLPKLESFFSIQMMFLGNTEALAVIRQQLTVL
SNNRLLTFGLMSMSSISGSIIGSYLSMVPATYVFTAIPLNCLNALIIANLLNPVHVPEDEDIIYTPPKEEKKDFFSTISN
SMLVGMNMVIVILAMVIGYVALTSAVNGILGVFVHGLTIQTIFAYLFSPFAFLLGLPVHDAMYVAQLMGMKLATNEFVAM
LDLKNNLKSLPPHTVAVATTFLTSFANFSTVGMIYGTYNSILDGEKSTVIGRNVWKLLVSGIAVSLLSAAIVGLFVW

Specific function: Involved in the uptake of the purine ribonucleosides inosine and guanosine

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family

Homologues:

Organism=Homo sapiens, GI42542381, Length=417, Percent_Identity=26.6187050359712, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI227116277, Length=422, Percent_Identity=25.5924170616114, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI11545853, Length=417, Percent_Identity=26.8585131894484, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1788737, Length=392, Percent_Identity=34.9489795918367, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1788485, Length=420, Percent_Identity=27.6190476190476, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788488, Length=419, Percent_Identity=28.4009546539379, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17560276, Length=396, Percent_Identity=26.7676767676768, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=395, Percent_Identity=23.7974683544304, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45552519, Length=407, Percent_Identity=24.8157248157248, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45552517, Length=407, Percent_Identity=24.8157248157248, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19921868, Length=407, Percent_Identity=24.8157248157248, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281360430, Length=413, Percent_Identity=23.4866828087167, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUPG_BACSU (P42312)

Other databases:

- EMBL:   D83026
- EMBL:   AL009126
- PIR:   G70078
- RefSeq:   NP_391781.2
- EnsemblBacteria:   EBBACT00000001047
- GeneID:   937472
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39020
- NMPDR:   fig|224308.1.peg.3907
- GenoList:   BSU39020
- GeneTree:   EBGT00070000031902
- HOGENOM:   HBG713614
- ProtClustDB:   CLSK884785
- BioCyc:   BSUB:BSU39020-MONOMER
- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668
- PANTHER:   PTHR10590
- TIGRFAMs:   TIGR00804

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N

EC number: NA

Molecular weight: Translated: 43720; Mature: 43720

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13468d10)-; HASH(0x134c6f20)-; HASH(0x12bd4e74)-; HASH(0x12e29f5c)-; HASH(0x12cba2c8)-; HASH(0x13519788)-; HASH(0x12aaab8c)-; HASH(0x134d1b48)-; HASH(0x12dd3f0c)-; HASH(0x1350446c)-; HASH(0x13263e80)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFLLNLVGLIVIMAVVFLCSPQKKKIKWRPIITLIVLELLITWFMLGTKVGSWAIGKIG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DFFTWLIACASDGIAFAFPSVMANETVDFFFSALLPIIFIVTFFDILTYFGILPWLIDKI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWVISKASRLPKLESFFSIQMMFLGNTEALAVIRQQLTVLSNNRLLTFGLMSMSSISGSI
HHHHHHHHCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
IGSYLSMVPATYVFTAIPLNCLNALIIANLLNPVHVPEDEDIIYTPPKEEKKDFFSTISN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
SMLVGMNMVIVILAMVIGYVALTSAVNGILGVFVHGLTIQTIFAYLFSPFAFLLGLPVHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AMYVAQLMGMKLATNEFVAMLDLKNNLKSLPPHTVAVATTFLTSFANFSTVGMIYGTYNS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHH
ILDGEKSTVIGRNVWKLLVSGIAVSLLSAAIVGLFVW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MYFLLNLVGLIVIMAVVFLCSPQKKKIKWRPIITLIVLELLITWFMLGTKVGSWAIGKIG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DFFTWLIACASDGIAFAFPSVMANETVDFFFSALLPIIFIVTFFDILTYFGILPWLIDKI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWVISKASRLPKLESFFSIQMMFLGNTEALAVIRQQLTVLSNNRLLTFGLMSMSSISGSI
HHHHHHHHCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
IGSYLSMVPATYVFTAIPLNCLNALIIANLLNPVHVPEDEDIIYTPPKEEKKDFFSTISN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
SMLVGMNMVIVILAMVIGYVALTSAVNGILGVFVHGLTIQTIFAYLFSPFAFLLGLPVHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AMYVAQLMGMKLATNEFVAMLDLKNNLKSLPPHTVAVATTFLTSFANFSTVGMIYGTYNS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHH
ILDGEKSTVIGRNVWKLLVSGIAVSLLSAAIVGLFVW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377