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The map label for this gene is pgsB

Identifier: 255767777

GI number: 255767777

Start: 3699446

End: 3700627

Strand: Reverse

Name: pgsB

Synonym: BSU35900

Alternate gene names: 255767777

Gene position: 3700627-3699446 (Counterclockwise)

Preceding gene: 16080652

Following gene: 16080642

Centisome position: 87.78

GC content: 43.06

Gene sequence:

>1182_bases
ATGTGGTTACTCATTATAGCCTGTGCTGTCATACTGGTCATCGGAATATTAGAAAAACGACGACATCAGAAAAACATTGA
TGCCCTCCCTGTTCGGGTGAATATTAACGGCATCCGCGGAAAATCGACTGTGACAAGGCTGACAACCGGAATATTAATAG
AAGCCGGTTACAAGACTGTTGGAAAAACAACAGGAACAGATGCAAGAATGATTTACTGGGACACACCGGAGGAAAAGCCG
ATTAAACGGAAACCTCAGGGGCCGAATATCGGAGAGCAAAAAGAAGTCATGAGAGAAACAGTAGAAAGAGGGGCTAACGC
GATTGTCAGTGAATGCATGGCTGTTAACCCAGATTATCAAATCATCTTTCAGGAAGAACTTCTGCAGGCCAATATCGGCG
TCATTGTGAATGTTTTGGAAGACCATATGGATGTCATGGGGCCGACGCTTGATGAAATTGCAGAAGCGTTTACTGCTACA
ATTCCTTATAATGGCCATCTTGTCATTACAGATAGTGAATATACCGAGTTCTTTAAACAAAAAGCAAAAGAACGAAACAC
AAAAGTCATCATTGCTGATAACTCAAAAATTACAGATGAGTATTTACGTAAATTTGAATACATGGTATTCCCTGATAACG
CTTCTCTGGCGCTGGGTGTGGCTCAAGCACTCGGCATTGACGAAGAAACAGCATTTAAGGGAATGCTGAATGCGCCGCCA
GATCCGGGAGCAATGAGAATTCTTCCGCTGATCAGTCCGAGCGAGCCTGGGCACTTTGTTAATGGGTTTGCCGCAAACGA
CGCTTCTTCTACTTTGAATATATGGAAACGTGTAAAAGAAATCGGTTACCCGACCGATGATCCGATCATCATCATGAACT
GCCGCGCAGACCGTGTCGATCGGACACAGCAATTCGCAAATGACGTATTGCCTTATATTGAAGCAAGTGAACTGATCTTA
ATCGGTGAAACAACAGAACCGATCGTAAAAGCCTACGAAGAAGGCAAAATTCCTGCAGACAAACTGCATGATCTAGAGTA
TAAGTCAACAGATGAAATTATGGAATTGTTAAAGAAAAGAATGCACAACCGTGTCATATATGGCGTCGGCAATATTCATG
GTGCCGCAGAGCCTTTAATTGAAAAAATCCACGAATACAAGGTAAAGCAGCTCGTAAGCTAG

Upstream 100 bases:

>100_bases
TAGGGAAGATTATGTTACATAATGCCGATTGAGAATTCATAGTGATTCTATATACTGATGAATGAATTTACAACAATATA
GAAGGAGATGTCGAAAAGCA

Downstream 100 bases:

>100_bases
GGGGAAATGCAGACATGTTCGGATCAGATTTATACATCGCACTAATTTTAGGTGTACTACTCAGTTTAATTTTTGCGGAA
AAAACAGGGATCGTGCCGGC

Product: capsular polyglutamate synthetase

Products: NA

Alternate protein names: Gamma-polyglutamic acid synthase

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MWLLIIACAVILVIGILEKRRHQKNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTVGKTTGTDARMIYWDTPEEKP
IKRKPQGPNIGEQKEVMRETVERGANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMDVMGPTLDEIAEAFTAT
IPYNGHLVITDSEYTEFFKQKAKERNTKVIIADNSKITDEYLRKFEYMVFPDNASLALGVAQALGIDEETAFKGMLNAPP
DPGAMRILPLISPSEPGHFVNGFAANDASSTLNIWKRVKEIGYPTDDPIIIMNCRADRVDRTQQFANDVLPYIEASELIL
IGETTEPIVKAYEEGKIPADKLHDLEYKSTDEIMELLKKRMHNRVIYGVGNIHGAAEPLIEKIHEYKVKQLVS

Sequences:

>Translated_393_residues
MWLLIIACAVILVIGILEKRRHQKNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTVGKTTGTDARMIYWDTPEEKP
IKRKPQGPNIGEQKEVMRETVERGANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMDVMGPTLDEIAEAFTAT
IPYNGHLVITDSEYTEFFKQKAKERNTKVIIADNSKITDEYLRKFEYMVFPDNASLALGVAQALGIDEETAFKGMLNAPP
DPGAMRILPLISPSEPGHFVNGFAANDASSTLNIWKRVKEIGYPTDDPIIIMNCRADRVDRTQQFANDVLPYIEASELIL
IGETTEPIVKAYEEGKIPADKLHDLEYKSTDEIMELLKKRMHNRVIYGVGNIHGAAEPLIEKIHEYKVKQLVS
>Mature_393_residues
MWLLIIACAVILVIGILEKRRHQKNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTVGKTTGTDARMIYWDTPEEKP
IKRKPQGPNIGEQKEVMRETVERGANAIVSECMAVNPDYQIIFQEELLQANIGVIVNVLEDHMDVMGPTLDEIAEAFTAT
IPYNGHLVITDSEYTEFFKQKAKERNTKVIIADNSKITDEYLRKFEYMVFPDNASLALGVAQALGIDEETAFKGMLNAPP
DPGAMRILPLISPSEPGHFVNGFAANDASSTLNIWKRVKEIGYPTDDPIIIMNCRADRVDRTQQFANDVLPYIEASELIL
IGETTEPIVKAYEEGKIPADKLHDLEYKSTDEIMELLKKRMHNRVIYGVGNIHGAAEPLIEKIHEYKVKQLVS

Specific function: Catalyzes the biosynthesis of PGA (gamma-polyglutamic acid) from L-glutamate. Both the 44-kDa and the 33-kDa forms are required for PGA synthesis

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CAPB_BACSU (P96736)

Other databases:

- EMBL:   Z92953
- EMBL:   Z92954
- EMBL:   AB016245
- EMBL:   AB039950
- EMBL:   AB046355
- EMBL:   AL009126
- PIR:   F70069
- RefSeq:   NP_391471.2
- ProteinModelPortal:   P96736
- EnsemblBacteria:   EBBACT00000002277
- GeneID:   936833
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35900
- NMPDR:   fig|224308.1.peg.3597
- GenoList:   BSU35900
- ProtClustDB:   CLSK986223
- BioCyc:   BSUB:BSU35900-MONOMER
- GO:   GO:0016020
- InterPro:   IPR008337
- InterPro:   IPR013221
- Gene3D:   G3DSA:3.40.1190.10
- PRINTS:   PR01758

Pfam domain/function: PF08245 Mur_ligase_M; SSF53623 Mur_ligase_cen

EC number: NA

Molecular weight: Translated: 44017; Mature: 44017

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLLIIACAVILVIGILEKRRHQKNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTV
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHEEECCCHHH
GKTTGTDARMIYWDTPEEKPIKRKPQGPNIGEQKEVMRETVERGANAIVSECMAVNPDYQ
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IIFQEELLQANIGVIVNVLEDHMDVMGPTLDEIAEAFTATIPYNGHLVITDSEYTEFFKQ
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCEEEEECCHHHHHHHH
KAKERNTKVIIADNSKITDEYLRKFEYMVFPDNASLALGVAQALGIDEETAFKGMLNAPP
HHHCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCC
DPGAMRILPLISPSEPGHFVNGFAANDASSTLNIWKRVKEIGYPTDDPIIIMNCRADRVD
CCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
RTQQFANDVLPYIEASELILIGETTEPIVKAYEEGKIPADKLHDLEYKSTDEIMELLKKR
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHH
MHNRVIYGVGNIHGAAEPLIEKIHEYKVKQLVS
HCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWLLIIACAVILVIGILEKRRHQKNIDALPVRVNINGIRGKSTVTRLTTGILIEAGYKTV
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHEEECCCHHH
GKTTGTDARMIYWDTPEEKPIKRKPQGPNIGEQKEVMRETVERGANAIVSECMAVNPDYQ
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IIFQEELLQANIGVIVNVLEDHMDVMGPTLDEIAEAFTATIPYNGHLVITDSEYTEFFKQ
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCEEEEECCHHHHHHHH
KAKERNTKVIIADNSKITDEYLRKFEYMVFPDNASLALGVAQALGIDEETAFKGMLNAPP
HHHCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCC
DPGAMRILPLISPSEPGHFVNGFAANDASSTLNIWKRVKEIGYPTDDPIIIMNCRADRVD
CCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
RTQQFANDVLPYIEASELILIGETTEPIVKAYEEGKIPADKLHDLEYKSTDEIMELLKKR
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHH
MHNRVIYGVGNIHGAAEPLIEKIHEYKVKQLVS
HCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 10486244; 11751809; 9384377