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The map label for this gene is yvfI

Identifier: 255767768

GI number: 255767768

Start: 3509831

End: 3510490

Strand: Reverse

Name: yvfI

Synonym: BSU34180

Alternate gene names: 255767768

Gene position: 3510490-3509831 (Counterclockwise)

Preceding gene: 16080473

Following gene: 16080470

Centisome position: 83.27

GC content: 49.24

Gene sequence:

>660_bases
ATGATCAAAAATGGCGAATTGAAGCCGGGGGATAAACTGGACTCTGTTCAGGCGCTTGCTGAGAGCTTTCAAGTCAGCCG
TTCAGCGGTTCGCGAAGCACTTTCTGCGCTAAAAGCGATGGGGCTTGTCGAAATGAAACAGGGAGAAGGCACGTATCTGA
AGGAATTTGAGCTCAATCAAATTTCTCAGCCGCTCTCAGCCGCCCTTCTGATGAAAAAAGAGGACGTCAAGCAGCTGCTC
GAGGTCAGAAAACTGCTTGAAATCGGCGTGGCTTCACTAGCGGCTGAAAAAAGGACAGAAGCAGATCTCGAAAGAATTCA
GGATGCACTAAAGGAAATGGGCAGCATTGAAGCGGACGGGGAGCTGGGAGAGAAAGCAGACTTTGCATTTCATCTTGCGC
TTGCGGACGCTTCTCAAAATGAACTTCTTAAACACTTGATGAATCACGTGTCATCATTGCTGCTGGAAACAATGAGGGAA
ACGAGGAAAATCTGGCTGTTTTCCAAGAAGACCTCCGTTCAGCGGCTGTATGAGGAGCACGAACGGATTTACAATGCTGT
GGCTGCCGGGAACGGTGCACAGGCGGAAGCCGCCATGCTGGCGCATTTGACGAATGTGGAAGATGTGCTTTCGGGATATT
TCGAGGAAAATGTGCAATAA

Upstream 100 bases:

>100_bases
GTAAACAGCCAGACCTACAAGTTACGGGGTGAATTGCATTGAAATATAAACAGATTAAAACAAAAAAATATATGAAGAAG
TAGCGGATGCCCTATTAGAT

Downstream 100 bases:

>100_bases
CCAACTCATTTCCCGGGCAATTCCGCCGGTTCCGAATGATACGAACAACTGAGACTGAGCCGCAAATGGTTCAGTCTTTT
TACATGGCAGCCAGAGGGCT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLL
EVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEADGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRE
TRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ

Sequences:

>Translated_219_residues
MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLL
EVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEADGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRE
TRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ
>Mature_219_residues
MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLL
EVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEADGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRE
TRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786303, Length=212, Percent_Identity=31.1320754716981, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790032, Length=224, Percent_Identity=29.4642857142857, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789353, Length=212, Percent_Identity=30.6603773584906, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994955, Length=203, Percent_Identity=31.0344827586207, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790780, Length=219, Percent_Identity=30.5936073059361, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI87082208, Length=221, Percent_Identity=28.5067873303167, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789621, Length=226, Percent_Identity=27.4336283185841, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LUTR_BACSU (O07007)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   B70038
- RefSeq:   NP_391298.2
- ProteinModelPortal:   O07007
- SMR:   O07007
- EnsemblBacteria:   EBBACT00000002921
- GeneID:   936332
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34180
- GenoList:   BSU34180
- GeneTree:   EBGT00050000000781
- HOGENOM:   HBG700007
- ProtClustDB:   CLSK2816789
- BioCyc:   BSUB:BSU34180-MONOMER
- GO:   GO:0005622
- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00035
- SMART:   SM00895
- SMART:   SM00345

Pfam domain/function: PF07729 FCD; PF00392 GntR

EC number: NA

Molecular weight: Translated: 24317; Mature: 24317

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ISQPLSAALLMKKEDVKQLLEVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLYEEH
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ISQPLSAALLMKKEDVKQLLEVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLYEEH
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377