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The map label for this gene is sigL

Identifier: 16080473

GI number: 16080473

Start: 3512498

End: 3513808

Strand: Reverse

Name: sigL

Synonym: BSU34200

Alternate gene names: 16080473

Gene position: 3513808-3512498 (Counterclockwise)

Preceding gene: 16080475

Following gene: 255767768

Centisome position: 83.35

GC content: 45.77

Gene sequence:

>1311_bases
ATGGATATGAAACTTCAGCAAGTACAAGTATTAAAGCCTCAACTGACACAGGAGCTCAGGCAGGCCATCACGCTGCTTGG
CTATCATTCGGCAGAACTCGCCGAGTACATTGATGAGCTCTCACTGGAAAACCCTCTTATTGAACGAAAGGAAACAGACA
CACCGCCACTATCTTACCATAAAACAAACAAAAACAGGATGAATGCACAGGAAGCGGGCCTTCAATTAAGTAATCCGCAA
AAAACATTGCAGGATGCGTTAAAACAGCAGTCACTCGATATGAATCTGACAAACACTGAAAAAAAGATTTTTAATTATCT
GATTCATTCGCTTGATTCAAACGGGTACTTGGAAGAAGATATAGAGGAGGCAGCACGGCGATTATCTGTATCGGCCAAGG
AAGCGGAGGCTGTTTTGGCAAAACTCCAGTCGTTAGAACCGGCTGGAATCGGCGCGAGATCTTTGCAGGAATGCATCCTT
CTTCAATTACAGCGGCTGCCGAACAGAAATGAACAAGCGGAAATGCTGGTTTCAGCTCATTTTGATGCTTTTGCGCAAAA
AAAATGGAAAACGCTTTCAGTAGAGACGGGAATTCCGCTTCATACCATTCAAGATATCTCAGATGATATCGCGGCTCTTC
ATCCTAGACCTGGCCTTCTGTTTGCCCGGCCAGAGCAAGATGTGTATATTGAGCCTGACATTTTTATCACCGTAAAAAAT
GGGCACATCGCAGCGGAACTGAATACCCGCTCGTTTCCCGAGATCGATCTGCATCCGCAGTACAGAACGCTTTTATCATC
CGGCTCCTGTCAGGATACTGTCTCCTATTTATCTGCGAAATATCAGGAGTGGCGCTGGCTGAGCCGCGCGCTGCGGCAGC
GAAAACAGACAATCACGAGAATTATCAATGAACTGATCACACGCCAAAAGGATTTCTTTCTGAAAGGGCGAAGCGCAATG
AAGCCGCTGACTCTCAGAGAGGTGGCAGACTGTCTAAGCCTTCATGAATCAACTGTGAGCCGGGCCATTAAAGGGAAAAC
AATACAAACGCCTTACGGGCTGTTTGAGATGAAACTCTTTTTCTCAGCAAAAGCCGAGGCTTCAGGTGATGGAGATGCGT
CGAATTATGCAGTCAAAACGCATCTGGAAAACCTGATCAATCAAGAGGACAAAACAAAACCGCTTTCAGACCAAAAGCTT
GTCGATTTGCTGTATGAACAACACGGCATCCAAATCTCGAGACGCACCGTGGCAAAATATCGCGATCAAATGAATATTCC
GTCATCGGCAGCGAGAAAGCGATATAAATAA

Upstream 100 bases:

>100_bases
GGACGGAAAAAAGTTCAGACATTTCTTTGGCAGCTCCTTTTTCATAAATGTATACGGTGTGATCTCAATCATGTACAATG
AAGAAAGGGGAGTGAGCAAG

Downstream 100 bases:

>100_bases
AATCCTCCCTAGACGGGAGGATTTTTTTAAGGAATCATCCAGCTGAATACGTGATGCTGCAAAAAGGTGATGATACAGAC
GAGCAGCAGCAAAAACAGAC

Product: RNA polymerase sigma-54 factor (sigma-L)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MDMKLQQVQVLKPQLTQELRQAITLLGYHSAELAEYIDELSLENPLIERKETDTPPLSYHKTNKNRMNAQEAGLQLSNPQ
KTLQDALKQQSLDMNLTNTEKKIFNYLIHSLDSNGYLEEDIEEAARRLSVSAKEAEAVLAKLQSLEPAGIGARSLQECIL
LQLQRLPNRNEQAEMLVSAHFDAFAQKKWKTLSVETGIPLHTIQDISDDIAALHPRPGLLFARPEQDVYIEPDIFITVKN
GHIAAELNTRSFPEIDLHPQYRTLLSSGSCQDTVSYLSAKYQEWRWLSRALRQRKQTITRIINELITRQKDFFLKGRSAM
KPLTLREVADCLSLHESTVSRAIKGKTIQTPYGLFEMKLFFSAKAEASGDGDASNYAVKTHLENLINQEDKTKPLSDQKL
VDLLYEQHGIQISRRTVAKYRDQMNIPSSAARKRYK

Sequences:

>Translated_436_residues
MDMKLQQVQVLKPQLTQELRQAITLLGYHSAELAEYIDELSLENPLIERKETDTPPLSYHKTNKNRMNAQEAGLQLSNPQ
KTLQDALKQQSLDMNLTNTEKKIFNYLIHSLDSNGYLEEDIEEAARRLSVSAKEAEAVLAKLQSLEPAGIGARSLQECIL
LQLQRLPNRNEQAEMLVSAHFDAFAQKKWKTLSVETGIPLHTIQDISDDIAALHPRPGLLFARPEQDVYIEPDIFITVKN
GHIAAELNTRSFPEIDLHPQYRTLLSSGSCQDTVSYLSAKYQEWRWLSRALRQRKQTITRIINELITRQKDFFLKGRSAM
KPLTLREVADCLSLHESTVSRAIKGKTIQTPYGLFEMKLFFSAKAEASGDGDASNYAVKTHLENLINQEDKTKPLSDQKL
VDLLYEQHGIQISRRTVAKYRDQMNIPSSAARKRYK
>Mature_436_residues
MDMKLQQVQVLKPQLTQELRQAITLLGYHSAELAEYIDELSLENPLIERKETDTPPLSYHKTNKNRMNAQEAGLQLSNPQ
KTLQDALKQQSLDMNLTNTEKKIFNYLIHSLDSNGYLEEDIEEAARRLSVSAKEAEAVLAKLQSLEPAGIGARSLQECIL
LQLQRLPNRNEQAEMLVSAHFDAFAQKKWKTLSVETGIPLHTIQDISDDIAALHPRPGLLFARPEQDVYIEPDIFITVKN
GHIAAELNTRSFPEIDLHPQYRTLLSSGSCQDTVSYLSAKYQEWRWLSRALRQRKQTITRIINELITRQKDFFLKGRSAM
KPLTLREVADCLSLHESTVSRAIKGKTIQTPYGLFEMKLFFSAKAEASGDGDASNYAVKTHLENLINQEDKTKPLSDQKL
VDLLYEQHGIQISRRTVAKYRDQMNIPSSAARKRYK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family

Homologues:

Organism=Escherichia coli, GI1789594, Length=362, Percent_Identity=34.2541436464088, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): RP54_BACSU (P24219)

Other databases:

- EMBL:   M73443
- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   A41229
- RefSeq:   NP_391300.1
- ProteinModelPortal:   P24219
- SMR:   P24219
- EnsemblBacteria:   EBBACT00000000188
- GeneID:   936362
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34200
- NMPDR:   fig|224308.1.peg.3426
- GenoList:   BSU34200
- GeneTree:   EBGT00050000002191
- HOGENOM:   HBG613158
- OMA:   YKVTESI
- PhylomeDB:   P24219
- ProtClustDB:   CLSK887903
- BioCyc:   BSUB:BSU34200-MONOMER
- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634
- PRINTS:   PR00045
- TIGRFAMs:   TIGR02395

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD

EC number: NA

Molecular weight: Translated: 49701; Mature: 49701

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00717 SIGMA54_1; PS00718 SIGMA54_2; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMKLQQVQVLKPQLTQELRQAITLLGYHSAELAEYIDELSLENPLIERKETDTPPLSYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
KTNKNRMNAQEAGLQLSNPQKTLQDALKQQSLDMNLTNTEKKIFNYLIHSLDSNGYLEED
CCCCHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
IEEAARRLSVSAKEAEAVLAKLQSLEPAGIGARSLQECILLQLQRLPNRNEQAEMLVSAH
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FDAFAQKKWKTLSVETGIPLHTIQDISDDIAALHPRPGLLFARPEQDVYIEPDIFITVKN
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEEEC
GHIAAELNTRSFPEIDLHPQYRTLLSSGSCQDTVSYLSAKYQEWRWLSRALRQRKQTITR
CEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IINELITRQKDFFLKGRSAMKPLTLREVADCLSLHESTVSRAIKGKTIQTPYGLFEMKLF
HHHHHHHCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHH
FSAKAEASGDGDASNYAVKTHLENLINQEDKTKPLSDQKLVDLLYEQHGIQISRRTVAKY
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
RDQMNIPSSAARKRYK
HHHCCCCCHHHHCCCC
>Mature Secondary Structure
MDMKLQQVQVLKPQLTQELRQAITLLGYHSAELAEYIDELSLENPLIERKETDTPPLSYH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
KTNKNRMNAQEAGLQLSNPQKTLQDALKQQSLDMNLTNTEKKIFNYLIHSLDSNGYLEED
CCCCHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
IEEAARRLSVSAKEAEAVLAKLQSLEPAGIGARSLQECILLQLQRLPNRNEQAEMLVSAH
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FDAFAQKKWKTLSVETGIPLHTIQDISDDIAALHPRPGLLFARPEQDVYIEPDIFITVKN
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEEEC
GHIAAELNTRSFPEIDLHPQYRTLLSSGSCQDTVSYLSAKYQEWRWLSRALRQRKQTITR
CEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IINELITRQKDFFLKGRSAMKPLTLREVADCLSLHESTVSRAIKGKTIQTPYGLFEMKLF
HHHHHHHCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHH
FSAKAEASGDGDASNYAVKTHLENLINQEDKTKPLSDQKLVDLLYEQHGIQISRRTVAKY
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
RDQMNIPSSAARKRYK
HHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377