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The map label for this gene is copB

Identifier: 255767764

GI number: 255767764

Start: 3438853

End: 3440952

Strand: Reverse

Name: copB

Synonym: BSU33490

Alternate gene names: 255767764

Gene position: 3440952-3438853 (Counterclockwise)

Preceding gene: 255767765

Following gene: 16080401

Centisome position: 81.62

GC content: 47.29

Gene sequence:

>2100_bases
GTGAAACAGGAATATGTTCTGGACGGTTTGGATTGCAGCAATTGTGCCCGAAAAATCGAAAATGGAGTCAAAGGCATAAA
AGGCATTAACGGATGCGCGGTAAATTTTGCGGCAAGCACCTTAACTGTCTCAGCCGATGGGAAAGAAGAGCAATGGGTCA
CGAATAAAGTAGAGAAAAAAGTGAAATCAATTGATCCGCATGTAACGGTTCGCCAAAAGCATATAAAGAAATCAGCTGAT
GATGGCTATCGTAATCGAATGGTCAATATGCTGATCAGAATGGCGGCGGCTGTCATTCTCGGCGCAGCGGCATATTTGGT
TCAGTCGGGAACCATTGAATTTTTCCTTTTCCTCGGTGCTTATTTGATTATCGGCGGTGACATTATAATCCGCGCAGTCA
AAAACATCATCCGCGGTCAGGTGTTTGATGAGCATTTCTTAATGGCTCTTGCCACAATCGGCGCTTTTCTGATTCAGCAA
TACCCTGAGGGGGTCGCCGTTATGCTGTTTTATCAAATCGGTGAGCTTTTTCAAGGAGCCGCGGTCAGCCGTTCCAGAAA
ATCAATTAGTGCGTTAATGGACATACGGCCTGATTACGCCAATCTCAAAACAAAGAACGGCATCGAACAGGTTTCGTCGG
AGGACGTTCAGACAGGAGACATCATTGTGGTTAATCCGGGAGAAAGCATACCCCTGGACGGTAAGGTCGTGCAAGGGTCA
GCGATGGTTGATACATCTGCTTTAACAGGTGAGTCCGTTCCGAGAAAAGCTGCGGAAGGACAAGATGTAATGTCAGGCTT
TATCAATCAAAACGGCGTTCTGCATATTGAAGTCACAAAGGGCTATCAGGAATCAGCAGTATCAAAAATATTAGACTTGG
TTCAGAATGCGAGCAGCCGTAAAGCCCGGACAGAAAACTTTATCACGAAGTTTGCCAAGTATTATACACCTGCTGTGGTG
ATCATTGCGGTATTGCTGGCCTTTGTTCCGCCGCTTGTTCTTTCTGGCGCCGCGCTTTCCGATTGGGTGTACCGCGCGCT
TATCTTTTTAGTGATTTCTTGTCCTTGTGCGCTTGTTGTCTCTATTCCGCTCGGTTTCTTCGGTGGAATCGGAGCGGCCT
CAAAGGCCGGAGTGCTTGTAAAGGGAAGCAATTATTTAGAAGCTTTGAATCAAGTCAAATACGCGGTGTTTGATAAAACG
GGAACACTAACAAAAGGCAGCTTTGAAGTGACAGAAATCAAACCCGCGGAAGGTTTTACAAAGGATAGATTACTTGAAGC
GGCGGCATACGCTGAGCTTCATTCACAGCATCCGATTGCTGAATCTGTGCGTAAAGCTTACGGGAAAATGCTTTCCTCCG
ATGAGATTGAGTCATACGAAGAAATCTCCGGTCACGGCATTTTTGCAAAGGTAAATGGAACTGAGATCCTTGCTGGGAAT
AAAAAGCTCATGGAAAGAGAACAAATTGAGGATGTCCCGGATGAAAATGCTGGAACAATCGTACACGTTGCTGTAGATCA
ACGCTATGCCGGAGCAATCATCATAGCGGATGAGATAAAGGAAGACGCAGCACAGGCGGTTGCTGATCTGAAGTCGCTTG
GGATCAAACAGACAGCTATGCTGACGGGTGATTCGAAACAAACGGGAGAAGCCGTCGGAAAACAGCTTGGGATTGGTGAG
GTATATGCAGAGCTGCTGCCGCAAGATAAAGTCGCGCAGGTCGAAGCATTAGAAGCTAAGCTTTTGCCTAGTGAAAAACT
CATTTTTGTCGGTGACGGCATCAATGATACGCCGGTGCTTGCACGCGCAGATATAGGGGTTGCGATGGGAGGCCTTGGCT
CAGACGCCGCGGTAGAAGCCGCAGACATCGTGCTGATGACAGATCAGCCGTCAAAAATAGCAGAAGCGATTCGGATTGCG
AAACGGACACGGCGAATCGTGTGGCAAAACATCGGCTTTGCTTTAGGTGTGAAAGCGATCTTTCTCATTCTTGGTGCATT
TGGAATTGCGACCATGTGGGAAGCGGTTTTCTCAGATGTCGGCGTTACACTGCTTGCTGTGGCGAACGCCATGCGCGTCA
TGCGTCTCAAAAACAAATAA

Upstream 100 bases:

>100_bases
AACTTATATATGAGTATATGCTCATATATATAAAATAAATACAATACTCATTGATACGCTTTGAAGAGGGAACCCTAAAT
CGGAAGGTAAGGTGAGACTA

Downstream 100 bases:

>100_bases
ATTGTCGGAGAGAATTCATTTTTTCGGGGTTTTCTTATCAGCGCTTGTCTTGTTAAAATAAAGAAGTGGGTCAATTGCGA
TCCGCTTCTTTTTTAATTTT

Product: copper(I)-transporting ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MKQEYVLDGLDCSNCARKIENGVKGIKGINGCAVNFAASTLTVSADGKEEQWVTNKVEKKVKSIDPHVTVRQKHIKKSAD
DGYRNRMVNMLIRMAAAVILGAAAYLVQSGTIEFFLFLGAYLIIGGDIIIRAVKNIIRGQVFDEHFLMALATIGAFLIQQ
YPEGVAVMLFYQIGELFQGAAVSRSRKSISALMDIRPDYANLKTKNGIEQVSSEDVQTGDIIVVNPGESIPLDGKVVQGS
AMVDTSALTGESVPRKAAEGQDVMSGFINQNGVLHIEVTKGYQESAVSKILDLVQNASSRKARTENFITKFAKYYTPAVV
IIAVLLAFVPPLVLSGAALSDWVYRALIFLVISCPCALVVSIPLGFFGGIGAASKAGVLVKGSNYLEALNQVKYAVFDKT
GTLTKGSFEVTEIKPAEGFTKDRLLEAAAYAELHSQHPIAESVRKAYGKMLSSDEIESYEEISGHGIFAKVNGTEILAGN
KKLMEREQIEDVPDENAGTIVHVAVDQRYAGAIIIADEIKEDAAQAVADLKSLGIKQTAMLTGDSKQTGEAVGKQLGIGE
VYAELLPQDKVAQVEALEAKLLPSEKLIFVGDGINDTPVLARADIGVAMGGLGSDAAVEAADIVLMTDQPSKIAEAIRIA
KRTRRIVWQNIGFALGVKAIFLILGAFGIATMWEAVFSDVGVTLLAVANAMRVMRLKNK

Sequences:

>Translated_699_residues
MKQEYVLDGLDCSNCARKIENGVKGIKGINGCAVNFAASTLTVSADGKEEQWVTNKVEKKVKSIDPHVTVRQKHIKKSAD
DGYRNRMVNMLIRMAAAVILGAAAYLVQSGTIEFFLFLGAYLIIGGDIIIRAVKNIIRGQVFDEHFLMALATIGAFLIQQ
YPEGVAVMLFYQIGELFQGAAVSRSRKSISALMDIRPDYANLKTKNGIEQVSSEDVQTGDIIVVNPGESIPLDGKVVQGS
AMVDTSALTGESVPRKAAEGQDVMSGFINQNGVLHIEVTKGYQESAVSKILDLVQNASSRKARTENFITKFAKYYTPAVV
IIAVLLAFVPPLVLSGAALSDWVYRALIFLVISCPCALVVSIPLGFFGGIGAASKAGVLVKGSNYLEALNQVKYAVFDKT
GTLTKGSFEVTEIKPAEGFTKDRLLEAAAYAELHSQHPIAESVRKAYGKMLSSDEIESYEEISGHGIFAKVNGTEILAGN
KKLMEREQIEDVPDENAGTIVHVAVDQRYAGAIIIADEIKEDAAQAVADLKSLGIKQTAMLTGDSKQTGEAVGKQLGIGE
VYAELLPQDKVAQVEALEAKLLPSEKLIFVGDGINDTPVLARADIGVAMGGLGSDAAVEAADIVLMTDQPSKIAEAIRIA
KRTRRIVWQNIGFALGVKAIFLILGAFGIATMWEAVFSDVGVTLLAVANAMRVMRLKNK
>Mature_699_residues
MKQEYVLDGLDCSNCARKIENGVKGIKGINGCAVNFAASTLTVSADGKEEQWVTNKVEKKVKSIDPHVTVRQKHIKKSAD
DGYRNRMVNMLIRMAAAVILGAAAYLVQSGTIEFFLFLGAYLIIGGDIIIRAVKNIIRGQVFDEHFLMALATIGAFLIQQ
YPEGVAVMLFYQIGELFQGAAVSRSRKSISALMDIRPDYANLKTKNGIEQVSSEDVQTGDIIVVNPGESIPLDGKVVQGS
AMVDTSALTGESVPRKAAEGQDVMSGFINQNGVLHIEVTKGYQESAVSKILDLVQNASSRKARTENFITKFAKYYTPAVV
IIAVLLAFVPPLVLSGAALSDWVYRALIFLVISCPCALVVSIPLGFFGGIGAASKAGVLVKGSNYLEALNQVKYAVFDKT
GTLTKGSFEVTEIKPAEGFTKDRLLEAAAYAELHSQHPIAESVRKAYGKMLSSDEIESYEEISGHGIFAKVNGTEILAGN
KKLMEREQIEDVPDENAGTIVHVAVDQRYAGAIIIADEIKEDAAQAVADLKSLGIKQTAMLTGDSKQTGEAVGKQLGIGE
VYAELLPQDKVAQVEALEAKLLPSEKLIFVGDGINDTPVLARADIGVAMGGLGSDAAVEAADIVLMTDQPSKIAEAIRIA
KRTRRIVWQNIGFALGVKAIFLILGAFGIATMWEAVFSDVGVTLLAVANAMRVMRLKNK

Specific function: Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. Does not seem to transport copper [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=799, Percent_Identity=27.909887359199, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI115529486, Length=582, Percent_Identity=30.4123711340206, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI55743073, Length=558, Percent_Identity=31.8996415770609, Blast_Score=231, Evalue=2e-60,
Organism=Escherichia coli, GI1789879, Length=701, Percent_Identity=36.0912981455064, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1786691, Length=744, Percent_Identity=28.2258064516129, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1786914, Length=470, Percent_Identity=28.2978723404255, Blast_Score=146, Evalue=5e-36,
Organism=Escherichia coli, GI2367363, Length=574, Percent_Identity=24.5644599303136, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556548, Length=587, Percent_Identity=29.9829642248722, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI71997269, Length=174, Percent_Identity=30.4597701149425, Blast_Score=70, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71997275, Length=181, Percent_Identity=30.939226519337, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71997262, Length=181, Percent_Identity=30.939226519337, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71995298, Length=230, Percent_Identity=26.5217391304348, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71995291, Length=230, Percent_Identity=26.5217391304348, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71995286, Length=230, Percent_Identity=26.5217391304348, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17541776, Length=235, Percent_Identity=26.3829787234043, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6320475, Length=545, Percent_Identity=29.9082568807339, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6319772, Length=588, Percent_Identity=26.1904761904762, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321430, Length=560, Percent_Identity=23.3928571428571, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325221, Length=560, Percent_Identity=23.2142857142857, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321271, Length=191, Percent_Identity=30.8900523560209, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221329854, Length=393, Percent_Identity=29.0076335877863, Blast_Score=140, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24668708, Length=160, Percent_Identity=30.625, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24668704, Length=160, Percent_Identity=30.625, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281366617, Length=160, Percent_Identity=30.625, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3; =3.6.3.5 [H]

Molecular weight: Translated: 75038; Mature: 75038

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEYVLDGLDCSNCARKIENGVKGIKGINGCAVNFAASTLTVSADGKEEQWVTNKVEKK
CCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHH
VKSIDPHVTVRQKHIKKSADDGYRNRMVNMLIRMAAAVILGAAAYLVQSGTIEFFLFLGA
HHHCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YLIIGGDIIIRAVKNIIRGQVFDEHFLMALATIGAFLIQQYPEGVAVMLFYQIGELFQGA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCH
AVSRSRKSISALMDIRPDYANLKTKNGIEQVSSEDVQTGDIIVVNPGESIPLDGKVVQGS
HHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECC
AMVDTSALTGESVPRKAAEGQDVMSGFINQNGVLHIEVTKGYQESAVSKILDLVQNASSR
CEEECHHCCCCCCCCHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHH
KARTENFITKFAKYYTPAVVIIAVLLAFVPPLVLSGAALSDWVYRALIFLVISCPCALVV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SIPLGFFGGIGAASKAGVLVKGSNYLEALNQVKYAVFDKTGTLTKGSFEVTEIKPAEGFT
HHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCC
KDRLLEAAAYAELHSQHPIAESVRKAYGKMLSSDEIESYEEISGHGIFAKVNGTEILAGN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCEEEECC
KKLMEREQIEDVPDENAGTIVHVAVDQRYAGAIIIADEIKEDAAQAVADLKSLGIKQTAM
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEE
LTGDSKQTGEAVGKQLGIGEVYAELLPQDKVAQVEALEAKLLPSEKLIFVGDGINDTPVL
ECCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
ARADIGVAMGGLGSDAAVEAADIVLMTDQPSKIAEAIRIAKRTRRIVWQNIGFALGVKAI
EECCCCEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FLILGAFGIATMWEAVFSDVGVTLLAVANAMRVMRLKNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQEYVLDGLDCSNCARKIENGVKGIKGINGCAVNFAASTLTVSADGKEEQWVTNKVEKK
CCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHH
VKSIDPHVTVRQKHIKKSADDGYRNRMVNMLIRMAAAVILGAAAYLVQSGTIEFFLFLGA
HHHCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YLIIGGDIIIRAVKNIIRGQVFDEHFLMALATIGAFLIQQYPEGVAVMLFYQIGELFQGA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCH
AVSRSRKSISALMDIRPDYANLKTKNGIEQVSSEDVQTGDIIVVNPGESIPLDGKVVQGS
HHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECC
AMVDTSALTGESVPRKAAEGQDVMSGFINQNGVLHIEVTKGYQESAVSKILDLVQNASSR
CEEECHHCCCCCCCCHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHH
KARTENFITKFAKYYTPAVVIIAVLLAFVPPLVLSGAALSDWVYRALIFLVISCPCALVV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SIPLGFFGGIGAASKAGVLVKGSNYLEALNQVKYAVFDKTGTLTKGSFEVTEIKPAEGFT
HHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCC
KDRLLEAAAYAELHSQHPIAESVRKAYGKMLSSDEIESYEEISGHGIFAKVNGTEILAGN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCEEEECC
KKLMEREQIEDVPDENAGTIVHVAVDQRYAGAIIIADEIKEDAAQAVADLKSLGIKQTAM
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEE
LTGDSKQTGEAVGKQLGIGEVYAELLPQDKVAQVEALEAKLLPSEKLIFVGDGINDTPVL
ECCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
ARADIGVAMGGLGSDAAVEAADIVLMTDQPSKIAEAIRIAKRTRRIVWQNIGFALGVKAI
EECCCCEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FLILGAFGIATMWEAVFSDVGVTLLAVANAMRVMRLKNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]