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The map label for this gene is ytbJ

Identifier: 255767679

GI number: 255767679

Start: 3025748

End: 3026953

Strand: Reverse

Name: ytbJ

Synonym: BSU29580

Alternate gene names: 255767679

Gene position: 3026953-3025748 (Counterclockwise)

Preceding gene: 16080011

Following gene: 16080009

Centisome position: 71.8

GC content: 43.03

Gene sequence:

>1206_bases
ATGAATTACGATCATATATTAATTCGTTTTGGGGAAATTTCGACCAAAGGCAAAAACAGAAAAAGCTTTATTGAGCGCTT
AAAACAAAACATCAGACTCGTTTTGAAAGACTATCCGAATTTAAAATACTTTTCCAACAGGGATCGTATGACGATTACAT
TAAATGGAGAGGATCCTGAGGCTCTTTTTCCTCATTTAAAGCAAGTGTTTGGGATTCAAAGCTTCAGCCTGGCAATTAAG
TGTGACAGCCGCCTTGATGATATTAAGGCAACCGCACTAAAGGCTATCAAAGATCAGTATAAACCAGGTGATACGTTTAA
AGTCGCGACAAAGAGAGCGTATAAGCAATTTGAATTAGATACAAACCAGATGAACGCTGAAATCGGCGGCCATATTTTGC
GAAATACAGAAGGATTGACGGTCGATGTTCGCAACCCAGACATCCCGCTCAGAATCGAAATCAGGGAAGAAGCAACATTT
TTGACAATTCGTGATGAAAAAGGTGCAGGCGGGCTGCCAGTTGGTTCAGCAGGAAAAGCGATGCTGATGCTTTCTGGCGG
TTTTGACAGCCCTGTGGCCGGTTTTTATGCGATGAAACGGGGATTGTCGGTAGAAGCTGTTCATTTCTTCAGCCCGCCAT
ACACAAGCGAGCGCGCAAAGCAAAAGGTAATGGATCTGGCGAAATGTCTGTCCCGTTTTGGGGGAAGCATGACACTTCAT
ATTGTTCCATTTACAAAAACGCAAGAGCTCATCCAAAAACAAATTCCTGAAAATTACACAATGACGGCAACCCGCCGCCT
CATGCTTCAGATAGCCGACAGAATCAGAGAAAAGAGAAACGGGCTTGCCATTATTACGGGAGAAAGCCTCGGTCAGGTAG
CGAGCCAAACGCTTGAAAGCATGTATGCAATTAATGCGGTGACGTCAACACCGATTTTGCGTCCGCTAATCGCCATGGAC
AAAACAGAAATCATCGAAAAATCACGGGAAATTGGCACATATGAGACAAGTATTCAGCCGTTTGAAGACTGCTGCACGAT
CTTTACACCGCCAAGCCCGAAAACACGCCCTAAAAAAGAGAAAATTGAACACTTTGAAAGCTTTGTTGATTTCGAGCCGT
ATATTCAAGAAGCGGTAGATAACATTGAGACAATGACCCTTTATAGCGAACAAGAAGCTAACGATAAATTCGCTGAACTC
TTTTAA

Upstream 100 bases:

>100_bases
AATCAGTTTAAATTACAGCCAGACAAGCGATGTGGCAGAACCGTTTATGAACGCGCTTCGTCCTGGCATCAAAAAATTAA
GGGAAATGATGAGGTAGAAC

Downstream 100 bases:

>100_bases
ATCAATTTTCAGCTCCTGTATACAATTACCAAAGTTTTTCTGAATGAAGCCATGTGTTTTGACACATTCTATACTCACAA
GGAGGTGAGACACATGGCTA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNYDHILIRFGEISTKGKNRKSFIERLKQNIRLVLKDYPNLKYFSNRDRMTITLNGEDPEALFPHLKQVFGIQSFSLAIK
CDSRLDDIKATALKAIKDQYKPGDTFKVATKRAYKQFELDTNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATF
LTIRDEKGAGGLPVGSAGKAMLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSMTLH
IVPFTKTQELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLESMYAINAVTSTPILRPLIAMD
KTEIIEKSREIGTYETSIQPFEDCCTIFTPPSPKTRPKKEKIEHFESFVDFEPYIQEAVDNIETMTLYSEQEANDKFAEL
F

Sequences:

>Translated_401_residues
MNYDHILIRFGEISTKGKNRKSFIERLKQNIRLVLKDYPNLKYFSNRDRMTITLNGEDPEALFPHLKQVFGIQSFSLAIK
CDSRLDDIKATALKAIKDQYKPGDTFKVATKRAYKQFELDTNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATF
LTIRDEKGAGGLPVGSAGKAMLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSMTLH
IVPFTKTQELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLESMYAINAVTSTPILRPLIAMD
KTEIIEKSREIGTYETSIQPFEDCCTIFTPPSPKTRPKKEKIEHFESFVDFEPYIQEAVDNIETMTLYSEQEANDKFAEL
F
>Mature_401_residues
MNYDHILIRFGEISTKGKNRKSFIERLKQNIRLVLKDYPNLKYFSNRDRMTITLNGEDPEALFPHLKQVFGIQSFSLAIK
CDSRLDDIKATALKAIKDQYKPGDTFKVATKRAYKQFELDTNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATF
LTIRDEKGAGGLPVGSAGKAMLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSMTLH
IVPFTKTQELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLESMYAINAVTSTPILRPLIAMD
KTEIIEKSREIGTYETSIQPFEDCCTIFTPPSPKTRPKKEKIEHFESFVDFEPYIQEAVDNIETMTLYSEQEANDKFAEL
F

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=375, Percent_Identity=30.9333333333333, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_BACSU (O34595)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   E69988
- RefSeq:   NP_390836.2
- ProteinModelPortal:   O34595
- SMR:   O34595
- EnsemblBacteria:   EBBACT00000001537
- GeneID:   937471
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29580
- NMPDR:   fig|224308.1.peg.2961
- GenoList:   BSU29580
- GeneTree:   EBGT00050000001800
- HOGENOM:   HBG646072
- ProtClustDB:   PRK01565
- BioCyc:   BSUB:BSU29580-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45466; Mature: 45466

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDHILIRFGEISTKGKNRKSFIERLKQNIRLVLKDYPNLKYFSNRDRMTITLNGEDPE
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHEHHHCCCCCEEECCCCEEEEEECCCCHH
ALFPHLKQVFGIQSFSLAIKCDSRLDDIKATALKAIKDQYKPGDTFKVATKRAYKQFELD
HHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
TNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATFLTIRDEKGAGGLPVGSAGKA
CHHHHHHHCCEEEECCCCCEEEECCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCE
MLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSMTLH
EEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTKTQELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLES
EEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
MYAINAVTSTPILRPLIAMDKTEIIEKSREIGTYETSIQPFEDCCTIFTPPSPKTRPKKE
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCCCCHHH
KIEHFESFVDFEPYIQEAVDNIETMTLYSEQEANDKFAELF
HHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHCC
>Mature Secondary Structure
MNYDHILIRFGEISTKGKNRKSFIERLKQNIRLVLKDYPNLKYFSNRDRMTITLNGEDPE
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHEHHHCCCCCEEECCCCEEEEEECCCCHH
ALFPHLKQVFGIQSFSLAIKCDSRLDDIKATALKAIKDQYKPGDTFKVATKRAYKQFELD
HHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
TNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATFLTIRDEKGAGGLPVGSAGKA
CHHHHHHHCCEEEECCCCCEEEECCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCE
MLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSMTLH
EEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTKTQELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLES
EEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
MYAINAVTSTPILRPLIAMDKTEIIEKSREIGTYETSIQPFEDCCTIFTPPSPKTRPKKE
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCCCCHHH
KIEHFESFVDFEPYIQEAVDNIETMTLYSEQEANDKFAELF
HHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377