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The map label for this gene is iscS

Identifier: 16080011

GI number: 16080011

Start: 3026957

End: 3028102

Strand: Reverse

Name: iscS

Synonym: BSU29590

Alternate gene names: 16080011

Gene position: 3028102-3026957 (Counterclockwise)

Preceding gene: 16080013

Following gene: 255767679

Centisome position: 71.83

GC content: 44.68

Gene sequence:

>1146_bases
ATGATATATTTAGATAATAGTTCAACGACGAAGCCTTACGATGAAGTCTTGAACGTATATGAACAGACGAGCAGCCGATA
CTTTGGCAATCCTTCATCTTTGCACCGATATGGAGCCGAAACGGAACAGCTGCTTCAAGCAGCCAAAAATCAAATCAAAA
GGTCCTTAGGCCTAAAAAAATATGATATTGTATTTACGTCAGGCGCCACAGAAGCCAATAATGCGGCTTTAAAAGGAGCG
GCTTTGTCAAAGATAAAAACAGGCAAACATATCATTGCCACTTCTATTGAACATCCATCCGTTACGGAATCGTTAGAACA
GCTGAAAGAGTTGTTCGGGTTTGATATCACTTATCTTTCTGTGAATGAAGACGGATTTGTTTCTATTGAAGATCTAAAAG
CAGCCATCCGTCCCGATACCGTGCTTGTCAGCATGATGCATGTGAACAATGAAGTCGGTTCCGTGCAGCCGATTGAAGCG
GCAGGAGAAGTGCTGAAAGAGCATTCGAACATTTTGTTTCACGTCGATTATGTGCAGGGCATCTATAAAGTGCCCTTGGC
AATTGAAAAGGCCGGCATTGATCTATGTTCAATTTCGGGGCATAAATTTCACGGCCTAAAGGGGACAGGCGCACTTATTG
TAAAAGAAGGAACACGCCTTATTCCGCTCATAACGGGAGGCTCTCAGCAAAAAGGCATACGGGCCGGAACAGAACATACT
GCTGGTGCGGTTTCGCTTGCCAAGGCCATTAATCTGGCCTCTGCTGATTTTGACACACGGCTTGACACGATGACTGCCGT
AAAGGAATTGTTTATGAATAGGCTGAGTGAAATTGAGGGCGTCGTTATCAACACGCCTCAAATGAATAGCGCGCCGCACA
TTATTAATTTCTCTGTACCGGGGATTAAAGCGGAAGTGCTTTTACACATGCTTGAGGAACAGGATATATTCGTCTCCACA
ACATCGGCTTGCTCGGCAAAAGAACATAAGCCCAGCAAGGTTCTCTTAGAGATGGGCAAAGGAGAGCAGATTGCCGGAAG
CAGCATCAGAATCAGTTTAAATTACAGCCAGACAAGCGATGTGGCAGAACCGTTTATGAACGCGCTTCGTCCTGGCATCA
AAAAATTAAGGGAAATGATGAGGTAG

Upstream 100 bases:

>100_bases
AATTAGAAGTCAATACTAGTTTTTCGTAATTTTCTATTAAATTTTGTTATGGTAACATAAATATCATACCTTGTACCGCT
TTCATAAAGGAGTAAAAAAG

Downstream 100 bases:

>100_bases
AACATGAATTACGATCATATATTAATTCGTTTTGGGGAAATTTCGACCAAAGGCAAAAACAGAAAAAGCTTTATTGAGCG
CTTAAAACAAAACATCAGAC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKKYDIVFTSGATEANNAALKGA
ALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLSVNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEA
AGEVLKEHSNILFHVDYVQGIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEHT
AGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVST
TSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLREMMR

Sequences:

>Translated_381_residues
MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKKYDIVFTSGATEANNAALKGA
ALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLSVNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEA
AGEVLKEHSNILFHVDYVQGIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEHT
AGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVST
TSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLREMMR
>Mature_381_residues
MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKKYDIVFTSGATEANNAALKGA
ALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLSVNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEA
AGEVLKEHSNILFHVDYVQGIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEHT
AGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVST
TSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLREMMR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=382, Percent_Identity=36.9109947643979, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=29.5399515738499, Blast_Score=168, Evalue=8e-42,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=38.6422976501305, Blast_Score=250, Evalue=9e-68,
Organism=Escherichia coli, GI1787970, Length=274, Percent_Identity=23.3576642335766, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789175, Length=398, Percent_Identity=24.1206030150754, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=383, Percent_Identity=34.2036553524804, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=32.0987654320988, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=36.1256544502618, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI20129463, Length=382, Percent_Identity=35.6020942408377, Blast_Score=218, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS2_BACSU (O34874)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   F69666
- RefSeq:   NP_390837.1
- HSSP:   P0A6B7
- ProteinModelPortal:   O34874
- SMR:   O34874
- EnsemblBacteria:   EBBACT00000002758
- GeneID:   937701
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29590
- NMPDR:   fig|224308.1.peg.2962
- GenoList:   BSU29590
- GeneTree:   EBGT00050000000894
- HOGENOM:   HBG635316
- OMA:   MYVNNIM
- ProtClustDB:   CLSK887724
- BioCyc:   BSUB:BSU29590-MONOMER
- InterPro:   IPR000192
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 41488; Mature: 41488

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKK
CEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCEE
YDIVFTSGATEANNAALKGAALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLS
EEEEEECCCCCCCCCHHCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
VNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKEHSNILFHVDYVQG
ECCCCCEEHHHHHHHHCCCHHEEEHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHH
IYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEHT
HHHCCEEEECCCCCEEECCCCEECCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCC
AGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSVP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCC
GIKAEVLLHMLEEQDIFVSTTSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSD
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCCCCCEECCCEEEEEEECCCCHH
VAEPFMNALRPGIKKLREMMR
HHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKK
CEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCEE
YDIVFTSGATEANNAALKGAALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLS
EEEEEECCCCCCCCCHHCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
VNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKEHSNILFHVDYVQG
ECCCCCEEHHHHHHHHCCCHHEEEHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHH
IYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEHT
HHHCCEEEECCCCCEEECCCCEECCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCC
AGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSVP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCC
GIKAEVLLHMLEEQDIFVSTTSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQTSD
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCCCCCEECCCEEEEEEECCCCHH
VAEPFMNALRPGIKKLREMMR
HHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377