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The map label for this gene is yrkH

Identifier: 255767613

GI number: 255767613

Start: 2711109

End: 2712242

Strand: Reverse

Name: yrkH

Synonym: BSU26510

Alternate gene names: 255767613

Gene position: 2712242-2711109 (Counterclockwise)

Preceding gene: 16079706

Following gene: 16079703

Centisome position: 64.34

GC content: 39.86

Gene sequence:

>1134_bases
TTGAAACATGACCGTAAAGGCAATGACCCCAAAAGAAGTGACAAAAAAATCTTTAACAAAGAACCTTTATTTATTTTGGA
TGTGCGTAATGAAAATGATTTTAGTGATTGGAAGATAGAAGGGCATGACTTTGACCATTTGAATGTTCCTTATTTTGATC
TTTTAGATGGCGTAGAAGATATCTTGGAGAAAATTCCTTCTGATAAAGAAGTGCTGGTCGTTTGCGCAAAAGAAGGTTCA
TCCGTCATGGTATCCGAAATGCTTTCTGAAGCAGGTCATTCTGTATCCTACCTTAAGGGTGGAATGAAAGCATGGAGCGA
ACATTTAGAGCCGGTAAAAGTCGGGGATTTAAAAGATGGCGGTGAGATGTATCAGTTTGTTCGAATCGGTAAAGGCTGCT
TGTCATACATGGTCATTTCCAATGGTGAAGCAGCAATTATTGATGCGACACGTATGGTGAATTTCTTTATAGATTTTGCG
AAGGGGAAGGGTGCAACGATTACTCACGTATTTGATACACATCTTCACGCGGACCATATTTCAGGCGGCAGAGTCATTGC
TGAAAAAACAAAAGCAACCTACTGGTTACCGCCTAAAGATGCTGAAGAAGTGGAATTCGATTATCAGCCGTTGGAAGAAG
GAAATGACATTATTATTGGAAATACAACGATTAAAATTCAACCGATTTATTCTCCGGGGCACACGATTGGCTCCACTTCT
TTCATTGTCGATGACCAGTATCTTCTTTCAGGTGATATCTTGTTTATTGATTCAATCGGCAGACCAGACTTAGCCGGTAT
GGCAGAAGATTGGGTAGGAGATTTAAGAAAAACGCTTTATAGCCGTTACAAAGCGTTATCAAAGGATTTAATTGTATTAC
CGGCACACTTTATGATCATTGACGAGCTAAATGAGAATGGAAGTGTTGGTAAACAACTAAGTACATTATTTAGCGAGAAC
CATGGTTTGAACATTGCGGATGAAACAGAATTTAGACACCTAGTAACAGATCATTTGCCTCCGCAACCAAATGCATACCA
AGAGATTCGAGAAACAAACATGGGGAAAATCAGCCCAGATGAAGAAAAACAGCGTGAAATGGAGATTGGCCCAAACCGCT
GTGCAATACGGTAA

Upstream 100 bases:

>100_bases
GAATCGAAGTAGGCGGGGCTGTTACCTTTTTTGGTTTTACGAAAGATGCTGATGTATCCTTAACGTTTTAGTCCCGCTAA
AAGATTTGATATAGGAGGTA

Downstream 100 bases:

>100_bases
CACAAGTTTCTGCTAAAAAGAGGAGAGAAGAACATGAAATCAGATAAAGTATTAGACGCAAAAGGCTTGGCATGCCCAAT
GCCAATTGTCAGAACAAAAA

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKHDRKGNDPKRSDKKIFNKEPLFILDVRNENDFSDWKIEGHDFDHLNVPYFDLLDGVEDILEKIPSDKEVLVVCAKEGS
SVMVSEMLSEAGHSVSYLKGGMKAWSEHLEPVKVGDLKDGGEMYQFVRIGKGCLSYMVISNGEAAIIDATRMVNFFIDFA
KGKGATITHVFDTHLHADHISGGRVIAEKTKATYWLPPKDAEEVEFDYQPLEEGNDIIIGNTTIKIQPIYSPGHTIGSTS
FIVDDQYLLSGDILFIDSIGRPDLAGMAEDWVGDLRKTLYSRYKALSKDLIVLPAHFMIIDELNENGSVGKQLSTLFSEN
HGLNIADETEFRHLVTDHLPPQPNAYQEIRETNMGKISPDEEKQREMEIGPNRCAIR

Sequences:

>Translated_377_residues
MKHDRKGNDPKRSDKKIFNKEPLFILDVRNENDFSDWKIEGHDFDHLNVPYFDLLDGVEDILEKIPSDKEVLVVCAKEGS
SVMVSEMLSEAGHSVSYLKGGMKAWSEHLEPVKVGDLKDGGEMYQFVRIGKGCLSYMVISNGEAAIIDATRMVNFFIDFA
KGKGATITHVFDTHLHADHISGGRVIAEKTKATYWLPPKDAEEVEFDYQPLEEGNDIIIGNTTIKIQPIYSPGHTIGSTS
FIVDDQYLLSGDILFIDSIGRPDLAGMAEDWVGDLRKTLYSRYKALSKDLIVLPAHFMIIDELNENGSVGKQLSTLFSEN
HGLNIADETEFRHLVTDHLPPQPNAYQEIRETNMGKISPDEEKQREMEIGPNRCAIR
>Mature_377_residues
MKHDRKGNDPKRSDKKIFNKEPLFILDVRNENDFSDWKIEGHDFDHLNVPYFDLLDGVEDILEKIPSDKEVLVVCAKEGS
SVMVSEMLSEAGHSVSYLKGGMKAWSEHLEPVKVGDLKDGGEMYQFVRIGKGCLSYMVISNGEAAIIDATRMVNFFIDFA
KGKGATITHVFDTHLHADHISGGRVIAEKTKATYWLPPKDAEEVEFDYQPLEEGNDIIIGNTTIKIQPIYSPGHTIGSTS
FIVDDQYLLSGDILFIDSIGRPDLAGMAEDWVGDLRKTLYSRYKALSKDLIVLPAHFMIIDELNENGSVGKQLSTLFSEN
HGLNIADETEFRHLVTDHLPPQPNAYQEIRETNMGKISPDEEKQREMEIGPNRCAIR

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YRKH_BACSU (P54435)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   F69976
- RefSeq:   NP_390528.2
- ProteinModelPortal:   P54435
- EnsemblBacteria:   EBBACT00000004005
- GeneID:   937656
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU26510
- NMPDR:   fig|224308.1.peg.2653
- GenoList:   BSU26510
- GeneTree:   EBGT00050000000396
- HOGENOM:   HBG313796
- ProtClustDB:   CLSK2540371
- BioCyc:   BSUB:BSU26510-MONOMER
- InterPro:   IPR001279
- InterPro:   IPR001763
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00849
- SMART:   SM00450

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: NA

Molecular weight: Translated: 42400; Mature: 42400

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 75-75

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHDRKGNDPKRSDKKIFNKEPLFILDVRNENDFSDWKIEGHDFDHLNVPYFDLLDGVED
CCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
ILEKIPSDKEVLVVCAKEGSSVMVSEMLSEAGHSVSYLKGGMKAWSEHLEPVKVGDLKDG
HHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCC
GEMYQFVRIGKGCLSYMVISNGEAAIIDATRMVNFFIDFAKGKGATITHVFDTHLHADHI
HHHHHHHHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
SGGRVIAEKTKATYWLPPKDAEEVEFDYQPLEEGNDIIIGNTTIKIQPIYSPGHTIGSTS
CCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECEEEEEEEECCCCCCCCCEE
FIVDDQYLLSGDILFIDSIGRPDLAGMAEDWVGDLRKTLYSRYKALSKDLIVLPAHFMII
EEECCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
DELNENGSVGKQLSTLFSENHGLNIADETEFRHLVTDHLPPQPNAYQEIRETNMGKISPD
EECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
EEKQREMEIGPNRCAIR
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKHDRKGNDPKRSDKKIFNKEPLFILDVRNENDFSDWKIEGHDFDHLNVPYFDLLDGVED
CCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
ILEKIPSDKEVLVVCAKEGSSVMVSEMLSEAGHSVSYLKGGMKAWSEHLEPVKVGDLKDG
HHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCC
GEMYQFVRIGKGCLSYMVISNGEAAIIDATRMVNFFIDFAKGKGATITHVFDTHLHADHI
HHHHHHHHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
SGGRVIAEKTKATYWLPPKDAEEVEFDYQPLEEGNDIIIGNTTIKIQPIYSPGHTIGSTS
CCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECEEEEEEEECCCCCCCCCEE
FIVDDQYLLSGDILFIDSIGRPDLAGMAEDWVGDLRKTLYSRYKALSKDLIVLPAHFMII
EEECCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
DELNENGSVGKQLSTLFSENHGLNIADETEFRHLVTDHLPPQPNAYQEIRETNMGKISPD
EECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
EEKQREMEIGPNRCAIR
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377