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The map label for this gene is spoIVCA

Identifier: 255767592

GI number: 255767592

Start: 2653371

End: 2654873

Strand: Reverse

Name: spoIVCA

Synonym: BSU25770

Alternate gene names: 255767592

Gene position: 2654873-2653371 (Counterclockwise)

Preceding gene: 16079631

Following gene: 16079628

Centisome position: 62.98

GC content: 37.19

Gene sequence:

>1503_bases
GTGATAGCAATATATGTAAGGGTATCGACCGAGGAACAAGCGATCAAGGGATCGAGCATCGACAGCCAAATCGAGGCCTG
TATAAAGAAAGCAGGGACTAAAGATGTGCTGAAGTATGCAGATGAAGGATTTTCAGGAGAGCTTTTAGAACGTCCGGCTT
TGAATCGCTTGAGGGAGGATGCAAGCAAGGGACTTATAAGTCAAGTCATTTGTTACGATCCTGACCGTCTTTCTCGGAAA
TTAATGAATCAGCTAATCATTGATGACGAATTGCGAAAGCGAAACATACCTTTGATTTTTGTAAATGGTGAATACGCCAA
TTCTCCAGAAGGTCAATTGTTTTTCGCAATGCGCGGGGCAATCTCAGAATTTGAAAAAGCCAAAATCAAAGAACGGACAT
CAAGCGGCCGACTTCAAAAAATGAAAAAAGGCATGATCATTAAAGATTCTAAACTATATGGCTATAAATTTGTTAAAGAG
AAAAGAACTCTTGAGATATTAGAAGAGGAAGCAAAAATCATTCGGATGATTTTTAACTATTTCACCGATCATAAAAGCCC
TTTTTTCGGCAGAGTAAATGGTATTGCTCTACATTTAACTCAGATGGGGGTTAAAACAAAAAAAGGCGCCAAAGTATGGC
ACAGGCAGGTTGTTCGGCAAATATTAATGAACTCTTCCTATAAGGGTGAACATAGACAGTATAAATATGATACAGAGGGT
TCCTATGTTTCAAAGCAGGCAGGGAACAAATCTATAATTAAAATAAGGCCTGAAGAAGAACAAATCACTGTGACAATTCC
AGCAATTGTTCCAGCTGAACAATGGGATTATGCTCAAGAACTCTTAGGTCAAAGTAAAAGAAAACACTTGAGTATCAGCC
CTCACAATTACTTGTTATCGGGTTTGGTTAGATGCGGAAAATGCGGAAATACCATGACAGGGAAGAAAAGAAAATCACAT
GGTAAAGACTACTATGTATATACTTGCCGGAAAAATTATTCTGGCGCAAAGGACCGCGGCTGCGGAAAAGAAATGTCTGA
GAATAAATTGAACCGGCATGTATGGGGTGAAATTTTTAAATTCATCACAAATCCTCAAAAGTATGTTTCTTTTAAAGAGG
CTGAACAATCAAATCACCTGTCTGATGAATTAGAACTTATTGAAAAAGAGATAGAGAAAACAAAAAAAGGCCGCAAGCGT
CTTTTAACGCTAATCAGCCTAAGCGATGACGATGATTTAGACATAGATGAAATCAAAGCACAAATTATTGAACTGCAAAA
AAAGCAAAATCAGCTTACTGAAAAGTGTAACGAAATCCAGTCAAAAATGAAAGTCCTAGATGATACGAGCTCAAGTGAAA
ATGCTCTAAAAAGAGCCATCGACTATTTTCAATCAATCGGTGCAGATAACTTAACTCTTGAAGATAAAAAAACAATTGTT
AACTTTATCGTGAAAGAAGTTACCATTGTGGATTCTGACACCATATATATTGAAACGTATTAA

Upstream 100 bases:

>100_bases
CATTTTATGAAAGTGATAATCATCGAGGGACCGCAAGCTGACAAATGCATTAACGATTGCTATCATTATTTAATAAAACT
TTATAGGAAGGAGATTCAGG

Downstream 100 bases:

>100_bases
AGAGGGGTGTATGCACCCCCCTTTTGTAATTACAATCTCATTTTCAATACACCTCGCTGCATACGTCGCCAGCTTTGTCC
CTTTTCCAGCGGAATAGCTT

Product: site-specific DNA recombinase

Products: NA

Alternate protein names: Stage IV sporulation protein CA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MIAIYVRVSTEEQAIKGSSIDSQIEACIKKAGTKDVLKYADEGFSGELLERPALNRLREDASKGLISQVICYDPDRLSRK
LMNQLIIDDELRKRNIPLIFVNGEYANSPEGQLFFAMRGAISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKE
KRTLEILEEEAKIIRMIFNYFTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHRQYKYDTEG
SYVSKQAGNKSIIKIRPEEEQITVTIPAIVPAEQWDYAQELLGQSKRKHLSISPHNYLLSGLVRCGKCGNTMTGKKRKSH
GKDYYVYTCRKNYSGAKDRGCGKEMSENKLNRHVWGEIFKFITNPQKYVSFKEAEQSNHLSDELELIEKEIEKTKKGRKR
LLTLISLSDDDDLDIDEIKAQIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLTLEDKKTIV
NFIVKEVTIVDSDTIYIETY

Sequences:

>Translated_500_residues
MIAIYVRVSTEEQAIKGSSIDSQIEACIKKAGTKDVLKYADEGFSGELLERPALNRLREDASKGLISQVICYDPDRLSRK
LMNQLIIDDELRKRNIPLIFVNGEYANSPEGQLFFAMRGAISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKE
KRTLEILEEEAKIIRMIFNYFTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHRQYKYDTEG
SYVSKQAGNKSIIKIRPEEEQITVTIPAIVPAEQWDYAQELLGQSKRKHLSISPHNYLLSGLVRCGKCGNTMTGKKRKSH
GKDYYVYTCRKNYSGAKDRGCGKEMSENKLNRHVWGEIFKFITNPQKYVSFKEAEQSNHLSDELELIEKEIEKTKKGRKR
LLTLISLSDDDDLDIDEIKAQIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLTLEDKKTIV
NFIVKEVTIVDSDTIYIETY
>Mature_500_residues
MIAIYVRVSTEEQAIKGSSIDSQIEACIKKAGTKDVLKYADEGFSGELLERPALNRLREDASKGLISQVICYDPDRLSRK
LMNQLIIDDELRKRNIPLIFVNGEYANSPEGQLFFAMRGAISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKE
KRTLEILEEEAKIIRMIFNYFTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHRQYKYDTEG
SYVSKQAGNKSIIKIRPEEEQITVTIPAIVPAEQWDYAQELLGQSKRKHLSISPHNYLLSGLVRCGKCGNTMTGKKRKSH
GKDYYVYTCRKNYSGAKDRGCGKEMSENKLNRHVWGEIFKFITNPQKYVSFKEAEQSNHLSDELELIEKEIEKTKKGRKR
LLTLISLSDDDDLDIDEIKAQIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLTLEDKKTIV
NFIVKEVTIVDSDTIYIETY

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CISA_BACSU (P17867)

Other databases:

- EMBL:   M29040
- EMBL:   D32216
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A43656
- RefSeq:   NP_390454.2
- ProteinModelPortal:   P17867
- SMR:   P17867
- EnsemblBacteria:   EBBACT00000001252
- GeneID:   937799
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25770
- NMPDR:   fig|224308.1.peg.2579
- GenoList:   BSU25770
- GeneTree:   EBGT00050000001729
- HOGENOM:   HBG615335
- ProtClustDB:   CLSK806688
- BioCyc:   BSUB:BSU25770-MONOMER
- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119
- Gene3D:   G3DSA:3.40.50.1390
- SMART:   SM00857

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase; SSF53041 Resolv_N

EC number: NA

Molecular weight: Translated: 57456; Mature: 57456

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00397 RECOMBINASES_1; PS00398 RECOMBINASES_2

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIYVRVSTEEQAIKGSSIDSQIEACIKKAGTKDVLKYADEGFSGELLERPALNRLRED
CEEEEEEECCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCHHHHHHHHH
ASKGLISQVICYDPDRLSRKLMNQLIIDDELRKRNIPLIFVNGEYANSPEGQLFFAMRGA
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCCCCCEEEEHHHH
ISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKEKRTLEILEEEAKIIRMIFNY
HHHHHHHHHHHHHCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHRQYKYDTEG
HHCCCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
SYVSKQAGNKSIIKIRPEEEQITVTIPAIVPAEQWDYAQELLGQSKRKHLSISPHNYLLS
CEEHHCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHH
GLVRCGKCGNTMTGKKRKSHGKDYYVYTCRKNYSGAKDRGCGKEMSENKLNRHVWGEIFK
HHHHHCCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FITNPQKYVSFKEAEQSNHLSDELELIEKEIEKTKKGRKRLLTLISLSDDDDLDIDEIKA
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHH
QIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLTLEDKKTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
NFIVKEVTIVDSDTIYIETY
HHHHHHHEEEECCEEEEEEC
>Mature Secondary Structure
MIAIYVRVSTEEQAIKGSSIDSQIEACIKKAGTKDVLKYADEGFSGELLERPALNRLRED
CEEEEEEECCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCHHHHHHHHH
ASKGLISQVICYDPDRLSRKLMNQLIIDDELRKRNIPLIFVNGEYANSPEGQLFFAMRGA
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCCCCCEEEEHHHH
ISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKEKRTLEILEEEAKIIRMIFNY
HHHHHHHHHHHHHCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHRQYKYDTEG
HHCCCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
SYVSKQAGNKSIIKIRPEEEQITVTIPAIVPAEQWDYAQELLGQSKRKHLSISPHNYLLS
CEEHHCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHH
GLVRCGKCGNTMTGKKRKSHGKDYYVYTCRKNYSGAKDRGCGKEMSENKLNRHVWGEIFK
HHHHHCCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FITNPQKYVSFKEAEQSNHLSDELELIEKEIEKTKKGRKRLLTLISLSDDDDLDIDEIKA
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHH
QIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLTLEDKKTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
NFIVKEVTIVDSDTIYIETY
HHHHHHHEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377