Click here to switch to the map view.

The map label for this gene is nucB

Identifier: 16079628

GI number: 16079628

Start: 2652387

End: 2652797

Strand: Reverse

Name: nucB

Synonym: BSU25750

Alternate gene names: 16079628

Gene position: 2652797-2652387 (Counterclockwise)

Preceding gene: 255767592

Following gene: 16079626

Centisome position: 62.93

GC content: 51.09

Gene sequence:

>411_bases
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Upstream 100 bases:

>100_bases
GAGTCCGGCTTTGGTAAGCAGCATACTAACTTCAAATGTTAAAAATATTCTTCATCAAGCGCCCATACATTGAAATGAAC
AAAGGTATGGGGGGATGGGG

Downstream 100 bases:

>100_bases
TAAAGAAGAGGCTCTTTCTTGAGCCTCTGTTGTTACAATCCTTCGCCGAGCTGAATTTTCGGGGGATAGGTTTCTGCTTT
TCGCCAATATTGGCGTGTGC

Product: nuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Sequences:

>Translated_136_residues
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
>Mature_136_residues
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Specific function: Degrades both double-stranded linear and covalently closed circular DNA. Likely to play a scavenging role in order to supply nutrients under starvation conditions

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis NucA/ComI

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUCB_BACSU (P42983)

Other databases:

- EMBL:   S78249
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   M29040
- PIR:   S61273
- RefSeq:   NP_390452.1
- EnsemblBacteria:   EBBACT00000004081
- GeneID:   937804
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25750
- NMPDR:   fig|224308.1.peg.2577
- GenoList:   BSU25750
- GeneTree:   EBGT00050000001809
- HOGENOM:   HBG338008
- OMA:   KGIPTKP
- ProtClustDB:   CLSK887611
- BioCyc:   BSUB:BSU25750-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 14968; Mature: 14968

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDIC
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEE
TIDRDGADKRREESLKGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGN
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCC
QMSSYPDGTRVLFIVQ
HHHCCCCCCEEEEEEC
>Mature Secondary Structure
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDIC
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEE
TIDRDGADKRREESLKGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGN
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCC
QMSSYPDGTRVLFIVQ
HHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7746143; 8969508; 9384377; 2105293