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The map label for this gene is aroE

Identifier: 255767590

GI number: 255767590

Start: 2644630

End: 2645472

Strand: Reverse

Name: aroE

Synonym: BSU25660

Alternate gene names: 255767590

Gene position: 2645472-2644630 (Counterclockwise)

Preceding gene: 16079621

Following gene: 16079619

Centisome position: 62.75

GC content: 43.06

Gene sequence:

>843_bases
ATGAAAAAGCTGTACGGGGTTATCGGAAATCCTATTGGCCATTCTATGTCACCTGATATTCATAACGCATCATTGAAAGA
CCTCGGGCTTGACGGACATTATCATGCCTTCAAGGTAGAAGAGAATGATCTGGAGGATGCGGTAAAAGGAATAAGAGCTC
TAGGCGTACAAGGAATCAATGTGACTGTTCCGCACAAGGTTTCCATTATGGATTATTTGGATCATATTGATGAGAGTGCA
AAAGTGCTCGGTGCCGTAAACACGGTCAGAAGAGAGGGAGATAAGCTTGTCGGATACAATACCGATGGGGAAGGCTTCGT
GAAGTCATTAATGAAGGTATTAGACAAGCCCATCTCTGAACTGTCATTCTTAATGATCGGCGCTGGCGGAGCGGCGAGAG
CCATTTTTACAACAATTGTCCGAAATACCCCGAAAAAATTTGACATTTGCAACCGGACGTTAGAAAAAGCAAAGCGGCTT
ACAGAAGCTACTCCCTCGTTTCACAATAAAGAAGTATTAAGCATAAAAGAAGCAGAAGAACGTCTTGAGCAGTATGATGT
TATCATCCATACAACGTCTGTGGGCATGTATCCGAACGTGGACGATGTGCCGCTTTCATTACAGCGTGCCGCGAGCAGTG
CCGTCGTATGTGATATCGTGTATAATCCAATACAGACGGCACTTTTAAAAGAAGCAAGCCAAAAAGGCTTGAAAACACTC
GATGGTGTGGGCATGTTTGTTGAACAGGCAGCATTGTCTTTCCAATTGTGGACTGGACAAGAGCCTAATATTGAAAAAAT
GAGATCTATTGTAATAGGAAAATTAGGAGGAACAGAATGTTAA

Upstream 100 bases:

>100_bases
TTACTGTCCATGACGCTGATAAAAAGGTAACTGCATACGCGCCAAAAGGCGTTCATGTATTTGTTCGGCGTTCATTAATT
TAAGAAAAGGGGAGAGGTGT

Downstream 100 bases:

>100_bases
CAGGTAAACAAAAACGTTTTTTGCGTTCAAAAGCGCATCATTTAACGCCGATTTTTCAAGTAGGTAAAGGCGGAGTAAAC
GACAACATGATTAAACAAAT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGINVTVPHKVSIMDYLDHIDESA
KVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRL
TEATPSFHNKEVLSIKEAEERLEQYDVIIHTTSVGMYPNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTL
DGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC

Sequences:

>Translated_280_residues
MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGINVTVPHKVSIMDYLDHIDESA
KVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRL
TEATPSFHNKEVLSIKEAEERLEQYDVIIHTTSVGMYPNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTL
DGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC
>Mature_280_residues
MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGINVTVPHKVSIMDYLDHIDESA
KVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRL
TEATPSFHNKEVLSIKEAEERLEQYDVIIHTTSVGMYPNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTL
DGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=282, Percent_Identity=33.6879432624113, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789675, Length=267, Percent_Identity=34.8314606741573, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320332, Length=272, Percent_Identity=35.2941176470588, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_BACSU (P54374)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A69590
- RefSeq:   NP_390444.2
- ProteinModelPortal:   P54374
- SMR:   P54374
- EnsemblBacteria:   EBBACT00000001857
- GeneID:   937820
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25660
- NMPDR:   fig|224308.1.peg.2569
- GenoList:   BSU25660
- GeneTree:   EBGT00050000000826
- HOGENOM:   HBG553408
- PhylomeDB:   P54374
- ProtClustDB:   PRK00258
- BioCyc:   BSUB:BSU25660-MONOMER
- BRENDA:   1.1.1.25
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30609; Mature: 30609

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGIN
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCE
VTVPHKVSIMDYLDHIDESAKVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISE
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCHHHH
LSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRLTEATPSFHNKEVLSIKEAEE
HHEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RLEQYDVIIHTTSVGMYPNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTL
HHHHHEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
DGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC
HHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGIN
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCE
VTVPHKVSIMDYLDHIDESAKVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISE
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCHHHH
LSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRLTEATPSFHNKEVLSIKEAEE
HHEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RLEQYDVIIHTTSVGMYPNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTL
HHHHHEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
DGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC
HHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377