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The map label for this gene is spoIVB

Identifier: 255767535

GI number: 255767535

Start: 2519102

End: 2520382

Strand: Reverse

Name: spoIVB

Synonym: BSU24230

Alternate gene names: 255767535

Gene position: 2520382-2519102 (Counterclockwise)

Preceding gene: 255767536

Following gene: 16079478

Centisome position: 59.79

GC content: 40.75

Gene sequence:

>1281_bases
ATGCCCGATAACATCAGAAAAGCAGTAGGTTTAATTCTCCTTGTTTCGTTATTAAGTGTAGGTTTATGCAAACCGCTAAA
AGAATATTTACTGATTCCAACGCAAATGAGAGTATTTGAAACCCAAACACAAGCGATTGAAACGAGTTTATCGGTAAACG
CTCAGACATCAGAATCCTCAGAAGCGTTTACAGTAAAGAAAGATCCGCATGAAATCAAGGTGACGGGCAAAAAATCAGGT
GAGTCAGAATTGGTATATGATCTTGCCGGATTTCCAATTAAAAAAACAAAAGTGCATGTTCTTCCTGATTTAAAAGTTAT
ACCTGGCGGACAATCAATCGGTGTAAAACTTCATTCCGTCGGTGTTCTTGTCGTCGGATTTCATCAAATCAATACAAGTG
AAGGCAAAAAATCTCCGGGAGAAACGGCAGGAATTGAAGCGGGCGACATCATTATTGAGATGAATGGACAGAAAATTGAA
AAAATGAATGATGTAGCCCCATTTATTCAAAAGGCTGGGAAAACTGGTGAATCTTTAGACTTACTGATCAAACGTGATAA
ACAGAAAATCAAAACGAAGCTGATCCCAGAAAAGGATGAAGGAGAAGGCAAATACAGAATCGGGTTATATATCAGAGATT
CTGCTGCTGGCATCGGCACTATGACCTTTTATGAACCGAAAACAAAAAAATACGGAGCACTTGGCCACGTGATTTCCGAT
ATGGACACAAAGAAACCAATTGTAGTGGAGAATGGAGAAATCGTTAAATCCACTGTAACATCAATTGAAAAAGGGACAGG
CGGTAATCCGGGAGAAAAACTGGCGCGATTTTCCTCAGAACGCAAAACGATCGGGGATATTAACAGAAACAGCCCGTTTG
GGATTTTCGGCACACTGCATCAGCCGATTCAAAACAACATATCAGATCAAGCATTGCCGGTTGCGTTTTCTACCGAAGTC
AAAAAAGGGCCGGCTGAAATTTTAACGGTTATTGATGATGACAAAGTAGAAAAATTCGATATTGAAATCGTCAGCACAAC
GCCGCAAAAATTCCCTGCGACAAAAGGAATGGTGTTGAAAATTACCGATCCAAGACTGTTGAAAGAAACAGGAGGCATCG
TACAGGGGATGAGCGGAAGCCCGATCATTCAAAATGGAAAAGTGATCGGTGCTGTCACCCATGTATTTGTAAATGACCCG
ACAAGCGGCTACGGTGTTCATATTGAATGGATGCTGTCAGAAGCAGGAATCGATATTTATGGAAAAGAAAAAGCAAGCTG
A

Upstream 100 bases:

>100_bases
TATGACAGTTATAAATAAGCCGTCAGAAGGCAAAATTAAATGATGTAGCAGCAAGTCATAAAGAAGGTGTGGGATAGGAG
CGAGGAGAGTGAAGTAGTGA

Downstream 100 bases:

>100_bases
CTGCCGGAGTTTCCGGCAGTTTTTTTATTTTGATCCCTCTTCACTTCTCAGAATACATACGGTAAAATATACAAAAGAAG
ATTTTTCGACAAATTCACGT

Product: regulatory membrane-associated serine protease

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MPDNIRKAVGLILLVSLLSVGLCKPLKEYLLIPTQMRVFETQTQAIETSLSVNAQTSESSEAFTVKKDPHEIKVTGKKSG
ESELVYDLAGFPIKKTKVHVLPDLKVIPGGQSIGVKLHSVGVLVVGFHQINTSEGKKSPGETAGIEAGDIIIEMNGQKIE
KMNDVAPFIQKAGKTGESLDLLIKRDKQKIKTKLIPEKDEGEGKYRIGLYIRDSAAGIGTMTFYEPKTKKYGALGHVISD
MDTKKPIVVENGEIVKSTVTSIEKGTGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLHQPIQNNISDQALPVAFSTEV
KKGPAEILTVIDDDKVEKFDIEIVSTTPQKFPATKGMVLKITDPRLLKETGGIVQGMSGSPIIQNGKVIGAVTHVFVNDP
TSGYGVHIEWMLSEAGIDIYGKEKAS

Sequences:

>Translated_426_residues
MPDNIRKAVGLILLVSLLSVGLCKPLKEYLLIPTQMRVFETQTQAIETSLSVNAQTSESSEAFTVKKDPHEIKVTGKKSG
ESELVYDLAGFPIKKTKVHVLPDLKVIPGGQSIGVKLHSVGVLVVGFHQINTSEGKKSPGETAGIEAGDIIIEMNGQKIE
KMNDVAPFIQKAGKTGESLDLLIKRDKQKIKTKLIPEKDEGEGKYRIGLYIRDSAAGIGTMTFYEPKTKKYGALGHVISD
MDTKKPIVVENGEIVKSTVTSIEKGTGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLHQPIQNNISDQALPVAFSTEV
KKGPAEILTVIDDDKVEKFDIEIVSTTPQKFPATKGMVLKITDPRLLKETGGIVQGMSGSPIIQNGKVIGAVTHVFVNDP
TSGYGVHIEWMLSEAGIDIYGKEKAS
>Mature_425_residues
PDNIRKAVGLILLVSLLSVGLCKPLKEYLLIPTQMRVFETQTQAIETSLSVNAQTSESSEAFTVKKDPHEIKVTGKKSGE
SELVYDLAGFPIKKTKVHVLPDLKVIPGGQSIGVKLHSVGVLVVGFHQINTSEGKKSPGETAGIEAGDIIIEMNGQKIEK
MNDVAPFIQKAGKTGESLDLLIKRDKQKIKTKLIPEKDEGEGKYRIGLYIRDSAAGIGTMTFYEPKTKKYGALGHVISDM
DTKKPIVVENGEIVKSTVTSIEKGTGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLHQPIQNNISDQALPVAFSTEVK
KGPAEILTVIDDDKVEKFDIEIVSTTPQKFPATKGMVLKITDPRLLKETGGIVQGMSGSPIIQNGKVIGAVTHVFVNDPT
SGYGVHIEWMLSEAGIDIYGKEKAS

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SP4B_BACSU (P17896)

Other databases:

- EMBL:   M30297
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   C35128
- RefSeq:   NP_390303.2
- ProteinModelPortal:   P17896
- SMR:   P17896
- MEROPS:   S55.001
- EnsemblBacteria:   EBBACT00000003786
- GeneID:   938654
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24230
- NMPDR:   fig|224308.1.peg.2427
- GenoList:   BSU24230
- GeneTree:   EBGT00050000001568
- HOGENOM:   HBG398945
- ProtClustDB:   CLSK873421
- BioCyc:   BSUB:BSU24230-MONOMER
- BRENDA:   3.4.21.116
- PMAP-CutDB:   P17896
- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763
- SMART:   SM00228
- TIGRFAMs:   TIGR02860

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55; SSF50156 PDZ

EC number: =3.4.21.116

Molecular weight: Translated: 46076; Mature: 45945

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50106 PDZ; PS51494 SPOIVB

Important sites: ACT_SITE 236-236 ACT_SITE 363-363 ACT_SITE 378-378

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNIRKAVGLILLVSLLSVGLCKPLKEYLLIPTQMRVFETQTQAIETSLSVNAQTSESS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCC
EAFTVKKDPHEIKVTGKKSGESELVYDLAGFPIKKTKVHVLPDLKVIPGGQSIGVKLHSV
CEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEECCCCEEECCCCEEEEEEEEE
GVLVVGFHQINTSEGKKSPGETAGIEAGDIIIEMNGQKIEKMNDVAPFIQKAGKTGESLD
EEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCH
LLIKRDKQKIKTKLIPEKDEGEGKYRIGLYIRDSAAGIGTMTFYEPKTKKYGALGHVISD
HEEHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHC
MDTKKPIVVENGEIVKSTVTSIEKGTGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLH
CCCCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEHHCC
QPIQNNISDQALPVAFSTEVKKGPAEILTVIDDDKVEKFDIEIVSTTPQKFPATKGMVLK
HHHHCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEE
ITDPRLLKETGGIVQGMSGSPIIQNGKVIGAVTHVFVNDPTSGYGVHIEWMLSEAGIDIY
ECCCHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEECCCEEEE
GKEKAS
CCCCCC
>Mature Secondary Structure 
PDNIRKAVGLILLVSLLSVGLCKPLKEYLLIPTQMRVFETQTQAIETSLSVNAQTSESS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCC
EAFTVKKDPHEIKVTGKKSGESELVYDLAGFPIKKTKVHVLPDLKVIPGGQSIGVKLHSV
CEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEECCCCEEECCCCEEEEEEEEE
GVLVVGFHQINTSEGKKSPGETAGIEAGDIIIEMNGQKIEKMNDVAPFIQKAGKTGESLD
EEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCH
LLIKRDKQKIKTKLIPEKDEGEGKYRIGLYIRDSAAGIGTMTFYEPKTKKYGALGHVISD
HEEHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHC
MDTKKPIVVENGEIVKSTVTSIEKGTGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLH
CCCCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEHHCC
QPIQNNISDQALPVAFSTEVKKGPAEILTVIDDDKVEKFDIEIVSTTPQKFPATKGMVLK
HHHHCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEE
ITDPRLLKETGGIVQGMSGSPIIQNGKVIGAVTHVFVNDPTSGYGVHIEWMLSEAGIDIY
ECCCHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEECCCEEEE
GKEKAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860