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The map label for this gene is spo0A

Identifier: 16079478

GI number: 16079478

Start: 2518023

End: 2518826

Strand: Reverse

Name: spo0A

Synonym: BSU24220

Alternate gene names: 16079478

Gene position: 2518826-2518023 (Counterclockwise)

Preceding gene: 255767535

Following gene: 255767534

Centisome position: 59.75

GC content: 45.02

Gene sequence:

>804_bases
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTGGTAAGCCTGTTAAGTGAATATATAGAAGGACAGGA
AGACATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGCCTGTCGCTGTTTAAAGAAAAAGATCCCGATGTGCTCG
TATTAGATATTATTATGCCGCATCTAGACGGACTTGCGGTTTTAGAGAGGCTGAGGGAATCAGATCTGAAAAAACAGCCG
AATGTCATTATGCTGACAGCCTTTGGGCAGGAAGATGTCACGAAAAAGGCCGTCGATTTAGGCGCGTCCTACTTTATTCT
CAAACCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTCAGCGGAAATGCCAGCAGTGTGACGCATCGTGCGC
CATCATCGCAAAGCAGTATTATACGCAGCAGCCAGCCTGAACCAAAGAAGAAAAATCTCGACGCGAGCATCACAAGCATT
ATCCATGAAATCGGCGTCCCAGCCCATATTAAAGGCTATCTCTATCTGCGCGAAGCAATCTCAATGGTATACAATGACAT
CGAATTGCTCGGCAGCATTACAAAAGTCCTCTATCCGGACATCGCCAAAAAATTTAACACAACCGCAAGCCGTGTAGAAA
GAGCGATCCGCCATGCAATTGAAGTGGCATGGAGCAGAGGAAACATTGATTCCATTTCCTCGTTGTTTGGTTATACTGTC
AGCATGACAAAAGCTAAACCTACCAACAGTGAATTCATTGCAATGGTTGCGGATAAGCTGAGGTTAGAGCATAAGGCTTC
TTAA

Upstream 100 bases:

>100_bases
TTGGTGATTATGATTTTTTTAGAGGGTATATAGCGGTTTTGTCGAATGTAAACATGTAGCAAGGGTGAATCCTGTTAACT
ACATTTGGGGAGGAAGAAAC

Downstream 100 bases:

>100_bases
ACATGAGCTTATTAAGTGGTCATTAAATCAAACGTCTTTTATTTATTAGTTTGCGCTGATAAATAGGAGGCGTTTTGTTT
TGGGGACATTTGTAGTATGG

Product: response regulator

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: Stage 0 sporulation protein C; Stage 0 sporulation protein G

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MEKIKVCVADDNRELVSLLSEYIEGQEDMEVIGVAYNGQECLSLFKEKDPDVLVLDIIMPHLDGLAVLERLRESDLKKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNASSVTHRAPSSQSSIIRSSQPEPKKKNLDASITSI
IHEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
SMTKAKPTNSEFIAMVADKLRLEHKAS

Sequences:

>Translated_267_residues
MEKIKVCVADDNRELVSLLSEYIEGQEDMEVIGVAYNGQECLSLFKEKDPDVLVLDIIMPHLDGLAVLERLRESDLKKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNASSVTHRAPSSQSSIIRSSQPEPKKKNLDASITSI
IHEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
SMTKAKPTNSEFIAMVADKLRLEHKAS
>Mature_267_residues
MEKIKVCVADDNRELVSLLSEYIEGQEDMEVIGVAYNGQECLSLFKEKDPDVLVLDIIMPHLDGLAVLERLRESDLKKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNASSVTHRAPSSQSSIIRSSQPEPKKKNLDASITSI
IHEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
SMTKAKPTNSEFIAMVADKLRLEHKAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=111, Percent_Identity=39.6396396396396, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789809, Length=140, Percent_Identity=33.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=30.9734513274336, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SP0A_BACSU (P06534)

Other databases:

- EMBL:   M10082
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   U68235
- EMBL:   M23656
- PIR:   A94036
- RefSeq:   NP_390302.1
- PDB:   1LQ1
- PDBsum:   1LQ1
- ProteinModelPortal:   P06534
- EnsemblBacteria:   EBBACT00000000284
- GeneID:   938655
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24220
- NMPDR:   fig|224308.1.peg.2426
- GenoList:   BSU24220
- GeneTree:   EBGT00070000031787
- HOGENOM:   HBG304664
- OMA:   AHIKGYL
- ProtClustDB:   CLSK873420
- BioCyc:   BSUB:BSU24220-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF002937
- ProDom:   PD010908
- SMART:   SM00448
- TIGRFAMs:   TIGR02875

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29692; Mature: 29692

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVCVADDNRELVSLLSEYIEGQEDMEVIGVAYNGQECLSLFKEKDPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCCEEEHHHHHH
HLDGLAVLERLRESDLKKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGNASSVTHRAPSSQSSIIRSSQPEPKKKNLDASITSIIHEIGVPAHIKGYLYLREAI
HHCCCCCHHCCCCCCCHHHHHHCCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
SMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHE
SMTKAKPTNSEFIAMVADKLRLEHKAS
EEECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIKVCVADDNRELVSLLSEYIEGQEDMEVIGVAYNGQECLSLFKEKDPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCCEEEHHHHHH
HLDGLAVLERLRESDLKKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGNASSVTHRAPSSQSSIIRSSQPEPKKKNLDASITSIIHEIGVPAHIKGYLYLREAI
HHCCCCCHHCCCCCCCHHHHHHCCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
SMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTV
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHE
SMTKAKPTNSEFIAMVADKLRLEHKAS
EEECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3921963; 3018427; 3157992; 8969508; 9384377; 9044256; 3151993; 2121995; 12176382