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The map label for this gene is ptb

Identifier: 255767528

GI number: 255767528

Start: 2503765

End: 2504664

Strand: Reverse

Name: ptb

Synonym: BSU24090

Alternate gene names: 255767528

Gene position: 2504664-2503765 (Counterclockwise)

Preceding gene: 16079466

Following gene: 16079464

Centisome position: 59.41

GC content: 48.11

Gene sequence:

>900_bases
ATGAAGCTGAAAGATTTAATCGGCAAAGCGTCGATACACAAAAATAAGACCATTGCGGTTGCTCATGCTGAGGATGAGGA
AGTGATCCGGGCTGTAAAGCTGGCAGCCGAGCACCTATCCGCCCGTTTCTTGTTAACGGGCGACAGCAAAAAACTGAACG
AGCTGACATCATCCATGCAAGGGCATCAAGTGGAAATCGTCCATGCCAATACTCCTGAGGAATCGGCGAAATTAGCGGTT
CGTGCTGTGCATCATAAAACAGCGGACGTTTTAATGAAAGGCAATGTGCCGACTTCTGTTTTGCTTAAAGCGGTATTGAA
CAGACAGGAGGGGCTTCGGTCAGCAAGTGTCCTTTCTCACGTGGCTGTCTTCGATATACCGGATTTTGACCGGCTGATGT
TTGTGACAGATTCGGCAATGAATATTGCTCCTTCGCTGGAAGAGCTCCGGCAGATTTTGCAAAATGCCGTTCATGTGGCC
CATGCAGTGGGGAACAATATGCCAAAAGCAGCTGCCCTTGCAGCGGTCGAAACGGTGAACCCTAAAATGGAAGCGACCGT
GAATGCAGCTGCTCTAGCTCAAATGTACAAAAGGGGCCAGATCAAAGGCTGCATTGTTGATGGACCGCTTGCTTTGGATA
ACGCAGTTTCACAAATTGCCGCGGCCCAGAAAAAAATTTCGGGAGACGTAGCGGGCAACGCAGATATCCTTCTCGTCCCA
ACAATTGAGGCCGGCAATATTTTATATAAATCACTGATTTATTTTGCCAAGGCCAGCGTGGCGGCTGTCATTACAGGAGC
AAAAGCACCGATTGCTTTAACAAGCAGAGCGGACTCTGCTGAAAATAAACTGTATTCCATTGCGCTGGCAATATGCGCAT
CTGAAGAATACACACATTAG

Upstream 100 bases:

>100_bases
GCACGAATGTTTCGGTATACCTGGTATGACAGCACCCTTAAGAGCTGGCATGGAACTTGCATAATAAAAGGCGGAGTCGA
AACAAGAAAGGTGGTAACAG

Downstream 100 bases:

>100_bases
GAGGAACGAACATGGAACTTTTTAAATATATGGAGAAATACGATTACGAACAATTGGTATTCTGCCAGGATGAACAATCT
GGATTAAAAGCGATTATCGC

Product: phosphate butyryltransferase

Products: NA

Alternate protein names: Phosphotransbutyrylase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKLKDLIGKASIHKNKTIAVAHAEDEEVIRAVKLAAEHLSARFLLTGDSKKLNELTSSMQGHQVEIVHANTPEESAKLAV
RAVHHKTADVLMKGNVPTSVLLKAVLNRQEGLRSASVLSHVAVFDIPDFDRLMFVTDSAMNIAPSLEELRQILQNAVHVA
HAVGNNMPKAAALAAVETVNPKMEATVNAAALAQMYKRGQIKGCIVDGPLALDNAVSQIAAAQKKISGDVAGNADILLVP
TIEAGNILYKSLIYFAKASVAAVITGAKAPIALTSRADSAENKLYSIALAICASEEYTH

Sequences:

>Translated_299_residues
MKLKDLIGKASIHKNKTIAVAHAEDEEVIRAVKLAAEHLSARFLLTGDSKKLNELTSSMQGHQVEIVHANTPEESAKLAV
RAVHHKTADVLMKGNVPTSVLLKAVLNRQEGLRSASVLSHVAVFDIPDFDRLMFVTDSAMNIAPSLEELRQILQNAVHVA
HAVGNNMPKAAALAAVETVNPKMEATVNAAALAQMYKRGQIKGCIVDGPLALDNAVSQIAAAQKKISGDVAGNADILLVP
TIEAGNILYKSLIYFAKASVAAVITGAKAPIALTSRADSAENKLYSIALAICASEEYTH
>Mature_299_residues
MKLKDLIGKASIHKNKTIAVAHAEDEEVIRAVKLAAEHLSARFLLTGDSKKLNELTSSMQGHQVEIVHANTPEESAKLAV
RAVHHKTADVLMKGNVPTSVLLKAVLNRQEGLRSASVLSHVAVFDIPDFDRLMFVTDSAMNIAPSLEELRQILQNAVHVA
HAVGNNMPKAAALAAVETVNPKMEATVNAAALAQMYKRGQIKGCIVDGPLALDNAVSQIAAAQKKISGDVAGNADILLVP
TIEAGNILYKSLIYFAKASVAAVITGAKAPIALTSRADSAENKLYSIALAICASEEYTH

Specific function: Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family

Homologues:

None

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PTB_BACSU (P54530)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A69962
- RefSeq:   NP_390289.2
- ProteinModelPortal:   P54530
- SMR:   P54530
- EnsemblBacteria:   EBBACT00000000894
- GeneID:   938671
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24090
- NMPDR:   fig|224308.1.peg.2413
- GenoList:   BSU24090
- GeneTree:   EBGT00070000032020
- HOGENOM:   HBG294533
- ProtClustDB:   PRK07742
- BioCyc:   BSUB:BSU24090-MONOMER
- BRENDA:   2.3.1.19
- InterPro:   IPR012147
- InterPro:   IPR014079
- InterPro:   IPR002505
- PIRSF:   PIRSF000428
- TIGRFAMs:   TIGR02706

Pfam domain/function: PF01515 PTA_PTB

EC number: =2.3.1.19

Molecular weight: Translated: 31747; Mature: 31747

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKDLIGKASIHKNKTIAVAHAEDEEVIRAVKLAAEHLSARFLLTGDSKKLNELTSSMQ
CCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCC
GHQVEIVHANTPEESAKLAVRAVHHKTADVLMKGNVPTSVLLKAVLNRQEGLRSASVLSH
CCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHH
VAVFDIPDFDRLMFVTDSAMNIAPSLEELRQILQNAVHVAHAVGNNMPKAAALAAVETVN
HHEECCCCCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PKMEATVNAAALAQMYKRGQIKGCIVDGPLALDNAVSQIAAAQKKISGDVAGNADILLVP
CCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TIEAGNILYKSLIYFAKASVAAVITGAKAPIALTSRADSAENKLYSIALAICASEEYTH
CCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MKLKDLIGKASIHKNKTIAVAHAEDEEVIRAVKLAAEHLSARFLLTGDSKKLNELTSSMQ
CCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCC
GHQVEIVHANTPEESAKLAVRAVHHKTADVLMKGNVPTSVLLKAVLNRQEGLRSASVLSH
CCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHHH
VAVFDIPDFDRLMFVTDSAMNIAPSLEELRQILQNAVHVAHAVGNNMPKAAALAAVETVN
HHEECCCCCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PKMEATVNAAALAQMYKRGQIKGCIVDGPLALDNAVSQIAAAQKKISGDVAGNADILLVP
CCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TIEAGNILYKSLIYFAKASVAAVITGAKAPIALTSRADSAENKLYSIALAICASEEYTH
CCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377