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The map label for this gene is bcd

Identifier: 16079464

GI number: 16079464

Start: 2502659

End: 2503753

Strand: Reverse

Name: bcd

Synonym: BSU24080

Alternate gene names: 16079464

Gene position: 2503753-2502659 (Counterclockwise)

Preceding gene: 255767528

Following gene: 255767527

Centisome position: 59.39

GC content: 47.31

Gene sequence:

>1095_bases
ATGGAACTTTTTAAATATATGGAGAAATACGATTACGAACAATTGGTATTCTGCCAGGATGAACAATCTGGATTAAAAGC
GATTATCGCCATTCATGATACAACGCTTGGTCCGGCGCTTGGCGGAACGAGAATGTGGACATATGAAAATGAAGAAGCGG
CAATTGAAGATGCGCTCAGATTGGCAAGAGGCATGACCTATAAGAACGCGGCGGCAGGCTTAAACCTTGGCGGCGGAAAA
ACAGTCATTATCGGCGATCCGCGCAAAGACAAAAATGAGGAAATGTTCCGCGCGTTTGGCCGCTATATTCAAGGACTGAA
TGGCAGATACATCACGGCTGAAGATGTGGGCACAACGGTCGAGGATATGGATATCATTCATGATGAGACAGACTATGTCA
CAGGGATTTCTCCTGCTTTCGGCTCTTCTGGAAATCCGTCCCCAGTCACAGCGTACGGGGTGTACAGAGGAATGAAGGCA
GCAGCTAAAGCTGCTTTCGGAACCGACTCTCTTGAAGGAAAAACCATTGCTGTACAGGGTGTTGGGAACGTAGCCTATAA
CCTTTGCCGCCACCTGCATGAAGAAGGAGCAAACTTAATCGTTACGGATATCAACAAACAATCTGTACAGCGTGCAGTTG
AAGATTTTGGCGCCCGTGCGGTAGATCCTGATGACATTTATTCACAAGACTGCGATATTTATGCGCCGTGTGCCCTTGGT
GCGACTATTAACGACGACACCATTAAACAGCTGAAGGCGAAAGTGATCGCAGGTGCGGCTAACAACCAATTAAAAGAGAC
ACGCCATGGTGATCAAATTCACGAAATGGGCATCGTTTATGCACCGGATTACGTGATTAACGCGGGCGGTGTCATCAACG
TGGCAGATGAGCTTTACGGCTATAATGCAGAACGTGCATTGAAAAAAGTTGAAGGCATTTACGGCAATATCGAGCGTGTA
CTTGAGATTTCTCAGCGTGACGGCATTCCTGCATATTTAGCGGCTGACCGCTTAGCAGAGGAACGGATTGAACGCATGCG
CCGCTCAAGAAGCCAGTTTTTGCAAAACGGCCACAGTGTATTAAGCAGACGTTAA

Upstream 100 bases:

>100_bases
TTGCTTTAACAAGCAGAGCGGACTCTGCTGAAAATAAACTGTATTCCATTGCGCTGGCAATATGCGCATCTGAAGAATAC
ACACATTAGGAGGAACGAAC

Downstream 100 bases:

>100_bases
GAAATTGATCTGGAGGTTATGAAAGTGCTACATGACGAAAAACGCATTCTCACAATTAATCCAGGCTCAACTTCAACGAA
AATCGGTGTTTTTCATAACG

Product: branched-chain amino acid dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MELFKYMEKYDYEQLVFCQDEQSGLKAIIAIHDTTLGPALGGTRMWTYENEEAAIEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTVEDMDIIHDETDYVTGISPAFGSSGNPSPVTAYGVYRGMKA
AAKAAFGTDSLEGKTIAVQGVGNVAYNLCRHLHEEGANLIVTDINKQSVQRAVEDFGARAVDPDDIYSQDCDIYAPCALG
ATINDDTIKQLKAKVIAGAANNQLKETRHGDQIHEMGIVYAPDYVINAGGVINVADELYGYNAERALKKVEGIYGNIERV
LEISQRDGIPAYLAADRLAEERIERMRRSRSQFLQNGHSVLSRR

Sequences:

>Translated_364_residues
MELFKYMEKYDYEQLVFCQDEQSGLKAIIAIHDTTLGPALGGTRMWTYENEEAAIEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTVEDMDIIHDETDYVTGISPAFGSSGNPSPVTAYGVYRGMKA
AAKAAFGTDSLEGKTIAVQGVGNVAYNLCRHLHEEGANLIVTDINKQSVQRAVEDFGARAVDPDDIYSQDCDIYAPCALG
ATINDDTIKQLKAKVIAGAANNQLKETRHGDQIHEMGIVYAPDYVINAGGVINVADELYGYNAERALKKVEGIYGNIERV
LEISQRDGIPAYLAADRLAEERIERMRRSRSQFLQNGHSVLSRR
>Mature_364_residues
MELFKYMEKYDYEQLVFCQDEQSGLKAIIAIHDTTLGPALGGTRMWTYENEEAAIEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTVEDMDIIHDETDYVTGISPAFGSSGNPSPVTAYGVYRGMKA
AAKAAFGTDSLEGKTIAVQGVGNVAYNLCRHLHEEGANLIVTDINKQSVQRAVEDFGARAVDPDDIYSQDCDIYAPCALG
ATINDDTIKQLKAKVIAGAANNQLKETRHGDQIHEMGIVYAPDYVINAGGVINVADELYGYNAERALKKVEGIYGNIERV
LEISQRDGIPAYLAADRLAEERIERMRRSRSQFLQNGHSVLSRR

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=305, Percent_Identity=27.8688524590164, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4885281, Length=305, Percent_Identity=27.8688524590164, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17544676, Length=312, Percent_Identity=25.9615384615385, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24649283, Length=297, Percent_Identity=27.9461279461279, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 39992; Mature: 39992

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFKYMEKYDYEQLVFCQDEQSGLKAIIAIHDTTLGPALGGTRMWTYENEEAAIEDALR
CHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTV
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCH
EDMDIIHDETDYVTGISPAFGSSGNPSPVTAYGVYRGMKAAAKAAFGTDSLEGKTIAVQG
HHHHHHCCCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
VGNVAYNLCRHLHEEGANLIVTDINKQSVQRAVEDFGARAVDPDDIYSQDCDIYAPCALG
CHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECEECC
ATINDDTIKQLKAKVIAGAANNQLKETRHGDQIHEMGIVYAPDYVINAGGVINVADELYG
CCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCEECCCEEEECCCEEEEHHHHHC
YNAERALKKVEGIYGNIERVLEISQRDGIPAYLAADRLAEERIERMRRSRSQFLQNGHSV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRR
HCCC
>Mature Secondary Structure
MELFKYMEKYDYEQLVFCQDEQSGLKAIIAIHDTTLGPALGGTRMWTYENEEAAIEDALR
CHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTV
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCH
EDMDIIHDETDYVTGISPAFGSSGNPSPVTAYGVYRGMKAAAKAAFGTDSLEGKTIAVQG
HHHHHHCCCCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
VGNVAYNLCRHLHEEGANLIVTDINKQSVQRAVEDFGARAVDPDDIYSQDCDIYAPCALG
CHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECEECC
ATINDDTIKQLKAKVIAGAANNQLKETRHGDQIHEMGIVYAPDYVINAGGVINVADELYG
CCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCEECCCEEEECCCEEEEHHHHHC
YNAERALKKVEGIYGNIERVLEISQRDGIPAYLAADRLAEERIERMRRSRSQFLQNGHSV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8597545 [H]