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The map label for this gene is buk

Identifier: 255767527

GI number: 255767527

Start: 2501549

End: 2502640

Strand: Reverse

Name: buk

Synonym: BSU24070

Alternate gene names: 255767527

Gene position: 2502640-2501549 (Counterclockwise)

Preceding gene: 16079464

Following gene: 255767526

Centisome position: 59.37

GC content: 45.6

Gene sequence:

>1092_bases
ATGAAAGTGCTACATGACGAAAAACGCATTCTCACAATTAATCCAGGCTCAACTTCAACGAAAATCGGTGTTTTTCATAA
CGAAAGAAGCATTTTTGAAAAAACATTGAGACACAACATAGAAGAGCTGCAGCGATTTGACCGAATTATTGATCAGTATG
AATTTCGTAAAAACCATATTCTCGAAACGCTTCATGAACAGGGAATCAATATTTCAAAGTTTGATGCAGTCTGCGCAAGA
GGCGGCCTTCTCCGGCCAATTGAAGGCGGGACCTACGTAGTGAATGATGAGATGATTGAGGATTTGAAAAGCGGCTACGC
CGGTCAGCATGCTTCTAATCTCGGCGGCATTATCGCGCGGGAAATCGCTGACGGCCTGAATATCCCTTCGTATATCGTCG
ACCCTGTTGTCGTAGACGAAATGTCAGTGCTTGCGAAAGTTTCAGGCATGCCTGAAATTGAAAGGAAAAGCATTTTCCAT
GCGTTAAACCAAAAAGCTGTCGCTCGTAAAGCGGCAGCCTCCCTTGGCAAACGATATGAGAACATGAAAATGATTATTAC
CCATATGGGCGGAGGTATTACAATTGGCGTCCATGACCGCGGGCGAGTGGTGGATGTCAATAACGGCCTGCACGGCGAGG
GACCATTCAGTCCTGAACGAGCAGGAACAGTGCCTGCGGGAGATCTTGTTGATCTATGTTTTTCAGGGGAATATACAAAA
GAAGAAATCATGAAAAAGCTTGTTGGCACCGGCGGACTTTTAGGCTATCTCGGTACAAATGATGCAGTAAAAGTGGAACA
GATGATTCAAGGGGGCGATGAAAAAGCCCGCTTCATTTATGATGCCATGGCATATCAGGTAGCAAAGGAAATCGGGGCAG
CCAGCGCGGCGTTAAAAGGCGAAGTTGAAGCGATTGTGCTGACGGGAGGCCTTGCGTACGGAAAAAGCTTTGTTTCTGCT
ATCAGATCATACATAGATTGGATTTCAGACGTTTTAGTTTATCCGGGTGAAAATGAACTGCAGTCTCTTGCCCAAGGGGC
GCTAAGAGTATTGCAGGGCGAAGAGCAATCCAAACAATATCGTATTGAATAG

Upstream 100 bases:

>100_bases
AGCAGAGGAACGGATTGAACGCATGCGCCGCTCAAGAAGCCAGTTTTTGCAAAACGGCCACAGTGTATTAAGCAGACGTT
AAGAAATTGATCTGGAGGTT

Downstream 100 bases:

>100_bases
ACAGACAGGAGTGAGTCACCATGGCAACTGAGTATGACGTAGTCATTCTGGGCGGCGGTACCGGCGGTTATGTTGCGGCC
ATCAGAGCCGCTCAGCTCGG

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKVLHDEKRILTINPGSTSTKIGVFHNERSIFEKTLRHNIEELQRFDRIIDQYEFRKNHILETLHEQGINISKFDAVCAR
GGLLRPIEGGTYVVNDEMIEDLKSGYAGQHASNLGGIIAREIADGLNIPSYIVDPVVVDEMSVLAKVSGMPEIERKSIFH
ALNQKAVARKAAASLGKRYENMKMIITHMGGGITIGVHDRGRVVDVNNGLHGEGPFSPERAGTVPAGDLVDLCFSGEYTK
EEIMKKLVGTGGLLGYLGTNDAVKVEQMIQGGDEKARFIYDAMAYQVAKEIGAASAALKGEVEAIVLTGGLAYGKSFVSA
IRSYIDWISDVLVYPGENELQSLAQGALRVLQGEEQSKQYRIE

Sequences:

>Translated_363_residues
MKVLHDEKRILTINPGSTSTKIGVFHNERSIFEKTLRHNIEELQRFDRIIDQYEFRKNHILETLHEQGINISKFDAVCAR
GGLLRPIEGGTYVVNDEMIEDLKSGYAGQHASNLGGIIAREIADGLNIPSYIVDPVVVDEMSVLAKVSGMPEIERKSIFH
ALNQKAVARKAAASLGKRYENMKMIITHMGGGITIGVHDRGRVVDVNNGLHGEGPFSPERAGTVPAGDLVDLCFSGEYTK
EEIMKKLVGTGGLLGYLGTNDAVKVEQMIQGGDEKARFIYDAMAYQVAKEIGAASAALKGEVEAIVLTGGLAYGKSFVSA
IRSYIDWISDVLVYPGENELQSLAQGALRVLQGEEQSKQYRIE
>Mature_363_residues
MKVLHDEKRILTINPGSTSTKIGVFHNERSIFEKTLRHNIEELQRFDRIIDQYEFRKNHILETLHEQGINISKFDAVCAR
GGLLRPIEGGTYVVNDEMIEDLKSGYAGQHASNLGGIIAREIADGLNIPSYIVDPVVVDEMSVLAKVSGMPEIERKSIFH
ALNQKAVARKAAASLGKRYENMKMIITHMGGGITIGVHDRGRVVDVNNGLHGEGPFSPERAGTVPAGDLVDLCFSGEYTK
EEIMKKLVGTGGLLGYLGTNDAVKVEQMIQGGDEKARFIYDAMAYQVAKEIGAASAALKGEVEAIVLTGGLAYGKSFVSA
IRSYIDWISDVLVYPGENELQSLAQGALRVLQGEEQSKQYRIE

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

None

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): BUK_BACSU (P54532)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   C69962
- RefSeq:   NP_390287.2
- ProteinModelPortal:   P54532
- SMR:   P54532
- EnsemblBacteria:   EBBACT00000003746
- GeneID:   938675
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24070
- NMPDR:   fig|224308.1.peg.2411
- GenoList:   BSU24070
- GeneTree:   EBGT00050000000769
- HOGENOM:   HBG449785
- ProtClustDB:   PRK03011
- BioCyc:   BSUB:BSU24070-MONOMER
- BRENDA:   2.7.2.7
- GO:   GO:0005737
- HAMAP:   MF_00542
- InterPro:   IPR000890
- InterPro:   IPR011245
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF3
- PIRSF:   PIRSF036458
- PRINTS:   PR00471
- TIGRFAMs:   TIGR02707

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.7

Molecular weight: Translated: 39764; Mature: 39764

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLHDEKRILTINPGSTSTKIGVFHNERSIFEKTLRHNIEELQRFDRIIDQYEFRKNHI
CCCCCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETLHEQGINISKFDAVCARGGLLRPIEGGTYVVNDEMIEDLKSGYAGQHASNLGGIIAR
HHHHHHCCCCHHHHHHHHHCCCCEEECCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHHH
EIADGLNIPSYIVDPVVVDEMSVLAKVSGMPEIERKSIFHALNQKAVARKAAASLGKRYE
HHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NMKMIITHMGGGITIGVHDRGRVVDVNNGLHGEGPFSPERAGTVPAGDLVDLCFSGEYTK
HHHEEEEECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCH
EEIMKKLVGTGGLLGYLGTNDAVKVEQMIQGGDEKARFIYDAMAYQVAKEIGAASAALKG
HHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVEAIVLTGGLAYGKSFVSAIRSYIDWISDVLVYPGENELQSLAQGALRVLQGEEQSKQY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCC
RIE
CCC
>Mature Secondary Structure
MKVLHDEKRILTINPGSTSTKIGVFHNERSIFEKTLRHNIEELQRFDRIIDQYEFRKNHI
CCCCCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETLHEQGINISKFDAVCARGGLLRPIEGGTYVVNDEMIEDLKSGYAGQHASNLGGIIAR
HHHHHHCCCCHHHHHHHHHCCCCEEECCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHHH
EIADGLNIPSYIVDPVVVDEMSVLAKVSGMPEIERKSIFHALNQKAVARKAAASLGKRYE
HHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NMKMIITHMGGGITIGVHDRGRVVDVNNGLHGEGPFSPERAGTVPAGDLVDLCFSGEYTK
HHHEEEEECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCH
EEIMKKLVGTGGLLGYLGTNDAVKVEQMIQGGDEKARFIYDAMAYQVAKEIGAASAALKG
HHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVEAIVLTGGLAYGKSFVSAIRSYIDWISDVLVYPGENELQSLAQGALRVLQGEEQSKQY
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCC
RIE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377