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The map label for this gene is lpdV

Identifier: 255767526

GI number: 255767526

Start: 2500104

End: 2501528

Strand: Reverse

Name: lpdV

Synonym: BSU24060

Alternate gene names: 255767526

Gene position: 2501528-2500104 (Counterclockwise)

Preceding gene: 255767527

Following gene: 16079461

Centisome position: 59.34

GC content: 48.07

Gene sequence:

>1425_bases
ATGGCAACTGAGTATGACGTAGTCATTCTGGGCGGCGGTACCGGCGGTTATGTTGCGGCCATCAGAGCCGCTCAGCTCGG
CTTAAAAACAGCCGTTGTGGAAAAGGAAAAACTCGGGGGAACATGTCTGCATAAAGGCTGTATCCCGAGTAAAGCGCTGC
TTAGAAGCGCAGAGGTATACCGGACAGCTCGTGAAGCCGATCAATTCGGAGTGGAAACGGCTGGCGTGTCCCTCAACTTT
GAAAAAGTGCAGCAGCGTAAGCAAGCCGTTGTTGATAAGCTTGCAGCGGGTGTAAATCATTTAATGAAAAAAGGAAAAAT
TGACGTGTACACCGGATATGGACGTATCCTTGGACCGTCAATCTTCTCTCCGCTGCCGGGAACAATTTCTGTTGAGCGGG
GAAATGGCGAAGAAAATGACATGCTGATCCCGAAACAAGTGATCATTGCAACAGGATCAAGACCGAGAATGCTTCCGGGT
CTTGAAGTGGACGGTAAGTCTGTACTGACTTCAGATGAGGCGCTCCAAATGGAGGAGCTGCCACAGTCAATCATCATTGT
CGGCGGAGGGGTTATCGGTATCGAATGGGCGTCTATGCTTCATGATTTTGGCGTTAAGGTAACGGTTATTGAATACGCGG
ATCGCATATTGCCGACTGAAGATCTAGAGATTTCAAAAGAAATGGAAAGTCTTCTTAAGAAAAAAGGCATCCAGTTCATA
ACAGGGGCAAAAGTGCTGCCTGACACAATGACAAAAACATCAGACGATATCAGCATACAAGCGGAAAAAGACGGAGAAAC
CGTTACCTATTCTGCTGAGAAAATGCTTGTTTCCATCGGCAGACAGGCAAATATCGAAGGCATCGGCCTAGAGAACACCG
ATATTGTTACTGAAAATGGCATGATTTCAGTCAATGAAAGCTGCCAAACGAAGGAATCTCATATTTATGCAATCGGAGAC
GTAATCGGTGGCCTGCAGTTAGCTCACGTTGCTTCACATGAGGGAATTATTGCTGTTGAGCATTTTGCAGGTCTCAATCC
GCATCCGCTTGATCCGACGCTTGTGCCGAAGTGCATTTACTCAAGCCCTGAAGCTGCCAGTGTCGGCTTAACCGAAGACG
AAGCAAAGGCGAACGGGCATAATGTCAAAATCGGCAAGTTCCCATTTATGGCGATTGGAAAAGCGCTTGTATACGGTGAA
AGCGACGGTTTTGTCAAAATCGTGGCTGACCGAGATACAGATGATATTCTCGGCGTTCATATGATTGGCCCGCATGTCAC
CGACATGATTTCTGAAGCGGGTCTTGCCAAAGTGCTGGACGCAACACCGTGGGAGGTCGGGCAAACGATTCACCCGCATC
CAACGCTTTCTGAAGCAATTGGAGAAGCTGCGCTTGCCGCAGATGGCAAAGCCATTCATTTTTAA

Upstream 100 bases:

>100_bases
ATGAACTGCAGTCTCTTGCCCAAGGGGCGCTAAGAGTATTGCAGGGCGAAGAGCAATCCAAACAATATCGTATTGAATAG
ACAGACAGGAGTGAGTCACC

Downstream 100 bases:

>100_bases
AAGCATAAAGGAGGGGCTTGAATGAGTACAAACCGACATCAAGCACTAGGGCTGACTGATCAGGAAGCCGTTGATATGTA
TAGAACCATGCTGTTAGCAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MATEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKEKLGGTCLHKGCIPSKALLRSAEVYRTAREADQFGVETAGVSLNF
EKVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPSIFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRMLPG
LEVDGKSVLTSDEALQMEELPQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILPTEDLEISKEMESLLKKKGIQFI
TGAKVLPDTMTKTSDDISIQAEKDGETVTYSAEKMLVSIGRQANIEGIGLENTDIVTENGMISVNESCQTKESHIYAIGD
VIGGLQLAHVASHEGIIAVEHFAGLNPHPLDPTLVPKCIYSSPEAASVGLTEDEAKANGHNVKIGKFPFMAIGKALVYGE
SDGFVKIVADRDTDDILGVHMIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAADGKAIHF

Sequences:

>Translated_474_residues
MATEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKEKLGGTCLHKGCIPSKALLRSAEVYRTAREADQFGVETAGVSLNF
EKVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPSIFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRMLPG
LEVDGKSVLTSDEALQMEELPQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILPTEDLEISKEMESLLKKKGIQFI
TGAKVLPDTMTKTSDDISIQAEKDGETVTYSAEKMLVSIGRQANIEGIGLENTDIVTENGMISVNESCQTKESHIYAIGD
VIGGLQLAHVASHEGIIAVEHFAGLNPHPLDPTLVPKCIYSSPEAASVGLTEDEAKANGHNVKIGKFPFMAIGKALVYGE
SDGFVKIVADRDTDDILGVHMIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAADGKAIHF
>Mature_473_residues
ATEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKEKLGGTCLHKGCIPSKALLRSAEVYRTAREADQFGVETAGVSLNFE
KVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPSIFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRMLPGL
EVDGKSVLTSDEALQMEELPQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILPTEDLEISKEMESLLKKKGIQFIT
GAKVLPDTMTKTSDDISIQAEKDGETVTYSAEKMLVSIGRQANIEGIGLENTDIVTENGMISVNESCQTKESHIYAIGDV
IGGLQLAHVASHEGIIAVEHFAGLNPHPLDPTLVPKCIYSSPEAASVGLTEDEAKANGHNVKIGKFPFMAIGKALVYGES
DGFVKIVADRDTDDILGVHMIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAADGKAIHF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=41.0041841004184, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=30.3225806451613, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI291045266, Length=483, Percent_Identity=26.5010351966874, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI22035672, Length=485, Percent_Identity=27.4226804123711, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI33519430, Length=482, Percent_Identity=25.103734439834, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI33519428, Length=482, Percent_Identity=25.103734439834, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI33519426, Length=482, Percent_Identity=25.103734439834, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI148277071, Length=480, Percent_Identity=25.2083333333333, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI148277065, Length=482, Percent_Identity=25.103734439834, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI291045268, Length=480, Percent_Identity=25.2083333333333, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786307, Length=483, Percent_Identity=34.1614906832298, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=27.3684210526316, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=28.3582089552239, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789915, Length=448, Percent_Identity=28.5714285714286, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=41.4376321353066, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17557007, Length=489, Percent_Identity=24.7443762781186, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=24.7443762781186, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71983419, Length=468, Percent_Identity=25.8547008547009, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71983429, Length=468, Percent_Identity=25.8547008547009, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=40.7949790794979, Blast_Score=309, Evalue=8e-85,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=28.8981288981289, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=27.3885350318471, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21358499, Length=477, Percent_Identity=41.5094339622642, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=25.3578732106339, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=25.1533742331288, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=25.1533742331288, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=26.2396694214876, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH2_BACSU (P54533)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   M97391
- PIR:   D69962
- RefSeq:   NP_390286.2
- ProteinModelPortal:   P54533
- SMR:   P54533
- EnsemblBacteria:   EBBACT00000000562
- GeneID:   938669
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24060
- GenoList:   BSU24060
- GeneTree:   EBGT00070000031801
- HOGENOM:   HBG515043
- PhylomeDB:   P54533
- ProtClustDB:   PRK05976
- BioCyc:   BSUB:BSU24060-MONOMER
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 50436; Mature: 50305

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 453-453 BINDING 51-51 BINDING 114-114 BINDING 211-211 BINDING 245-245 BINDING 320-320 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKEKLGGTCLHKGCIPSKALLRSAEVY
CCCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
RTAREADQFGVETAGVSLNFEKVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPS
HHHHHHHHCCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCH
IFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRMLPGLEVDGKSVLTSDEALQMEEL
HHCCCCCEEEEECCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHC
PQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILPTEDLEISKEMESLLKKKGIQFI
CCEEEEEECCEEEHHHHHHHHHCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVLPDTMTKTSDDISIQAEKDGETVTYSAEKMLVSIGRQANIEGIGLENTDIVTENG
ECCCCCCCHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
MISVNESCQTKESHIYAIGDVIGGLQLAHVASHEGIIAVEHFAGLNPHPLDPTLVPKCIY
EEEECCCCCCCCCEEEEHHHHHCCHHEEEHHCCCCEEEEEECCCCCCCCCCCCHHHHHHC
SSPEAASVGLTEDEAKANGHNVKIGKFPFMAIGKALVYGESDGFVKIVADRDTDDILGVH
CCCCCCCCCCCCCHHCCCCCEEEECCCCHHHHCCEEEEECCCCEEEEEECCCCCCEEEEE
MIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAADGKAIHF
ECCCHHHHHHHHCCHHHHHCCCCHHHCCCCCCCCCHHHHHCCHHHHCCCCEECC
>Mature Secondary Structure 
ATEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKEKLGGTCLHKGCIPSKALLRSAEVY
CCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
RTAREADQFGVETAGVSLNFEKVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPS
HHHHHHHHCCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCH
IFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRMLPGLEVDGKSVLTSDEALQMEEL
HHCCCCCEEEEECCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHC
PQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILPTEDLEISKEMESLLKKKGIQFI
CCEEEEEECCEEEHHHHHHHHHCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVLPDTMTKTSDDISIQAEKDGETVTYSAEKMLVSIGRQANIEGIGLENTDIVTENG
ECCCCCCCHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
MISVNESCQTKESHIYAIGDVIGGLQLAHVASHEGIIAVEHFAGLNPHPLDPTLVPKCIY
EEEECCCCCCCCCEEEEHHHHHCCHHEEEHHCCCCEEEEEECCCCCCCCCCCCHHHHHHC
SSPEAASVGLTEDEAKANGHNVKIGKFPFMAIGKALVYGESDGFVKIVADRDTDDILGVH
CCCCCCCCCCCCCHHCCCCCEEEECCCCHHHHCCEEEEECCCCEEEEEECCCCCCEEEEE
MIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAADGKAIHF
ECCCHHHHHHHHCCHHHHHCCCCHHHCCCCCCCCCHHHHHCCHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804