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The map label for this gene is bkdAA

Identifier: 16079461

GI number: 16079461

Start: 2499090

End: 2500082

Strand: Reverse

Name: bkdAA

Synonym: BSU24050

Alternate gene names: 16079461

Gene position: 2500082-2499090 (Counterclockwise)

Preceding gene: 255767526

Following gene: 16079460

Centisome position: 59.31

GC content: 47.73

Gene sequence:

>993_bases
ATGAGTACAAACCGACATCAAGCACTAGGGCTGACTGATCAGGAAGCCGTTGATATGTATAGAACCATGCTGTTAGCAAG
AAAAATCGATGAAAGAATGTGGCTGTTAAACCGTTCTGGCAAAATTCCATTTGTAATCTCTTGTCAAGGACAGGAAGCAG
CACAGGTAGGAGCGGCTTTCGCACTTGACCGTGAAATGGATTATGTATTGCCGTACTACAGAGACATGGGTGTCGTGCTC
GCGTTTGGCATGACAGCAAAGGACTTAATGATGTCCGGGTTTGCAAAAGCAGCAGATCCGAACTCAGGAGGCCGCCAGAT
GCCGGGACATTTCGGACAAAAGAAAAACCGCATTGTGACGGGATCATCTCCGGTTACAACGCAAGTGCCGCACGCAGTCG
GTATTGCGCTTGCGGGACGTATGGAGAAAAAGGATATCGCAGCCTTTGTTACATTCGGGGAAGGGTCTTCAAACCAAGGC
GATTTCCATGAAGGGGCAAACTTTGCCGCTGTCCATAAGCTGCCGGTTATTTTCATGTGTGAAAACAACAAATACGCAAT
CTCAGTGCCTTACGATAAGCAAGTCGCATGTGAGAACATTTCCGACCGTGCCATAGGCTATGGGATGCCTGGCGTAACTG
TGAATGGAAATGATCCGCTGGAAGTTTATCAAGCGGTTAAAGAAGCACGCGAAAGGGCACGCAGAGGAGAAGGCCCGACA
TTAATTGAAACGATTTCTTACCGCCTTACACCACATTCCAGTGATGACGATGACAGCAGCTACAGAGGCCGTGAAGAAGT
AGAGGAAGCGAAAAAAAGTGATCCCCTGCTTACTTATCAAGCTTACTTAAAGGAAACAGGCCTGCTGTCCGATGAGATAG
AACAAACCATGCTGGATGAAATTATGGCAATCGTAAATGAAGCGACGGATGAAGCGGAGAACGCCCCATATGCAGCTCCT
GAGTCAGCGCTTGATTATGTTTATGCGAAGTAG

Upstream 100 bases:

>100_bases
GATTCACCCGCATCCAACGCTTTCTGAAGCAATTGGAGAAGCTGCGCTTGCCGCAGATGGCAAAGCCATTCATTTTTAAA
AGCATAAAGGAGGGGCTTGA

Downstream 100 bases:

>100_bases
GGAGGAAGAACAAATGTCAGTAATGTCATATATTGATGCAATCAATTTGGCGATGAAAGAAGAAATGGAACGAGATTCTC
GCGTTTTCGTCCTTGGGGAA

Product: branched-chain alpha-keto acid dehydrogenase E1 subunit

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSTNRHQALGLTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDREMDYVLPYYRDMGVVL
AFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQG
DFHEGANFAAVHKLPVIFMCENNKYAISVPYDKQVACENISDRAIGYGMPGVTVNGNDPLEVYQAVKEARERARRGEGPT
LIETISYRLTPHSSDDDDSSYRGREEVEEAKKSDPLLTYQAYLKETGLLSDEIEQTMLDEIMAIVNEATDEAENAPYAAP
ESALDYVYAK

Sequences:

>Translated_330_residues
MSTNRHQALGLTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDREMDYVLPYYRDMGVVL
AFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQG
DFHEGANFAAVHKLPVIFMCENNKYAISVPYDKQVACENISDRAIGYGMPGVTVNGNDPLEVYQAVKEARERARRGEGPT
LIETISYRLTPHSSDDDDSSYRGREEVEEAKKSDPLLTYQAYLKETGLLSDEIEQTMLDEIMAIVNEATDEAENAPYAAP
ESALDYVYAK
>Mature_329_residues
STNRHQALGLTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDREMDYVLPYYRDMGVVLA
FGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQGD
FHEGANFAAVHKLPVIFMCENNKYAISVPYDKQVACENISDRAIGYGMPGVTVNGNDPLEVYQAVKEARERARRGEGPTL
IETISYRLTPHSSDDDDSSYRGREEVEEAKKSDPLLTYQAYLKETGLLSDEIEQTMLDEIMAIVNEATDEAENAPYAAPE
SALDYVYAK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family

Homologues:

Organism=Homo sapiens, GI11386135, Length=319, Percent_Identity=37.6175548589342, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI258645172, Length=319, Percent_Identity=37.6175548589342, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI4885543, Length=319, Percent_Identity=32.9153605015674, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4505685, Length=317, Percent_Identity=31.8611987381703, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI291084742, Length=317, Percent_Identity=31.8611987381703, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI291084744, Length=324, Percent_Identity=31.1728395061728, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI291084757, Length=168, Percent_Identity=36.9047619047619, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI86563355, Length=300, Percent_Identity=39, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI86563357, Length=300, Percent_Identity=39, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17536047, Length=323, Percent_Identity=30.030959752322, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564172, Length=323, Percent_Identity=30.030959752322, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=31.0344827586207, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21355903, Length=286, Percent_Identity=34.2657342657343, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24639740, Length=312, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24639744, Length=312, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28571106, Length=312, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24639748, Length=310, Percent_Identity=30.9677419354839, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24639746, Length=272, Percent_Identity=31.6176470588235, Blast_Score=121, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ODBA_BACSU (P37940)

Other databases:

- EMBL:   M97391
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   C69593
- RefSeq:   NP_390285.1
- ProteinModelPortal:   P37940
- SMR:   P37940
- EnsemblBacteria:   EBBACT00000002078
- GeneID:   938674
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24050
- NMPDR:   fig|224308.1.peg.2409
- GenoList:   BSU24050
- GeneTree:   EBGT00050000001071
- HOGENOM:   HBG753263
- OMA:   DYVLPYY
- ProtClustDB:   CLSK2752445
- BioCyc:   BSUB:BSU24050-MONOMER
- BioCyc:   MetaCyc:MONOMER-11683
- BRENDA:   1.2.4.4
- InterPro:   IPR001017

Pfam domain/function: PF00676 E1_dh

EC number: =1.2.4.4

Molecular weight: Translated: 36334; Mature: 36203

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 73-73 BINDING 123-123 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNRHQALGLTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
ALDREMDYVLPYYRDMGVVLAFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVT
HHCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
GSSPVTTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQGDFHEGANFAAVHKLPVIFMC
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHCCCCEEEEECCCEEEEE
ENNKYAISVPYDKQVACENISDRAIGYGMPGVTVNGNDPLEVYQAVKEARERARRGEGPT
CCCCEEEECCCCCCHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCE
LIETISYRLTPHSSDDDDSSYRGREEVEEAKKSDPLLTYQAYLKETGLLSDEIEQTMLDE
EEHEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHH
IMAIVNEATDEAENAPYAAPESALDYVYAK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
STNRHQALGLTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
ALDREMDYVLPYYRDMGVVLAFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVT
HHCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
GSSPVTTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQGDFHEGANFAAVHKLPVIFMC
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHCCCCEEEEECCCEEEEE
ENNKYAISVPYDKQVACENISDRAIGYGMPGVTVNGNDPLEVYQAVKEARERARRGEGPT
CCCCEEEECCCCCCHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCE
LIETISYRLTPHSSDDDDSSYRGREEVEEAKKSDPLLTYQAYLKETGLLSDEIEQTMLDE
EEHEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHH
IMAIVNEATDEAENAPYAAPESALDYVYAK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377