Click here to switch to the map view.

The map label for this gene is bmrU

Identifier: 255767524

GI number: 255767524

Start: 2493662

End: 2494555

Strand: Direct

Name: bmrU

Synonym: BSU24000

Alternate gene names: 255767524

Gene position: 2493662-2494555 (Clockwise)

Preceding gene: 255767519

Following gene: 255767525

Centisome position: 59.15

GC content: 44.97

Gene sequence:

>894_bases
ATGAGCCACCGGAAAGCGTTACTGATTCATAACGGAAATGCCGGCAACAAAAATATAGAAAAAGCACTTGGTGCTGTGGT
GCCGGTTTTATCTCACCATTTAGATGAGGTGATTATTAAACAGACAAAGAAAAAGGATGATGCGTATCATTTTTGCCGAT
CCATTGATGATTCTGTCGACACCGTCTTCATTCTCGGCGGCGACGGCACGATCCATCAATGCATAAATGCTATCAGCGCT
CTAGAAAGAAAACCCGCTGTCGGAATTTTGCCCGGCGGCACGTGCAACGACTTTTCAAGAGTGCTTGGCATCCCTCAAAA
TCTTGCAAAGGCCGCGGAAGCACTTATGGCGGGAAAAAAAACCAGCGTAGACGTATGTCAGATGAATGACCGTTATTTTT
TGAATTTCTGGGGGATCGGCTTAATTGCAGAAACGTCGAATCAAATCAACGAAACGGAAAAAGCATTATTGGGAAAAATC
AGCTATTTTACAAGTGCCTTGCGAACCGTCTCATCTGCTGCATCGTTTCCGATGACATTAAAAATTGATGGAGAAGAAAT
TAAAGAAGAAGCCGTTATGCTGCTTGTGATGAATGGCCAATACATTGGAACGAATCGAATTCCTCTGCCCGATGCCAGTA
TTGAAGACGGCTTACTTGATGTTCTCATATGCCGCAATACCAACCTCACCGCGCTGCGGGAGCTGATGAGCATGGAACAA
GGCTCAATTGATCGTTTTACTGGAGAGTTGTCCTATGTCCAGGCTTCCCGTATCGAGATTGAAACAGACACTGCCAAAAA
GGCGGATATGGATGGAGAAGTTTATACACATACACCCGCAGTCATTCAGGTCCTGCCACAGCATATCGACATGCTTGTTC
CCGCAAATGAATAA

Upstream 100 bases:

>100_bases
AAATGACTTTGCTTCGCCGTGCACTTTTTTTGCTCTTCGTTTACTGCTTACAGAAAAAGGGGATTATATAACCAGAAGTA
AAATAAAGGAGCAGTTGTAT

Downstream 100 bases:

>100_bases
CTGTCATAAGGCTTTAGAAAGATTTTGCAAATCCGTTGACTCTCCCCTAGGAGGAGGTCTTACAGTATAAGGGATACACA
CTTGAAAAAGGGGAAGTCAT

Product: diacylglycerol kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSHRKALLIHNGNAGNKNIEKALGAVVPVLSHHLDEVIIKQTKKKDDAYHFCRSIDDSVDTVFILGGDGTIHQCINAISA
LERKPAVGILPGGTCNDFSRVLGIPQNLAKAAEALMAGKKTSVDVCQMNDRYFLNFWGIGLIAETSNQINETEKALLGKI
SYFTSALRTVSSAASFPMTLKIDGEEIKEEAVMLLVMNGQYIGTNRIPLPDASIEDGLLDVLICRNTNLTALRELMSMEQ
GSIDRFTGELSYVQASRIEIETDTAKKADMDGEVYTHTPAVIQVLPQHIDMLVPANE

Sequences:

>Translated_297_residues
MSHRKALLIHNGNAGNKNIEKALGAVVPVLSHHLDEVIIKQTKKKDDAYHFCRSIDDSVDTVFILGGDGTIHQCINAISA
LERKPAVGILPGGTCNDFSRVLGIPQNLAKAAEALMAGKKTSVDVCQMNDRYFLNFWGIGLIAETSNQINETEKALLGKI
SYFTSALRTVSSAASFPMTLKIDGEEIKEEAVMLLVMNGQYIGTNRIPLPDASIEDGLLDVLICRNTNLTALRELMSMEQ
GSIDRFTGELSYVQASRIEIETDTAKKADMDGEVYTHTPAVIQVLPQHIDMLVPANE
>Mature_296_residues
SHRKALLIHNGNAGNKNIEKALGAVVPVLSHHLDEVIIKQTKKKDDAYHFCRSIDDSVDTVFILGGDGTIHQCINAISAL
ERKPAVGILPGGTCNDFSRVLGIPQNLAKAAEALMAGKKTSVDVCQMNDRYFLNFWGIGLIAETSNQINETEKALLGKIS
YFTSALRTVSSAASFPMTLKIDGEEIKEEAVMLLVMNGQYIGTNRIPLPDASIEDGLLDVLICRNTNLTALRELMSMEQG
SIDRFTGELSYVQASRIEIETDTAKKADMDGEVYTHTPAVIQVLPQHIDMLVPANE

Specific function: May catalyze the ATP-dependent phosphorylation of lipids other than diacylglycerol (DAG). In fact, is not able to exhibit diacylglycerol kinase activity in vitro

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain

Homologues:

Organism=Escherichia coli, GI1788402, Length=265, Percent_Identity=29.0566037735849, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BMRU_BACSU (P39074)

Other databases:

- EMBL:   L25604
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   F69595
- RefSeq:   NP_390280.2
- ProteinModelPortal:   P39074
- SMR:   P39074
- EnsemblBacteria:   EBBACT00000002167
- GeneID:   938679
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24000
- NMPDR:   fig|224308.1.peg.2404
- GenoList:   BSU24000
- GeneTree:   EBGT00050000001540
- HOGENOM:   HBG338166
- PhylomeDB:   P39074
- ProtClustDB:   CLSK887541
- BioCyc:   BSUB:BSU24000-MONOMER
- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206
- Gene3D:   G3DSA:3.40.50.10330
- SMART:   SM00046
- TIGRFAMs:   TIGR00147

Pfam domain/function: PF00781 DAGK_cat

EC number: NA

Molecular weight: Translated: 32442; Mature: 32310

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50146 DAGK

Important sites: ACT_SITE 273-273 BINDING 42-42 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRKALLIHNGNAGNKNIEKALGAVVPVLSHHLDEVIIKQTKKKDDAYHFCRSIDDSVD
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TVFILGGDGTIHQCINAISALERKPAVGILPGGTCNDFSRVLGIPQNLAKAAEALMAGKK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCC
TSVDVCQMNDRYFLNFWGIGLIAETSNQINETEKALLGKISYFTSALRTVSSAASFPMTL
CCCEEEECCCCEEEEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KIDGEEIKEEAVMLLVMNGQYIGTNRIPLPDASIEDGLLDVLICRNTNLTALRELMSMEQ
EECHHHHHCCEEEEEEECCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
GSIDRFTGELSYVQASRIEIETDTAKKADMDGEVYTHTPAVIQVLPQHIDMLVPANE
CCHHHHHCCHHEEEEEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHEEECCCC
>Mature Secondary Structure 
SHRKALLIHNGNAGNKNIEKALGAVVPVLSHHLDEVIIKQTKKKDDAYHFCRSIDDSVD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
TVFILGGDGTIHQCINAISALERKPAVGILPGGTCNDFSRVLGIPQNLAKAAEALMAGKK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCC
TSVDVCQMNDRYFLNFWGIGLIAETSNQINETEKALLGKISYFTSALRTVSSAASFPMTL
CCCEEEECCCCEEEEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KIDGEEIKEEAVMLLVMNGQYIGTNRIPLPDASIEDGLLDVLICRNTNLTALRELMSMEQ
EECHHHHHCCEEEEEEECCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
GSIDRFTGELSYVQASRIEIETDTAKKADMDGEVYTHTPAVIQVLPQHIDMLVPANE
CCHHHHHCCHHEEEEEEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961792; 8969508; 9384377