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The map label for this gene is bmr

Identifier: 255767525

GI number: 255767525

Start: 2494656

End: 2495825

Strand: Direct

Name: bmr

Synonym: BSU24010

Alternate gene names: 255767525

Gene position: 2494656-2495825 (Clockwise)

Preceding gene: 255767524

Following gene: 50812267

Centisome position: 59.18

GC content: 44.53

Gene sequence:

>1170_bases
ATGGAGAAGAAAAATATTACCTTAACTATATTATTAACCAATTTATTTATTGCTTTTTTGGGGATCGGGCTTGTGATTCC
AGTAACGCCGACCATTATGAATGAATTGCATTTATCGGGGACCGCGGTCGGCTATATGGTTGCCTGCTTCGCTATTACAC
AGCTCATTGTCTCACCAATAGCCGGACGATGGGTTGATCGCTTCGGGCGCAAGATCATGATCGTAATCGGCCTGTTGTTC
TTTAGTGTGTCGGAGTTTTTGTTCGGCATTGGAAAAACAGTTGAGATGTTATTTATCTCCCGTATGCTGGGCGGTATCAG
CGCAGCCTTCATTATGCCCGGGGTCACGGCTTTTATTGCAGATATCACGACCATTAAAACACGGCCAAAAGCGCTCGGTT
ATATGTCAGCCGCTATTTCAACAGGATTTATTATCGGCCCCGGCATCGGGGGATTTTTAGCAGAAGTCCATTCCCGGCTG
CCTTTTTTCTTTGCGGCAGCTTTTGCACTGTTAGCAGCCATTTTATCAATCCTCACGCTGCGCGAGCCGGAACGAAACCC
TGAAAATCAGGAAATAAAAGGACAGAAGACAGGCTTTAAACGAATTTTTGCCCCCATGTATTTCATAGCTTTTCTCATTA
TCTTAATTTCGTCTTTTGGTTTAGCATCATTTGAATCTTTATTTGCATTATTCGTGGATCATAAATTCGGATTTACGGCC
AGCGACATTGCCATTATGATTACAGGAGGAGCGATTGTTGGCGCCATTACGCAAGTCGTCTTATTCGACCGCTTCACAAG
ATGGTTTGGCGAAATTCATTTAATTCGGTACAGCTTAATTCTCTCGACGAGTCTGGTATTCTTGCTGACAACGGTACATT
CATATGTTGCGATTCTGCTGGTGACAGTCACCGTATTTGTCGGATTTGATCTCATGCGGCCTGCGGTAACGACTTACCTG
TCAAAGATTGCGGGAAATGAACAGGGGTTTGCCGGCGGTATGAATTCAATGTTTACAAGTATCGGCAATGTATTCGGGCC
TATTATCGGCGGAATGCTGTTCGATATAGATGTAAACTATCCTTTCTACTTTGCAACGGTCACCTTAGCCATAGGGATTG
CACTGACCATTGCTTGGAAAGCGCCTGCACATCTTAAAGCCAGCACGTGA

Upstream 100 bases:

>100_bases
CTGTCATAAGGCTTTAGAAAGATTTTGCAAATCCGTTGACTCTCCCCTAGGAGGAGGTCTTACAGTATAAGGGATACACA
CTTGAAAAAGGGGAAGTCAT

Downstream 100 bases:

>100_bases
TAAGAAGCGCATTCTTTGTGTACTGCAAAGAATGCGCTTCTTCTTTATACTGATGATAGAAGGAGTGATGGCATGAAGGA
ATCGTATTACTCAATTGGGG

Product: multidrug-efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 1

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MEKKNITLTILLTNLFIAFLGIGLVIPVTPTIMNELHLSGTAVGYMVACFAITQLIVSPIAGRWVDRFGRKIMIVIGLLF
FSVSEFLFGIGKTVEMLFISRMLGGISAAFIMPGVTAFIADITTIKTRPKALGYMSAAISTGFIIGPGIGGFLAEVHSRL
PFFFAAAFALLAAILSILTLREPERNPENQEIKGQKTGFKRIFAPMYFIAFLIILISSFGLASFESLFALFVDHKFGFTA
SDIAIMITGGAIVGAITQVVLFDRFTRWFGEIHLIRYSLILSTSLVFLLTTVHSYVAILLVTVTVFVGFDLMRPAVTTYL
SKIAGNEQGFAGGMNSMFTSIGNVFGPIIGGMLFDIDVNYPFYFATVTLAIGIALTIAWKAPAHLKAST

Sequences:

>Translated_389_residues
MEKKNITLTILLTNLFIAFLGIGLVIPVTPTIMNELHLSGTAVGYMVACFAITQLIVSPIAGRWVDRFGRKIMIVIGLLF
FSVSEFLFGIGKTVEMLFISRMLGGISAAFIMPGVTAFIADITTIKTRPKALGYMSAAISTGFIIGPGIGGFLAEVHSRL
PFFFAAAFALLAAILSILTLREPERNPENQEIKGQKTGFKRIFAPMYFIAFLIILISSFGLASFESLFALFVDHKFGFTA
SDIAIMITGGAIVGAITQVVLFDRFTRWFGEIHLIRYSLILSTSLVFLLTTVHSYVAILLVTVTVFVGFDLMRPAVTTYL
SKIAGNEQGFAGGMNSMFTSIGNVFGPIIGGMLFDIDVNYPFYFATVTLAIGIALTIAWKAPAHLKAST
>Mature_389_residues
MEKKNITLTILLTNLFIAFLGIGLVIPVTPTIMNELHLSGTAVGYMVACFAITQLIVSPIAGRWVDRFGRKIMIVIGLLF
FSVSEFLFGIGKTVEMLFISRMLGGISAAFIMPGVTAFIADITTIKTRPKALGYMSAAISTGFIIGPGIGGFLAEVHSRL
PFFFAAAFALLAAILSILTLREPERNPENQEIKGQKTGFKRIFAPMYFIAFLIILISSFGLASFESLFALFVDHKFGFTA
SDIAIMITGGAIVGAITQVVLFDRFTRWFGEIHLIRYSLILSTSLVFLLTTVHSYVAILLVTVTVFVGFDLMRPAVTTYL
SKIAGNEQGFAGGMNSMFTSIGNVFGPIIGGMLFDIDVNYPFYFATVTLAIGIALTIAWKAPAHLKAST

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Homo sapiens, GI217416366, Length=393, Percent_Identity=21.3740458015267, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI118582257, Length=143, Percent_Identity=35.6643356643357, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787291, Length=367, Percent_Identity=26.4305177111717, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71999703, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62471713, Length=242, Percent_Identity=28.099173553719, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI62471717, Length=242, Percent_Identity=28.099173553719, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI62471715, Length=242, Percent_Identity=28.099173553719, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17738085, Length=351, Percent_Identity=26.2108262108262, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17137160, Length=217, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BMR1_BACSU (P33449)

Other databases:

- EMBL:   M33768
- EMBL:   L25604
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A39705
- RefSeq:   NP_390281.2
- ProteinModelPortal:   P33449
- SMR:   P33449
- EnsemblBacteria:   EBBACT00000002791
- GeneID:   938680
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24010
- NMPDR:   fig|224308.1.peg.2405
- GenoList:   BSU24010
- GeneTree:   EBGT00070000031981
- HOGENOM:   HBG710362
- ProtClustDB:   CLSK887542
- BioCyc:   BSUB:BSU24010-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- PRINTS:   PR01035

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 42233; Mature: 42233

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10b9b3bc)-; HASH(0x104ed2b4)-; HASH(0x1019f970)-; HASH(0xecee84c)-; HASH(0xd4b485c)-; HASH(0x10c93220)-; HASH(0x10b95ca4)-; HASH(0xfd3a260)-; HASH(0x10b88470)-; HASH(0x10ba3b98)-; HASH(0x10351c10)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKNITLTILLTNLFIAFLGIGLVIPVTPTIMNELHLSGTAVGYMVACFAITQLIVSPI
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGRWVDRFGRKIMIVIGLLFFSVSEFLFGIGKTVEMLFISRMLGGISAAFIMPGVTAFIA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DITTIKTRPKALGYMSAAISTGFIIGPGIGGFLAEVHSRLPFFFAAAFALLAAILSILTL
HHHHHCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
REPERNPENQEIKGQKTGFKRIFAPMYFIAFLIILISSFGLASFESLFALFVDHKFGFTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
SDIAIMITGGAIVGAITQVVLFDRFTRWFGEIHLIRYSLILSTSLVFLLTTVHSYVAILL
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVTVFVGFDLMRPAVTTYLSKIAGNEQGFAGGMNSMFTSIGNVFGPIIGGMLFDIDVNY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCC
PFYFATVTLAIGIALTIAWKAPAHLKAST
CHHHHHHHHHHHHHHHEEECCCCCCCCCH
>Mature Secondary Structure
MEKKNITLTILLTNLFIAFLGIGLVIPVTPTIMNELHLSGTAVGYMVACFAITQLIVSPI
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGRWVDRFGRKIMIVIGLLFFSVSEFLFGIGKTVEMLFISRMLGGISAAFIMPGVTAFIA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DITTIKTRPKALGYMSAAISTGFIIGPGIGGFLAEVHSRLPFFFAAAFALLAAILSILTL
HHHHHCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
REPERNPENQEIKGQKTGFKRIFAPMYFIAFLIILISSFGLASFESLFALFVDHKFGFTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
SDIAIMITGGAIVGAITQVVLFDRFTRWFGEIHLIRYSLILSTSLVFLLTTVHSYVAILL
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVTVFVGFDLMRPAVTTYLSKIAGNEQGFAGGMNSMFTSIGNVFGPIIGGMLFDIDVNY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCC
PFYFATVTLAIGIALTIAWKAPAHLKAST
CHHHHHHHHHHHHHHHEEECCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1675788; 7961792; 8969508; 9384377