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The map label for this gene is spoVAF

Identifier: 255767504

GI number: 255767504

Start: 2438372

End: 2439853

Strand: Reverse

Name: spoVAF

Synonym: BSU23390

Alternate gene names: 255767504

Gene position: 2439853-2438372 (Counterclockwise)

Preceding gene: 255767505

Following gene: 16079395

Centisome position: 57.88

GC content: 44.74

Gene sequence:

>1482_bases
ATGCCGGACCACAAGGAAGAGAAAATTCGGGTTTATCGGAATCCAGCTAAAAATGAAGAGTATTTCAAAAACCGTGTTGG
GATGGGGACGAGCTATGATGTAGGTGTTCGCAAGCTCACGATTTTAGATAAAGAGATCCAGCTTTACTATCTAAATGGAT
TATGCGATACAGCCTATATTATTCATTTAATGAGAGAGCTTGTTGCCATTAACAATCGGAAAGAAGACCCTGATGAGCTG
GTCGACATCGTCGAAAACAGGCTGCTTAACGCCCAGGTCGAAAAAGTAAAAACCTTGGATGAAACCACCGACCAAGTGCT
GTCCGGGCTCGTCGCTGTCATTGTTGAAGGTGCAGGCTTCGCATTTATAATTGATGTCAGAAGCTATCCGGGCAGAAACC
CGGAAGAACCTGATACGGAAAAGGTGGTCAGGGGAGCCAGAGACGGATTTGTTGAAAATATCGTCGTCAATACGGCCCTT
CTCAGAAGGCGGATCAGAGACGAACGCCTTCGAGTCAAGATGACTAAAGTTGGTGAGCGGTCAAAGACGGATTTAAGCAT
TTGTTATATAGAAGATATAGCTGATCCGGATCTGGTTGAGATCGTAGAAAAAGAAATTGCATCCATTGATGTCGATGGAT
TAACAATGGCAGACAAAACTGTCGAGGAATTCATTGTTAATCAAAGCTATAATCCTTTTCCGCTCGTGCGCTATACAGAA
AGACCGGATGTGGCCGCAAATCATGTTCTAGAAGGCCATGTTATCATTATAGTGGACACATCGCCGAGTGTGATCATTAC
CCCGACGACTTTATTTCACCATGTGCAGCATGCTGAAGAATATAGGCAGGCGCCTTCAGTCGGGACCTTTTTAAGATGGG
TAAGATTTTTCGGTATTCTTGCTTCTACTTTATTTCTTCCGATCTGGTTTCTCTTTGTGCTTCAGCCTGATCTGCTCCCG
GACAACATGAAATTTATCGGTTTAAATAAAGATACGCATATCCCGATCATCCTGCAGATATTTTTAGCTGACTTAGGAAT
CGAGTTTCTCAGAATGGCTGCCATTCACACACCGACGGCTTTATCGACAGCAATGGGTTTAATTGCCGCTGTTCTGATCG
GGCAGATTGCGATTGAGGTCGGCCTTTTTTCTCCTGAGGTGATTCTGTACGTCTCACTTGCGGCGATTGGAACCTTTACG
ACGCCTAGTTATGAATTAAGCTTGGCAAACAAAATGAGCCGTCTTGTCCTCATGATACTCGTTGCTTTATTTCATATAAA
AGGGCTCGTCATCGGATTTACAGTGCTAATTATTGCCATGGCAAGCATCAAATCCCTTCAGACACCGTACTTATGGCCGC
TGATTCCCTTTAACGGAAAGGCGCTGTGGCAGGTTCTTGTCCGCACAGCGAAACCTGGCGCAAAAGTAAGACCCAGCATC
GTTCACCCCAAAAATCGCTTAAGGCAGCCTACCAATTCATAA

Upstream 100 bases:

>100_bases
ATCGGGGATATCGGCAAAATGGGCAGAAAAGACGATATCTCAAAAGGGTCGCCAATCACCATGAAAGCGGTCGAGTTTAT
TTTAGAAAGGAGCGGGTATC

Downstream 100 bases:

>100_bases
TCGCATTGAAACTGACTGAAGAGTATGATAATGTATGTCTTAATTATGAAGGGACAGTCCATAAAACTGTCCTTTCATCC
ATAAGCGGAGAAAGAGGGAA

Product: stage V sporulation protein AF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MPDHKEEKIRVYRNPAKNEEYFKNRVGMGTSYDVGVRKLTILDKEIQLYYLNGLCDTAYIIHLMRELVAINNRKEDPDEL
VDIVENRLLNAQVEKVKTLDETTDQVLSGLVAVIVEGAGFAFIIDVRSYPGRNPEEPDTEKVVRGARDGFVENIVVNTAL
LRRRIRDERLRVKMTKVGERSKTDLSICYIEDIADPDLVEIVEKEIASIDVDGLTMADKTVEEFIVNQSYNPFPLVRYTE
RPDVAANHVLEGHVIIIVDTSPSVIITPTTLFHHVQHAEEYRQAPSVGTFLRWVRFFGILASTLFLPIWFLFVLQPDLLP
DNMKFIGLNKDTHIPIILQIFLADLGIEFLRMAAIHTPTALSTAMGLIAAVLIGQIAIEVGLFSPEVILYVSLAAIGTFT
TPSYELSLANKMSRLVLMILVALFHIKGLVIGFTVLIIAMASIKSLQTPYLWPLIPFNGKALWQVLVRTAKPGAKVRPSI
VHPKNRLRQPTNS

Sequences:

>Translated_493_residues
MPDHKEEKIRVYRNPAKNEEYFKNRVGMGTSYDVGVRKLTILDKEIQLYYLNGLCDTAYIIHLMRELVAINNRKEDPDEL
VDIVENRLLNAQVEKVKTLDETTDQVLSGLVAVIVEGAGFAFIIDVRSYPGRNPEEPDTEKVVRGARDGFVENIVVNTAL
LRRRIRDERLRVKMTKVGERSKTDLSICYIEDIADPDLVEIVEKEIASIDVDGLTMADKTVEEFIVNQSYNPFPLVRYTE
RPDVAANHVLEGHVIIIVDTSPSVIITPTTLFHHVQHAEEYRQAPSVGTFLRWVRFFGILASTLFLPIWFLFVLQPDLLP
DNMKFIGLNKDTHIPIILQIFLADLGIEFLRMAAIHTPTALSTAMGLIAAVLIGQIAIEVGLFSPEVILYVSLAAIGTFT
TPSYELSLANKMSRLVLMILVALFHIKGLVIGFTVLIIAMASIKSLQTPYLWPLIPFNGKALWQVLVRTAKPGAKVRPSI
VHPKNRLRQPTNS
>Mature_492_residues
PDHKEEKIRVYRNPAKNEEYFKNRVGMGTSYDVGVRKLTILDKEIQLYYLNGLCDTAYIIHLMRELVAINNRKEDPDELV
DIVENRLLNAQVEKVKTLDETTDQVLSGLVAVIVEGAGFAFIIDVRSYPGRNPEEPDTEKVVRGARDGFVENIVVNTALL
RRRIRDERLRVKMTKVGERSKTDLSICYIEDIADPDLVEIVEKEIASIDVDGLTMADKTVEEFIVNQSYNPFPLVRYTER
PDVAANHVLEGHVIIIVDTSPSVIITPTTLFHHVQHAEEYRQAPSVGTFLRWVRFFGILASTLFLPIWFLFVLQPDLLPD
NMKFIGLNKDTHIPIILQIFLADLGIEFLRMAAIHTPTALSTAMGLIAAVLIGQIAIEVGLFSPEVILYVSLAAIGTFTT
PSYELSLANKMSRLVLMILVALFHIKGLVIGFTVLIIAMASIKSLQTPYLWPLIPFNGKALWQVLVRTAKPGAKVRPSIV
HPKNRLRQPTNS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gerABKA family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SP5AF_BACSU (P31845)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   L09228
- EMBL:   D90189
- EMBL:   M15349
- PIR:   A69715
- RefSeq:   NP_390220.2
- EnsemblBacteria:   EBBACT00000003896
- GeneID:   938936
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23390
- GenoList:   BSU23390
- GeneTree:   EBGT00070000032094
- HOGENOM:   HBG343112
- ProtClustDB:   CLSK873021
- BioCyc:   BSUB:BSU23390-MONOMER
- InterPro:   IPR004995

Pfam domain/function: PF03323 GerA

EC number: NA

Molecular weight: Translated: 55474; Mature: 55342

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1020d864)-; HASH(0x1076c708)-; HASH(0x102d91c0)-; HASH(0xff16c48)-; HASH(0x100abba8)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDHKEEKIRVYRNPAKNEEYFKNRVGMGTSYDVGVRKLTILDKEIQLYYLNGLCDTAYI
CCCCCCCHHHEECCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHH
IHLMRELVAINNRKEDPDELVDIVENRLLNAQVEKVKTLDETTDQVLSGLVAVIVEGAGF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AFIIDVRSYPGRNPEEPDTEKVVRGARDGFVENIVVNTALLRRRIRDERLRVKMTKVGER
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCC
SKTDLSICYIEDIADPDLVEIVEKEIASIDVDGLTMADKTVEEFIVNQSYNPFPLVRYTE
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECC
RPDVAANHVLEGHVIIIVDTSPSVIITPTTLFHHVQHAEEYRQAPSVGTFLRWVRFFGIL
CCCHHHHHEECCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASTLFLPIWFLFVLQPDLLPDNMKFIGLNKDTHIPIILQIFLADLGIEFLRMAAIHTPTA
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
LSTAMGLIAAVLIGQIAIEVGLFSPEVILYVSLAAIGTFTTPSYELSLANKMSRLVLMIL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHH
VALFHIKGLVIGFTVLIIAMASIKSLQTPYLWPLIPFNGKALWQVLVRTAKPGAKVRPSI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
VHPKNRLRQPTNS
CCCHHHCCCCCCH
>Mature Secondary Structure 
PDHKEEKIRVYRNPAKNEEYFKNRVGMGTSYDVGVRKLTILDKEIQLYYLNGLCDTAYI
CCCCCCHHHEECCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHH
IHLMRELVAINNRKEDPDELVDIVENRLLNAQVEKVKTLDETTDQVLSGLVAVIVEGAGF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AFIIDVRSYPGRNPEEPDTEKVVRGARDGFVENIVVNTALLRRRIRDERLRVKMTKVGER
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCC
SKTDLSICYIEDIADPDLVEIVEKEIASIDVDGLTMADKTVEEFIVNQSYNPFPLVRYTE
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECC
RPDVAANHVLEGHVIIIVDTSPSVIITPTTLFHHVQHAEEYRQAPSVGTFLRWVRFFGIL
CCCHHHHHEECCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASTLFLPIWFLFVLQPDLLPDNMKFIGLNKDTHIPIILQIFLADLGIEFLRMAAIHTPTA
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
LSTAMGLIAAVLIGQIAIEVGLFSPEVILYVSLAAIGTFTTPSYELSLANKMSRLVLMIL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHH
VALFHIKGLVIGFTVLIIAMASIKSLQTPYLWPLIPFNGKALWQVLVRTAKPGAKVRPSI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
VHPKNRLRQPTNS
CCCHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 7934829; 1368705; 3926949