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The map label for this gene is odhB

Identifier: 255767452

GI number: 255767452

Start: 2107505

End: 2108758

Strand: Reverse

Name: odhB

Synonym: BSU19360

Alternate gene names: 255767452

Gene position: 2108758-2107505 (Counterclockwise)

Preceding gene: 255767453

Following gene: 16078994

Centisome position: 50.02

GC content: 45.06

Gene sequence:

>1254_bases
ATGGCGGAAATTAAGGTACCTGAATTAGCAGAATCAATCTCAGAAGGAACAATAGCCCAATGGTTAAAGCAGCCTGGTGA
CTATGTAGAACAGGGTGAATATCTGCTTGAACTAGAAACGGATAAAGTGAATGTTGAATTGACAGCAGAAGAATCGGGTG
TACTTCAAGAGGTATTGAAAGATTCGGGTGATACCGTCCAGGTCGGAGAAATTATCGGTACGATTTCAGAAGGCGCGGGT
GAAAGTTCTGCCCCTGCTCCTACAGAGAAAACAGAAAGCAAGGAAAGCGTAAAAGAAGAGAAACAGGCTGAACCAGCTGC
ACAAGAGGTGAGCGAGGAAGCACAATCTGAAGCAAAATCAAGAACGATCGCTTCTCCGTCGGCCCGTAAGCTTGCGAGAG
AAAAAGGAATTGACCTGTCTCAAGTTCCAACTGGAGATCCGCTTGGAAGAGTGCGCAAGCAGGATGTCGAAGCGTACGAA
AAACCGGCATCAAAACCGGCTCCTCAGCAAAAGCAGCAGCCTCAGGCTCAAAAAGCACAGCAAAGCTTTGACAAACCTGT
TGAAGTGCAAAAAATGTCACGCCGCAGACAAACGATTGCAAAACGCCTTGTAGAGGTACAGCAAACATCAGCGATGCTGA
CTACATTTAATGAAGTGGACATGACGGCTGTCATGAATCTCAGAAAACGCCGCAAAGATCAATTTTTTGAGCAAAATGAA
GTGAAGCTCGGCTTTATGTCTTTCTTCACGAAAGCGGTCGTGGCTGCATTGAAAAAATATCCGCTGTTGAATGCAGAAAT
TCAAGGCGATGAGTTGATCGTTAAAAAATTCTACGATATCGGAATCGCTGTTGCTGCTGATGAAGGTCTTGTCGTTCCGG
TTGTACGGGATGCGGATCGCCTGACATTTGCAGGAATCGAAAAAGAGATCGGCGAGCTTGCGAAAAAAGCAAGAAACAAT
AAATTAACCCTTAGCGAGCTTCAGGGAGGCTCCTTCACGATTACAAACGGAGGGACTTTCGGTTCATTGATGTCAACTCC
AATTTTAAACAGCCCGCAAGTCGGTATACTGGGCATGCATAAGATTCAGCTGCGCCCTGTAGCCATTGATGAAGAGCGTT
TCGAAAACCGTCCGATGATGTATATCGCTTTATCTTATGATCACCGAATTGTAGACGGTAAAGAAGCGGTTGGTTTCCTC
GTGACAATCAAAAATTTACTGGAAGATCCTGAACAGCTTTTATTAGAAGGATAA

Upstream 100 bases:

>100_bases
ATCCAGCCCTGCAGAGGGAGATCCGACAGTTCATAAAAAAGAACAGGAACGTATTGTATCTGATAGCTTGACTCGCAAAA
ACTAAGGGGGAAATGAAAAA

Downstream 100 bases:

>100_bases
TAAAAAAGGGTACATCACGATAAAGTGATGTACCCTTTTTGATGCATATTTAAAGTGATAGATTGCTGCTTTTGGATCCA
GCTTGCTTCTTTTTCACTTT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLQEVLKDSGDTVQVGEIIGTISEGAG
ESSAPAPTEKTESKESVKEEKQAEPAAQEVSEEAQSEAKSRTIASPSARKLAREKGIDLSQVPTGDPLGRVRKQDVEAYE
KPASKPAPQQKQQPQAQKAQQSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFFEQNE
VKLGFMSFFTKAVVAALKKYPLLNAEIQGDELIVKKFYDIGIAVAADEGLVVPVVRDADRLTFAGIEKEIGELAKKARNN
KLTLSELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIVDGKEAVGFL
VTIKNLLEDPEQLLLEG

Sequences:

>Translated_417_residues
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLQEVLKDSGDTVQVGEIIGTISEGAG
ESSAPAPTEKTESKESVKEEKQAEPAAQEVSEEAQSEAKSRTIASPSARKLAREKGIDLSQVPTGDPLGRVRKQDVEAYE
KPASKPAPQQKQQPQAQKAQQSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFFEQNE
VKLGFMSFFTKAVVAALKKYPLLNAEIQGDELIVKKFYDIGIAVAADEGLVVPVVRDADRLTFAGIEKEIGELAKKARNN
KLTLSELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIVDGKEAVGFL
VTIKNLLEDPEQLLLEG
>Mature_416_residues
AEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLQEVLKDSGDTVQVGEIIGTISEGAGE
SSAPAPTEKTESKESVKEEKQAEPAAQEVSEEAQSEAKSRTIASPSARKLAREKGIDLSQVPTGDPLGRVRKQDVEAYEK
PASKPAPQQKQQPQAQKAQQSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFFEQNEV
KLGFMSFFTKAVVAALKKYPLLNAEIQGDELIVKKFYDIGIAVAADEGLVVPVVRDADRLTFAGIEKEIGELAKKARNNK
LTLSELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIVDGKEAVGFLV
TIKNLLEDPEQLLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=51.219512195122, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.3448275862069, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=29.7592997811816, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=27.2517321016166, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=29.7592997811816, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=36.3095238095238, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1786946, Length=414, Percent_Identity=45.4106280193237, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=32.3185011709602, Blast_Score=181, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=414, Percent_Identity=39.1304347826087, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=30.365296803653, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=26.241134751773, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=33.1858407079646, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=41.6267942583732, Blast_Score=306, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=29.2410714285714, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24645909, Length=220, Percent_Identity=52.2727272727273, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=27.6995305164319, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI20129315, Length=442, Percent_Identity=27.3755656108597, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24582497, Length=430, Percent_Identity=27.6744186046512, Blast_Score=128, Evalue=8e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODO2_BACSU (P16263)

Other databases:

- EMBL:   M27141
- EMBL:   AL009126
- PIR:   B32879
- RefSeq:   NP_389818.2
- ProteinModelPortal:   P16263
- EnsemblBacteria:   EBBACT00000002447
- GeneID:   939505
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19360
- NMPDR:   fig|224308.1.peg.1941
- GenoList:   BSU19360
- GeneTree:   EBGT00050000001223
- HOGENOM:   HBG630916
- PhylomeDB:   P16263
- ProtClustDB:   PRK05704
- BioCyc:   BSUB:BSU19360-MONOMER
- BRENDA:   2.3.1.61
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10
- TIGRFAMs:   TIGR01347

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.61

Molecular weight: Translated: 46004; Mature: 45873

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 388-388 ACT_SITE 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLQEVLK
CCCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
DSGDTVQVGEIIGTISEGAGESSAPAPTEKTESKESVKEEKQAEPAAQEVSEEAQSEAKS
CCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RTIASPSARKLAREKGIDLSQVPTGDPLGRVRKQDVEAYEKPASKPAPQQKQQPQAQKAQ
HCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHH
QSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFFEQNE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKLGFMSFFTKAVVAALKKYPLLNAEIQGDELIVKKFYDIGIAVAADEGLVVPVVRDADR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCCC
LTFAGIEKEIGELAKKARNNKLTLSELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMH
HHHHHHHHHHHHHHHHHCCCCEEHEECCCCEEEEECCCCHHHHHHCCCCCCCCCCEEEEE
KIQLRPVAIDEERFENRPMMYIALSYDHRIVDGKEAVGFLVTIKNLLEDPEQLLLEG
EEEEEEEEECHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
AEIKVPELAESISEGTIAQWLKQPGDYVEQGEYLLELETDKVNVELTAEESGVLQEVLK
CCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
DSGDTVQVGEIIGTISEGAGESSAPAPTEKTESKESVKEEKQAEPAAQEVSEEAQSEAKS
CCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RTIASPSARKLAREKGIDLSQVPTGDPLGRVRKQDVEAYEKPASKPAPQQKQQPQAQKAQ
HCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHH
QSFDKPVEVQKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAVMNLRKRRKDQFFEQNE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKLGFMSFFTKAVVAALKKYPLLNAEIQGDELIVKKFYDIGIAVAADEGLVVPVVRDADR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCCC
LTFAGIEKEIGELAKKARNNKLTLSELQGGSFTITNGGTFGSLMSTPILNSPQVGILGMH
HHHHHHHHHHHHHHHHHCCCCEEHEECCCCEEEEECCCCHHHHHHCCCCCCCCCCEEEEE
KIQLRPVAIDEERFENRPMMYIALSYDHRIVDGKEAVGFLVTIKNLLEDPEQLLLEG
EEEEEEEEECHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377