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The map label for this gene is pksI

Identifier: 255767395

GI number: 255767395

Start: 1792012

End: 1792761

Strand: Direct

Name: pksI

Synonym: BSU17170

Alternate gene names: 255767395

Gene position: 1792012-1792761 (Clockwise)

Preceding gene: 16078777

Following gene: 255767396

Centisome position: 42.51

GC content: 43.87

Gene sequence:

>750_bases
ATGACGCATTCTGTTGTAGAGCTTATCGAAATTGAATCCGCCATTATTCAGGTAAAGATGCAAGATCGCACTCATAAAAA
CGCGTTTTCACAAGAACTTACAGACGATTTAATTCAAGCGTTTGAATACATACGGCAGAATCCCAAATACAAAGCGGTCA
TTTTGACAGGATATGATAATTATTTTGCTTCAGGAGGAACGCAAGAAGGACTGCTTCGAATTCAACAGGGATTGACCAAG
TTCACTGATGACAATCTTTATTCTCTGGCGCTGGATTGCGAGATTCCAGTTATTGCGGCAATGCAGGGCCATGGAATTGG
AGGCGGCTTTGTCATGGGGCTCTTTGCTGACATTGTCATTCTGAGCAGAGAAAGCGTGTATACGGCTAATTTTATGAAAT
ACGGATTTACGCCTGGAATGGGCGCTACCTTTATCGTTCCTAAAAAACTGGGTTTCAGCCTGGCCCAGGAAATATTATTG
AATGCGGGCAGCTACCGCGGAGCTGATCTTGAAAAAAGAGGGGTTCCTTTTAAGGTGCTGCCCCGTGCAGAAGTGTTAGA
TTATGCAGTGGAGCTGGCGCAAGAACTGGCGGAAAAGCCGAGAAACTCACTGGTTACACTGAAAGATCATTTGGTTGCCC
CGCTTCGCGATCAGCTGCCGCGCGTCATTGAACAGGAATTGATGATGCATGAGAAAACATTTCATCATGAAGAAGTCAAA
AGTAGAATCAAAGGTTTATATGGTAATTAA

Upstream 100 bases:

>100_bases
AAACCAAATGGGAATCATCAGGTATGTTGAAACAGGACAATTCCCATGGGAGGATCAGTAACCGTTTAAAAATGACAATC
AGAACCGTTAGGAGGACATC

Downstream 100 bases:

>100_bases
CTGAAAATATATATAAATTTTGCAGGTGAAATGGAGGCAGCGTGATGAGAAATAATGATAATATCCGCATATTAACTAAT
CCTTCTGTCAGTCATGGGGA

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTHSVVELIEIESAIIQVKMQDRTHKNAFSQELTDDLIQAFEYIRQNPKYKAVILTGYDNYFASGGTQEGLLRIQQGLTK
FTDDNLYSLALDCEIPVIAAMQGHGIGGGFVMGLFADIVILSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILL
NAGSYRGADLEKRGVPFKVLPRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRDQLPRVIEQELMMHEKTFHHEEVK
SRIKGLYGN

Sequences:

>Translated_249_residues
MTHSVVELIEIESAIIQVKMQDRTHKNAFSQELTDDLIQAFEYIRQNPKYKAVILTGYDNYFASGGTQEGLLRIQQGLTK
FTDDNLYSLALDCEIPVIAAMQGHGIGGGFVMGLFADIVILSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILL
NAGSYRGADLEKRGVPFKVLPRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRDQLPRVIEQELMMHEKTFHHEEVK
SRIKGLYGN
>Mature_248_residues
THSVVELIEIESAIIQVKMQDRTHKNAFSQELTDDLIQAFEYIRQNPKYKAVILTGYDNYFASGGTQEGLLRIQQGLTKF
TDDNLYSLALDCEIPVIAAMQGHGIGGGFVMGLFADIVILSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILLN
AGSYRGADLEKRGVPFKVLPRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRDQLPRVIEQELMMHEKTFHHEEVKS
RIKGLYGN

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to pksL

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=219, Percent_Identity=26.4840182648402, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI221142681, Length=233, Percent_Identity=23.6051502145923, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787659, Length=175, Percent_Identity=24.5714285714286, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788597, Length=200, Percent_Identity=27, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=214, Percent_Identity=27.1028037383178, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=200, Percent_Identity=25, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=27.3504273504274, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20129971, Length=214, Percent_Identity=25.2336448598131, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653477, Length=214, Percent_Identity=25.2336448598131, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PKSI_BACSU (P40802)

Other databases:

- EMBL:   U11039
- EMBL:   AL009126
- PIR:   G69678
- RefSeq:   NP_389597.2
- ProteinModelPortal:   P40802
- SMR:   P40802
- EnsemblBacteria:   EBBACT00000001533
- GeneID:   940096
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17170
- NMPDR:   fig|224308.1.peg.1719
- GenoList:   BSU17170
- GeneTree:   EBGT00070000032512
- HOGENOM:   HBG338004
- ProtClustDB:   PRK07110
- BioCyc:   BSUB:BSU17170-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27897; Mature: 27765

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSVVELIEIESAIIQVKMQDRTHKNAFSQELTDDLIQAFEYIRQNPKYKAVILTGYDN
CCHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH
YFASGGTQEGLLRIQQGLTKFTDDNLYSLALDCEIPVIAAMQGHGIGGGFVMGLFADIVI
HHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
LSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILLNAGSYRGADLEKRGVPFKVL
HCCCCHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEC
PRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRDQLPRVIEQELMMHEKTFHHEEVK
CHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRIKGLYGN
HHHHCCCCC
>Mature Secondary Structure 
THSVVELIEIESAIIQVKMQDRTHKNAFSQELTDDLIQAFEYIRQNPKYKAVILTGYDN
CHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH
YFASGGTQEGLLRIQQGLTKFTDDNLYSLALDCEIPVIAAMQGHGIGGGFVMGLFADIVI
HHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
LSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILLNAGSYRGADLEKRGVPFKVL
HCCCCHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEC
PRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRDQLPRVIEQELMMHEKTFHHEEVK
CHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRIKGLYGN
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377