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The map label for this gene is proA

Identifier: 255767309

GI number: 255767309

Start: 1379605

End: 1380852

Strand: Direct

Name: proA

Synonym: BSU13130

Alternate gene names: 255767309

Gene position: 1379605-1380852 (Clockwise)

Preceding gene: 255767308

Following gene: 16078379

Centisome position: 32.73

GC content: 47.36

Gene sequence:

>1248_bases
ATGAGTGAAGTTTCTGTAAAAGCGAAGCTGGCGAAAGAAGCAGCAGCCGAAATGATCATGAAAACAACAGCCGAGAAGGA
TGAGGCGCTCAGCCTCATTGCAAACGGACTCCGCAAAGAACTGGATTTTCTCTTGGCGGAGAATGCAAAAGACATTGTGA
ACGGAAAAGAGAATGGTTTAACACCGGACATCATTGACCGTCTCTCATTGGATGAGAAACGGATACGCGATATCGCGGAC
GCGGTGGAGCTCTTAATCGACTTAGCGGACCCAATCGGCGACTCTCTTGAAACGATTGAAAAAGAAAACGGCCTGTTTAT
TGAAAAAATCCGTGTGCCGCTCGGTGTCGTAGGAATGATTTATGAGGCGAGGCCAAACGTCACAGTTGATGCGGCTACCC
TTTGCCTGAAGACAGGGAACGCGGTTGTGCTGCGGGGAAGCTCCTCAGCCATCCACAGCAACAAAGCGCTCGTCAGTGTC
ATTTACAGAGCACTTGAGCAATCAGCGCTTCCGATTCACGCTGTGCAGCTGATTGAGGATACGAGCAGAGAGACAGCAAA
AGAGCTCTTTACGTTAAATGATGGCTTAGACGTATTGATTCCGCGCGGAGGCAAGAAACTGATTGATCTCGTTGTGAGAG
AATCAACAGTTCCTGTATTAGAAACGGGAGCAGGAAACTGCCACATATTCATTGACGAAACAGCCAAACCGCAAATGGCA
GAAAAGGTTGTTGTAAATGCCAAAACGCAGCGCCCTTCTGTATGCAACGCGATTGAATCATTGCTGATTCACAAGGCTTG
GGCAAGACAGCACGGAAAAGAATTGCTGGACCAGCTGGAAAACGCGGGAGTTGAAATTCGCGGTGATGAATTGGTATGTG
AACTTCATCCTTCAAGCAAACAAGCATCAAAAGAAGATTGGGAAACCGAATTTTTAGCGCCTGTCCTCAGCGTAAAGACG
GTTGAAAACGTCCAAGAAGCTGTAAAGCATATCCAACAATACGGCACCAATCATTCTGAAGCGATTTTAACTGAAAATGA
CAAAAATGCGGTATATTTTCAAACGGCTGTCGATGCTGCCGCTGTCTATCATAACGCGTCAACCCGCTTTACCGACGGCT
TTGAATTCGGCTACGGAGCCGAAATCGGCATCAGCACGCAAAAGCTTCATGCAAGAGGACCGATGGGGCTTCCTGCACTG
ACTTCTACAAAATACATCATTAAAGGAACTGGGCAAATCCGTGAATAG

Upstream 100 bases:

>100_bases
AGGCTAAAGGCAAACGCAGTGACGAACTTGATTTTGAGAAAACGTTTGAGGTTATTCATAGGAATGACTGGGTCAATGTA
AAAGACTAGGAGGCGGAAAA

Downstream 100 bases:

>100_bases
CGGGGTAATGTTCAATGAAAAAGCTTATCTCATTACGAGATAAGCTTTTTTATACGCGAAAAATTGACATTTCAAATTTA
ATTGTGTACAATTTAATTGT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSEVSVKAKLAKEAAAEMIMKTTAEKDEALSLIANGLRKELDFLLAENAKDIVNGKENGLTPDIIDRLSLDEKRIRDIAD
AVELLIDLADPIGDSLETIEKENGLFIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSV
IYRALEQSALPIHAVQLIEDTSRETAKELFTLNDGLDVLIPRGGKKLIDLVVRESTVPVLETGAGNCHIFIDETAKPQMA
EKVVVNAKTQRPSVCNAIESLLIHKAWARQHGKELLDQLENAGVEIRGDELVCELHPSSKQASKEDWETEFLAPVLSVKT
VENVQEAVKHIQQYGTNHSEAILTENDKNAVYFQTAVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLPAL
TSTKYIIKGTGQIRE

Sequences:

>Translated_415_residues
MSEVSVKAKLAKEAAAEMIMKTTAEKDEALSLIANGLRKELDFLLAENAKDIVNGKENGLTPDIIDRLSLDEKRIRDIAD
AVELLIDLADPIGDSLETIEKENGLFIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSV
IYRALEQSALPIHAVQLIEDTSRETAKELFTLNDGLDVLIPRGGKKLIDLVVRESTVPVLETGAGNCHIFIDETAKPQMA
EKVVVNAKTQRPSVCNAIESLLIHKAWARQHGKELLDQLENAGVEIRGDELVCELHPSSKQASKEDWETEFLAPVLSVKT
VENVQEAVKHIQQYGTNHSEAILTENDKNAVYFQTAVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLPAL
TSTKYIIKGTGQIRE
>Mature_414_residues
SEVSVKAKLAKEAAAEMIMKTTAEKDEALSLIANGLRKELDFLLAENAKDIVNGKENGLTPDIIDRLSLDEKRIRDIADA
VELLIDLADPIGDSLETIEKENGLFIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSVI
YRALEQSALPIHAVQLIEDTSRETAKELFTLNDGLDVLIPRGGKKLIDLVVRESTVPVLETGAGNCHIFIDETAKPQMAE
KVVVNAKTQRPSVCNAIESLLIHKAWARQHGKELLDQLENAGVEIRGDELVCELHPSSKQASKEDWETEFLAPVLSVKTV
ENVQEAVKHIQQYGTNHSEAILTENDKNAVYFQTAVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLPALT
STKYIIKGTGQIRE

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=418, Percent_Identity=33.0143540669856, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI62912457, Length=418, Percent_Identity=33.0143540669856, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=46.078431372549, Blast_Score=355, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=35.3510895883777, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=35.3510895883777, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324899, Length=437, Percent_Identity=32.4942791762014, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI21357643, Length=409, Percent_Identity=33.9853300733496, Blast_Score=245, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_BACSU (P39821)

Other databases:

- EMBL:   D26044
- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   C69682
- RefSeq:   NP_389196.2
- ProteinModelPortal:   P39821
- SMR:   P39821
- EnsemblBacteria:   EBBACT00000002003
- GeneID:   936166
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13130
- NMPDR:   fig|224308.1.peg.1315
- GenoList:   BSU13130
- GeneTree:   EBGT00050000000020
- HOGENOM:   HBG318080
- ProtClustDB:   PRK00197
- BioCyc:   BSUB:BSU13130-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45252; Mature: 45121

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVSVKAKLAKEAAAEMIMKTTAEKDEALSLIANGLRKELDFLLAENAKDIVNGKENGL
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
TPDIIDRLSLDEKRIRDIADAVELLIDLADPIGDSLETIEKENGLFIEKIRVPLGVVGMI
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEHHCCHHHHHHE
YEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSVIYRALEQSALPIHAVQLIED
EECCCCCEEHHHHHEEECCCEEEEECCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHH
TSRETAKELFTLNDGLDVLIPRGGKKLIDLVVRESTVPVLETGAGNCHIFIDETAKPQMA
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCHHH
EKVVVNAKTQRPSVCNAIESLLIHKAWARQHGKELLDQLENAGVEIRGDELVCELHPSSK
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCC
QASKEDWETEFLAPVLSVKTVENVQEAVKHIQQYGTNHSEAILTENDKNAVYFQTAVDAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHHHH
AVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLPALTSTKYIIKGTGQIRE
HHHCCCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
SEVSVKAKLAKEAAAEMIMKTTAEKDEALSLIANGLRKELDFLLAENAKDIVNGKENGL
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
TPDIIDRLSLDEKRIRDIADAVELLIDLADPIGDSLETIEKENGLFIEKIRVPLGVVGMI
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEHHCCHHHHHHE
YEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSVIYRALEQSALPIHAVQLIED
EECCCCCEEHHHHHEEECCCEEEEECCCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHH
TSRETAKELFTLNDGLDVLIPRGGKKLIDLVVRESTVPVLETGAGNCHIFIDETAKPQMA
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCHHH
EKVVVNAKTQRPSVCNAIESLLIHKAWARQHGKELLDQLENAGVEIRGDELVCELHPSSK
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCC
QASKEDWETEFLAPVLSVKTVENVQEAVKHIQQYGTNHSEAILTENDKNAVYFQTAVDAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEHHHHH
AVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLPALTSTKYIIKGTGQIRE
HHHCCCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8083159; 9384377