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The map label for this gene is proB

Identifier: 255767308

GI number: 255767308

Start: 1378496

End: 1379593

Strand: Direct

Name: proB

Synonym: BSU13120

Alternate gene names: 255767308

Gene position: 1378496-1379593 (Clockwise)

Preceding gene: 255767307

Following gene: 255767309

Centisome position: 32.7

GC content: 46.63

Gene sequence:

>1098_bases
ATGAAAAAACAAAGAATAGTAGTGAAAATAGGAAGCAGTTCGCTCACGAATAGCAAAGGAAGCATTGATGAGGCAAAAAT
CAGAGAGCATGTTCAGGCGATTTCCGTGTTAAAAAAAGCGGGGCATGAAATGATTCTGATTACCTCGGGTGCCGTAGCGG
CGGGGTTTTCCAGCCTCGGTTATCCATCCCGTCCCGTTACCATCAAAGGAAAACAGGCGGCGGCCGCGGTCGGACAAACA
CTGTTAATGCAGCAATATATGAATCAATTTAAACAATACTCACTGACTCCGGGACAAATCCTTTTAACGAGAAATGATTT
TTCGAAAAGAGAACGGTATCGAAACGCGTATGCTACGATAATGGAATTATTAGAGCGGGGCGTCATTCCGATTATCAACG
AGAACGATTCTACATCTGTTGAAGAATTGACATTCGGAGATAATGATATGCTTTCTGCGTTAGTCAGCGGATTGATTCAC
GCGGACCAGCTCATGATTCTCACTGACATTAACGGACTGTATGATGCCAATCCAAATGAAAATCCTGAGGCGAAACGATT
TGATTATTTGCCAGAGATCACGCCTGAATTGCTTGGATATGCAGGTTCAGCTGGATCAAAGGTCGGCACCGGCGGAATGA
AATCAAAGCTGTTAGCCACACAAACCGCGCTGTCTCTGGGAGTGAAAGTATTCATTGGAACTGGCAGCGGAGAGCAAAAA
CTTGCGGACATTTTGGACGGCAGGGGAGACGGCACTTATATCGGAGACAAAGAACTATCTTCGGTTAACAACACAAGGCA
GTGGATTCAGTTCCACTCGCCGATATCGGGAGAAATTATCATTGATGCGGGTGCCGAAGAGGCGATGATCCATAATGGAA
GCAGCCTTTTGCCCGCTGGAGTTGTGGGCGTGAACGGAAGCTTTCCGAAAGGGGCGGTTGTGGAAGTCAGGGGACCGGGC
GGCGTGATCGGCAAAGGCCAAACTCATTACTCCTCCGAGGAGATTATGGAGGCTAAAGGCAAACGCAGTGACGAACTTGA
TTTTGAGAAAACGTTTGAGGTTATTCATAGGAATGACTGGGTCAATGTAAAAGACTAG

Upstream 100 bases:

>100_bases
AGCGCGTTCAATCAAGGTGGTACCACGGAAACCCATTTCGTCCTTATGAATCAGGATGAAATGGGTTTTTTTATTGTAGA
AGAGCCGCGGAGGAGAATAA

Downstream 100 bases:

>100_bases
GAGGCGGAAAAATGAGTGAAGTTTCTGTAAAAGCGAAGCTGGCGAAAGAAGCAGCAGCCGAAATGATCATGAAAACAACA
GCCGAGAAGGATGAGGCGCT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 1; GK 1

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKKQRIVVKIGSSSLTNSKGSIDEAKIREHVQAISVLKKAGHEMILITSGAVAAGFSSLGYPSRPVTIKGKQAAAAVGQT
LLMQQYMNQFKQYSLTPGQILLTRNDFSKRERYRNAYATIMELLERGVIPIINENDSTSVEELTFGDNDMLSALVSGLIH
ADQLMILTDINGLYDANPNENPEAKRFDYLPEITPELLGYAGSAGSKVGTGGMKSKLLATQTALSLGVKVFIGTGSGEQK
LADILDGRGDGTYIGDKELSSVNNTRQWIQFHSPISGEIIIDAGAEEAMIHNGSSLLPAGVVGVNGSFPKGAVVEVRGPG
GVIGKGQTHYSSEEIMEAKGKRSDELDFEKTFEVIHRNDWVNVKD

Sequences:

>Translated_365_residues
MKKQRIVVKIGSSSLTNSKGSIDEAKIREHVQAISVLKKAGHEMILITSGAVAAGFSSLGYPSRPVTIKGKQAAAAVGQT
LLMQQYMNQFKQYSLTPGQILLTRNDFSKRERYRNAYATIMELLERGVIPIINENDSTSVEELTFGDNDMLSALVSGLIH
ADQLMILTDINGLYDANPNENPEAKRFDYLPEITPELLGYAGSAGSKVGTGGMKSKLLATQTALSLGVKVFIGTGSGEQK
LADILDGRGDGTYIGDKELSSVNNTRQWIQFHSPISGEIIIDAGAEEAMIHNGSSLLPAGVVGVNGSFPKGAVVEVRGPG
GVIGKGQTHYSSEEIMEAKGKRSDELDFEKTFEVIHRNDWVNVKD
>Mature_365_residues
MKKQRIVVKIGSSSLTNSKGSIDEAKIREHVQAISVLKKAGHEMILITSGAVAAGFSSLGYPSRPVTIKGKQAAAAVGQT
LLMQQYMNQFKQYSLTPGQILLTRNDFSKRERYRNAYATIMELLERGVIPIINENDSTSVEELTFGDNDMLSALVSGLIH
ADQLMILTDINGLYDANPNENPEAKRFDYLPEITPELLGYAGSAGSKVGTGGMKSKLLATQTALSLGVKVFIGTGSGEQK
LADILDGRGDGTYIGDKELSSVNNTRQWIQFHSPISGEIIIDAGAEEAMIHNGSSLLPAGVVGVNGSFPKGAVVEVRGPG
GVIGKGQTHYSSEEIMEAKGKRSDELDFEKTFEVIHRNDWVNVKD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=30.9027777777778, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=39.8351648351648, Blast_Score=259, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=34.6863468634686, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=34.6863468634686, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=35.0961538461538, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=33.5731414868106, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21357643, Length=283, Percent_Identity=31.8021201413428, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_BACSU (P39820)

Other databases:

- EMBL:   D26044
- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   D69682
- RefSeq:   NP_389195.2
- ProteinModelPortal:   P39820
- SMR:   P39820
- EnsemblBacteria:   EBBACT00000001169
- GeneID:   936790
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13120
- NMPDR:   fig|224308.1.peg.1314
- GenoList:   BSU13120
- GeneTree:   EBGT00050000001887
- HOGENOM:   HBG507643
- ProtClustDB:   PRK05429
- BioCyc:   BSUB:BSU13120-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39346; Mature: 39346

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRIVVKIGSSSLTNSKGSIDEAKIREHVQAISVLKKAGHEMILITSGAVAAGFSSLG
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCC
YPSRPVTIKGKQAAAAVGQTLLMQQYMNQFKQYSLTPGQILLTRNDFSKRERYRNAYATI
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
MELLERGVIPIINENDSTSVEELTFGDNDMLSALVSGLIHADQLMILTDINGLYDANPNE
HHHHHCCCCEEECCCCCCCHHEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
NPEAKRFDYLPEITPELLGYAGSAGSKVGTGGMKSKLLATQTALSLGVKVFIGTGSGEQK
CCCCHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
LADILDGRGDGTYIGDKELSSVNNTRQWIQFHSPISGEIIIDAGAEEAMIHNGSSLLPAG
HHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHEEECCCCCCCCC
VVGVNGSFPKGAVVEVRGPGGVIGKGQTHYSSEEIMEAKGKRSDELDFEKTFEVIHRNDW
EEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
VNVKD
EECCC
>Mature Secondary Structure
MKKQRIVVKIGSSSLTNSKGSIDEAKIREHVQAISVLKKAGHEMILITSGAVAAGFSSLG
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCC
YPSRPVTIKGKQAAAAVGQTLLMQQYMNQFKQYSLTPGQILLTRNDFSKRERYRNAYATI
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
MELLERGVIPIINENDSTSVEELTFGDNDMLSALVSGLIHADQLMILTDINGLYDANPNE
HHHHHCCCCEEECCCCCCCHHEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
NPEAKRFDYLPEITPELLGYAGSAGSKVGTGGMKSKLLATQTALSLGVKVFIGTGSGEQK
CCCCHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
LADILDGRGDGTYIGDKELSSVNNTRQWIQFHSPISGEIIIDAGAEEAMIHNGSSLLPAG
HHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHEEECCCCCCCCC
VVGVNGSFPKGAVVEVRGPGGVIGKGQTHYSSEEIMEAKGKRSDELDFEKTFEVIHRNDW
EEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
VNVKD
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083159; 9384377