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The map label for this gene is ykfA

Identifier: 255767303

GI number: 255767303

Start: 1365888

End: 1366847

Strand: Direct

Name: ykfA

Synonym: BSU12970

Alternate gene names: 255767303

Gene position: 1365888-1366847 (Clockwise)

Preceding gene: 255767302

Following gene: 16078363

Centisome position: 32.4

GC content: 50.0

Gene sequence:

>960_bases
ATGAAAGGAGTGTTTTCGTTGAATTACAAGCCGAAAGCGTTGAACAAGGGTGATACAGTCGGAGTGATCGCGCCCGCAAG
TCCGCCGGATCCAAAAAAGCTTGACACCGCGCTTTTATTTTTAGAAGAGCTCGGTCTTCAGGTGAAGTTGGGCAAGGCGC
TGAAAAACCAGCACGGCTATTTAGCGGGACAGGATGATGAGCGGCTGGCGGATCTCCATGAGATGTTCAGAGACGATGAG
GTAAAAGCAGTGTTGTGCGCATGCGGGGGTTTTGGGACAGGACGTATCGCCGCGGGCATTGATTTCAGCTTGATCCGCAA
ACACCCTAAAATCTTTTGGGGATACAGCGATATTACGTTTTTACATACTGCCATTCATCAAAACACAGGTCTTGTCACTT
TCCATGGCCCGATGCTCAGCACGGATATTGGCCTTGACGACGTTCACCCGCTGACAAAAGCGTCATATAAGCAGCTCTTC
CAGGAGACGGAATTCACCTATACAGAAGAGCTTTCTCCGCTGACCGAGCTTGTTCCTGGAAAAGCGGAAGGCGAGCTTGT
CGGGGGAAATCTGTCTTTGCTGACGTCTACACTGGGCACGCCATTTGAAATTGATACGAGAGGAAAGCTTCTGTTTATTG
AAGATATTGACGAGGAGCCTTATCAAATCGACCGGATGCTGAATCAGCTGAAAATGGGGGGGAAGCTGACGGACGCGGCG
GGAATTCTAGTTTGTGATTTTCACAATTGTGTCCCGGTGAAGCGAGAGAAGTCTCTCTCGCTTGAGCAGGTGCTGGAAGA
CTATATTATTTCTGCGGGCAGGCCTGCTCTGAGAGGATTTAAAATCGGCCACTGCTCGCCAAGTATTGCCGTTCCGATCG
GTGCGAAAGCTGCTATGAATACAGCAGAAAAAACAGCCGTAATAGAGGCGGGCGTTTCAGAAGGGGCGCTGAAGACATGA

Upstream 100 bases:

>100_bases
AGATAAAAACTGATGGAGGCGATTGAGGAAATACTGCTTCTTTATGCGAAGGAGCGGTATTTTTTCTCTTTCTTGCACGT
ATACGTAGGGTGCAGAGCAA

Downstream 100 bases:

>100_bases
AAATCATTCGAATCGAAACAAGCCGAATCGCTGTCCCGCTGACAAAGCCGTTTAAAACCGCACTTCGCACTGTGTATACG
GCTGAATCAGTCATAGTAAG

Product: L,D-carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKGVFSLNYKPKALNKGDTVGVIAPASPPDPKKLDTALLFLEELGLQVKLGKALKNQHGYLAGQDDERLADLHEMFRDDE
VKAVLCACGGFGTGRIAAGIDFSLIRKHPKIFWGYSDITFLHTAIHQNTGLVTFHGPMLSTDIGLDDVHPLTKASYKQLF
QETEFTYTEELSPLTELVPGKAEGELVGGNLSLLTSTLGTPFEIDTRGKLLFIEDIDEEPYQIDRMLNQLKMGGKLTDAA
GILVCDFHNCVPVKREKSLSLEQVLEDYIISAGRPALRGFKIGHCSPSIAVPIGAKAAMNTAEKTAVIEAGVSEGALKT

Sequences:

>Translated_319_residues
MKGVFSLNYKPKALNKGDTVGVIAPASPPDPKKLDTALLFLEELGLQVKLGKALKNQHGYLAGQDDERLADLHEMFRDDE
VKAVLCACGGFGTGRIAAGIDFSLIRKHPKIFWGYSDITFLHTAIHQNTGLVTFHGPMLSTDIGLDDVHPLTKASYKQLF
QETEFTYTEELSPLTELVPGKAEGELVGGNLSLLTSTLGTPFEIDTRGKLLFIEDIDEEPYQIDRMLNQLKMGGKLTDAA
GILVCDFHNCVPVKREKSLSLEQVLEDYIISAGRPALRGFKIGHCSPSIAVPIGAKAAMNTAEKTAVIEAGVSEGALKT
>Mature_319_residues
MKGVFSLNYKPKALNKGDTVGVIAPASPPDPKKLDTALLFLEELGLQVKLGKALKNQHGYLAGQDDERLADLHEMFRDDE
VKAVLCACGGFGTGRIAAGIDFSLIRKHPKIFWGYSDITFLHTAIHQNTGLVTFHGPMLSTDIGLDDVHPLTKASYKQLF
QETEFTYTEELSPLTELVPGKAEGELVGGNLSLLTSTLGTPFEIDTRGKLLFIEDIDEEPYQIDRMLNQLKMGGKLTDAA
GILVCDFHNCVPVKREKSLSLEQVLEDYIISAGRPALRGFKIGHCSPSIAVPIGAKAAMNTAEKTAVIEAGVSEGALKT

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family

Homologues:

Organism=Escherichia coli, GI1787441, Length=277, Percent_Identity=29.9638989169675, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LDC_BACSU (O34851)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   G69855
- RefSeq:   NP_389180.2
- ProteinModelPortal:   O34851
- SMR:   O34851
- MEROPS:   S66.001
- EnsemblBacteria:   EBBACT00000004066
- GeneID:   938175
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12970
- NMPDR:   fig|224308.1.peg.1299
- GenoList:   BSU12970
- GeneTree:   EBGT00050000001771
- HOGENOM:   HBG497193
- ProtClustDB:   CLSK904776
- BioCyc:   BSUB:BSU12970-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003507

Pfam domain/function: PF02016 Peptidase_S66

EC number: NA

Molecular weight: Translated: 34596; Mature: 34596

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: ACT_SITE 116-116 ACT_SITE 214-214 ACT_SITE 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVFSLNYKPKALNKGDTVGVIAPASPPDPKKLDTALLFLEELGLQVKLGKALKNQHGY
CCCEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECHHHHCCCCC
LAGQDDERLADLHEMFRDDEVKAVLCACGGFGTGRIAAGIDFSLIRKHPKIFWGYSDITF
CCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCHHHHHCCCCEEECCCHHHH
LHTAIHQNTGLVTFHGPMLSTDIGLDDVHPLTKASYKQLFQETEFTYTEELSPLTELVPG
HHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCC
KAEGELVGGNLSLLTSTLGTPFEIDTRGKLLFIEDIDEEPYQIDRMLNQLKMGGKLTDAA
CCCCEEECCCHHHHHHHCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEECCC
GILVCDFHNCVPVKREKSLSLEQVLEDYIISAGRPALRGFKIGHCSPSIAVPIGAKAAMN
CEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCEEEECCCCCEEEECCCHHHHC
TAEKTAVIEAGVSEGALKT
HHHHHHEEECCCCCCCCCC
>Mature Secondary Structure
MKGVFSLNYKPKALNKGDTVGVIAPASPPDPKKLDTALLFLEELGLQVKLGKALKNQHGY
CCCEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECHHHHCCCCC
LAGQDDERLADLHEMFRDDEVKAVLCACGGFGTGRIAAGIDFSLIRKHPKIFWGYSDITF
CCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCHHHHHCCCCEEECCCHHHH
LHTAIHQNTGLVTFHGPMLSTDIGLDDVHPLTKASYKQLFQETEFTYTEELSPLTELVPG
HHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCC
KAEGELVGGNLSLLTSTLGTPFEIDTRGKLLFIEDIDEEPYQIDRMLNQLKMGGKLTDAA
CCCCEEECCCHHHHHHHCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEECCC
GILVCDFHNCVPVKREKSLSLEQVLEDYIISAGRPALRGFKIGHCSPSIAVPIGAKAAMN
CEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCEEEECCCCCEEEECCCHHHHC
TAEKTAVIEAGVSEGALKT
HHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377