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The map label for this gene is htrA

Identifier: 255767300

GI number: 255767300

Start: 1357936

End: 1359285

Strand: Reverse

Name: htrA

Synonym: BSU12900

Alternate gene names: 255767300

Gene position: 1359285-1357936 (Counterclockwise)

Preceding gene: 16078366

Following gene: 16078351

Centisome position: 32.24

GC content: 44.59

Gene sequence:

>1350_bases
ATGGATAACTATCGTGATGAAAACAGAACGAAAGGTAATGAGAATGAGGTCTTTTTAACGAAAGAGAACGATCAGAGCGC
CTCCTACTCGGCCCGCAATGTCATTCATGATCAGGAGAAGAAAAAACGAGGATTCGGATGGTTCAGACCGTTGCTTGGCG
GAGTGATCGGCGGCAGTCTTGCTCTTGGCATTTACACGTTTACACCGCTTGGTGACCATGATTCTCAGGACACTGCAAAA
CAATCATCCAGCCAGCAGCAAACGCAATCTGTTACAGCAACAAGCACCTCCTCTGAATCTAAAAAAAGCTCAAGCAGCTC
ATCTGCATTCAAGAGCGAGGACTCTTCTAAAATCTCAGATATGGTAGAAGACCTTTCACCAGCGATTGTCGGTATTACAA
ATCTTCAGGCACAATCAAACAGCTCTTTGTTCGGCTCTAGTTCTTCTGATTCCAGCGAAGATACAGAAAGCGGTTCAGGG
TCAGGTGTCATTTTCAAAAAAGAGAATGGCAAGGCTTATATCATTACAAATAACCACGTCGTAGAAGGGGCATCATCACT
GAAGGTATCTTTATATGACGGCACTGAGGTTACTGCAAAGCTGGTAGGCAGTGACTCGTTAACTGATTTAGCCGTCCTCC
AAATCAGTGATGACCACGTCACAAAAGTGGCAAACTTCGGTGATTCATCTGATCTTAGAACAGGCGAGACCGTTATTGCG
ATTGGGGATCCGCTTGGAAAAGACCTGTCCCGCACAGTAACACAAGGAATTGTAAGCGGCGTGGACAGAACGGTTTCAAT
GTCTACATCAGCCGGCGAAACGAGCATTAACGTCATTCAGACAGACGCAGCAATTAATCCAGGTAACAGCGGCGGTCCTT
TGTTAAATACAGACGGCAAAATTGTCGGCATTAACAGTATGAAAATCAGTGAGGATGATGTTGAGGGTATCGGATTCGCC
ATTCCAAGCAATGACGTAAAACCGATTGCTGAAGAATTGCTGTCTAAAGGACAAATTGAACGTCCATATATCGGTGTCAG
CATGCTTGATCTAGAGCAAGTGCCGCAAAATTACCAAGAAGGCACACTCGGCCTGTTCGGCAGCCAGCTGAATAAAGGCG
TTTACATCCGTGAGGTCGCTTCAGGCTCTCCTGCTGAAAAGGCCGGATTAAAAGCGGAGGATATTATCATCGGCCTAAAA
GGTAAAGAAATTGATACAGGCAGTGAATTGCGCAATATCTTATATAAAGACGCAAAGATCGGTGATACCGTTGAAGTGAA
AATTCTCCGAAACGGCAAAGAAATGACGAAAAAAATTAAACTGGATCAAAAAGAAGAGAAAACTTCGTAA

Upstream 100 bases:

>100_bases
CTTTTCATTTTTATCCCACAGTTTTTGTTTATGATAAACTCAAGTCATAAACCTATCAATATAAATAGACATGTGAAAAT
AGAGAAACGGAGTGAACATG

Downstream 100 bases:

>100_bases
GACATAATGCCTCAGGCCGTAAAAGCGGTCTGAGGCTTTTTATTAGATAAAAGACCTAATTGTTCTATGAAACTTTCTGA
CGAGATATCCGTATATCATA

Product: membrane bound serine protease Do

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSLALGIYTFTPLGDHDSQDTAK
QSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISDMVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSG
SGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFA
IPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLK
GKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEKTS

Sequences:

>Translated_449_residues
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSLALGIYTFTPLGDHDSQDTAK
QSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISDMVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSG
SGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFA
IPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLK
GKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEKTS
>Mature_449_residues
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSLALGIYTFTPLGDHDSQDTAK
QSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISDMVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSG
SGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFA
IPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLK
GKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEKTS

Specific function: May be involved in processing, maturation, or secretion of extracellular enzymes

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain

Homologues:

Organism=Homo sapiens, GI4506141, Length=322, Percent_Identity=34.7826086956522, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI22129776, Length=312, Percent_Identity=33.974358974359, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI7019477, Length=329, Percent_Identity=33.1306990881459, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI24308541, Length=311, Percent_Identity=32.7974276527331, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1789629, Length=335, Percent_Identity=37.0149253731343, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1786356, Length=391, Percent_Identity=32.9923273657289, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789630, Length=360, Percent_Identity=31.9444444444444, Blast_Score=148, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24646839, Length=344, Percent_Identity=34.593023255814, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HTRA_BACSU (O34358)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   A69643
- RefSeq:   NP_389173.2
- ProteinModelPortal:   O34358
- SMR:   O34358
- EnsemblBacteria:   EBBACT00000002851
- GeneID:   939849
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12900
- NMPDR:   fig|224308.1.peg.1292
- GenoList:   BSU12900
- GeneTree:   EBGT00050000001145
- HOGENOM:   HBG585708
- ProtClustDB:   CLSK887151
- BioCyc:   BSUB:BSU12900-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR001254
- InterPro:   IPR001940
- PRINTS:   PR00834
- SMART:   SM00228

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin; SSF50156 PDZ; SSF50494 Pept_Ser_Cys

EC number: 3.4.21.- [C]

Molecular weight: Translated: 47715; Mature: 47715

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50106 PDZ

Important sites: ACT_SITE 179-179 ACT_SITE 209-209 ACT_SITE 290-290

Signals:

None

Transmembrane regions:

HASH(0xe1ab54c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSL
CCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALGIYTFTPLGDHDSQDTAKQSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISD
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCCHHHCCCCHHHHHH
MVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSGSGVIFKKENGKAYIITNNHV
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCEE
VEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
ECCCCCEEEEEECCCCEEEEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCEEEE
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGK
ECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCEECCCCCCCCEECCCCC
IVGINSMKISEDDVEGIGFAIPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQE
EEEEECEEECCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCC
GTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLKGKEIDTGSELRNILYKDAKI
CCHHHHHHHHCCCEEEEEECCCCCHHHHCCCCHHEEEEECCCCCCCHHHHHHHHHHCCCC
GDTVEVKILRNGKEMTKKIKLDQKEEKTS
CCEEEEEEECCCHHHHHHHCCCCHHCCCH
>Mature Secondary Structure
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSL
CCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALGIYTFTPLGDHDSQDTAKQSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISD
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCCHHHCCCCHHHHHH
MVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSGSGVIFKKENGKAYIITNNHV
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCEE
VEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
ECCCCCEEEEEECCCCEEEEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCEEEE
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGK
ECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCEECCCCCCCCEECCCCC
IVGINSMKISEDDVEGIGFAIPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQE
EEEEECEEECCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCC
GTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLKGKEIDTGSELRNILYKDAKI
CCHHHHHHHHCCCEEEEEECCCCCHHHHCCCCHHEEEEECCCCCCCHHHHHHHHHHCCCC
GDTVEVKILRNGKEMTKKIKLDQKEEKTS
CCEEEEEEECCCHHHHHHHCCCCHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10692364; 11133960; 11555295