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The map label for this gene is manP

Identifier: 255767271

GI number: 255767271

Start: 1272725

End: 1274677

Strand: Direct

Name: manP

Synonym: BSU12010

Alternate gene names: 255767271

Gene position: 1272725-1274677 (Clockwise)

Preceding gene: 255767270

Following gene: 16078267

Centisome position: 30.19

GC content: 46.03

Gene sequence:

>1953_bases
ATGAAACTGTTAGCGATTACATCTTGTCCGAATGGAATCGCCCATACGTATATGGCAGCAGAAAATCTGCAAAAAGCTGC
TGACAGACTGGGCGTTAGCATCAAAGTTGAGACCCAGGGAGGGATTGGTGTTGAAAATAAGCTAACCGAAGAGGAAATTC
GCGAAGCGGACGCAATCATTATTGCTGCAGACCGTTCGGTAAACAAAGACCGGTTTATAGGCAAAAAACTGCTGTCTGTG
GGGGTACAGGACGGTATCCGCAAACCGGAAGAATTGATTCAAAAAGCTCTTAACGGGGATATACCGGTCTATCGTTCCGC
TACAAAATCGGAATCCGGTAATCATCAGGAGAAGAAACAAAATCCAATTTACCGTCATTTAATGAACGGCGTTTCATTTA
TGGTTCCTTTCATTGTTGTCGGCGGATTATTGATAGCGGTTGCATTAACGCTTGGAGGGGAGAAAACACCTAAAGGCTTA
GTTATTCCGGATGATTCCTTCTGGAAAACGATTGAACAGATCGGCAGTGCTTCATTTTCATTTATGATTCCGATTTTAGC
CGGCTATATCGCCTATAGCATCGCTGATAAACCGGGGCTTGTGCCAGGTATGATCGGCGGTTATATTGCGGCGACTGGAA
GTTTTTATGACAGCGCAAGCGGCGCCGGATTCCTCGGGGGGATTATTGCCGGGTTTTTGGCCGGATACGCGGCACTTTGG
ATCAAAAAATTAAAAGTTCCAAAAGCCATCCAGCCGATTATGCCGATTATTATTATTCCGGTGTTTGCATCACTGATTGT
AGGTTTAGCGTTTGTGTTTTTGATCGGCGCGCCTGTAGCTCAAATTTTCGCCTCTTTAACAGTTTGGCTGGCGGGGATGA
AAGGGTCGAGCTCGATCCTTCTGGCATTGATTTTAGGGGCGATGATCTCATTTGATATGGGCGGTCCCGTAAATAAGGTG
GCGTTTCTGTTTGGATCGGCAATGATTGGAGAAGGAAATTATGAAATTATGGGACCAATTGCTGTCGCCATTTGTATCCC
CCCGATTGGCCTTGGCATTGCGACGTTTTTAGGAAAAAGAAAATTCGAGGCATCTCAAAGAGAAATGGGGAAAGCGGCAT
TTACGATGGGGCTCTTCGGCATTACTGAAGGAGCGATTCCATTTGCGGCGCAGGACCCTCTGCGCGTTATTCCAAGTATT
ATGGCAGGCTCAATGACAGGTTCTGTGATAGCCATGATTGGCAATGTGGGAGACAGAGTGGCGCACGGCGGACCTATCGT
TGCAGTGCTTGGCGCAGTTGATCATGTCTTGATGTTTTTTATTGCTGTGATTGCAGGATCTCTTGTCACTGCTCTGTTCG
TCAATGTACTAAAAAAGGATATTACGGCGTCTCCTGTGCTCAGTGAAACTGCACCGACCTCCGCGCCAAGTGAAGCTGCG
GCAGCAAATGAAATAAAGCAGCCAATTCAAAGCCAAAAAGCTGAGATGTCGGAATTTAAAAAACTGACAGACATTATCAG
CCCGGAATTAATTGAACCAAATTTATCGGGGGAAACGAGTGATGACATCATAGATGAATTGATTCAGAAATTGTCACGAA
GAGGTGCGCTGCTTTCAGAGAGCGGGTTTAAACAAGCCATTTTGAATCGTGAACAACAGGGAACAACGGCGATTGGCATG
AATATCGCGATTCCGCACGGAAAGTCTGAGGCGGTCAGGGAGCCGAGTGTTGCCTTTGGGATCAAACGATCAGGCGTTGA
TTGGAATAGCCTGGATGGATCAGAAGCTAAATTAATCTTTATGATTGCTGTACCGAAAGAGAGCGGGGGAAACCAGCATC
TCAAAATACTGCAGATGCTATCTCGAAAGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAA
GAAGCATACAAGCTGCTAGAAGAAATCGAATAA

Upstream 100 bases:

>100_bases
TATTACCGGAACCTATGGTAAAAAAAGCGATTTTAATGAGCTGATTTCGGTATACAGTTGAGACAAGATCTTATTATTCA
CACTTTAAAGGAGGTTTCCT

Downstream 100 bases:

>100_bases
AGCGGGGGATTTTCATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTA
GCTGATTTTGGCTATACCAT

Product: phosphotransferase system (PTS) mannose-specific enzyme IIBCA component

Products: NA

Alternate protein names: EIIBCA-Man; EII-Man; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component; Mannose permease IIC component; PTS system mannose-specific EIIC component; Mannose-specific phosphotransferase enzyme IIA component; PTS system mannose-specific EIIA component

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MKLLAITSCPNGIAHTYMAAENLQKAADRLGVSIKVETQGGIGVENKLTEEEIREADAIIIAADRSVNKDRFIGKKLLSV
GVQDGIRKPEELIQKALNGDIPVYRSATKSESGNHQEKKQNPIYRHLMNGVSFMVPFIVVGGLLIAVALTLGGEKTPKGL
VIPDDSFWKTIEQIGSASFSFMIPILAGYIAYSIADKPGLVPGMIGGYIAATGSFYDSASGAGFLGGIIAGFLAGYAALW
IKKLKVPKAIQPIMPIIIIPVFASLIVGLAFVFLIGAPVAQIFASLTVWLAGMKGSSSILLALILGAMISFDMGGPVNKV
AFLFGSAMIGEGNYEIMGPIAVAICIPPIGLGIATFLGKRKFEASQREMGKAAFTMGLFGITEGAIPFAAQDPLRVIPSI
MAGSMTGSVIAMIGNVGDRVAHGGPIVAVLGAVDHVLMFFIAVIAGSLVTALFVNVLKKDITASPVLSETAPTSAPSEAA
AANEIKQPIQSQKAEMSEFKKLTDIISPELIEPNLSGETSDDIIDELIQKLSRRGALLSESGFKQAILNREQQGTTAIGM
NIAIPHGKSEAVREPSVAFGIKRSGVDWNSLDGSEAKLIFMIAVPKESGGNQHLKILQMLSRKLMDDNYRERLLSVQTTE
EAYKLLEEIE

Sequences:

>Translated_650_residues
MKLLAITSCPNGIAHTYMAAENLQKAADRLGVSIKVETQGGIGVENKLTEEEIREADAIIIAADRSVNKDRFIGKKLLSV
GVQDGIRKPEELIQKALNGDIPVYRSATKSESGNHQEKKQNPIYRHLMNGVSFMVPFIVVGGLLIAVALTLGGEKTPKGL
VIPDDSFWKTIEQIGSASFSFMIPILAGYIAYSIADKPGLVPGMIGGYIAATGSFYDSASGAGFLGGIIAGFLAGYAALW
IKKLKVPKAIQPIMPIIIIPVFASLIVGLAFVFLIGAPVAQIFASLTVWLAGMKGSSSILLALILGAMISFDMGGPVNKV
AFLFGSAMIGEGNYEIMGPIAVAICIPPIGLGIATFLGKRKFEASQREMGKAAFTMGLFGITEGAIPFAAQDPLRVIPSI
MAGSMTGSVIAMIGNVGDRVAHGGPIVAVLGAVDHVLMFFIAVIAGSLVTALFVNVLKKDITASPVLSETAPTSAPSEAA
AANEIKQPIQSQKAEMSEFKKLTDIISPELIEPNLSGETSDDIIDELIQKLSRRGALLSESGFKQAILNREQQGTTAIGM
NIAIPHGKSEAVREPSVAFGIKRSGVDWNSLDGSEAKLIFMIAVPKESGGNQHLKILQMLSRKLMDDNYRERLLSVQTTE
EAYKLLEEIE
>Mature_650_residues
MKLLAITSCPNGIAHTYMAAENLQKAADRLGVSIKVETQGGIGVENKLTEEEIREADAIIIAADRSVNKDRFIGKKLLSV
GVQDGIRKPEELIQKALNGDIPVYRSATKSESGNHQEKKQNPIYRHLMNGVSFMVPFIVVGGLLIAVALTLGGEKTPKGL
VIPDDSFWKTIEQIGSASFSFMIPILAGYIAYSIADKPGLVPGMIGGYIAATGSFYDSASGAGFLGGIIAGFLAGYAALW
IKKLKVPKAIQPIMPIIIIPVFASLIVGLAFVFLIGAPVAQIFASLTVWLAGMKGSSSILLALILGAMISFDMGGPVNKV
AFLFGSAMIGEGNYEIMGPIAVAICIPPIGLGIATFLGKRKFEASQREMGKAAFTMGLFGITEGAIPFAAQDPLRVIPSI
MAGSMTGSVIAMIGNVGDRVAHGGPIVAVLGAVDHVLMFFIAVIAGSLVTALFVNVLKKDITASPVLSETAPTSAPSEAA
AANEIKQPIQSQKAEMSEFKKLTDIISPELIEPNLSGETSDDIIDELIQKLSRRGALLSESGFKQAILNREQQGTTAIGM
NIAIPHGKSEAVREPSVAFGIKRSGVDWNSLDGSEAKLIFMIAVPKESGGNQHLKILQMLSRKLMDDNYRERLLSVQTTE
EAYKLLEEIE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI1788492, Length=465, Percent_Identity=44.0860215053763, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1786951, Length=425, Percent_Identity=40.2352941176471, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI87082348, Length=471, Percent_Identity=35.244161358811, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1790386, Length=306, Percent_Identity=45.7516339869281, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1788729, Length=380, Percent_Identity=29.7368421052632, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1790387, Length=95, Percent_Identity=44.2105263157895, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788730, Length=87, Percent_Identity=41.3793103448276, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI2367327, Length=128, Percent_Identity=28.90625, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790390, Length=94, Percent_Identity=39.3617021276596, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789597, Length=126, Percent_Identity=30.952380952381, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788726, Length=105, Percent_Identity=32.3809523809524, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI48994992, Length=144, Percent_Identity=22.9166666666667, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTN3B_BACSU (O31645)

Other databases:

- EMBL:   AL009126
- PIR:   G69848
- RefSeq:   NP_389083.2
- PDB:   2R48
- PDBsum:   2R48
- ProteinModelPortal:   O31645
- PhosSite:   O31645
- EnsemblBacteria:   EBBACT00000001983
- GeneID:   936437
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12010
- NMPDR:   fig|224308.1.peg.1202
- GenoList:   BSU12010
- GeneTree:   EBGT00050000000031
- HOGENOM:   HBG704192
- ProtClustDB:   CLSK886127
- BioCyc:   BSUB:BSU12010-MONOMER
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327
- Gene3D:   G3DSA:3.40.930.10
- TIGRFAMs:   TIGR00829
- TIGRFAMs:   TIGR00848
- TIGRFAMs:   TIGR01427

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB; SSF55804 PTrfase/Anion_transptr

EC number: =2.7.1.69

Molecular weight: Translated: 69027; Mature: 69027

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS; PS51099 PTS_EIIB_TYPE_2; PS51104 PTS_EIIC_TYPE_2

Important sites: ACT_SITE 9-9 ACT_SITE 566-566

Signals:

None

Transmembrane regions:

HASH(0xdf22ab0)-; HASH(0xe426660)-; HASH(0xe0dcb58)-; HASH(0xce96f98)-; HASH(0xbd2fed0)-; HASH(0xe426504)-; HASH(0xe2a5a7c)-; HASH(0xe2c01c4)-; HASH(0xc4a3da8)-; HASH(0xe2a0a8c)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLAITSCPNGIAHTYMAAENLQKAADRLGVSIKVETQGGIGVENKLTEEEIREADAII
CCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCEEE
IAADRSVNKDRFIGKKLLSVGVQDGIRKPEELIQKALNGDIPVYRSATKSESGNHQEKKQ
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHC
NPIYRHLMNGVSFMVPFIVVGGLLIAVALTLGGEKTPKGLVIPDDSFWKTIEQIGSASFS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHCCCCHH
FMIPILAGYIAYSIADKPGLVPGMIGGYIAATGSFYDSASGAGFLGGIIAGFLAGYAALW
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IKKLKVPKAIQPIMPIIIIPVFASLIVGLAFVFLIGAPVAQIFASLTVWLAGMKGSSSIL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHH
LALILGAMISFDMGGPVNKVAFLFGSAMIGEGNYEIMGPIAVAICIPPIGLGIATFLGKR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHH
KFEASQREMGKAAFTMGLFGITEGAIPFAAQDPLRVIPSIMAGSMTGSVIAMIGNVGDRV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AHGGPIVAVLGAVDHVLMFFIAVIAGSLVTALFVNVLKKDITASPVLSETAPTSAPSEAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AANEIKQPIQSQKAEMSEFKKLTDIISPELIEPNLSGETSDDIIDELIQKLSRRGALLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
SGFKQAILNREQQGTTAIGMNIAIPHGKSEAVREPSVAFGIKRSGVDWNSLDGSEAKLIF
CCHHHHHHCCCCCCCEEEEEEEECCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCCCEEEE
MIAVPKESGGNQHLKILQMLSRKLMDDNYRERLLSVQTTEEAYKLLEEIE
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MKLLAITSCPNGIAHTYMAAENLQKAADRLGVSIKVETQGGIGVENKLTEEEIREADAII
CCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCEEE
IAADRSVNKDRFIGKKLLSVGVQDGIRKPEELIQKALNGDIPVYRSATKSESGNHQEKKQ
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHC
NPIYRHLMNGVSFMVPFIVVGGLLIAVALTLGGEKTPKGLVIPDDSFWKTIEQIGSASFS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHCCCCHH
FMIPILAGYIAYSIADKPGLVPGMIGGYIAATGSFYDSASGAGFLGGIIAGFLAGYAALW
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IKKLKVPKAIQPIMPIIIIPVFASLIVGLAFVFLIGAPVAQIFASLTVWLAGMKGSSSIL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHH
LALILGAMISFDMGGPVNKVAFLFGSAMIGEGNYEIMGPIAVAICIPPIGLGIATFLGKR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHH
KFEASQREMGKAAFTMGLFGITEGAIPFAAQDPLRVIPSIMAGSMTGSVIAMIGNVGDRV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AHGGPIVAVLGAVDHVLMFFIAVIAGSLVTALFVNVLKKDITASPVLSETAPTSAPSEAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AANEIKQPIQSQKAEMSEFKKLTDIISPELIEPNLSGETSDDIIDELIQKLSRRGALLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
SGFKQAILNREQQGTTAIGMNIAIPHGKSEAVREPSVAFGIKRSGVDWNSLDGSEAKLIF
CCHHHHHHCCCCCCCEEEEEEEECCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCCCEEEE
MIAVPKESGGNQHLKILQMLSRKLMDDNYRERLLSVQTTEEAYKLLEEIE
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377