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The map label for this gene is appB

Identifier: 255767258

GI number: 255767258

Start: 1215243

End: 1216193

Strand: Direct

Name: appB

Synonym: BSU11390

Alternate gene names: 255767258

Gene position: 1215243-1216193 (Clockwise)

Preceding gene: 255767257

Following gene: 16078205

Centisome position: 28.83

GC content: 41.96

Gene sequence:

>951_bases
ATGGCTGCATATATTATCAGAAGAACCTTAATGTCTATCCCGATTTTATTGGGAATTACGATTTTATCATTTGTTATCAT
GAAAGCCGCGCCCGGAGATCCGATGACATTAATGATGGACCCGAAAATCAGCCAGGCTGACAGAGAGCAGTTTATTGAAA
AATACGGTCTGAATGATCCTCAGTACGTTCAATATTTAAAGTGGCTAGGCAACATGGTGCAGGGTGATTTCGGAACGTCT
ATTGTCAGAAAAGGAACGCCTGTTTCAGAATTGATTATGGCAAGACTACCCAATACGCTCCTGCTGATGCTGGTTTCAAC
GATTTTGGCTCTTATGATTTCTATTCCGTTCGGTGTCCTGTCGGCAAAGCGGCCGTATTCAAAAATCGACTATGGCATTA
CATTTACCTCGTTTATAGGCCTGGCTATTCCAAACTTTTGGTTTGGATTAATTTTGATTATGGTGTTATCGGTAAATCTC
GGCTGGTTTCCAACTGGGGGTGTCGAGACACTGAATACCGAATTCAATATATTTGACCGGATTCATCATTTAATTCTGCC
TGCATTCGTTCTAGCTACAGCTGACATGGCCGGACTGACGAGATACACGAGATCCAATATGCTTGATGTATTAAATCAAG
ATTATATTCGCACAGCGAGAGCAAAAGGATTTAAAGAGAACAGAGTGCTATTTAAACATGGATTGAGGAATGCATTATTA
CCCGTCATTACAATTTTCGGTTTGATGATTCCTTCATTTATCGGCGGATCTGTCGTTGTCGAACAAATTTTCACTTGGCC
GGGATTAGGAAAGCTGTTTGTCGATTCAGCATTCCAGCGCGATTATCCGGTTATCATGGCAATGACTGTGATCTCGGCGG
TTCTTGTCGTTGTCGGAAACCTCATTGCAGACATCCTTTACGCGATTGTTGATCCGCGGATCGAATATTAA

Upstream 100 bases:

>100_bases
TGAAAAATGGTGGCTTGCAAAATAAATTTCCCTTAAAGGGGAGGGAGAGCGTTTTCTCCCTCTTCTGTTTTTAATAAGGA
AAGTAAAGGGGGACAAGTAT

Downstream 100 bases:

>100_bases
AAGGAGGGAAACAGAGATGTCAGAGCTGCAAACAACTCCCTCGCCGGAGATCAGGCTGAAAGAAAATATTTCGAAAAAGC
CAGAAACAATGACAAAGATT

Product: oligopeptide ABC transporter permease

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAAYIIRRTLMSIPILLGITILSFVIMKAAPGDPMTLMMDPKISQADREQFIEKYGLNDPQYVQYLKWLGNMVQGDFGTS
IVRKGTPVSELIMARLPNTLLLMLVSTILALMISIPFGVLSAKRPYSKIDYGITFTSFIGLAIPNFWFGLILIMVLSVNL
GWFPTGGVETLNTEFNIFDRIHHLILPAFVLATADMAGLTRYTRSNMLDVLNQDYIRTARAKGFKENRVLFKHGLRNALL
PVITIFGLMIPSFIGGSVVVEQIFTWPGLGKLFVDSAFQRDYPVIMAMTVISAVLVVVGNLIADILYAIVDPRIEY

Sequences:

>Translated_316_residues
MAAYIIRRTLMSIPILLGITILSFVIMKAAPGDPMTLMMDPKISQADREQFIEKYGLNDPQYVQYLKWLGNMVQGDFGTS
IVRKGTPVSELIMARLPNTLLLMLVSTILALMISIPFGVLSAKRPYSKIDYGITFTSFIGLAIPNFWFGLILIMVLSVNL
GWFPTGGVETLNTEFNIFDRIHHLILPAFVLATADMAGLTRYTRSNMLDVLNQDYIRTARAKGFKENRVLFKHGLRNALL
PVITIFGLMIPSFIGGSVVVEQIFTWPGLGKLFVDSAFQRDYPVIMAMTVISAVLVVVGNLIADILYAIVDPRIEY
>Mature_315_residues
AAYIIRRTLMSIPILLGITILSFVIMKAAPGDPMTLMMDPKISQADREQFIEKYGLNDPQYVQYLKWLGNMVQGDFGTSI
VRKGTPVSELIMARLPNTLLLMLVSTILALMISIPFGVLSAKRPYSKIDYGITFTSFIGLAIPNFWFGLILIMVLSVNLG
WFPTGGVETLNTEFNIFDRIHHLILPAFVLATADMAGLTRYTRSNMLDVLNQDYIRTARAKGFKENRVLFKHGLRNALLP
VITIFGLMIPSFIGGSVVVEQIFTWPGLGKLFVDSAFQRDYPVIMAMTVISAVLVVVGNLIADILYAIVDPRIEY

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1789965, Length=338, Percent_Identity=36.9822485207101, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1787053, Length=319, Percent_Identity=40.1253918495298, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1787497, Length=321, Percent_Identity=36.4485981308411, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1789888, Length=322, Percent_Identity=36.3354037267081, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1787761, Length=327, Percent_Identity=35.1681957186544, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788504, Length=362, Percent_Identity=31.2154696132597, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787550, Length=269, Percent_Identity=29.7397769516729, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APPB_BACSU (P42062)

Other databases:

- EMBL:   U20909
- EMBL:   AL009126
- PIR:   I40546
- RefSeq:   NP_389021.2
- ProteinModelPortal:   P42062
- EnsemblBacteria:   EBBACT00000000884
- GeneID:   936400
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11390
- NMPDR:   fig|224308.1.peg.1140
- GenoList:   BSU11390
- GeneTree:   EBGT00050000000642
- HOGENOM:   HBG724229
- ProtClustDB:   CLSK887058
- BioCyc:   BSUB:BSU11390-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 35279; Mature: 35147

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe48b6c0)-; HASH(0xe53b258)-; HASH(0xe03b188)-; HASH(0xe538754)-; HASH(0xe5439c8)-; HASH(0xe4f32a4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYIIRRTLMSIPILLGITILSFVIMKAAPGDPMTLMMDPKISQADREQFIEKYGLNDP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCH
QYVQYLKWLGNMVQGDFGTSIVRKGTPVSELIMARLPNTLLLMLVSTILALMISIPFGVL
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SAKRPYSKIDYGITFTSFIGLAIPNFWFGLILIMVLSVNLGWFPTGGVETLNTEFNIFDR
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHH
IHHLILPAFVLATADMAGLTRYTRSNMLDVLNQDYIRTARAKGFKENRVLFKHGLRNALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PVITIFGLMIPSFIGGSVVVEQIFTWPGLGKLFVDSAFQRDYPVIMAMTVISAVLVVVGN
HHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LIADILYAIVDPRIEY
HHHHHHHHHHCCCCCH
>Mature Secondary Structure 
AAYIIRRTLMSIPILLGITILSFVIMKAAPGDPMTLMMDPKISQADREQFIEKYGLNDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCH
QYVQYLKWLGNMVQGDFGTSIVRKGTPVSELIMARLPNTLLLMLVSTILALMISIPFGVL
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SAKRPYSKIDYGITFTSFIGLAIPNFWFGLILIMVLSVNLGWFPTGGVETLNTEFNIFDR
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHH
IHHLILPAFVLATADMAGLTRYTRSNMLDVLNQDYIRTARAKGFKENRVLFKHGLRNALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PVITIFGLMIPSFIGGSVVVEQIFTWPGLGKLFVDSAFQRDYPVIMAMTVISAVLVVVGN
HHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LIADILYAIVDPRIEY
HHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377