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The map label for this gene is lytE

Identifier: 255767222

GI number: 255767222

Start: 1018998

End: 1020002

Strand: Direct

Name: lytE

Synonym: BSU09420

Alternate gene names: 255767222

Gene position: 1018998-1020002 (Clockwise)

Preceding gene: 16078005

Following gene: 255767224

Centisome position: 24.17

GC content: 41.49

Gene sequence:

>1005_bases
ATGAAAAAGCAAATCATTACAGCTACGACAGCAGTTGTTTTAGGATCGACGTTATTTGCAGGAGCGGCATCTGCACAAAG
CATTAAGGTGAAAAAAGGCGACACGTTATGGGATCTTTCAAGAAAATACGACACAACGATCAGTAAAATTAAATCAGAGA
ACCACCTTCGTTCAGACATTATTTATGTGGGACAAACTTTATCGATTAACGGCAAATCTACAAGTTCAAAAAGCAGCAGT
TCTTCTTCCTCTTCTTCTACATACAAAGTAAAGAGCGGGGACAGCCTTTGGAAAATTTCAAAAAAATACGGCATGACAAT
CAATGAACTGAAGAAGCTGAATGGCTTAAAATCAGATTTGCTTCGTGTTGGACAAGTCCTGAAACTGAAAGGTTCAACTA
GTTCAAGCAGCTCCAGCTCATCAAAAGTGTCATCGTCTTCAACTTCTACTTATAAAGTGAAGAGCGGAGACAGCCTTTCT
AAAATTGCGAGCAAATACGGCACTACGGTTAGCAAATTAAAAAGCTTAAACGGCTTAAAATCAGATGTAATCTATGTCAA
CCAAGTATTGAAGGTGAAAGGAACAAGCACAAGCAGCTCAAAACCTGCTTCATCTTCATCGTCTTCAAGCAGCAAAACGT
CATCTACATCACTTAATGTGAGCAAGCTGGTTTCTGATGCAAAAGCGTTAGTCGGAACGCCATATAAATGGGGCGGAACG
ACAACTTCAGGCTTTGACTGCAGCGGATTCATTTGGTACGTACTGAATAAACAAACAAGTGTGGGCAGAACAAGCACTGC
AGGATACTGGAGTTCTATGAAGAGCATTGCCAGCCCGTCTGTTGGTGATTTCGTCTTCTTCACAACATATAAATCCGGCC
CTTCTCACATGGGGATTTACATTGGAAACAACAGTTTCATTCATGCAGGATCTGACGGCGTACAAATCAGCAGCCTGAAC
AACAGCTACTGGAAGCCTCGTTACCTCGGTGCGAAAAGATTCTAA

Upstream 100 bases:

>100_bases
GACTATTTCTGATGGGAATCTATCCTTATAATAGAAATCAATAAGATTAATTTAAAAAAAGAGATACATATATATAGAGT
TAACATTTGGGGAGGAAAAT

Downstream 100 bases:

>100_bases
TTTTTAGAGAAAACCCGTTCATTGGAACGGGTTTTTTTCATTAGACGGAGCACAGGCGTTTTGGTTGCTCCTAAAAATGA
AAATGTGATAAAAAATCCAA

Product: cell wall hydrolase

Products: NA

Alternate protein names: Cell wall-associated polypeptide CWBP33; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytE; Phosphatase-associated protein papQ

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKKQIITATTAVVLGSTLFAGAASAQSIKVKKGDTLWDLSRKYDTTISKIKSENHLRSDIIYVGQTLSINGKSTSSKSSS
SSSSSSTYKVKSGDSLWKISKKYGMTINELKKLNGLKSDLLRVGQVLKLKGSTSSSSSSSSKVSSSSTSTYKVKSGDSLS
KIASKYGTTVSKLKSLNGLKSDVIYVNQVLKVKGTSTSSSKPASSSSSSSSKTSSTSLNVSKLVSDAKALVGTPYKWGGT
TTSGFDCSGFIWYVLNKQTSVGRTSTAGYWSSMKSIASPSVGDFVFFTTYKSGPSHMGIYIGNNSFIHAGSDGVQISSLN
NSYWKPRYLGAKRF

Sequences:

>Translated_334_residues
MKKQIITATTAVVLGSTLFAGAASAQSIKVKKGDTLWDLSRKYDTTISKIKSENHLRSDIIYVGQTLSINGKSTSSKSSS
SSSSSSTYKVKSGDSLWKISKKYGMTINELKKLNGLKSDLLRVGQVLKLKGSTSSSSSSSSKVSSSSTSTYKVKSGDSLS
KIASKYGTTVSKLKSLNGLKSDVIYVNQVLKVKGTSTSSSKPASSSSSSSSKTSSTSLNVSKLVSDAKALVGTPYKWGGT
TTSGFDCSGFIWYVLNKQTSVGRTSTAGYWSSMKSIASPSVGDFVFFTTYKSGPSHMGIYIGNNSFIHAGSDGVQISSLN
NSYWKPRYLGAKRF
>Mature_334_residues
MKKQIITATTAVVLGSTLFAGAASAQSIKVKKGDTLWDLSRKYDTTISKIKSENHLRSDIIYVGQTLSINGKSTSSKSSS
SSSSSSTYKVKSGDSLWKISKKYGMTINELKKLNGLKSDLLRVGQVLKLKGSTSSSSSSSSKVSSSSTSTYKVKSGDSLS
KIASKYGTTVSKLKSLNGLKSDVIYVNQVLKVKGTSTSSSKPASSSSSSSSKTSSTSLNVSKLVSDAKALVGTPYKWGGT
TTSGFDCSGFIWYVLNKQTSVGRTSTAGYWSSMKSIASPSVGDFVFFTTYKSGPSHMGIYIGNNSFIHAGSDGVQISSLN
NSYWKPRYLGAKRF

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 LysM repeats

Homologues:

Organism=Escherichia coli, GI1787944, Length=214, Percent_Identity=30.8411214953271, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788501, Length=106, Percent_Identity=42.4528301886792, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786421, Length=112, Percent_Identity=35.7142857142857, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788001, Length=140, Percent_Identity=36.4285714285714, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LYTE_BACSU (P54421)

Other databases:

- EMBL:   U38819
- EMBL:   Y14082
- EMBL:   AL009126
- EMBL:   U05256
- PIR:   G69654
- RefSeq:   NP_388823.2
- ProteinModelPortal:   P54421
- MEROPS:   C40.003
- EnsemblBacteria:   EBBACT00000002574
- GeneID:   939269
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09420
- GenoList:   BSU09420
- GeneTree:   EBGT00070000031872
- HOGENOM:   HBG362075
- PhylomeDB:   P54421
- ProtClustDB:   CLSK2816770
- BioCyc:   BSUB:BSU09420-MONOMER
- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482
- SMART:   SM00257

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60

EC number: NA

Molecular weight: Translated: 35455; Mature: 35455

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQIITATTAVVLGSTLFAGAASAQSIKVKKGDTLWDLSRKYDTTISKIKSENHLRSDI
CCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHCCE
IYVGQTLSINGKSTSSKSSSSSSSSSTYKVKSGDSLWKISKKYGMTINELKKLNGLKSDL
EEECEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCHHHHHHHCCHHHHH
LRVGQVLKLKGSTSSSSSSSSKVSSSSTSTYKVKSGDSLSKIASKYGTTVSKLKSLNGLK
HHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHCCCH
SDVIYVNQVLKVKGTSTSSSKPASSSSSSSSKTSSTSLNVSKLVSDAKALVGTPYKWGGT
HHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
TTSGFDCSGFIWYVLNKQTSVGRTSTAGYWSSMKSIASPSVGDFVFFTTYKSGPSHMGIY
CCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEE
IGNNSFIHAGSDGVQISSLNNSYWKPRYLGAKRF
ECCCCEEEECCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKQIITATTAVVLGSTLFAGAASAQSIKVKKGDTLWDLSRKYDTTISKIKSENHLRSDI
CCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHCCE
IYVGQTLSINGKSTSSKSSSSSSSSSTYKVKSGDSLWKISKKYGMTINELKKLNGLKSDL
EEECEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCHHHHHHHCCHHHHH
LRVGQVLKLKGSTSSSSSSSSKVSSSSTSTYKVKSGDSLSKIASKYGTTVSKLKSLNGLK
HHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHCCCH
SDVIYVNQVLKVKGTSTSSSKPASSSSSSSSKTSSTSLNVSKLVSDAKALVGTPYKWGGT
HHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
TTSGFDCSGFIWYVLNKQTSVGRTSTAGYWSSMKSIASPSVGDFVFFTTYKSGPSHMGIY
CCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEE
IGNNSFIHAGSDGVQISSLNNSYWKPRYLGAKRF
ECCCCEEEECCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9457885; 9579061; 9384377; 8045898