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The map label for this gene is citA

Identifier: 255767224

GI number: 255767224

Start: 1021057

End: 1022157

Strand: Direct

Name: citA

Synonym: BSU09440

Alternate gene names: 255767224

Gene position: 1021057-1022157 (Clockwise)

Preceding gene: 255767222

Following gene: 16078010

Centisome position: 24.22

GC content: 46.14

Gene sequence:

>1101_bases
ATGGTACATTACGGATTAAAGGGAATTACATGTGTAGAAACATCCATCAGCCATATTGATGGTGAAAAAGGGCGTCTGAT
TTACAGAGGACATCATGCAAAAGACATCGCACTGAATCACAGCTTTGAAGAAGCGGCTTATTTGATTTTATTTGGAAAAC
TGCCTTCAACTGAAGAATTGCAGGTATTTAAAGATAAGCTGGCTGCGGAACGGAATCTTCCGGAACATATTGAGCGCTTG
ATTCAATCACTGCCAAACAACATGGACGATATGTCGGTGCTGAGAACGGTTGTTTCAGCATTGGGGGAAAATACGTACAC
ATTCCATCCGAAAACAGAGGAAGCAATCCGGCTGATTGCAATCACGCCTTCTATCATTGCATACAGAAAAAGATGGACGC
GCGGTGAACAAGCAATTGCACCTTCTTCCCAGTACGGACATGTCGAAAACTACTATTATATGCTCACAGGTGAACAGCCT
TCAGAGGCAAAAAAGAAAGCGCTTGAAACCTATATGATTCTGGCCACGGAGCACGGCATGAACGCATCGACTTTTTCGGC
ACGAGTCACGTTATCTACAGAGAGTGATTTAGTGTCAGCCGTCACGGCTGCTCTCGGTACGATGAAAGGTCCGCTGCACG
GAGGTGCTCCTTCTGCGGTGACAAAAATGCTTGAAGACATTGGAGAAAAGGAACATGCTGAAGCATATCTGAAAGAAAAA
CTCGAAAAAGGAGAGCGCTTGATGGGATTCGGCCACAGGGTGTACAAAACAAAAGACCCGCGGGCAGAAGCTTTGAGACA
GAAAGCAGAAGAGGTGGCCGGCAATGACCGTGATCTTGATCTTGCGCTGCATGTAGAAGCAGAAGCGATTCGTTTGCTAG
AAATCTACAAACCGGGACGAAAGCTGTACACAAATGTTGAATTTTACGCAGCTGCAGTGATGAGGGCCATCGATTTTGAC
GATGAATTGTTTACTCCGACCTTTTCAGCAAGCCGAATGGTAGGGTGGTGCGCCCATGTACTGGAACAGGCAGAAAACAA
CATGATTTTCCGCCCGTCAGCGCAATATACCGGTGCCATTCCCGAGGAAGTTCTTTCATAA

Upstream 100 bases:

>100_bases
TCCCTCTGATTAATATTTTTAATTAATTCCCTTTAAAATATTGATTATTTTTTAAATATTATATTTACTATAATAACAGA
AAAGGATAGGGGGAATACAA

Downstream 100 bases:

>100_bases
CATATTTTGGCGTTTATTCATTTCTGTCGTGTGGTAACGTTCAGTATCAACCACATGTCTAGGAGTGATGAATGTGAACC
CAATGGACAGACAAACAGAA

Product: citrate synthase I

Products: NA

Alternate protein names: Citrate synthase I

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MVHYGLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEELQVFKDKLAAERNLPEHIERL
IQSLPNNMDDMSVLRTVVSALGENTYTFHPKTEEAIRLIAITPSIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQP
SEAKKKALETYMILATEHGMNASTFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEK
LEKGERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGRKLYTNVEFYAAAVMRAIDFD
DELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAIPEEVLS

Sequences:

>Translated_366_residues
MVHYGLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEELQVFKDKLAAERNLPEHIERL
IQSLPNNMDDMSVLRTVVSALGENTYTFHPKTEEAIRLIAITPSIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQP
SEAKKKALETYMILATEHGMNASTFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEK
LEKGERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGRKLYTNVEFYAAAVMRAIDFD
DELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAIPEEVLS
>Mature_366_residues
MVHYGLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEELQVFKDKLAAERNLPEHIERL
IQSLPNNMDDMSVLRTVVSALGENTYTFHPKTEEAIRLIAITPSIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQP
SEAKKKALETYMILATEHGMNASTFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEK
LEKGERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGRKLYTNVEFYAAAVMRAIDFD
DELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAIPEEVLS

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Homo sapiens, GI38327625, Length=321, Percent_Identity=24.2990654205607, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786527, Length=364, Percent_Identity=33.2417582417582, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786939, Length=371, Percent_Identity=31.8059299191375, Blast_Score=192, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17555174, Length=374, Percent_Identity=22.192513368984, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=389, Percent_Identity=23.9074550128535, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319850, Length=395, Percent_Identity=23.5443037974684, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640126, Length=390, Percent_Identity=21.7948717948718, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640124, Length=390, Percent_Identity=21.7948717948718, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356863, Length=365, Percent_Identity=22.4657534246575, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CISY_BACSU (P39119)

Other databases:

- EMBL:   U05256
- EMBL:   Y14082
- EMBL:   AL009126
- PIR:   I40380
- RefSeq:   NP_388825.2
- PDB:   2C6X
- PDBsum:   2C6X
- ProteinModelPortal:   P39119
- SMR:   P39119
- EnsemblBacteria:   EBBACT00000003430
- GeneID:   936270
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09440
- NMPDR:   fig|224308.1.peg.944
- GenoList:   BSU09440
- GeneTree:   EBGT00050000001635
- HOGENOM:   HBG649489
- ProtClustDB:   CLSK886958
- BioCyc:   BSUB:BSU09440-MONOMER
- BRENDA:   2.3.3.1
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.1

Molecular weight: Translated: 40952; Mature: 40952

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 252-252 ACT_SITE 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHYGLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEEL
CCCCCCCCEEEHHHHHHHCCCCCCCEEEECCCCCEEEECCCHHHEEEEEEECCCCCCHHH
QVFKDKLAAERNLPEHIERLIQSLPNNMDDMSVLRTVVSALGENTYTFHPKTEEAIRLIA
HHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHEEEEE
ITPSIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQPSEAKKKALETYMILATEHGM
ECHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHEEEEECCCC
NASTFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEK
CCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LEKGERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGR
HHHCCHHHHCCCHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHCCCH
KLYTNVEFYAAAVMRAIDFDDELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAI
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PEEVLS
HHHHCC
>Mature Secondary Structure
MVHYGLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEEL
CCCCCCCCEEEHHHHHHHCCCCCCCEEEECCCCCEEEECCCHHHEEEEEEECCCCCCHHH
QVFKDKLAAERNLPEHIERLIQSLPNNMDDMSVLRTVVSALGENTYTFHPKTEEAIRLIA
HHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHEEEEE
ITPSIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQPSEAKKKALETYMILATEHGM
ECHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHEEEEECCCC
NASTFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEK
CCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
LEKGERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGR
HHHCCHHHHCCCHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHCCCH
KLYTNVEFYAAAVMRAIDFDDELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAI
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PEEVLS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377