Click here to switch to the map view.

The map label for this gene is phoB

Identifier: 255767150

GI number: 255767150

Start: 620097

End: 621485

Strand: Reverse

Name: phoB

Synonym: BSU05740

Alternate gene names: 255767150

Gene position: 621485-620097 (Counterclockwise)

Preceding gene: 16077643

Following gene: 16077640

Centisome position: 14.74

GC content: 44.28

Gene sequence:

>1389_bases
TTGAAAAAATTCCCGAAGAAATTACTGCCTATCGCGGTTTTATCATCAATTGCGTTCAGCAGCTTAGCCAGCGGCAGTGT
GCCTGAAGCCAGCGCCCAGGAAAAGAAAAAGGGGAACCAAGACGAAATTAAAAATGTTATTGTTCTGATTGGTGATGGTA
TGGGTGTGTCTTATACGTCTGCTTATCGATACTTAAAGGATAATAAAAAGACAAAAGTTGTGGAGCCTACGGCTTTTGAT
CAGTATCTTGTCGGCCAGCAAACAACTTACCCCGATGATCCTGAACAAAATGTAACGGATTCTGCAGCGGCGGCAACGGC
TATGTCTGCGGGAATTAAAACATACAATAATGCCATAGCCGTTGATAACGACGGGTCAGAAGCCAAAACGGTGCTTGAAG
CTGCGAAAGAAAAAGGAAAAGCAACAGGACTTGTGGCCACTTCAGAAATTACACACGCCACTCCGGCCTCTTTTGGTTCA
CACGACCACAGCCGAAAAAATATGAACTCAATTGCGGATGATTATTTTGATGAGATGGTCAACGGCAAGCACAAAATTGA
TGTCCTTCTAGGCGGAGGAAAGAGCAATTTTGACCGTAAGGACCGTAATTTGATTAAGGAATTCAAAAAGGCGGGGTACA
GCTACGTGGATGATCGCAAGGACATGCTGAAAAACAAAGACAGCCAAGTTCTCGGCCTATTCGCTGACGGCGGCCTGCCT
AAAAAGATTGACCGTACGAAGGATATTCCATCACTTAAAGATATGACAAACACAGCCATTAAAAAATTAAACAAGGATAA
AGACGGATTCTTCCTCATGGTGGAAGGCAGCCAGATTGACTGGGCGGGCCATGACAATGATATCGTTGGCGCCATGAGCG
AGATGGAAGACTTCGAACAAGCTTATAAGGCGGCTATTGATTTCGCTAAAAAAGACAAGCACACATTGGTTGTCGCAACG
GCTGACCACTCAACCGGCGGATATTCCATCGGTGCGGACGGCATCTACAACTGGTTCAGTGAACCCATTAAAGCCGCAAA
GCGCACACCAGACTTTATGGCAGAAAAGATCGCTGACGGCGCGGATGTTGAAAAAACGTTGAAAACATATATCGATCAAA
AGAAATTGGCTTTAACAAAAGCCGAAATTCAATCTGTTGAAGAAGCGGCAAAATCGAAGGAAGTACTGGATATCGATAAC
GCAATCGAGAACATCTTTAATAAACGCTCTCACACTGGATGGACAACAGGCGGACATACCGGTGAAGATGTTCCCGTCTA
TGCATACGGTCCGTCTAGTGAGACATTTGCCGGCCAAATCGACAATACAGAAATCGCCAAAAATGTTTTCAAGGCTTTAC
AATACAACATTAAGATTAACGATAAGTAA

Upstream 100 bases:

>100_bases
TTGTAAATCCAATCTTTAAAATCGATTAATACTAGCTTAACAGTTTAAAAATATAATTGGGTTGTCATTGAGATTCATCT
ATATTTAGGAGGTTATCCAG

Downstream 100 bases:

>100_bases
AAGAATACAAGGTGTCCCGTTGCTTGTATGAAGTAACGGGATCTCTTTTTAATGGAGGAATCATCATGAGAAGAATTCTT
TCAATTCTCGTTTTTGCTAT

Product: alkaline phosphatase III

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTSAYRYLKDNKKTKVVEPTAFD
QYLVGQQTTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIAVDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGS
HDHSRKNMNSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLP
KKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVAT
ADHSTGGYSIGADGIYNWFSEPIKAAKRTPDFMAEKIADGADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDIDN
AIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK

Sequences:

>Translated_462_residues
MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTSAYRYLKDNKKTKVVEPTAFD
QYLVGQQTTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIAVDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGS
HDHSRKNMNSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLP
KKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVAT
ADHSTGGYSIGADGIYNWFSEPIKAAKRTPDFMAEKIADGADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDIDN
AIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK
>Mature_462_residues
MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTSAYRYLKDNKKTKVVEPTAFD
QYLVGQQTTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIAVDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGS
HDHSRKNMNSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLP
KKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVAT
ADHSTGGYSIGADGIYNWFSEPIKAAKRTPDFMAEKIADGADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDIDN
AIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family

Homologues:

Organism=Homo sapiens, GI94721246, Length=470, Percent_Identity=30, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI157266296, Length=470, Percent_Identity=30.4255319148936, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI157266292, Length=360, Percent_Identity=31.1111111111111, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI116734717, Length=463, Percent_Identity=26.9978401727862, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI294660770, Length=447, Percent_Identity=26.1744966442953, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI294660772, Length=313, Percent_Identity=27.7955271565495, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI48994877, Length=335, Percent_Identity=34.9253731343284, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320689, Length=501, Percent_Identity=34.3313373253493, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI18859923, Length=471, Percent_Identity=28.4501061571125, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355981, Length=454, Percent_Identity=29.5154185022026, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24651554, Length=487, Percent_Identity=29.5687885010267, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21355151, Length=457, Percent_Identity=27.1334792122538, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355149, Length=469, Percent_Identity=28.1449893390192, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI85725200, Length=483, Percent_Identity=27.7432712215321, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI85815833, Length=483, Percent_Identity=27.7432712215321, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921912, Length=476, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI21358067, Length=468, Percent_Identity=29.0598290598291, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24651556, Length=468, Percent_Identity=29.0598290598291, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24657835, Length=461, Percent_Identity=27.114967462039, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24657842, Length=461, Percent_Identity=27.114967462039, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24657827, Length=464, Percent_Identity=27.1551724137931, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585213, Length=350, Percent_Identity=28.2857142857143, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PPB3_BACSU (P19405)

Other databases:

- EMBL:   D88802
- EMBL:   AL009126
- EMBL:   M33634
- PIR:   C69676
- RefSeq:   NP_388455.2
- ProteinModelPortal:   P19405
- SMR:   P19405
- EnsemblBacteria:   EBBACT00000002507
- GeneID:   938004
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU05740
- NMPDR:   fig|224308.1.peg.574
- GenoList:   BSU05740
- GeneTree:   EBGT00050000002942
- HOGENOM:   HBG617166
- PhylomeDB:   P19405
- ProtClustDB:   CLSK886792
- BioCyc:   BSUB:BSU05740-MONOMER
- BRENDA:   3.1.3.1
- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850
- Gene3D:   G3DSA:3.40.720.10
- PRINTS:   PR00113
- SMART:   SM00098

Pfam domain/function: PF00245 Alk_phosphatase; SSF53649 Alkaline_phosphatase_core

EC number: =3.1.3.1

Molecular weight: Translated: 50494; Mature: 50494

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: ACT_SITE 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCEEEEEEECCCCCHHHH
AYRYLKDNKKTKVVEPTAFDQYLVGQQTTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIA
HHHHHHCCCCCEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEE
VDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGSHDHSRKNMNSIADDYFDEMV
ECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLP
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
KKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQ
CHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHH
AYKAAIDFAKKDKHTLVVATADHSTGGYSIGADGIYNWFSEPIKAAKRTPDFMAEKIADG
HHHHHHHHHCCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
ADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDIDNAIENIFNKRSHTGWTTGGHT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
GEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHEECEEECCC
>Mature Secondary Structure
MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCEEEEEEECCCCCHHHH
AYRYLKDNKKTKVVEPTAFDQYLVGQQTTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIA
HHHHHHCCCCCEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEE
VDNDGSEAKTVLEAAKEKGKATGLVATSEITHATPASFGSHDHSRKNMNSIADDYFDEMV
ECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDRKDMLKNKDSQVLGLFADGGLP
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
KKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQ
CHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHH
AYKAAIDFAKKDKHTLVVATADHSTGGYSIGADGIYNWFSEPIKAAKRTPDFMAEKIADG
HHHHHHHHHCCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
ADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDIDNAIENIFNKRSHTGWTTGGHT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
GEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301