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The map label for this gene is pepT

Identifier: 16080943

GI number: 16080943

Start: 3995075

End: 3996307

Strand: Direct

Name: pepT

Synonym: BSU38920

Alternate gene names: 16080943

Gene position: 3995075-3996307 (Clockwise)

Preceding gene: 16080942

Following gene: 16080944

Centisome position: 94.77

GC content: 47.28

Gene sequence:

>1233_bases
ATGAAAGAAGAAATCATTGAACGGTTTACTACATATGTAAAAGTCGATACACAGTCCGATGAATCCGTTGACACTTGTCC
TTCCACACCCGGCCAATTGACATTAGGAAACATGCTAGTTGATGAATTGAAGTCCATCGGCATGCAGGATGCAGCCATTG
ATGAAAACGGCTATGTCATGGCAACTCTTCCCTCCAATACTGAAAAAGACGTGCCGACAATCGGCTTTCTCGCTCACGTC
GACACCGCTACAGACTTTACCGGCAAAAATGTGAATCCGCAAATCATCGAAAGCTATGATGGAAAAGACATCGTTCTTAA
CGAACAGCTGCAGGTCACCCTGTCACCTGATCAATTCCCTGAGCTGTCCGGCTATAAGGGCCATACACTCATCACCACAG
ACGGCACCACCCTTCTCGGCGCAGACAATAAGGCCGGTATCGCTGAAATCATGACAGCCATGGATTACCTCATCAAACAC
CCTGAAATCAAGCACGGCACAATCAGAGTCGCCTTTACGCCTGATGAAGAAATCGGAAGAGGGCCGCACAAATTTGATGT
GAAACGGTTCAATGCATCCTTTGCCTACACGGTTGACGGCGGGCCGCTCGGCGAGCTTGAATATGAAAGCTTCAATGCCG
CTGCCGCAAAAATTACGATTAAAGGCAACAACGTACATCCCGGAACAGCTAAAGGAAAAATGATCAACTCTGCGAAAATC
GCGATGAAGCTGAACAGCCTGCTGCCGGCAGACGAAGCGCCTGAATATACGGAAGGTTATGAAGGCTTCTACCACCTGCT
GTCGATTCAAGGAGACGTAGAGGAAACAAAGCTCCACTACATTATCAGAGACTTTGACAAAGAGAATTTCCAAAACAGAA
AAGAAACAATGAAGCGTGCCGTTGAAGAACTTCAAAACGAATACGGGCAAGACCGGATTCTGCTGGATATGAACGACCAA
TACTACAACATGAGAGAGAAAATTGAACCTGTCATCGAAATCGTCAACATTGCCAAGCAAGCGATGGAAAACCTCGGCAT
CGAACCGAAGATTTCCCCCATCCGCGGCGGAACCGACGGATCACAGCTGTCCTACATGGGGCTTCCGACGCCAAACATCT
TTACAGGCGGGGAAAACTTCCACGGCAAATTTGAATACATCTCAGTTGACAACATGGTCAAAGCCGTAAATGTCATCGTT
GAGATTGCAAAGCAGTTTGAAGCGCAAGCATAA

Upstream 100 bases:

>100_bases
GACCGGACACAGGCAGTCGTTTTTGCCATTCGAAACGGTATTGTGCAATAGAAATCTCTCATCCCGCCATGCTAAAATAA
GACAAAGGAGATGATTGGAA

Downstream 100 bases:

>100_bases
CGCCAAAAGCCAGTCCAAAAAAGGACTGGCTTTTTTGTGTGAAAATGAAACATTTTAAACACGTCCTCCGTAAAACAAGT
ATCAACAAATGAGGAGGAAA

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAIDENGYVMATLPSNTEKDVPTIGFLAHV
DTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKH
PEIKHGTIRVAFTPDEEIGRGPHKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKI
AMKLNSLLPADEAPEYTEGYEGFYHLLSIQGDVEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMNDQ
YYNMREKIEPVIEIVNIAKQAMENLGIEPKISPIRGGTDGSQLSYMGLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIV
EIAKQFEAQA

Sequences:

>Translated_410_residues
MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAIDENGYVMATLPSNTEKDVPTIGFLAHV
DTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKH
PEIKHGTIRVAFTPDEEIGRGPHKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKI
AMKLNSLLPADEAPEYTEGYEGFYHLLSIQGDVEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMNDQ
YYNMREKIEPVIEIVNIAKQAMENLGIEPKISPIRGGTDGSQLSYMGLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIV
EIAKQFEAQA
>Mature_410_residues
MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAIDENGYVMATLPSNTEKDVPTIGFLAHV
DTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKH
PEIKHGTIRVAFTPDEEIGRGPHKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKI
AMKLNSLLPADEAPEYTEGYEGFYHLLSIQGDVEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMNDQ
YYNMREKIEPVIEIVNIAKQAMENLGIEPKISPIRGGTDGSQLSYMGLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIV
EIAKQFEAQA

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=403, Percent_Identity=55.0868486352357, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_BACSU (P55179)

Other databases:

- EMBL:   X99339
- EMBL:   D83026
- EMBL:   AL009126
- PIR:   H69674
- RefSeq:   NP_391771.1
- ProteinModelPortal:   P55179
- SMR:   P55179
- MEROPS:   M20.003
- EnsemblBacteria:   EBBACT00000001413
- GeneID:   937428
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38920
- NMPDR:   fig|224308.1.peg.3897
- GenoList:   BSU38920
- GeneTree:   EBGT00050000000947
- HOGENOM:   HBG288638
- OMA:   HMDTAPD
- PhylomeDB:   P55179
- ProtClustDB:   PRK05469
- BioCyc:   BSUB:BSU38920-MONOMER
- BRENDA:   3.4.11.4
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 45510; Mature: 45510

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 81-81 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAIDENGYVM
CHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCEECCCCEEE
ATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFP
EECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCEEEECCEEEEEECCCCCC
ELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIGR
CCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPHKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKI
CCCEEEHEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCHHE
AMKLNSLLPADEAPEYTEGYEGFYHLLSIQGDVEETKLHYIIRDFDKENFQNRKETMKRA
EEEHHHCCCCCCCCCHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
VEELQNEYGQDRILLDMNDQYYNMREKIEPVIEIVNIAKQAMENLGIEPKISPIRGGTDG
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SQLSYMGLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA
CCEEEECCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAIDENGYVM
CHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCEECCCCEEE
ATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFP
EECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCEEEECCEEEEEECCCCCC
ELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIGR
CCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPHKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKI
CCCEEEHEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCHHE
AMKLNSLLPADEAPEYTEGYEGFYHLLSIQGDVEETKLHYIIRDFDKENFQNRKETMKRA
EEEHHHCCCCCCCCCHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
VEELQNEYGQDRILLDMNDQYYNMREKIEPVIEIVNIAKQAMENLGIEPKISPIRGGTDG
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SQLSYMGLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA
CCEEEECCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8978088; 8969509; 9384377; 10987140