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The map label for this gene is ywaD

Identifier: 16080898

GI number: 16080898

Start: 3948555

End: 3949922

Strand: Direct

Name: ywaD

Synonym: BSU38470

Alternate gene names: 16080898

Gene position: 3948555-3949922 (Clockwise)

Preceding gene: 16080897

Following gene: 255767815

Centisome position: 93.67

GC content: 47.66

Gene sequence:

>1368_bases
ATGAAAAAGCTTTTGACTGTCATGACGATGGCTGTTTTAACTGCCGGCACACTGCTCTTGCCGGCACAGAGTGTCACCCC
TGCCGCGCACGCTGTACAAATATCAAATAGCGAGCGCGAGCTTCCATTCAAAGCAAAACATGCGTACTCTACCATTTCTC
AGCTAAGTGAAGCAATCGGCCCCAGAATAGCCGGAACTGCAGCTGAAAAAAAGAGTGCCCTATTGATCGCCTCATCAATG
AGAAAATTAAAGCTTGATGTGAAGGTTCAACGATTCAACATTCCTGACCGGCTTGAGGGTACACTGTCTTCAGCAGGACG
CGATATTCTTCTCCAAGCGGCGTCGGGCTCAGCTCCGACTGAGGAACAAGGGCTGACGGCCCCGCTTTACAATGCGGGAT
TGGGCTATCAAAAGGACTTTACCGCTGACGCCAAGGGCAAAATCGCCTTGATTTCCAGAGGAGACCTGACTTATTACGAG
AAAGCCAAAAATGCCGAAGCCGCCGGAGCGAAAGCTGTCATCATTTATAACAACAAAGAAAGCCTCGTGCCTATGACGCC
AAACCTGTCGGGAAATAAAGTCGGCATTCCGGTCGTCGGCATTAAGAAAGAAGACGGCGAAGCACTTACCCAGCAAAAAG
AAGCCACCTTAAAACTAAAAGCATTCACAAACCAAACCTCCCAGAATATCATCGGAATCAAAAAACCAAAGAACATCAAA
CATCCAGACATTGTGTACGTGACCGCCCATTACGACAGTGTTCCTTTTTCGCCCGGAGCAAATGACAACGGCTCAGGTAC
CTCTGTTATGCTGGAGATGGCGCGTGTCTTAAAAAGCGTTCCATCTGATAAAGAAATCCGCTTTATCGCTTTCGGCGCCG
AAGAGCTCGGCCTGCTCGGCTCCTCTCACTATGTAGATCATCTATCGGAAAAAGAGCTGAAACGAAGCGAAGTGAACTTC
AACTTAGATATGGTAGGCACAAGCTGGGAAAAAGCGTCTGAGCTGTATGTCAACACATTGGATGGCCAATCTAACTATGT
TTGGGAATCCAGCCGTACGGCTGCTGAAAAAATTGGGTTTGACAGCCTGTCTCTGACACAGGGAGGATCATCTGATCATG
TGCCATTCCACGAAGCCGGCATTGATTCCGCTAACTTTATTTGGGGAGACCCGGAAACAGAAGAAGTTGAGCCGTGGTAT
CATACCCCTGAAGACTCGATCGAACATATCAGCAAAGAACGCCTCCAGCAAGCAGGCGATCTTGTGACAGCCGCTGTCTA
TGAGGCTGTGAAAAAAGAGAAGAAACCGAAAACCATTAAGAAACAAATGAAGGCGAAAGCATCTGACATTTTTGAAGATA
TCAAATAA

Upstream 100 bases:

>100_bases
TAAAGCACTGTCTTTTCAAAAACGCTCTCCATCTTGCAGGAAACTCCTTTAGCATCCCGAAATATTCCCCAACCATACAT
TTAGGGGAGGTTTATTTTTG

Downstream 100 bases:

>100_bases
AAAAAGACGGCACTTGGGTGCCGTCTTTTTTAATCCACTTCTTTCTTAATCCCCAGCATTTTCTCATCTAGGTAATGGGC
AATCTCCGCGGCTTCTTTCA

Product: double-zinc aminopeptidase

Products: NA

Alternate protein names: Arginyl aminopeptidase; BSAP; Leucyl aminopeptidase

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELPFKAKHAYSTISQLSEAIGPRIAGTAAEKKSALLIASSM
RKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPTEEQGLTAPLYNAGLGYQKDFTADAKGKIALISRGDLTYYE
KAKNAEAAGAKAVIIYNNKESLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIK
HPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLGSSHYVDHLSEKELKRSEVNF
NLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGFDSLSLTQGGSSDHVPFHEAGIDSANFIWGDPETEEVEPWY
HTPEDSIEHISKERLQQAGDLVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK

Sequences:

>Translated_455_residues
MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELPFKAKHAYSTISQLSEAIGPRIAGTAAEKKSALLIASSM
RKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPTEEQGLTAPLYNAGLGYQKDFTADAKGKIALISRGDLTYYE
KAKNAEAAGAKAVIIYNNKESLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIK
HPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLGSSHYVDHLSEKELKRSEVNF
NLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGFDSLSLTQGGSSDHVPFHEAGIDSANFIWGDPETEEVEPWY
HTPEDSIEHISKERLQQAGDLVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK
>Mature_455_residues
MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELPFKAKHAYSTISQLSEAIGPRIAGTAAEKKSALLIASSM
RKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPTEEQGLTAPLYNAGLGYQKDFTADAKGKIALISRGDLTYYE
KAKNAEAAGAKAVIIYNNKESLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIK
HPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLGSSHYVDHLSEKELKRSEVNF
NLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGFDSLSLTQGGSSDHVPFHEAGIDSANFIWGDPETEEVEPWY
HTPEDSIEHISKERLQQAGDLVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK

Specific function: Catalyzes the hydrolysis of a range of N-terminal amino acids

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28B subfamily

Homologues:

Organism=Saccharomyces cerevisiae, GI6319763, Length=195, Percent_Identity=34.3589743589744, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BSAP_BACSU (P25152)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- EMBL:   X52480
- PIR:   S39663
- RefSeq:   NP_391726.1
- ProteinModelPortal:   P25152
- MEROPS:   M28.020
- EnsemblBacteria:   EBBACT00000001166
- GeneID:   937350
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38470
- NMPDR:   fig|224308.1.peg.3852
- GenoList:   BSU38470
- GeneTree:   EBGT00050000000294
- HOGENOM:   HBG574629
- OMA:   QDNVLEN
- ProtClustDB:   CLSK2300095
- BioCyc:   BSUB:BSU38470-MONOMER
- BRENDA:   3.4.11.10
- BRENDA:   3.4.11.6
- GO:   GO:0006508
- InterPro:   IPR007484
- InterPro:   IPR003137

Pfam domain/function: PF02225 PA; PF04389 Peptidase_M28

EC number: =3.4.11.6; =3.4.11.10

Molecular weight: Translated: 49451; Mature: 49451

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELPFKAKHAYSTISQLSEAIG
CHHHHHHHHHHHHHCCHHEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
PRIAGTAAEKKSALLIASSMRKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPT
CCEECCCHHHCCEEEEEHHHHHEEEEEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCC
EEQGLTAPLYNAGLGYQKDFTADAKGKIALISRGDLTYYEKAKNAEAAGAKAVIIYNNKE
HHCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEECCCC
SLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIK
CEEEECCCCCCCEECCEEEEEECCCCHHHHHHHHHEEEEEEECCCCCCCEEEECCCCCCC
HPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLG
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHCCC
SSHYVDHLSEKELKRSEVNFNLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGF
CCHHHHHHHHHHHHHHHCCEEEEEECCCHHHCCEEEEEEECCCCCEEECCCCHHHHHCCC
DSLSLTQGGSSDHVPFHEAGIDSANFIWGDPETEEVEPWYHTPEDSIEHISKERLQQAGD
CCEEECCCCCCCCCCHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLLTVMTMAVLTAGTLLLPAQSVTPAAHAVQISNSERELPFKAKHAYSTISQLSEAIG
CHHHHHHHHHHHHHCCHHEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
PRIAGTAAEKKSALLIASSMRKLKLDVKVQRFNIPDRLEGTLSSAGRDILLQAASGSAPT
CCEECCCHHHCCEEEEEHHHHHEEEEEEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCC
EEQGLTAPLYNAGLGYQKDFTADAKGKIALISRGDLTYYEKAKNAEAAGAKAVIIYNNKE
HHCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEECCCC
SLVPMTPNLSGNKVGIPVVGIKKEDGEALTQQKEATLKLKAFTNQTSQNIIGIKKPKNIK
CEEEECCCCCCCEECCEEEEEECCCCHHHHHHHHHEEEEEEECCCCCCCEEEECCCCCCC
HPDIVYVTAHYDSVPFSPGANDNGSGTSVMLEMARVLKSVPSDKEIRFIAFGAEELGLLG
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHCCC
SSHYVDHLSEKELKRSEVNFNLDMVGTSWEKASELYVNTLDGQSNYVWESSRTAAEKIGF
CCHHHHHHHHHHHHHHHCCEEEEEECCCHHHCCEEEEEEECCCCCEEECCCCHHHHHCCC
DSLSLTQGGSSDHVPFHEAGIDSANFIWGDPETEEVEPWYHTPEDSIEHISKERLQQAGD
CCEEECCCCCCCCCCHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LVTAAVYEAVKKEKKPKTIKKQMKAKASDIFEDIK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 1806041