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The map label for this gene is tyrZ

Identifier: 16080897

GI number: 16080897

Start: 3947158

End: 3948399

Strand: Direct

Name: tyrZ

Synonym: BSU38460

Alternate gene names: 16080897

Gene position: 3947158-3948399 (Clockwise)

Preceding gene: 16080896

Following gene: 16080898

Centisome position: 93.63

GC content: 45.33

Gene sequence:

>1242_bases
ATGATGAGAACATTTGAGCAGCTCACAGCGTCACAACAAAAAGAGGTAGAAAGACAGCTTCAGCTATACATGACAGGCGC
CCACGAAGTCATACCGCCGGAGGAATTAAAGGCAAAGCTCGTGAAATCAATTTCCACGGGCACGCCGCTTAAAATTAAGC
TCGGATTAGATCCGTCTGCACCGGATGTACATTTGGGCCATACGGTTGTGTTAAATAAGCTTCGCCAATTTCAAGAAAAC
GGCCACATTGTCCAGCTGTTAATTGGGGATTTCACAGGAAAAATTGGTGATCCGACCGGAAAATCGGCAGCCAGAAAGCA
ACTGACTGATGAAGAAGTTCAGCACAATGCCAAAACCTACTTTGAGCAATTCGGAAAAGTGCTTGATCCAGAAAAAGTCG
AGCTTCACTATAACTCAAAATGGCTGAAAACATTGAATCTAGAAGATGTCATTGAATTAGCAGGGAAAATAACGGTAGCC
CGCCTGATGGAGCGCGACGACTTTGAAGAACGCATCGCCATGCAAAAACCAATCTCACTGCATGAATTCTTTTACCCATT
GATGCAGGGCTATGATTCTGTCGTTCTCGAAAGTGATATTGAATTAGGCGGAACGGATCAGCATTTCAATGTCCTCATGG
GACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTC
GAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACT
GCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCTTGAAGAGAAAAAACAGCTCGTAAAAGACT
TAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAA
GCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTG
GAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTAAAGCTCCTCTCTTCGAAGAGCGAGGCGCGCCGCA
TGATTCAAAACGGAGGTGTACGCATCGACGGAGAGAAAGTAACAGATGTTCACGCCAAAGCAGAGATAAGAGAGAATATG
ATCATCCAAGTCGGCAAACGCAAGTTTTTAAAGCTCCAATAA

Upstream 100 bases:

>100_bases
AGCGGAAGACAATGTCTTCAACCAGGGTGGTACCGCGTGCATTGAGCCACGTCCCTTATTGGGATGGGCTCTTTTTTGTG
TTTGTAAAAAAGGAGGAAAA

Downstream 100 bases:

>100_bases
TCTGATATTCCGAAACGCTTATGACCCTTCATTCATAAGCGTTTTTTTGCAGGTATAAAGCACTGTCTTTTCAAAAACGC
TCTCCATCTTGCAGGAAACT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 2; TyrRS 2

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MMRTFEQLTASQQKEVERQLQLYMTGAHEVIPPEELKAKLVKSISTGTPLKIKLGLDPSAPDVHLGHTVVLNKLRQFQEN
GHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVQHNAKTYFEQFGKVLDPEKVELHYNSKWLKTLNLEDVIELAGKITVA
RLMERDDFEERIAMQKPISLHEFFYPLMQGYDSVVLESDIELGGTDQHFNVLMGRHFQERYNKEKQVVILMPLLEGLDGV
EKMSKSKHNYIGINEHPNDMYGKTMSLPDSLMKKYIHLATDLELEEKKQLVKDLETGAVHPRDAKMLLARTIVRMYHGEK
AAEAAEHSFKTVFQENSLPEDIPAVNWKGEKTIAMIDLLVKLKLLSSKSEARRMIQNGGVRIDGEKVTDVHAKAEIRENM
IIQVGKRKFLKLQ

Sequences:

>Translated_413_residues
MMRTFEQLTASQQKEVERQLQLYMTGAHEVIPPEELKAKLVKSISTGTPLKIKLGLDPSAPDVHLGHTVVLNKLRQFQEN
GHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVQHNAKTYFEQFGKVLDPEKVELHYNSKWLKTLNLEDVIELAGKITVA
RLMERDDFEERIAMQKPISLHEFFYPLMQGYDSVVLESDIELGGTDQHFNVLMGRHFQERYNKEKQVVILMPLLEGLDGV
EKMSKSKHNYIGINEHPNDMYGKTMSLPDSLMKKYIHLATDLELEEKKQLVKDLETGAVHPRDAKMLLARTIVRMYHGEK
AAEAAEHSFKTVFQENSLPEDIPAVNWKGEKTIAMIDLLVKLKLLSSKSEARRMIQNGGVRIDGEKVTDVHAKAEIRENM
IIQVGKRKFLKLQ
>Mature_413_residues
MMRTFEQLTASQQKEVERQLQLYMTGAHEVIPPEELKAKLVKSISTGTPLKIKLGLDPSAPDVHLGHTVVLNKLRQFQEN
GHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVQHNAKTYFEQFGKVLDPEKVELHYNSKWLKTLNLEDVIELAGKITVA
RLMERDDFEERIAMQKPISLHEFFYPLMQGYDSVVLESDIELGGTDQHFNVLMGRHFQERYNKEKQVVILMPLLEGLDGV
EKMSKSKHNYIGINEHPNDMYGKTMSLPDSLMKKYIHLATDLELEEKKQLVKDLETGAVHPRDAKMLLARTIVRMYHGEK
AAEAAEHSFKTVFQENSLPEDIPAVNWKGEKTIAMIDLLVKLKLLSSKSEARRMIQNGGVRIDGEKVTDVHAKAEIRENM
IIQVGKRKFLKLQ

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=422, Percent_Identity=24.8815165876777, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1787925, Length=365, Percent_Identity=28.2191780821918, Blast_Score=141, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI25145802, Length=379, Percent_Identity=24.5382585751979, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=421, Percent_Identity=26.3657957244656, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130383, Length=425, Percent_Identity=26.8235294117647, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY2_BACSU (P25151)

Other databases:

- EMBL:   X52480
- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S16426
- RefSeq:   NP_391725.1
- ProteinModelPortal:   P25151
- SMR:   P25151
- EnsemblBacteria:   EBBACT00000001475
- GeneID:   937345
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38460
- NMPDR:   fig|224308.1.peg.3851
- GenoList:   BSU38460
- GeneTree:   EBGT00050000000599
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK13354
- BioCyc:   BSUB:BSU38460-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47101; Mature: 47101

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 245-245

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTFEQLTASQQKEVERQLQLYMTGAHEVIPPEELKAKLVKSISTGTPLKIKLGLDPSA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
PDVHLGHTVVLNKLRQFQENGHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVQHNAKTY
CCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHH
FEQFGKVLDPEKVELHYNSKWLKTLNLEDVIELAGKITVARLMERDDFEERIAMQKPISL
HHHHHHCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHH
HEFFYPLMQGYDSVVLESDIELGGTDQHFNVLMGRHFQERYNKEKQVVILMPLLEGLDGV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCHHH
EKMSKSKHNYIGINEHPNDMYGKTMSLPDSLMKKYIHLATDLELEEKKQLVKDLETGAVH
HHHHHCCCCEECCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PRDAKMLLARTIVRMYHGEKAAEAAEHSFKTVFQENSLPEDIPAVNWKGEKTIAMIDLLV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KLKLLSSKSEARRMIQNGGVRIDGEKVTDVHAKAEIRENMIIQVGKRKFLKLQ
HHHHHHCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECC
>Mature Secondary Structure
MMRTFEQLTASQQKEVERQLQLYMTGAHEVIPPEELKAKLVKSISTGTPLKIKLGLDPSA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
PDVHLGHTVVLNKLRQFQENGHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVQHNAKTY
CCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHH
FEQFGKVLDPEKVELHYNSKWLKTLNLEDVIELAGKITVARLMERDDFEERIAMQKPISL
HHHHHHCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHH
HEFFYPLMQGYDSVVLESDIELGGTDQHFNVLMGRHFQERYNKEKQVVILMPLLEGLDGV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCHHH
EKMSKSKHNYIGINEHPNDMYGKTMSLPDSLMKKYIHLATDLELEEKKQLVKDLETGAVH
HHHHHCCCCEECCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PRDAKMLLARTIVRMYHGEKAAEAAEHSFKTVFQENSLPEDIPAVNWKGEKTIAMIDLLV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KLKLLSSKSEARRMIQNGGVRIDGEKVTDVHAKAEIRENMIIQVGKRKFLKLQ
HHHHHHCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1806041; 7934828; 9384377