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The map label for this gene is spsI

Identifier: 16080835

GI number: 16080835

Start: 3885239

End: 3885979

Strand: Reverse

Name: spsI

Synonym: BSU37840

Alternate gene names: 16080835

Gene position: 3885979-3885239 (Counterclockwise)

Preceding gene: 16080836

Following gene: 16080834

Centisome position: 92.18

GC content: 44.4

Gene sequence:

>741_bases
GTGAAAGGTGTTATTTTAGCCGGAGGAAACGGCTCACGCCTTATGCCCTTAACGAAAGCCGTCAACAAGCATTTGCTTCC
TGTAGGCCCGTATCCGATGATATACTGGTCGATTATGAAGCTGCAGGAAGCCGGAATAAAAGACATTTTACTCATCAGCC
AAAAGGAGCATATGCCGCAATTTTACAAACTGCTTGGGAACGGGGAAGAGCTTGGCGTTACTATCACTTATCAAGTACAG
CCGGCAGCATCGGGCATTTCGGATGGGCTGTCTTATGCAAAGCGCTTTACGAAAAAAGAGTCTTTCATTTTATTATTAGG
TGATAATATATTTGAAGATTCATTAAAGCCTTATACAGAACGCTTTGAACAGCAGGGGAAAGGAGCAAAAGTGCTTCTGA
AAGAAGTTGATGACCCGGAACGTTTCGGGATTGCTGAGATTGATGAGAAAAACAAGCGGATTCGTTCCATCATCGAAAAG
CCCGAACAGCCACCGACCAACCTTTGTGTTACCGGCATTTATATGTATGATGCAGAGGTGTTTTCATATATTGAACAAAT
ATCACCATCAAAGCGCGGCGAGCTGGAAATCACCGATGTGAATAATCTATACATCGAAAACAGCCAGCTTACGTATGATG
TGTTATCAGGCTGGTGGGTAGATGCCGGAACTCACGAATCGCTTTATCTCGCTTCCCAGCTTGTACACCAGGCTTTACGG
AAAGGACAGGACGAAAAGTGA

Upstream 100 bases:

>100_bases
AAGGCCGCTCGTGGATGGCAAAGGCATTATACGGGTAACAGCTATTCTTCAAGATTTATATGAACAAGAGATATAAAAAT
GCATGTAAGGAAGTGTTAAT

Downstream 100 bases:

>100_bases
TGGCAAAATCATATTTAATTACAGGCGGGGCCGGCTTCATCGGGCTCACGTTTACAAAGCTCATGCTGAGGGAAACGGAT
GCGCGCATTACCGTTTTAGA

Product: dTDP-glucose pyrophosphorylase

Products: diphosphate; dTDPglucose

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKGVILAGGNGSRLMPLTKAVNKHLLPVGPYPMIYWSIMKLQEAGIKDILLISQKEHMPQFYKLLGNGEELGVTITYQVQ
PAASGISDGLSYAKRFTKKESFILLLGDNIFEDSLKPYTERFEQQGKGAKVLLKEVDDPERFGIAEIDEKNKRIRSIIEK
PEQPPTNLCVTGIYMYDAEVFSYIEQISPSKRGELEITDVNNLYIENSQLTYDVLSGWWVDAGTHESLYLASQLVHQALR
KGQDEK

Sequences:

>Translated_246_residues
MKGVILAGGNGSRLMPLTKAVNKHLLPVGPYPMIYWSIMKLQEAGIKDILLISQKEHMPQFYKLLGNGEELGVTITYQVQ
PAASGISDGLSYAKRFTKKESFILLLGDNIFEDSLKPYTERFEQQGKGAKVLLKEVDDPERFGIAEIDEKNKRIRSIIEK
PEQPPTNLCVTGIYMYDAEVFSYIEQISPSKRGELEITDVNNLYIENSQLTYDVLSGWWVDAGTHESLYLASQLVHQALR
KGQDEK
>Mature_246_residues
MKGVILAGGNGSRLMPLTKAVNKHLLPVGPYPMIYWSIMKLQEAGIKDILLISQKEHMPQFYKLLGNGEELGVTITYQVQ
PAASGISDGLSYAKRFTKKESFILLLGDNIFEDSLKPYTERFEQQGKGAKVLLKEVDDPERFGIAEIDEKNKRIRSIIEK
PEQPPTNLCVTGIYMYDAEVFSYIEQISPSKRGELEITDVNNLYIENSQLTYDVLSGWWVDAGTHESLYLASQLVHQALR
KGQDEK

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family

Homologues:

Organism=Homo sapiens, GI11761621, Length=250, Percent_Identity=24.8, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI11761619, Length=250, Percent_Identity=25.2, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1790224, Length=249, Percent_Identity=44.578313253012, Blast_Score=203, Evalue=9e-54,
Organism=Escherichia coli, GI1788351, Length=237, Percent_Identity=43.4599156118143, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1788355, Length=261, Percent_Identity=24.5210727969349, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787488, Length=261, Percent_Identity=23.7547892720307, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133931050, Length=228, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320148, Length=249, Percent_Identity=30.9236947791165, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=27.4900398406374, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=27.4900398406374, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPSI_BACSU (P39629)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39726
- RefSeq:   NP_391663.1
- ProteinModelPortal:   P39629
- SMR:   P39629
- EnsemblBacteria:   EBBACT00000002702
- GeneID:   937229
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37840
- NMPDR:   fig|224308.1.peg.3789
- GenoList:   BSU37840
- GeneTree:   EBGT00050000000561
- HOGENOM:   HBG688195
- OMA:   DAEVFSY
- PhylomeDB:   P39629
- ProtClustDB:   CLSK888079
- BioCyc:   BSUB:BSU37840-MONOMER
- InterPro:   IPR005835

Pfam domain/function: PF00483 NTP_transferase

EC number: 2.7.7.24

Molecular weight: Translated: 27773; Mature: 27773

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVILAGGNGSRLMPLTKAVNKHLLPVGPYPMIYWSIMKLQEAGIKDILLISQKEHMPQ
CCCEEEECCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
FYKLLGNGEELGVTITYQVQPAASGISDGLSYAKRFTKKESFILLLGDNIFEDSLKPYTE
HHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHCHHHHH
RFEQQGKGAKVLLKEVDDPERFGIAEIDEKNKRIRSIIEKPEQPPTNLCVTGIYMYDAEV
HHHHCCCCCEEHEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHH
FSYIEQISPSKRGELEITDVNNLYIENSQLTYDVLSGWWVDAGTHESLYLASQLVHQALR
HHHHHHHCCCCCCCEEEEECCEEEEECCCEEEEHHCCEEECCCCCCHHHHHHHHHHHHHH
KGQDEK
CCCCCC
>Mature Secondary Structure
MKGVILAGGNGSRLMPLTKAVNKHLLPVGPYPMIYWSIMKLQEAGIKDILLISQKEHMPQ
CCCEEEECCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
FYKLLGNGEELGVTITYQVQPAASGISDGLSYAKRFTKKESFILLLGDNIFEDSLKPYTE
HHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHCHHHHH
RFEQQGKGAKVLLKEVDDPERFGIAEIDEKNKRIRSIIEKPEQPPTNLCVTGIYMYDAEV
HHHHCCCCCEEHEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHH
FSYIEQISPSKRGELEITDVNNLYIENSQLTYDVLSGWWVDAGTHESLYLASQLVHQALR
HHHHHHHCCCCCCCEEEEECCEEEEECCCEEEEHHCCEEECCCCCCHHHHHHHHHHHHHH
KGQDEK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377