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The map label for this gene is spsJ

Identifier: 16080834

GI number: 16080834

Start: 3884292

End: 3885239

Strand: Reverse

Name: spsJ

Synonym: BSU37830

Alternate gene names: 16080834

Gene position: 3885239-3884292 (Counterclockwise)

Preceding gene: 16080835

Following gene: 255767795

Centisome position: 92.16

GC content: 49.68

Gene sequence:

>948_bases
ATGGCAAAATCATATTTAATTACAGGCGGGGCCGGCTTCATCGGGCTCACGTTTACAAAGCTCATGCTGAGGGAAACGGA
TGCGCGCATTACCGTTTTAGATAAACTGACGTACGCCAGCCACCCGGAGGAAATGGAAAAATTAAAGCAAAACAGCCGTT
TTCGCTTTGTAAAAGGGGACATCAGCGTTCAGGAGGATATCGACCGCGCTTTTGACGAGACCTACGATGGCGTCATTCAC
TTCGCAGCAGAATCGCATGTGGACAGAAGCATTTCCCAAGCGGAGCCGTTTATCACCACAAATGTCATGGGGACATACCG
ATTGGCGGAAGCGGTCTTGAAAGGAAAAGCCAAGAAGCTGATTCATATTTCAACAGATGAGGTATACGGGGATTTAAAAG
CGGATGATCCCGCGTTTACAGAAACGACGCCGCTTTCTCCTAATAACCCATACTCAGCCAGCAAAGCAAGCTCTGATTTG
CTCGTGTTGTCTTATGTGAAAACCCACAAGCTTCCGGCCATCATTACACGATGCAGCAACAATTACGGACCTTATCAGCA
CAGCGAAAAAATGATTCCGACGATTATCCGTCATGCGAAGCAGGGCCTGCCTGTTCCGCTGTATGGCGATGGACTCCAGA
TCCGAGACTGGCTGTTTGCGGAGGATCATTGCCGCGCGATCAAGCTGATTTTAGAAAAAGGGACCGACGGCGAGGTGTAT
AACATCGGCGGCGGAAATGAACGCACGAATAAAGAGCTGGCGAGTGTCATTTTGAAGCATCTGGGCTGTGAGGAATTGTT
CGCCCACGTGGAAGACCGAAAAGGGCATGACCGGCGCTATGCGATCAATGCATCAAAGCTGAAAAACGAACTGGGCTGGC
GGCAGGAAGTGACGTTTGAAGAAGGCATTGCGCGTACGATTCAGTGGTATACAGACAATGACCGATGA

Upstream 100 bases:

>100_bases
TGTTATCAGGCTGGTGGGTAGATGCCGGAACTCACGAATCGCTTTATCTCGCTTCCCAGCTTGTACACCAGGCTTTACGG
AAAGGACAGGACGAAAAGTG

Downstream 100 bases:

>100_bases
GAAGGTGATCTGATTGACAAAGGTATTGGTGACTGGGGCGGGAGGACAGCTTGGCTTGGAACTATGCCGCCAGCTGAAGC
AAGCCGGATATGAGGTCATT

Product: dTDP-glucose 4,6-dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MAKSYLITGGAGFIGLTFTKLMLRETDARITVLDKLTYASHPEEMEKLKQNSRFRFVKGDISVQEDIDRAFDETYDGVIH
FAAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKLIHISTDEVYGDLKADDPAFTETTPLSPNNPYSASKASSDL
LVLSYVKTHKLPAIITRCSNNYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVY
NIGGGNERTNKELASVILKHLGCEELFAHVEDRKGHDRRYAINASKLKNELGWRQEVTFEEGIARTIQWYTDNDR

Sequences:

>Translated_315_residues
MAKSYLITGGAGFIGLTFTKLMLRETDARITVLDKLTYASHPEEMEKLKQNSRFRFVKGDISVQEDIDRAFDETYDGVIH
FAAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKLIHISTDEVYGDLKADDPAFTETTPLSPNNPYSASKASSDL
LVLSYVKTHKLPAIITRCSNNYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVY
NIGGGNERTNKELASVILKHLGCEELFAHVEDRKGHDRRYAINASKLKNELGWRQEVTFEEGIARTIQWYTDNDR
>Mature_314_residues
AKSYLITGGAGFIGLTFTKLMLRETDARITVLDKLTYASHPEEMEKLKQNSRFRFVKGDISVQEDIDRAFDETYDGVIHF
AAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKLIHISTDEVYGDLKADDPAFTETTPLSPNNPYSASKASSDLL
VLSYVKTHKLPAIITRCSNNYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVYN
IGGGNERTNKELASVILKHLGCEELFAHVEDRKGHDRRYAINASKLKNELGWRQEVTFEEGIARTIQWYTDNDR

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family

Homologues:

Organism=Homo sapiens, GI7657641, Length=322, Percent_Identity=38.1987577639752, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI42516563, Length=314, Percent_Identity=24.8407643312102, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI48994969, Length=331, Percent_Identity=48.6404833836858, Blast_Score=306, Evalue=9e-85,
Organism=Escherichia coli, GI1788353, Length=336, Percent_Identity=45.2380952380952, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1786974, Length=331, Percent_Identity=27.4924471299094, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788365, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=321, Percent_Identity=39.5638629283489, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI115532424, Length=321, Percent_Identity=34.2679127725857, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=315, Percent_Identity=24.4444444444444, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=341, Percent_Identity=24.633431085044, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=343, Percent_Identity=24.198250728863, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=345, Percent_Identity=25.5072463768116, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356223, Length=316, Percent_Identity=26.2658227848101, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19923002, Length=343, Percent_Identity=25.3644314868805, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPSJ_BACSU (P39630)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39727
- RefSeq:   NP_391662.1
- ProteinModelPortal:   P39630
- SMR:   P39630
- EnsemblBacteria:   EBBACT00000001995
- GeneID:   937227
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37830
- NMPDR:   fig|224308.1.peg.3788
- GenoList:   BSU37830
- GeneTree:   EBGT00070000031836
- HOGENOM:   HBG755066
- OMA:   SGSYRDW
- PhylomeDB:   P39630
- ProtClustDB:   CLSK888078
- BioCyc:   BSUB:BSU37830-MONOMER
- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01181

Pfam domain/function: PF01370 Epimerase

EC number: 4.2.1.46

Molecular weight: Translated: 35540; Mature: 35408

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: ACT_SITE 151-151 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSYLITGGAGFIGLTFTKLMLRETDARITVLDKLTYASHPEEMEKLKQNSRFRFVKGD
CCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHCCCEEEEECC
ISVQEDIDRAFDETYDGVIHFAAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKL
CCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEE
IHISTDEVYGDLKADDPAFTETTPLSPNNPYSASKASSDLLVLSYVKTHKLPAIITRCSN
EEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCHHHHHHHHCC
NYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVY
CCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCEEHHHHCHHHHHHHHHHHHHCCCCCCEE
NIGGGNERTNKELASVILKHLGCEELFAHVEDRKGHDRRYAINASKLKNELGWRQEVTFE
ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCCCCHH
EGIARTIQWYTDNDR
HHHHHEEEEEECCCC
>Mature Secondary Structure 
AKSYLITGGAGFIGLTFTKLMLRETDARITVLDKLTYASHPEEMEKLKQNSRFRFVKGD
CCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHCCCEEEEECC
ISVQEDIDRAFDETYDGVIHFAAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKL
CCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEE
IHISTDEVYGDLKADDPAFTETTPLSPNNPYSASKASSDLLVLSYVKTHKLPAIITRCSN
EEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCHHHHHHHHCC
NYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVY
CCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCEEHHHHCHHHHHHHHHHHHHCCCCCCEE
NIGGGNERTNKELASVILKHLGCEELFAHVEDRKGHDRRYAINASKLKNELGWRQEVTFE
ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCCCCHH
EGIARTIQWYTDNDR
HHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377