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The map label for this gene is mmr

Identifier: 16080809

GI number: 16080809

Start: 3857017

End: 3858417

Strand: Reverse

Name: mmr

Synonym: BSU37570

Alternate gene names: 16080809

Gene position: 3858417-3857017 (Counterclockwise)

Preceding gene: 16080810

Following gene: 255767793

Centisome position: 91.53

GC content: 51.82

Gene sequence:

>1401_bases
ATGAAAAATAGCGGGTCTATACAAGAATCAACATCATCAACAGGCATATCCGTGCTGATCGTGCTCGCACTTGGTTTCCT
CATGGCAACTCTTGATGTGACCGTTGTGAATGTGGCGATGGCTGATATGAAAAACACTCTTTCAATGTCACTTTCAGGTG
TAACGTGGGTTGTGGACGGGTACATTCTTACTTTTGCGTCGCTTCTGCTGGCGGGCGGAGCCTTGGCTGACCGTTTCGGC
TCGAAGACGATCTATATCTTGGGTCTAGCCGTTTTTGTTATGGCCTCCTGTCTATGCGCTGCATCTATAAATGGGCAGAT
GCTGATTGCCGGACGTCTCATACAGGGAATTGGGGCAGCTTTGTTTATGCCAAGCTCACTCAGCCTTTTGGCTGCCTCTT
ACCTTGATGAGCGGGCGCGGGCGAGAATGTTTGGACTGTGGGCCGCGCTTGTTTCCGCCGCTTCAGCCCTTGGGCCTTTT
ATCGGGGGCGTGCTGGTACAGTTGGCGGGCTGGCAGAGTATTTTTCTGATCAATGTGCCAATAGGGGCTGCTGCGCTCAT
TTCAGCTTACCGAATCCTCAGCCGCGTTCCTGGGAAAAGCAGCCGCGTGAACATCATCGGGCATCTGCTTGGCATGATGG
CTCTCGGGTTTCTTTCCTATGCTTTGATACAGGGGCCTTCAGCGGGCTGGCGCTCGCCAGTGATTCTTGTAGCATTTACT
GCAGCGGTTTTGGCATTTGTGCTGTTTTTGCTGCGGGAAATTTCTGCAAAAACACCCATCCTGCCGGCGTCCTTATACAA
AAACGGCCGCTTCTCAGCTGCCCAATTTATCGGGTTTTTGCTGAATTTCGCCCTTTTTGGCGGCATGTTTATGCTCAGCC
TTTTTTTGCAGGAAGCAGGAGGGGCAAGTTCCTTTATGGCTGGAGTGGAGCTGCTGCCGATGATGGCGGTCTTCGTGATC
GGCAATCTGCTGTTTGCCCGTCTGGCGAATCGGTTTGAAGCCGGTCAGCTTATGTTTGTATCAATGGCGGTCAGCTGTAT
CATTGCATTGCTGTTGTTTGTGCTGATTTCTCCGGATTTTCCTTATTGGCAGCTGGCGGTGTTGATGTCTGTTATGAACC
TCTGTACCGGTATTACAGTTCCTGCCATGACAACTGTGATCATGCAGGCTGCCGGGCAAAGACATACGAATATCGCCGGT
GCGGCGTTAAATGCAAACCGGCAAATCGGAGCCTTGGTCGGCGTAGCGATAACCGGGGTGATCATTCATCTGTCCGCCAC
TTGGTATGCTGGTGCCGGGTTTGCGTTTTTGATGATGGGAGCGGCATACAGCCTCGCTGCATTGCTTGTATGGCTGTTTC
TGGCTGCCCATAACGGGACTGCCGCGTCAGAAAAAATGTAA

Upstream 100 bases:

>100_bases
GAGAAGAATCCTTGCAGCATGTTATGGACAGAGTCGGGGAATTGTATGATCTTGAAAAAATAGATGATTGGGTAATCAAG
CAAGTGAAAGGGCAATTGTA

Downstream 100 bases:

>100_bases
ATCACTGATTGCGCTTTGTGGGAATATATAGTAAGATATAAAAAATTATTGAAACAACAGGCACTGATAAGGACACGAGA
TGCTTTTTACGGTTTCCAGA

Product: toxic compound efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: MMR peptide

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKNSGSIQESTSSTGISVLIVLALGFLMATLDVTVVNVAMADMKNTLSMSLSGVTWVVDGYILTFASLLLAGGALADRFG
SKTIYILGLAVFVMASCLCAASINGQMLIAGRLIQGIGAALFMPSSLSLLAASYLDERARARMFGLWAALVSAASALGPF
IGGVLVQLAGWQSIFLINVPIGAAALISAYRILSRVPGKSSRVNIIGHLLGMMALGFLSYALIQGPSAGWRSPVILVAFT
AAVLAFVLFLLREISAKTPILPASLYKNGRFSAAQFIGFLLNFALFGGMFMLSLFLQEAGGASSFMAGVELLPMMAVFVI
GNLLFARLANRFEAGQLMFVSMAVSCIIALLLFVLISPDFPYWQLAVLMSVMNLCTGITVPAMTTVIMQAAGQRHTNIAG
AALNANRQIGALVGVAITGVIIHLSATWYAGAGFAFLMMGAAYSLAALLVWLFLAAHNGTAASEKM

Sequences:

>Translated_466_residues
MKNSGSIQESTSSTGISVLIVLALGFLMATLDVTVVNVAMADMKNTLSMSLSGVTWVVDGYILTFASLLLAGGALADRFG
SKTIYILGLAVFVMASCLCAASINGQMLIAGRLIQGIGAALFMPSSLSLLAASYLDERARARMFGLWAALVSAASALGPF
IGGVLVQLAGWQSIFLINVPIGAAALISAYRILSRVPGKSSRVNIIGHLLGMMALGFLSYALIQGPSAGWRSPVILVAFT
AAVLAFVLFLLREISAKTPILPASLYKNGRFSAAQFIGFLLNFALFGGMFMLSLFLQEAGGASSFMAGVELLPMMAVFVI
GNLLFARLANRFEAGQLMFVSMAVSCIIALLLFVLISPDFPYWQLAVLMSVMNLCTGITVPAMTTVIMQAAGQRHTNIAG
AALNANRQIGALVGVAITGVIIHLSATWYAGAGFAFLMMGAAYSLAALLVWLFLAAHNGTAASEKM
>Mature_466_residues
MKNSGSIQESTSSTGISVLIVLALGFLMATLDVTVVNVAMADMKNTLSMSLSGVTWVVDGYILTFASLLLAGGALADRFG
SKTIYILGLAVFVMASCLCAASINGQMLIAGRLIQGIGAALFMPSSLSLLAASYLDERARARMFGLWAALVSAASALGPF
IGGVLVQLAGWQSIFLINVPIGAAALISAYRILSRVPGKSSRVNIIGHLLGMMALGFLSYALIQGPSAGWRSPVILVAFT
AAVLAFVLFLLREISAKTPILPASLYKNGRFSAAQFIGFLLNFALFGGMFMLSLFLQEAGGASSFMAGVELLPMMAVFVI
GNLLFARLANRFEAGQLMFVSMAVSCIIALLLFVLISPDFPYWQLAVLMSVMNLCTGITVPAMTTVIMQAAGQRHTNIAG
AALNANRQIGALVGVAITGVIIHLSATWYAGAGFAFLMMGAAYSLAALLVWLFLAAHNGTAASEKM

Specific function: Resistance to the epoxide antibiotic methylenomycin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=28.6407766990291, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789042, Length=325, Percent_Identity=30.7692307692308, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081983, Length=321, Percent_Identity=30.8411214953271, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788710, Length=368, Percent_Identity=27.445652173913, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788392, Length=416, Percent_Identity=27.1634615384615, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790146, Length=146, Percent_Identity=30.1369863013699, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=384, Percent_Identity=22.9166666666667, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319770, Length=398, Percent_Identity=25.6281407035176, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=234, Percent_Identity=26.4957264957265, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=156, Percent_Identity=32.0512820512821, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323520, Length=227, Percent_Identity=25.5506607929515, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MMR_BACSU (Q00538)

Other databases:

- EMBL:   X66121
- EMBL:   AL009126
- PIR:   I40493
- RefSeq:   NP_391637.1
- ProteinModelPortal:   Q00538
- EnsemblBacteria:   EBBACT00000001061
- GeneID:   937101
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37570
- NMPDR:   fig|224308.1.peg.3763
- GenoList:   BSU37570
- GeneTree:   EBGT00070000031968
- HOGENOM:   HBG679341
- PhylomeDB:   Q00538
- BioCyc:   BSUB:BSU37570-MONOMER
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 48846; Mature: 48846

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13126550)-; HASH(0x1308b35c)-; HASH(0x13181a4c)-; HASH(0x12e38c00)-; HASH(0x1317b9e4)-; HASH(0x11d85cec)-; HASH(0x13184810)-; HASH(0x1314f298)-; HASH(0x131848c4)-; HASH(0x13184774)-; HASH(0x13142bb8)-; HASH(0x131400e0)-; HASH(0x13142b7c)-; HASH(0x13187200)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSGSIQESTSSTGISVLIVLALGFLMATLDVTVVNVAMADMKNTLSMSLSGVTWVVDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YILTFASLLLAGGALADRFGSKTIYILGLAVFVMASCLCAASINGQMLIAGRLIQGIGAA
HHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHH
LFMPSSLSLLAASYLDERARARMFGLWAALVSAASALGPFIGGVLVQLAGWQSIFLINVP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
IGAAALISAYRILSRVPGKSSRVNIIGHLLGMMALGFLSYALIQGPSAGWRSPVILVAFT
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AAVLAFVLFLLREISAKTPILPASLYKNGRFSAAQFIGFLLNFALFGGMFMLSLFLQEAG
HHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GASSFMAGVELLPMMAVFVIGNLLFARLANRFEAGQLMFVSMAVSCIIALLLFVLISPDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PYWQLAVLMSVMNLCTGITVPAMTTVIMQAAGQRHTNIAGAALNANRQIGALVGVAITGV
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHEECCCCHHHHHHHHHHHHH
IIHLSATWYAGAGFAFLMMGAAYSLAALLVWLFLAAHNGTAASEKM
HHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure
MKNSGSIQESTSSTGISVLIVLALGFLMATLDVTVVNVAMADMKNTLSMSLSGVTWVVDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YILTFASLLLAGGALADRFGSKTIYILGLAVFVMASCLCAASINGQMLIAGRLIQGIGAA
HHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHH
LFMPSSLSLLAASYLDERARARMFGLWAALVSAASALGPFIGGVLVQLAGWQSIFLINVP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
IGAAALISAYRILSRVPGKSSRVNIIGHLLGMMALGFLSYALIQGPSAGWRSPVILVAFT
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AAVLAFVLFLLREISAKTPILPASLYKNGRFSAAQFIGFLLNFALFGGMFMLSLFLQEAG
HHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GASSFMAGVELLPMMAVFVIGNLLFARLANRFEAGQLMFVSMAVSCIIALLLFVLISPDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PYWQLAVLMSVMNLCTGITVPAMTTVIMQAAGQRHTNIAGAALNANRQIGALVGVAITGV
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHEECCCCHHHHHHHHHHHHH
IIHLSATWYAGAGFAFLMMGAAYSLAALLVWLFLAAHNGTAASEKM
HHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1379177; 9384377