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The map label for this gene is thrZ

Identifier: 255767793

GI number: 255767793

Start: 3854256

End: 3856172

Strand: Reverse

Name: thrZ

Synonym: BSU37560

Alternate gene names: 255767793

Gene position: 3856172-3854256 (Counterclockwise)

Preceding gene: 16080809

Following gene: 16080807

Centisome position: 91.47

GC content: 48.93

Gene sequence:

>1917_bases
ATGAGCAAACATGTACACATTCAGCTTCCGGACGGACAGATCCAGGAATATCCGAAAGGCATCACAATCAAAGAAGCGGC
TGGTTCGATCAGTTCCAGTCTGCAAAAGAAGGCGGCCGCAGGTCAGGTCAACGGCAAGCTTGTTGATCTCAGTTTCAAGC
TGGAGGAGGATGCTGAGCTTTCGATTGTCACACTCGATTCACAAGAAGGCTTGCAGGTGCTTCGCCACACAACAGCGCAC
GTGCTGGCCCAAGCAGTAAAACGGCTGTACGGCGAAGTGTCATTGGGGGTAGGGCCTGTGATTTTAGACGGCTTCTACTA
TGACATGAAGCTGGGGAAAAGCTTGGCATCAGGAGACTTAGAGGCCATCGAAAAAGAAATGAAAAACATAATAAATGAGA
ACCTTGAAATCAAACGTATAGAGGTTTCTTACGAAGAAGCTGAAGAGCTGTTTGCACAAAAAGACGAGCGGCTCAAGCTT
GAGATTTTAAAGGATATTCCGCGCGGGGAGGATATCACACTGTACCAGCAAGGGGAATTCGTTGACCTGTGCCGAGGGCC
GCATCTTCCTTCTACAGGGATGATCAAGGCGTTCAAACTGACAAGAGTATCAGGCGCTTATTGGCGCGGAGACAGCAAAA
ATGAAGTCCTGCAGCGGGTGTACGGGGTTGCGTTTCAAAAGAAAAAAGATTTGGATGCGCACCTGCATATGCTAGAAGAA
GCGGCAAAACGAGACCATCGCAAGCTCGGCAAACAGTTAGGGCTGTTTATGTTTTCTGAAGAAGCGCCGGGGATGCCGTT
TTATTTGCCGAAAGGGCAGATTGTCCGCAATGAGCTGGAGCGCTTCTCGCGTGAGCTGCAAACAAATGCCGGCTACGATG
AGGTGCGTACGCCGTTTATGATGAATCAGCGGTTATGGGAGCAGTCAGGCCATTGGGACCATTATCGCGACAATATGTAC
TTTTCAGAAGTGGATGATACAAGATTCGCAATGAAGCCGATGAATTGCCCCGGCCACATGCTCATCTTTAAAAACAGCCT
GTATTCTTATCGGGATCTGCCGATTCGCATGGCGGAATTCGGACAGGTGCACCGCCATGAATACAGCGGCGCTTTAAACG
GAATGCTTCGCGTTCGGACGTTCTGTCAGGATGACGCCCATATTTTCGTTCGGGAGGATCAGATTGAGAGTGAGATCAAA
GAAGCGATTCGGCTGATTGATGAGGTATATCGGACATTTGGTTTCGAGTATTCTGTTGAGCTTTCGACTCGCCCGGAGGA
TTCATTAGGCGATGACAGCCTTTGGGAAGCGTCGGAACGTGCCTTAGCACGCGTCCTTGAGGAGCTTGGCCTCTCTTATG
AGATCAATGAAGGCGATGGGGCGTTTTACGGGCCGAAAATTGATTTCCATATAAAAGATGCCCTGAAACGGAGCCACCAA
TGCGCGACAATTCAGCTTGATTTTCAAATGCCGGAAAAATTTGATTTAACATATATCAACGAACTCAATGAGAAGGTTCG
TCCCGTTGTCATCCACAGAGCGGTTTTCGGCTCAATCGACCGTTTCTTCGGCATTTTGATCGAGCATTACGGTGGGGCTT
TTCCAGTCTGGCTCGCACCGATCCAGGTTCAAATCATACCGGTTTCGCACGTTCACTTAGACTATTGCAGAAAAGTGCAG
GCGGAGCTGAAGCAGGCGGGGATAAGAGCCGGCATTGATGAGCGCAATGAGAAGCTCGGCTATAAAATCAGAGAATCGCA
GGTGCAAAAAATTCCGTACGTGCTGGTGCTTGGAGATCATGAGGAGCAGGAGAATGCGGTAAACGTCCGCCGATTCGGAC
ATCAGCAGAATGAACACGTTCCTTTCCAAACTTTTAAAGATAAACTTGTGAAACAGGTGGAAAATCGAGGCATGTAG

Upstream 100 bases:

>100_bases
CGGGAACTTGGGTGGAACCACGGGTTAATCACACACTCGTCCCTATCTGCGGGACGGGTGTGTTTTTTTATATACAACAA
TGAATAAGGAGTGTTCTAAA

Downstream 100 bases:

>100_bases
GATAAAAGCTGTGAATTCACATTTTGCTTTTTTGACTTTCCCTATTCGGAATCAGTAAAATAATAAGTAAATCATATCGT
TCGTATGTTATGAATGCATC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSKHVHIQLPDGQIQEYPKGITIKEAAGSISSSLQKKAAAGQVNGKLVDLSFKLEEDAELSIVTLDSQEGLQVLRHTTAH
VLAQAVKRLYGEVSLGVGPVILDGFYYDMKLGKSLASGDLEAIEKEMKNIINENLEIKRIEVSYEEAEELFAQKDERLKL
EILKDIPRGEDITLYQQGEFVDLCRGPHLPSTGMIKAFKLTRVSGAYWRGDSKNEVLQRVYGVAFQKKKDLDAHLHMLEE
AAKRDHRKLGKQLGLFMFSEEAPGMPFYLPKGQIVRNELERFSRELQTNAGYDEVRTPFMMNQRLWEQSGHWDHYRDNMY
FSEVDDTRFAMKPMNCPGHMLIFKNSLYSYRDLPIRMAEFGQVHRHEYSGALNGMLRVRTFCQDDAHIFVREDQIESEIK
EAIRLIDEVYRTFGFEYSVELSTRPEDSLGDDSLWEASERALARVLEELGLSYEINEGDGAFYGPKIDFHIKDALKRSHQ
CATIQLDFQMPEKFDLTYINELNEKVRPVVIHRAVFGSIDRFFGILIEHYGGAFPVWLAPIQVQIIPVSHVHLDYCRKVQ
AELKQAGIRAGIDERNEKLGYKIRESQVQKIPYVLVLGDHEEQENAVNVRRFGHQQNEHVPFQTFKDKLVKQVENRGM

Sequences:

>Translated_638_residues
MSKHVHIQLPDGQIQEYPKGITIKEAAGSISSSLQKKAAAGQVNGKLVDLSFKLEEDAELSIVTLDSQEGLQVLRHTTAH
VLAQAVKRLYGEVSLGVGPVILDGFYYDMKLGKSLASGDLEAIEKEMKNIINENLEIKRIEVSYEEAEELFAQKDERLKL
EILKDIPRGEDITLYQQGEFVDLCRGPHLPSTGMIKAFKLTRVSGAYWRGDSKNEVLQRVYGVAFQKKKDLDAHLHMLEE
AAKRDHRKLGKQLGLFMFSEEAPGMPFYLPKGQIVRNELERFSRELQTNAGYDEVRTPFMMNQRLWEQSGHWDHYRDNMY
FSEVDDTRFAMKPMNCPGHMLIFKNSLYSYRDLPIRMAEFGQVHRHEYSGALNGMLRVRTFCQDDAHIFVREDQIESEIK
EAIRLIDEVYRTFGFEYSVELSTRPEDSLGDDSLWEASERALARVLEELGLSYEINEGDGAFYGPKIDFHIKDALKRSHQ
CATIQLDFQMPEKFDLTYINELNEKVRPVVIHRAVFGSIDRFFGILIEHYGGAFPVWLAPIQVQIIPVSHVHLDYCRKVQ
AELKQAGIRAGIDERNEKLGYKIRESQVQKIPYVLVLGDHEEQENAVNVRRFGHQQNEHVPFQTFKDKLVKQVENRGM
>Mature_637_residues
SKHVHIQLPDGQIQEYPKGITIKEAAGSISSSLQKKAAAGQVNGKLVDLSFKLEEDAELSIVTLDSQEGLQVLRHTTAHV
LAQAVKRLYGEVSLGVGPVILDGFYYDMKLGKSLASGDLEAIEKEMKNIINENLEIKRIEVSYEEAEELFAQKDERLKLE
ILKDIPRGEDITLYQQGEFVDLCRGPHLPSTGMIKAFKLTRVSGAYWRGDSKNEVLQRVYGVAFQKKKDLDAHLHMLEEA
AKRDHRKLGKQLGLFMFSEEAPGMPFYLPKGQIVRNELERFSRELQTNAGYDEVRTPFMMNQRLWEQSGHWDHYRDNMYF
SEVDDTRFAMKPMNCPGHMLIFKNSLYSYRDLPIRMAEFGQVHRHEYSGALNGMLRVRTFCQDDAHIFVREDQIESEIKE
AIRLIDEVYRTFGFEYSVELSTRPEDSLGDDSLWEASERALARVLEELGLSYEINEGDGAFYGPKIDFHIKDALKRSHQC
ATIQLDFQMPEKFDLTYINELNEKVRPVVIHRAVFGSIDRFFGILIEHYGGAFPVWLAPIQVQIIPVSHVHLDYCRKVQA
ELKQAGIRAGIDERNEKLGYKIRESQVQKIPYVLVLGDHEEQENAVNVRRFGHQQNEHVPFQTFKDKLVKQVENRGM

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=641, Percent_Identity=42.7457098283931, Blast_Score=533, Evalue=1e-151,
Organism=Homo sapiens, GI38202255, Length=613, Percent_Identity=44.0456769983687, Blast_Score=529, Evalue=1e-150,
Organism=Homo sapiens, GI20070344, Length=629, Percent_Identity=41.4944356120827, Blast_Score=487, Evalue=1e-137,
Organism=Escherichia coli, GI1788013, Length=606, Percent_Identity=48.1848184818482, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1786392, Length=134, Percent_Identity=29.8507462686567, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=613, Percent_Identity=43.5562805872757, Blast_Score=524, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71984192, Length=613, Percent_Identity=43.5562805872757, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6322112, Length=625, Percent_Identity=43.04, Blast_Score=528, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6322654, Length=387, Percent_Identity=41.6020671834625, Blast_Score=319, Evalue=7e-88,
Organism=Drosophila melanogaster, GI24583842, Length=609, Percent_Identity=44.1707717569787, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI28574141, Length=609, Percent_Identity=44.1707717569787, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24583839, Length=609, Percent_Identity=44.1707717569787, Blast_Score=540, Evalue=1e-153,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT2_BACSU (P18256)

Other databases:

- EMBL:   M36593
- EMBL:   AL009126
- EMBL:   Z80360
- PIR:   A37770
- RefSeq:   NP_391636.2
- ProteinModelPortal:   P18256
- SMR:   P18256
- EnsemblBacteria:   EBBACT00000001943
- GeneID:   937135
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37560
- NMPDR:   fig|224308.1.peg.3762
- GenoList:   BSU37560
- GeneTree:   EBGT00050000001167
- HOGENOM:   HBG352811
- PhylomeDB:   P18256
- ProtClustDB:   PRK12444
- BioCyc:   BSUB:BSU37560-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73389; Mature: 73257

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHVHIQLPDGQIQEYPKGITIKEAAGSISSSLQKKAAAGQVNGKLVDLSFKLEEDAEL
CCCCEEEECCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCE
SIVTLDSQEGLQVLRHTTAHVLAQAVKRLYGEVSLGVGPVILDGFYYDMKLGKSLASGDL
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCHHHHHHCCHHHCCCH
EAIEKEMKNIINENLEIKRIEVSYEEAEELFAQKDERLKLEILKDIPRGEDITLYQQGEF
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCEEHHHHHCCCCCCCEEEEECCCC
VDLCRGPHLPSTGMIKAFKLTRVSGAYWRGDSKNEVLQRVYGVAFQKKKDLDAHLHMLEE
HHHHCCCCCCCCCCEEEEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AAKRDHRKLGKQLGLFMFSEEAPGMPFYLPKGQIVRNELERFSRELQTNAGYDEVRTPFM
HHHHHHHHHHHHHCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHH
MNQRLWEQSGHWDHYRDNMYFSEVDDTRFAMKPMNCPGHMLIFKNSLYSYRDLPIRMAEF
HHHHHHHCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCEEEEECCCCCCCCCCEEHHHH
GQVHRHEYSGALNGMLRVRTFCQDDAHIFVREDQIESEIKEAIRLIDEVYRTFGFEYSVE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
LSTRPEDSLGDDSLWEASERALARVLEELGLSYEINEGDGAFYGPKIDFHIKDALKRSHQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEHHHHHHCCCC
CATIQLDFQMPEKFDLTYINELNEKVRPVVIHRAVFGSIDRFFGILIEHYGGAFPVWLAP
EEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IQVQIIPVSHVHLDYCRKVQAELKQAGIRAGIDERNEKLGYKIRESQVQKIPYVLVLGDH
EEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHCCEEEEECCC
EEQENAVNVRRFGHQQNEHVPFQTFKDKLVKQVENRGM
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKHVHIQLPDGQIQEYPKGITIKEAAGSISSSLQKKAAAGQVNGKLVDLSFKLEEDAEL
CCCEEEECCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCE
SIVTLDSQEGLQVLRHTTAHVLAQAVKRLYGEVSLGVGPVILDGFYYDMKLGKSLASGDL
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCHHHHHHCCHHHCCCH
EAIEKEMKNIINENLEIKRIEVSYEEAEELFAQKDERLKLEILKDIPRGEDITLYQQGEF
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCEEHHHHHCCCCCCCEEEEECCCC
VDLCRGPHLPSTGMIKAFKLTRVSGAYWRGDSKNEVLQRVYGVAFQKKKDLDAHLHMLEE
HHHHCCCCCCCCCCEEEEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AAKRDHRKLGKQLGLFMFSEEAPGMPFYLPKGQIVRNELERFSRELQTNAGYDEVRTPFM
HHHHHHHHHHHHHCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHH
MNQRLWEQSGHWDHYRDNMYFSEVDDTRFAMKPMNCPGHMLIFKNSLYSYRDLPIRMAEF
HHHHHHHCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCEEEEECCCCCCCCCCEEHHHH
GQVHRHEYSGALNGMLRVRTFCQDDAHIFVREDQIESEIKEAIRLIDEVYRTFGFEYSVE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
LSTRPEDSLGDDSLWEASERALARVLEELGLSYEINEGDGAFYGPKIDFHIKDALKRSHQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEHHHHHHCCCC
CATIQLDFQMPEKFDLTYINELNEKVRPVVIHRAVFGSIDRFFGILIEHYGGAFPVWLAP
EEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IQVQIIPVSHVHLDYCRKVQAELKQAGIRAGIDERNEKLGYKIRESQVQKIPYVLVLGDH
EEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHCCEEEEECCC
EEQENAVNVRRFGHQQNEHVPFQTFKDKLVKQVENRGM
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115870; 9384377; 9353933; 1379177