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The map label for this gene is pyrG

Identifier: 16080768

GI number: 16080768

Start: 3810693

End: 3812300

Strand: Reverse

Name: pyrG

Synonym: BSU37150

Alternate gene names: 16080768

Gene position: 3812300-3810693 (Counterclockwise)

Preceding gene: 16080769

Following gene: 16080766

Centisome position: 90.43

GC content: 47.01

Gene sequence:

>1608_bases
ATGACGAAATATATTTTTGTAACCGGGGGAGTTGTATCCTCACTTGGAAAGGGTATTGTAGCAGCTTCATTAGGGCGTTT
GCTGAAAAACCGCGGACTGAACGTGACGATTCAAAAATTCGATCCATACATCAACGTTGACCCGGGAACAATGAGCCCGT
ACCAGCACGGTGAAGTATTCGTAACGGATGACGGCGCTGAAACAGACTTGGACCTTGGTCACTATGAGCGTTTTATCGAC
ATTAACCTGAATAAATTCAGCAACGTGACAACAGGTAAAATCTATTCAACAGTTCTTAAAAAAGAACGCCGCGGAGATTA
CCTTGGAGGAACCGTACAGGTCATTCCGCACATCACAAACGAATTGAAAGACCGTGTTTACCGCGCAGGGAAAGAGACAA
ACGCAGATGTTGTCATTACAGAAATCGGCGGTACTGTCGGAGACATCGAATCACTGCCATTCCTTGAAGCGATCCGCCAA
ATGAAGAGCGACATCGGCCGTGAAAACGTAATGTACATCCACTGTACGCTCGTGCCTTACATTAAAGCTGCTGGCGAATT
AAAAACAAAACCGACACAGCACAGCGTAAAAGAATTGCGCAGCTTGGGCATTCAGCCAAACATCATCGTCGTTCGTACAG
AAATGCCGATTTCTCAAGATATGAAAGATAAAATCGCGCTTTTCTGCGACATCGATACAAAAGCGGTTATTGAATGTGAA
GATGCGGACAACCTTTACTCCATTCCGCTGGAGCTTCAAAAACAAGGGCTTGATAAGCTTGTTTGCGAGCACATGAAATT
GGCATGCAAAGAAGCGGAAATGTCCGAGTGGAAAGAGCTTGTTAACAAAGTCAGCAACCTGTCTCAAACGATTACAATCG
GCCTTGTCGGCAAATACGTTGAGCTTCCTGACGCATACATTTCTGTTGTCGAGTCTCTCCGCCATGCGGGCTATGCGTTT
GACACAGATGTAAAAGTGAAGTGGATCAACGCGGAAGAAGTGACAGAAAACAATATCGCAGAACTCACAAGCGGAACAGA
CGGCATCATTGTGCCGGGCGGATTCGGAGACCGCGGTGTTGAAGGGAAAATCGTCGCGACAAAATACGCGCGCGAAAACA
ACATTCCGTTCTTGGGCATTTGCTTGGGCATGCAGGTGGCGTCTATTGAATACGCACGAAACGTATTGGGCTTAAAAGGC
GCTCACTCAGCGGAAATTGATCCGTCAACTCAATACCCGATCATTGATCTTCTTCCTGAACAAAAGGATGTTGAGGATCT
TGGGGGAACGCTTCGTCTCGGCCTTTACCCTTGTAAGCTTGAAGAAGGCACGAAAGCCTTTGAAGTGTATCAGGATGAAG
TGGTGTACGAGCGCCACCGCCACCGCTATGAGTTTAACAATGAATTCAGACAGCAAATGGAAGAGCAAGGCTTCGTATTC
TCAGGCACAAGCCCTGATGGACGCCTTGTTGAAATCATCGAGCTGAAAGACCACCCTTGGTTCGTGGCTTCTCAGTTCCA
CCCAGAGTTCAAGTCAAGACCGACAAGACCTCAGCCTCTATTCAAAGGCTTTATCGGAGCGTCTGTCGAAGCTGCAAATC
AGAAGTAA

Upstream 100 bases:

>100_bases
CCCCTCATTTTTCTTATAAATCATTTAAAAGGCTAAATGATTTTTTATAAAATAGTACATTTTAAAAATGTAGCTAAGAG
CGAAGAGAGGAGCAAACGAA

Downstream 100 bases:

>100_bases
TAAAAAAGACTTGCCGTTTGTCAAAAGCGGGCAAGTCTTTTTTATTTGTTTCCGGCAAGTTCCAGCATGTACAATAAGAT
ATTATGCATACTCTTGCAGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLNKFSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITNELKDRVYRAGKETNADVVITEIGGTVGDIESLPFLEAIRQ
MKSDIGRENVMYIHCTLVPYIKAAGELKTKPTQHSVKELRSLGIQPNIIVVRTEMPISQDMKDKIALFCDIDTKAVIECE
DADNLYSIPLELQKQGLDKLVCEHMKLACKEAEMSEWKELVNKVSNLSQTITIGLVGKYVELPDAYISVVESLRHAGYAF
DTDVKVKWINAEEVTENNIAELTSGTDGIIVPGGFGDRGVEGKIVATKYARENNIPFLGICLGMQVASIEYARNVLGLKG
AHSAEIDPSTQYPIIDLLPEQKDVEDLGGTLRLGLYPCKLEEGTKAFEVYQDEVVYERHRHRYEFNNEFRQQMEEQGFVF
SGTSPDGRLVEIIELKDHPWFVASQFHPEFKSRPTRPQPLFKGFIGASVEAANQK

Sequences:

>Translated_535_residues
MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLNKFSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITNELKDRVYRAGKETNADVVITEIGGTVGDIESLPFLEAIRQ
MKSDIGRENVMYIHCTLVPYIKAAGELKTKPTQHSVKELRSLGIQPNIIVVRTEMPISQDMKDKIALFCDIDTKAVIECE
DADNLYSIPLELQKQGLDKLVCEHMKLACKEAEMSEWKELVNKVSNLSQTITIGLVGKYVELPDAYISVVESLRHAGYAF
DTDVKVKWINAEEVTENNIAELTSGTDGIIVPGGFGDRGVEGKIVATKYARENNIPFLGICLGMQVASIEYARNVLGLKG
AHSAEIDPSTQYPIIDLLPEQKDVEDLGGTLRLGLYPCKLEEGTKAFEVYQDEVVYERHRHRYEFNNEFRQQMEEQGFVF
SGTSPDGRLVEIIELKDHPWFVASQFHPEFKSRPTRPQPLFKGFIGASVEAANQK
>Mature_534_residues
TKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDI
NLNKFSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITNELKDRVYRAGKETNADVVITEIGGTVGDIESLPFLEAIRQM
KSDIGRENVMYIHCTLVPYIKAAGELKTKPTQHSVKELRSLGIQPNIIVVRTEMPISQDMKDKIALFCDIDTKAVIECED
ADNLYSIPLELQKQGLDKLVCEHMKLACKEAEMSEWKELVNKVSNLSQTITIGLVGKYVELPDAYISVVESLRHAGYAFD
TDVKVKWINAEEVTENNIAELTSGTDGIIVPGGFGDRGVEGKIVATKYARENNIPFLGICLGMQVASIEYARNVLGLKGA
HSAEIDPSTQYPIIDLLPEQKDVEDLGGTLRLGLYPCKLEEGTKAFEVYQDEVVYERHRHRYEFNNEFRQQMEEQGFVFS
GTSPDGRLVEIIELKDHPWFVASQFHPEFKSRPTRPQPLFKGFIGASVEAANQK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=45.8029197080292, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI28559085, Length=552, Percent_Identity=45.4710144927536, Blast_Score=491, Evalue=1e-139,
Organism=Homo sapiens, GI28559083, Length=552, Percent_Identity=45.4710144927536, Blast_Score=491, Evalue=1e-139,
Organism=Homo sapiens, GI221316689, Length=552, Percent_Identity=45.4710144927536, Blast_Score=491, Evalue=1e-139,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=57.6994434137291, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI25148299, Length=606, Percent_Identity=39.2739273927393, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=44.6236559139785, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=44.8214285714286, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24664469, Length=553, Percent_Identity=46.6546112115732, Blast_Score=507, Evalue=1e-144,
Organism=Drosophila melanogaster, GI21357815, Length=498, Percent_Identity=44.578313253012, Blast_Score=430, Evalue=1e-120,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59719; Mature: 59588

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKFSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
ELKDRVYRAGKETNADVVITEIGGTVGDIESLPFLEAIRQMKSDIGRENVMYIHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHH
IKAAGELKTKPTQHSVKELRSLGIQPNIIVVRTEMPISQDMKDKIALFCDIDTKAVIECE
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEC
DADNLYSIPLELQKQGLDKLVCEHMKLACKEAEMSEWKELVNKVSNLSQTITIGLVGKYV
CCCCEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEEECHH
ELPDAYISVVESLRHAGYAFDTDVKVKWINAEEVTENNIAELTSGTDGIIVPGGFGDRGV
HCCHHHHHHHHHHHHCCCEECCCCEEEEECHHHHCCCCHHHHCCCCCCEEECCCCCCCCC
EGKIVATKYARENNIPFLGICLGMQVASIEYARNVLGLKGAHSAEIDPSTQYPIIDLLPE
CCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCC
QKDVEDLGGTLRLGLYPCKLEEGTKAFEVYQDEVVYERHRHRYEFNNEFRQQMEEQGFVF
CCCHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEE
SGTSPDGRLVEIIELKDHPWFVASQFHPEFKSRPTRPQPLFKGFIGASVEAANQK
ECCCCCCCEEEEEEECCCCEEEEECCCCHHHCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKFSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
ELKDRVYRAGKETNADVVITEIGGTVGDIESLPFLEAIRQMKSDIGRENVMYIHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHH
IKAAGELKTKPTQHSVKELRSLGIQPNIIVVRTEMPISQDMKDKIALFCDIDTKAVIECE
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEC
DADNLYSIPLELQKQGLDKLVCEHMKLACKEAEMSEWKELVNKVSNLSQTITIGLVGKYV
CCCCEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEEECHH
ELPDAYISVVESLRHAGYAFDTDVKVKWINAEEVTENNIAELTSGTDGIIVPGGFGDRGV
HCCHHHHHHHHHHHHCCCEECCCCEEEEECHHHHCCCCHHHHCCCCCCEEECCCCCCCCC
EGKIVATKYARENNIPFLGICLGMQVASIEYARNVLGLKGAHSAEIDPSTQYPIIDLLPE
CCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCC
QKDVEDLGGTLRLGLYPCKLEEGTKAFEVYQDEVVYERHRHRYEFNNEFRQQMEEQGFVF
CCCHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEE
SGTSPDGRLVEIIELKDHPWFVASQFHPEFKSRPTRPQPLFKGFIGASVEAANQK
ECCCCCCCEEEEEEECCCCEEEEECCCCHHHCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA