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The map label for this gene is rpoE

Identifier: 16080769

GI number: 16080769

Start: 3812542

End: 3813063

Strand: Reverse

Name: rpoE

Synonym: BSU37160

Alternate gene names: 16080769

Gene position: 3813063-3812542 (Counterclockwise)

Preceding gene: 16080770

Following gene: 16080768

Centisome position: 90.45

GC content: 40.61

Gene sequence:

>522_bases
TTGGGTATCAAACAATATTCACAGGAAGAGCTAAAGGAAATGGCTTTAGTTGAAATCGCTCACGAATTATTTGAAGAACA
TAAAAAACCAGTTCCTTTTCAGGAGCTTTTAAATGAAATCGCATCTTTGCTCGGCGTGAAAAAAGAAGAGCTTGGAGACC
GCATTGCTCAATTTTATACAGATTTAAACATTGACGGCCGCTTCCTGGCGCTTTCTGACCAGACGTGGGGGCTTCGCAGC
TGGTATCCTTATGATCAGCTTGATGAAGAAACTCAGCCGACAGTCAAGGCGAAAAAGAAAAAAGCGAAGAAAGCAGTCGA
AGAAGATCTTGATCTTGACGAGTTTGAAGAGATCGACGAAGACGACCTTGATTTGGATGAAGTTGAGGAAGAACTCGATC
TTGAAGCCGACGATTTTGACGAAGAAGATCTTGATGAAGACGACGATGATCTTGAGATCGAAGAAGATATTATTGATGAA
GATGATGAAGACTATGATGATGAAGAAGAGGAAATTAAATAG

Upstream 100 bases:

>100_bases
GTTTATAATGAGAGGTATGGTTTGTATAAGAACGATACAAAATCGCTTTCATTATTGATAAAACAGATACATATAAGTAA
TAGAAAGGGAGTGTCCGACC

Downstream 100 bases:

>100_bases
TATCAGATTTCATCTTCAAAACGATCTTGACTTTCATAGTCGAACTATGTAGTATGTATTTTGGGCTCTTCAAAAACGAA
GAGAGAACATAGTACGTTAT

Product: DNA-directed RNA polymerase subunit delta

Products: diphosphate; RNAn+1

Alternate protein names: RNAP delta factor

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRS
WYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDE
DDEDYDDEEEEIK

Sequences:

>Translated_173_residues
MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRS
WYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDE
DDEDYDDEEEEIK
>Mature_172_residues
GIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSW
YPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDED
DEDYDDEEEEIK

Specific function: Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis

COG id: COG3343

COG function: function code K; DNA-directed RNA polymerase, delta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rpoE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOE_BACSU (P12464)

Other databases:

- EMBL:   M21677
- EMBL:   Z49782
- EMBL:   AL009126
- EMBL:   M22039
- PIR:   JT0302
- RefSeq:   NP_391597.1
- PDB:   2KRC
- PDBsum:   2KRC
- ProteinModelPortal:   P12464
- EnsemblBacteria:   EBBACT00000003208
- GeneID:   937046
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37160
- NMPDR:   fig|224308.1.peg.3723
- GenoList:   BSU37160
- GeneTree:   EBGT00050000000191
- OMA:   TDMNTDG
- ProtClustDB:   PRK02363
- BioCyc:   BSUB:BSU37160-MONOMER
- HAMAP:   MF_00357
- InterPro:   IPR007759
- PANTHER:   PTHR23405:SF1

Pfam domain/function: PF05066 RNA_pol_delta

EC number: 2.7.7.6

Molecular weight: Translated: 20399; Mature: 20268

Theoretical pI: Translated: 3.62; Mature: 3.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDE
CCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCC
DDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCC
>Mature Secondary Structure 
GIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDE
CCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCC
DDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2843435; 9353933; 9384377; 2457578; 7545758; 10336502