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The map label for this gene is fbaA

Identifier: 16080765

GI number: 16080765

Start: 3808512

End: 3809369

Strand: Reverse

Name: fbaA

Synonym: BSU37120

Alternate gene names: 16080765

Gene position: 3809369-3808512 (Counterclockwise)

Preceding gene: 16080766

Following gene: 255767789

Centisome position: 90.36

GC content: 43.94

Gene sequence:

>858_bases
ATGCCTTTAGTTTCTATGACGGAAATGTTGAATACAGCTAAAGAAAAAGGGTACGCTGTAGGACAATTTAACTTAAACAA
CCTTGAGTTTACTCAAGCGATTTTACAAGCAGCTGAAGAAGAAAAATCTCCAGTGATCCTTGGTGTTTCCGAAGGTGCGG
GACGCTACATGGGCGGCTTCAAAACAGTCGTTGCTATGGTAAAAGCACTTATGGAAGAGTACAAAGTGACAGTTCCTGTT
GCGATTCACTTAGACCACGGTTCAAGCTTTGAATCTTGTGCGAAAGCGATTCATGCAGGTTTCACATCTGTAATGATCGA
CGCTTCTCACCATCCATTTGAAGAAAACGTAGCAACAACTGCTAAAGTTGTTGAGCTTGCTCATTTCCACGGAGTATCTG
TAGAAGCTGAGCTTGGAACAGTAGGCGGACAAGAAGATGACGTTATCGCTGAAGGCGTAATCTACGCTGATCCTAAAGAG
TGCCAAGAGCTTGTTGAGCGCACTGGCATCGACTGCCTTGCACCTGCATTAGGTTCTGTTCACGGCCCTTACAAAGGTGA
GCCAAACCTTGGCTTCAAAGAAATGGAAGAGATCGGAAAATCAACTGGCCTTCCGCTAGTTCTTCACGGCGGTACTGGAA
TTCCGACTGCTGACATCAAAAAATCAATTTCACTTGGTACAGCTAAAATCAACGTGAATACTGAAAACCAAATTTCTTCT
GCAAAAGCTGTTCGCGAAACATTGGCAGCTAAGCCTGACGAGTACGATCCACGTAAATACCTTGGACCAGCTCGTGAAGC
GATCAAAGAAACAGTTATCGGTAAAATGCGTGAATTTGGTTCTTCAAACCAAGCTTAA

Upstream 100 bases:

>100_bases
GATCTGAGTATTTACATATGACAGCAATATATGGGTCATGCTAGGGTGGAAAGCTTTTTTCGCTAGAAGACAATCAGGCT
ACATAAGGAGGACATTCGAC

Downstream 100 bases:

>100_bases
TTCAATTGGAACTTTTTATAGCCGCCTGACAGCTTGACAGGCGGTTTTCCGTCTATCTTTAGAAAGCGCAATCATATTCG
CAGTTCAGAAAATTTCTTAA

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPLVSMTEMLNTAKEKGYAVGQFNLNNLEFTQAILQAAEEEKSPVILGVSEGAGRYMGGFKTVVAMVKALMEEYKVTVPV
AIHLDHGSSFESCAKAIHAGFTSVMIDASHHPFEENVATTAKVVELAHFHGVSVEAELGTVGGQEDDVIAEGVIYADPKE
CQELVERTGIDCLAPALGSVHGPYKGEPNLGFKEMEEIGKSTGLPLVLHGGTGIPTADIKKSISLGTAKINVNTENQISS
AKAVRETLAAKPDEYDPRKYLGPAREAIKETVIGKMREFGSSNQA

Sequences:

>Translated_285_residues
MPLVSMTEMLNTAKEKGYAVGQFNLNNLEFTQAILQAAEEEKSPVILGVSEGAGRYMGGFKTVVAMVKALMEEYKVTVPV
AIHLDHGSSFESCAKAIHAGFTSVMIDASHHPFEENVATTAKVVELAHFHGVSVEAELGTVGGQEDDVIAEGVIYADPKE
CQELVERTGIDCLAPALGSVHGPYKGEPNLGFKEMEEIGKSTGLPLVLHGGTGIPTADIKKSISLGTAKINVNTENQISS
AKAVRETLAAKPDEYDPRKYLGPAREAIKETVIGKMREFGSSNQA
>Mature_284_residues
PLVSMTEMLNTAKEKGYAVGQFNLNNLEFTQAILQAAEEEKSPVILGVSEGAGRYMGGFKTVVAMVKALMEEYKVTVPVA
IHLDHGSSFESCAKAIHAGFTSVMIDASHHPFEENVATTAKVVELAHFHGVSVEAELGTVGGQEDDVIAEGVIYADPKEC
QELVERTGIDCLAPALGSVHGPYKGEPNLGFKEMEEIGKSTGLPLVLHGGTGIPTADIKKSISLGTAKINVNTENQISSA
KAVRETLAAKPDEYDPRKYLGPAREAIKETVIGKMREFGSSNQA

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family

Homologues:

Organism=Escherichia coli, GI87082041, Length=287, Percent_Identity=41.4634146341463, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789526, Length=286, Percent_Identity=40.9090909090909, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1788072, Length=280, Percent_Identity=41.0714285714286, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789293, Length=326, Percent_Identity=27.3006134969325, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322790, Length=338, Percent_Identity=24.2603550295858, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALF_BACSU (P13243)

Other databases:

- EMBL:   M22039
- EMBL:   S42590
- EMBL:   Z49782
- EMBL:   AL009126
- PIR:   S55426
- RefSeq:   NP_391593.1
- ProteinModelPortal:   P13243
- SMR:   P13243
- PhosSite:   P13243
- EnsemblBacteria:   EBBACT00000000537
- GeneID:   937040
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37120
- NMPDR:   fig|224308.1.peg.3719
- GenoList:   BSU37120
- GeneTree:   EBGT00050000002241
- HOGENOM:   HBG327581
- OMA:   IGKMREF
- PhylomeDB:   P13243
- ProtClustDB:   PRK08610
- BioCyc:   BSUB:BSU37120-MONOMER
- BRENDA:   4.1.2.13
- GO:   GO:0006096
- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01859

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.13

Molecular weight: Translated: 30401; Mature: 30270

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 85-85 BINDING 50-50 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVSMTEMLNTAKEKGYAVGQFNLNNLEFTQAILQAAEEEKSPVILGVSEGAGRYMGGF
CCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
KTVVAMVKALMEEYKVTVPVAIHLDHGSSFESCAKAIHAGFTSVMIDASHHPFEENVATT
HHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHCHHHEEEECCCCCCHHHHHHH
AKVVELAHFHGVSVEAELGTVGGQEDDVIAEGVIYADPKECQELVERTGIDCLAPALGSV
HHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCEEECCHHHHHHHHHHCCCHHHHHHHHCC
HGPYKGEPNLGFKEMEEIGKSTGLPLVLHGGTGIPTADIKKSISLGTAKINVNTENQISS
CCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHH
AKAVRETLAAKPDEYDPRKYLGPAREAIKETVIGKMREFGSSNQA
HHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLVSMTEMLNTAKEKGYAVGQFNLNNLEFTQAILQAAEEEKSPVILGVSEGAGRYMGGF
CCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
KTVVAMVKALMEEYKVTVPVAIHLDHGSSFESCAKAIHAGFTSVMIDASHHPFEENVATT
HHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHCHHHEEEECCCCCCHHHHHHH
AKVVELAHFHGVSVEAELGTVGGQEDDVIAEGVIYADPKECQELVERTGIDCLAPALGSV
HHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCEEECCHHHHHHHHHHCCCHHHHHHHHCC
HGPYKGEPNLGFKEMEEIGKSTGLPLVLHGGTGIPTADIKKSISLGTAKINVNTENQISS
CCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHH
AKAVRETLAAKPDEYDPRKYLGPAREAIKETVIGKMREFGSSNQA
HHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2457578; 1640835; 9353933; 9384377; 1556067