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The map label for this gene is prmC

Identifier: 16080753

GI number: 16080753

Start: 3796217

End: 3797083

Strand: Reverse

Name: prmC

Synonym: BSU37000

Alternate gene names: 16080753

Gene position: 3797083-3796217 (Counterclockwise)

Preceding gene: 16080754

Following gene: 16080752

Centisome position: 90.07

GC content: 45.56

Gene sequence:

>867_bases
ATGAAGACGATATTCGAAGCCCTGAAATGGGCTTCTTCTTATTTAACCGAAGCGGGAAGAGAAGAAAATGCGGCCGAGCT
CCTATTGCTTTATGATACCGGAATGGAGAGAAGCAAGCTGCTCGCAAGCTTACAGGAACCGATTGGTGAAGACGAACTGT
ATCGCTTTAAGCGTCACGTTGAGATGCATAAAGAGGGCGTGCCGGTTCAATACATTATAGGAAAAGAGTTTTTTTATGGA
CGGGAATTTATGGTGAATGATGATGTGCTGATCCCGCGGCCGGAAACTGAAGAAGTTGTCTTTCATTTGCTTGAAAAATA
TCGGAGTGTTTTTTCTGAAGACGGCAAGCTCGAAGTTGTTGACGTTGGTACAGGGAGCGGCGCAATAGCCGTCACGCTTG
CGCTTGAAAACCAAAGCTTTTCTGTGTCGGCGGTCGATATTTCGAAAGAGGCGCTGCAAGTCGCCTCTGCCAATGCAGAA
AAACTCGGAGCGAATGTGCGCTTTTATCAGGGCGATCTGCTTGAGCCTTTTATTAAGGCGGGAAAAAAAGCGGATATCAT
TGTCTCAAACCCGCCATATATTTCAGAGGAAGAAATGGCTGATTTATCGGAAATTGTCCGTTTTCATGAGCCGCTGCACG
CACTCACCGATGGCGGAGACGGGCTGAAATTTTATAAGCGGTTTATGGAAGACATTCCGCTTGTTATGAAGGATAAAGTA
TTTGTCGTTTTTGAAATCGGCTGGAAACAGGGAGCGGCGGTCAAAGACCTGATCCTCAAGGCGTTTAAGGGGGCGGAAGT
GGAAGTGCTGAAAGATATTAATGGAAAAGACAGAACCATATGCGCTCTGATTCATAAAAATAAATAA

Upstream 100 bases:

>100_bases
CAAAAGCTTGATCAGATTCTTGAAGGAAAACTTGACGAAGTGGTTGAAGCGCTGATTGTTGAAGATCAGGCAAGCAAGCT
TCAGCAGTCGGAAGGTTAAG

Downstream 100 bases:

>100_bases
GCAGCTTGCTGGCGAGTGAATGGATCAAGCTGTTTAACACCAGCAAAGGGGATGTAAACATGAAAAAGGCCCTGACAGCA
GGAATCCTGCTCATTGCGAT

Product: glutamine methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKTIFEALKWASSYLTEAGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHVEMHKEGVPVQYIIGKEFFYG
REFMVNDDVLIPRPETEEVVFHLLEKYRSVFSEDGKLEVVDVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAE
KLGANVRFYQGDLLEPFIKAGKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKV
FVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK

Sequences:

>Translated_288_residues
MKTIFEALKWASSYLTEAGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHVEMHKEGVPVQYIIGKEFFYG
REFMVNDDVLIPRPETEEVVFHLLEKYRSVFSEDGKLEVVDVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAE
KLGANVRFYQGDLLEPFIKAGKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKV
FVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK
>Mature_288_residues
MKTIFEALKWASSYLTEAGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHVEMHKEGVPVQYIIGKEFFYG
REFMVNDDVLIPRPETEEVVFHLLEKYRSVFSEDGKLEVVDVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAE
KLGANVRFYQGDLLEPFIKAGKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKV
FVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=252, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1787463, Length=273, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082085, Length=265, Percent_Identity=32.8301886792453, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324266, Length=216, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582226, Length=185, Percent_Identity=32.4324324324324, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_BACSU (P45873)

Other databases:

- EMBL:   Z49782
- EMBL:   AL009126
- PIR:   S55438
- RefSeq:   NP_391581.1
- ProteinModelPortal:   P45873
- SMR:   P45873
- EnsemblBacteria:   EBBACT00000000635
- GeneID:   938511
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37000
- NMPDR:   fig|224308.1.peg.3707
- GenoList:   BSU37000
- GeneTree:   EBGT00050000000961
- HOGENOM:   HBG732041
- OMA:   ESADMAY
- PhylomeDB:   P45873
- ProtClustDB:   PRK09328
- BioCyc:   BSUB:BSU37000-MONOMER
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 32384; Mature: 32384

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00092 N6_MTASE; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: BINDING 146-146 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIFEALKWASSYLTEAGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHV
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
EMHKEGVPVQYIIGKEFFYGREFMVNDDVLIPRPETEEVVFHLLEKYRSVFSEDGKLEVV
HHHHCCCCEEEEECCHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAEKLGANVRFYQGDLLEPFIKA
EECCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHCCCHHHHCCCEEEECCCHHHHHHHC
GKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKV
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCE
FVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK
EEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKTIFEALKWASSYLTEAGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHV
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
EMHKEGVPVQYIIGKEFFYGREFMVNDDVLIPRPETEEVVFHLLEKYRSVFSEDGKLEVV
HHHHCCCCEEEEECCHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAEKLGANVRFYQGDLLEPFIKA
EECCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHCCCHHHHCCCEEEECCCHHHHHHHC
GKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKV
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCE
FVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK
EEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377