Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 16080754

GI number: 16080754

Start: 3797085

End: 3798155

Strand: Reverse

Name: prfA

Synonym: BSU37010

Alternate gene names: 16080754

Gene position: 3798155-3797085 (Counterclockwise)

Preceding gene: 16080757

Following gene: 16080753

Centisome position: 90.1

GC content: 46.41

Gene sequence:

>1071_bases
GTGTTAGACCGTTTAAAATCAATTGAAGAACGATACGAGAAATTAAATGAGCTTTTGAGCGATCCAGAAGTAGTCAACGA
TCCGAAAAAGCTAAGAGAATATTCAAAAGAGCAATCTGATATACAAGAAACAGTTGACGTATATAGACAATACAGAGATG
CGTCAGAACAGCTGGCTGATGCAAAAGCGATGCTTGAAGAGAAGCTGGATGCTGAGATGCGCGACATGGTGAAGGAAGAG
ATTTCTGAACTTCAGAAGGAAACGGAAACACTGTCAGAACGGCTGAAGGTGCTCCTGATCCCGAAAGATCCTAATGACGA
CAAAAACGTTATCATGGAGATCCGCGGGGCTGCGGGAGGCGAGGAAGCCGCTTTATTCGCGGGCAATCTGTACAGAATGT
ATTCCCGTTATGCTGAGCTTCAGGGCTGGAAAACAGAGGTCATGGAAGCGAATGTAACCGGAACGGGCGGCTACAAAGAG
ATCATCTTTATGATTACAGGAAGCGGCGCGTATTCTAAACTCAAATATGAAAACGGCGCCCACCGCGTACAGCGTGTTCC
GGAAACAGAATCAGGCGGACGCATCCATACATCCACTGCAACAGTGGCATGTCTTCCTGAGGCAGAAGAGGTAGAGGTCG
ACATCCATGAGAAGGATATCCGTGTCGATACATTTGCTTCAAGCGGACCGGGCGGACAAAGCGTTAACACAACAATGTCT
GCGGTTCGTCTGACTCACTTGCCGACAGGTGTTGTTGTATCATGCCAGGATGAAAAATCTCAAATTAAGAACAAAGAAAA
AGCAATGAAGGTTCTCCGTGCCAGAATCTATGATAAATTTCAGCAGGAAGCCCAGGCTGAATATGATCAAACACGGAAAT
CAGCAGTCGGATCTGGTGACCGCTCTGAACGTATCCGCACATACAACTTCCCGCAAAACCGTGTGACGGATCACAGAATC
GGTTTGACGATTCAAAAGCTTGATCAGATTCTTGAAGGAAAACTTGACGAAGTGGTTGAAGCGCTGATTGTTGAAGATCA
GGCAAGCAAGCTTCAGCAGTCGGAAGGTTAA

Upstream 100 bases:

>100_bases
CAGAGGGAGCTTTTCCAGCTGGGATTGAAATGCTTTTGACTGGGGTTAATCACTATACTATAATGAGAATGTTACGCACA
GGAAAACAGAGGTGAAGACC

Downstream 100 bases:

>100_bases
GATGAAGACGATATTCGAAGCCCTGAAATGGGCTTCTTCTTATTTAACCGAAGCGGGAAGAGAAGAAAATGCGGCCGAGC
TCCTATTGCTTTATGATACC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLDRLKSIEERYEKLNELLSDPEVVNDPKKLREYSKEQSDIQETVDVYRQYRDASEQLADAKAMLEEKLDAEMRDMVKEE
ISELQKETETLSERLKVLLIPKDPNDDKNVIMEIRGAAGGEEAALFAGNLYRMYSRYAELQGWKTEVMEANVTGTGGYKE
IIFMITGSGAYSKLKYENGAHRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGSGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILEGKLDEVVEALIVEDQASKLQQSEG

Sequences:

>Translated_356_residues
MLDRLKSIEERYEKLNELLSDPEVVNDPKKLREYSKEQSDIQETVDVYRQYRDASEQLADAKAMLEEKLDAEMRDMVKEE
ISELQKETETLSERLKVLLIPKDPNDDKNVIMEIRGAAGGEEAALFAGNLYRMYSRYAELQGWKTEVMEANVTGTGGYKE
IIFMITGSGAYSKLKYENGAHRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGSGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILEGKLDEVVEALIVEDQASKLQQSEG
>Mature_356_residues
MLDRLKSIEERYEKLNELLSDPEVVNDPKKLREYSKEQSDIQETVDVYRQYRDASEQLADAKAMLEEKLDAEMRDMVKEE
ISELQKETETLSERLKVLLIPKDPNDDKNVIMEIRGAAGGEEAALFAGNLYRMYSRYAELQGWKTEVMEANVTGTGGYKE
IIFMITGSGAYSKLKYENGAHRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGSGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILEGKLDEVVEALIVEDQASKLQQSEG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=44.3708609271523, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=34.9570200573066, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=40.5797101449275, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=50.5681818181818, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=35.8208955223881, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=293, Percent_Identity=37.542662116041, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=326, Percent_Identity=43.2515337423313, Blast_Score=245, Evalue=7e-66,
Organism=Drosophila melanogaster, GI19921226, Length=336, Percent_Identity=40.4761904761905, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40234; Mature: 40234

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLKSIEERYEKLNELLSDPEVVNDPKKLREYSKEQSDIQETVDVYRQYRDASEQLAD
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEEKLDAEMRDMVKEEISELQKETETLSERLKVLLIPKDPNDDKNVIMEIRGAAGG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAGNLYRMYSRYAELQGWKTEVMEANVTGTGGYKEIIFMITGSGAYSKLKYENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCEEEEEEEECCCCHHHHCCCCCH
HRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGSGD
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILEGKLDEVVEALIVEDQASKLQQSEG
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDRLKSIEERYEKLNELLSDPEVVNDPKKLREYSKEQSDIQETVDVYRQYRDASEQLAD
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEEKLDAEMRDMVKEEISELQKETETLSERLKVLLIPKDPNDDKNVIMEIRGAAGG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAGNLYRMYSRYAELQGWKTEVMEANVTGTGGYKEIIFMITGSGAYSKLKYENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCEEEEEEEECCCCHHHHCCCCCH
HRVQRVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGSGD
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILEGKLDEVVEALIVEDQASKLQQSEG
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA