Click here to switch to the map view.

The map label for this gene is pucI

Identifier: 16080700

GI number: 16080700

Start: 3752280

End: 3753752

Strand: Reverse

Name: pucI

Synonym: BSU36470

Alternate gene names: 16080700

Gene position: 3753752-3752280 (Counterclockwise)

Preceding gene: 16080701

Following gene: 16080699

Centisome position: 89.04

GC content: 48.27

Gene sequence:

>1473_bases
ATGAAATTAAAAGAGAGTCAGCAGCAATCCAACAGGCTGAGCAATGAAGATCTGGTGCCTTTGGGACAGGAAAAACGGAC
GTGGAAAGCAATGAACTTTGCCTCCATTTGGATGGGATGTATACATAACATACCGACCTACGCGACCGTGGGAGGATTAA
TTGCGATCGGCCTTTCGCCTTGGCAGGTGCTGGCGATCATTATTACGGCATCACTTATCCTATTTGGCGCTCTTGCCTTA
AACGGCCATGCGGGGACAAAATACGGGCTGCCGTTTCCAGTGATCATTCGGGCTTCTTACGGGATATACGGCGCGAATAT
CCCCGCGCTTCTAAGGGCGTTTACAGCTATCATGTGGCTTGGCATCCAGACCTTTGCGGGTAGCACGGCACTGAACATTT
TGCTTTTGAATATGTGGCCAGGTTGGGGAGAAATTGGCGGCGAGTGGAACATTCTCGGCATTCACTTGTCCGGTTTGCTT
TCCTTTGTATTCTTTTGGGCCATTCATTTACTCGTATTGCATCACGGCATGGAGTCGATTAAACGTTTTGAGGTGTGGGC
AGGGCCTTTAGTGTATCTGGTATTTGGCGGCATGGTATGGTGGGCCGTTGATATTGCTGGAGGATTGGGCCCGATATACT
CTCAACCAGGAAAGTTTCATACGTTTTCAGAAACATTCTGGCCGTTTGCTGCCGGAGTTACTGGCATCATCGGCATCTGG
GCGACATTGATCTTAAATATACCTGATTTTACACGGTTTGCTGAAACACAAAAAGAGCAAATCAAAGGACAATTTTACGG
TTTGCCGGGAACGTTTGCGCTGTTCGCATTCGCAAGTATTACGGTGACCTCCGGCTCGCAGGTCGCGTTCGGAGAGCCGA
TTTGGGATGTTGTCGATATTTTGGCGCGGTTTGATAACCCTTATGTCATCGTCTTGTCCGTGATCACACTCTGCATCGCC
ACGATCTCTGTAAATGTCGCGGCGAATATCGTATCACCCGCTTATGATATAGCGAATGCCCTGCCGAAATATATCAATTT
CAAACGTGGCAGCTTTATCACAGCGTTGCTCGCTTTATTTACGGTTCCGTGGAAGCTGATGGAGAGCGCGACAAGCGTGT
ATGCGTTTCTTGGCTTAATAGGCGGCATGCTTGGACCGGTGGCAGGCGTGATGATGGCCGACTATTTTATCATTCGCAAA
CGTGAGCTCTCGGTAGATGACCTGTATTCCGAAACAGGACGGTATGTGTATTGGAAGGGCTACAATTACCGTGCGTTTGC
AGCCACAATGTTAGGAGCGCTGATTTCGCTGATTGGCATGTATGTTCCTGTATTGAAGAGCTTATACGATATTTCTTGGT
TTGTAGGTGTGCTGATTTCCTTTCTTTTCTACATTGTTCTGATGCGTGTTCACCCGCCTGCATCATTGGCAATTGAAACA
GTTGAACATGCGCAGGTCCGCCAGGCTGAATAA

Upstream 100 bases:

>100_bases
CAGTGTTATTGTCTGACTCTTCAGTTATATTAAGTGACATCTAATGTCAAAAAACATAACGTGTGACGACATATAGACAA
TATGGAAAGGGGAGCCGGCT

Downstream 100 bases:

>100_bases
GATCTATTTAGACGGTATATTTTCATATTTTTGAAATGGTTTTCCATAAATAGAACATTTATAATGGTTTTAGAAATCTA
TTCTAAAACGAAATGGGAAT

Product: allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKLKESQQQSNRLSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSPWQVLAIIITASLILFGALAL
NGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWLGIQTFAGSTALNILLLNMWPGWGEIGGEWNILGIHLSGLL
SFVFFWAIHLLVLHHGMESIKRFEVWAGPLVYLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWPFAAGVTGIIGIW
ATLILNIPDFTRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVIVLSVITLCIA
TISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLMESATSVYAFLGLIGGMLGPVAGVMMADYFIIRK
RELSVDDLYSETGRYVYWKGYNYRAFAATMLGALISLIGMYVPVLKSLYDISWFVGVLISFLFYIVLMRVHPPASLAIET
VEHAQVRQAE

Sequences:

>Translated_490_residues
MKLKESQQQSNRLSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSPWQVLAIIITASLILFGALAL
NGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWLGIQTFAGSTALNILLLNMWPGWGEIGGEWNILGIHLSGLL
SFVFFWAIHLLVLHHGMESIKRFEVWAGPLVYLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWPFAAGVTGIIGIW
ATLILNIPDFTRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVIVLSVITLCIA
TISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLMESATSVYAFLGLIGGMLGPVAGVMMADYFIIRK
RELSVDDLYSETGRYVYWKGYNYRAFAATMLGALISLIGMYVPVLKSLYDISWFVGVLISFLFYIVLMRVHPPASLAIET
VEHAQVRQAE
>Mature_490_residues
MKLKESQQQSNRLSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSPWQVLAIIITASLILFGALAL
NGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWLGIQTFAGSTALNILLLNMWPGWGEIGGEWNILGIHLSGLL
SFVFFWAIHLLVLHHGMESIKRFEVWAGPLVYLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWPFAAGVTGIIGIW
ATLILNIPDFTRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVIVLSVITLCIA
TISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLMESATSVYAFLGLIGGMLGPVAGVMMADYFIIRK
RELSVDDLYSETGRYVYWKGYNYRAFAATMLGALISLIGMYVPVLKSLYDISWFVGVLISFLFYIVLMRVHPPASLAIET
VEHAQVRQAE

Specific function: Transport of allantoin

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family

Homologues:

Organism=Escherichia coli, GI87081749, Length=470, Percent_Identity=38.2978723404255, Blast_Score=350, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6322219, Length=503, Percent_Identity=28.4294234592445, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6319495, Length=494, Percent_Identity=27.1255060728745, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319429, Length=495, Percent_Identity=27.6767676767677, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324766, Length=460, Percent_Identity=23.9130434782609, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324645, Length=456, Percent_Identity=21.4912280701754, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323266, Length=447, Percent_Identity=21.0290827740492, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALLP_BACSU (P94575)

Other databases:

- EMBL:   Z82987
- EMBL:   AL009126
- PIR:   H70064
- RefSeq:   NP_391528.1
- ProteinModelPortal:   P94575
- SMR:   P94575
- EnsemblBacteria:   EBBACT00000001121
- GeneID:   936935
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36470
- NMPDR:   fig|224308.1.peg.3654
- GenoList:   BSU36470
- GeneTree:   EBGT00070000032308
- HOGENOM:   HBG616138
- OMA:   WFGIQTW
- PhylomeDB:   P94575
- ProtClustDB:   CLSK888025
- BioCyc:   BSUB:BSU36470-MONOMER
- InterPro:   IPR001248
- InterPro:   IPR012681
- TIGRFAMs:   TIGR00800

Pfam domain/function: PF02133 Transp_cyt_pur

EC number: NA

Molecular weight: Translated: 53983; Mature: 53983

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11bace74)-; HASH(0x12b6a1e4)-; HASH(0x12340430)-; HASH(0x117ce968)-; HASH(0x12a96d00)-; HASH(0x12dd4fe0)-; HASH(0x12c6b280)-; HASH(0x12c95a0c)-; HASH(0x12645378)-; HASH(0x12cef1c0)-; HASH(0x12e4028c)-; HASH(0x10ecc08c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKESQQQSNRLSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSP
CCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
WQVLAIIITASLILFGALALNGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
GIQTFAGSTALNILLLNMWPGWGEIGGEWNILGIHLSGLLSFVFFWAIHLLVLHHGMESI
HHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
KRFEVWAGPLVYLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWPFAAGVTGIIGIW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEECHHHHHHHHHHHHHHHHHH
ATLILNIPDFTRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDI
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCEECCCCHHHHHHH
LARFDNPYVIVLSVITLCIATISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALF
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TVPWKLMESATSVYAFLGLIGGMLGPVAGVMMADYFIIRKRELSVDDLYSETGRYVYWKG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEC
YNYRAFAATMLGALISLIGMYVPVLKSLYDISWFVGVLISFLFYIVLMRVHPPASLAIET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VEHAQVRQAE
HHHHHHHCCH
>Mature Secondary Structure
MKLKESQQQSNRLSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSP
CCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
WQVLAIIITASLILFGALALNGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
GIQTFAGSTALNILLLNMWPGWGEIGGEWNILGIHLSGLLSFVFFWAIHLLVLHHGMESI
HHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
KRFEVWAGPLVYLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWPFAAGVTGIIGIW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEECHHHHHHHHHHHHHHHHHH
ATLILNIPDFTRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDI
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCEECCCCHHHHHHH
LARFDNPYVIVLSVITLCIATISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALF
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TVPWKLMESATSVYAFLGLIGGMLGPVAGVMMADYFIIRKRELSVDDLYSETGRYVYWKG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEC
YNYRAFAATMLGALISLIGMYVPVLKSLYDISWFVGVLISFLFYIVLMRVHPPASLAIET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VEHAQVRQAE
HHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136